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The aim of the thesis was to verify use of peracetic acid (PAA) in production conditions for ectoparasites control in carp pond farming. First test was about evaluation of organic load in pond water on speed PAA decay in two temperature regime (11 15 and 21 °C). Results showed that between 3 6 hours from PAA application the concentration of PAA was under a half of the initial concentration (6 mg.l-1). Also statically important differences (p= < 0.05) were found in speed of decay between samples with organic load (3 samples from ponds) and samples without organic load (distilled and tap water) in both temperature regimes. Application of PAA during the transport of fish into the transport boxes was performed in three age categories (1, 2 and 3 years old common carps), where the fish were exposed for 60 90 minutes in 1.5 mg.l-1 PAA as Persteril 36. Parasitic examinations of skin and gills showed reduction of number protozoal parasites (Trichodina spp. and Apiosoma spp.) in individuals comparing with control. Against other detected parasites was PAA ineffective. Statistical evaluation showed significant (p=

The aim of the present study was to assess an influence of two different therapeutical concentrations of peracetic acid on selected haematological and biochemical parameters in grass carp (Ctenopharyngodon idella). Fish were radomly distributed to aquaria and exposed to concentrations of 0 mg.l-1 PAA (control group), 1.0 mg.l-1 PAA (P1 group), 3.0 mg.l-1 PAA (P2 group). Almost total mortality of fish was observed in the concentration 3.0 mg.l-1 PAA during the treatment comparing with the P1 group and untreated control where no mortality was observed. After the end of the experimental exposure of fish to peracetic acid, the sampling of blood has been realised. The samples of the blood were examined in order to determine haematological and biochemical parameters. Consequently, there were no significant differences (p

The aim of the diploma thesis was to evaluate the influence of antiparasitic baths on selected biochemical and haematological parameters in the blood samples of fish. According to the results the application schema will be specified or amended such alterations will be substantiated. A test was performed on the fry of carp (naked and scaly form) to evaluate the influence of the peractetic acid with the help of biochemical and haematological parameters and with the histopathological examination. The common carp was exposed to the concetration 1 mg?l-1 and 2 mg?l-1 of PAA. Three days after application was taken the blood samples from fish and the biochemical and haematological parameters were determined. Than was taken a sample of skin with the musculature from fish to be sent for the histopathological examination in University of Veterinary and Pharmaceutical Sciences in Brno. Based on the results the naked form of the carp has been declared to be more sensitive to the application of PAA. All the observed changes in haematological and biochemical parameters were within physiological range, so it can be stated, that the application schema of PAA has not a signifiant negative influence on the health of the tested fish.

Using the method of bacterial carriers, the devitalizing effect was studied of Persteril and triethylene glycol vapours, acting in vitro, on a 24-hour culture of a mixture of 39 strains of Bordetella bronchiseptica, isolated from the nasal cavity of pigs which came from five stocks suffering from the clinical form atrophic rhinitis. After exposure of the carriers of Bordetella bronchiseptica to the action of spontaneously developing vapours o the mentioned chemicals, the devitalizing effect was determined by bacteriological examination. The vapours of the water solution of Persteril, containing 0.1% of peracetic acid, showed a 90.91 to 93.83% devitalizing effect after 48-hour exposure at room temperature. When the vapours of warmed concentrated triethylene glycol were used, a 98.57 to 100% devitalizingeff ect was obtained after a 72-hour exposure under the same temperature conditions. In experiements performed at a temperature of 37 degrees C, a 24-hour exposure sufficed for obtaining 91.67 to 100% devitalizing effectiveness. There is a discussion concerning the importance of the obtained results and the need for testing them experimentally by a methodically simple and cheap continual disinfection of the air and stable surfaces in premises for pig stocks affected by atrophic rhinitis.