Abandoned industrial-waste deposits (incl. fly ash or tailings ponds) represent very specific and mostly extreme habitat conditions. They offer unique opportunity to study primary succession in cultural landscape. Substrate of the material deposited here shows extreme properties, such as low pH, overheating of the open surface, salinization etc. The aim of this work is to map the colonization of different successional stages of vegetation by ants after years of abandonment of ore-waste deposits in Chvaletice (Eastern Bohemia, CR) and to compare the present state with analogous study made in 2001. Particular aims of both studies (Jarešová 2001, and present study, 2011-12) is to test the influence of ants on vegetation succession. During the years 2011-2012 several prospections were made to record species diversity of ants. On the surface plateau of the sedimentation basin two types of habitats were chosen - the areas with present ant nests and the other areas without them. Each of both types of habitats exhibits different plant species richness: separately the species-area curves were constructed and explained. Three dominant ant species of different size categories present on sedimentation basin were selected for experimental offering of plant seeds, Formica pratensis, Lasius niger, Tetramorium...