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2. [Determination of barbiturates from biological material].

Author

Pracharová M, Pestálovă M, Dubský H, and Hána K

Subjects
Amobarbital metabolism, Barbital metabolism, Barbiturates blood, Barbiturates urine, Chromatography, Gas, Forensic Medicine, Hexobarbital metabolism, Humans, Hydrogen-Ion Concentration, Pentobarbital metabolism, Phenobarbital metabolism, Barbiturates metabolism
Abstract

A trace quantitative analysis of barbiturates has been carried out in blood, urine, organs and in gastric and intestinal concents. The amount of sample required for analysis is very small [approximately 400 mul blood]. Extraction is carried out four times with the mixture of acetone and either 1:1]. The preparation of columns, packing and standards has been described. The device used for the analyses [Chrom 3] is furnished with an adjusted feeding block preventing the decomposition of samples in the doser. The column temperature is 190 degrees C, the column packing is Chromaton N-AW-DMCS coated with 3% NPGS and 0.75% trimer acid, detector FID.

Published
1975

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