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This diploma thesis is focused on cost index. Cost index is one of the method, which is used for cost optimalization in airline operation. Cost index compare time-related costs during flight and cost of fuel. Value of cost index influences cruise speed and fuel consumption, the higher cost index, the higher speed. Costs classification into fixed, variable and time-related costs is very important for cost index calculation. Goal of thesis is decribe importance of using cost index and advantages resulting from correct cost index application in airline operations. Practical part is focued on fuel consumption of Boeing 737 on specific routes and its evaluation. The goal is to compare calculated and real fuel consumption and find, if an airline is able to achieve calculated consumption in real operation.