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This paper aim was to adapt a physiological parameter which reflects plant growth and productivity for wheat plants in relation with the applied nitrogen dose. This index can be used for various plants due to the fact that can be easy calculated and gives information about the status and requirements of diverse crops. Nitrogen is the most widely used chemical fertilizer and the adjustment of the dose is really important for farmers, who are searching for an optimum cost-benefit ratio. For this study, five experimental N doses were utilized, between 0 and 200 kg/ha. Experimental field was located within Didactic Station of BUASMV Timisoara, Romania. Nitrogen supply index was determined for healthy leaves and mathematical models were developed. The optimum N amount used for this study was 119 kg/ha and the analysis of differences reflected the modifications for doses ranging between 0 and 200 kg/ha.