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The number of testicular cancer cases grows every year. The prevention is very important for early diagnosis. The aim of this thesis was to survey the awareness of testicular cancer prevention in men in South Bohemia and the Vysočina Region and their attitude to a testicular self-examination. The empirical part describes a research which was focused on men aged between 30 to 50 who live in the South Bohemian and Vysočina region. To collect information, a quantity survey in the form of questionnaire was used. Four hypothesis had been set for this study. Based on the data analysis, other interesting relations were studied. To evaluate the hypothesis, the Kruskal-Wallis test was used. Thanks to the studied hypothesis it was learnt that there is not a statistically significant difference in the knowledge of men in the South Bohemian and Vysočina region of the testicular cancer prevention depending on the age or the highest level of education. Later on there was also learnt that there is not a statistically significant difference in the attitude of men in the South Bohemian and Vysočina region depending on age or the highest level of education. The study also revealed that there is not a statistically significant difference in the attitude of men in the South Bohemian and Vysočina region to the testicular self-examination depending on age or the highest level of education. According to the questionnaire survey we also found out if men undergo preventive medical examination, whether they had been informed about the testical self-exam, if they examine themselves, or, where they get information about these issues. This thesis can be also used as a feedback for general practitioners and nurses working in the primary care because it gives them idea how much these men in the South Bohemian and Vysočina region are aware about testicular tumors and their prevention.