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Potaknuti podacima i spoznajom da akutne odontogene infekcije maksilofacijalne regije još i danas u eri vrlo razvijene antibakterijske kemoterapije predstavljaju velik problem, željeli smo odrediti pogodan način uzimanja uzorka za mikrobiološku pretragu i određivanje antibiograma kod akutnih odontogenih infekcija. Ispitivanju je podvrgnuto dvadeset četiri bolesnika s odontogenim apscesima koji prethodno nisu bili tretirani niti medikamentno niti kirurški. Uzorci su uzeti punkcijom u količini od 0,5 do 1 ccm3 i stavljeni u transportnu hranjivu podlogu, te pomoću sterilne eze (putem brisa). Nasađivani su na hranilišta u roku od 4, 24 i 48 sati nakon uzimanja. U tvrdili smo da je uzimanje uzorka punkcijom u količini od 0,5 do 1 ccm gnjojnog sadržaja i inokulacija uzetog uzorka u transportnu hranjivu podlogu pogodan način, jer se tako osjetljive bakterijske vrste očuvaju i do 48 sati nakon uzimanja uzorka., We embarked upon this study to find an appropriate method of sampling for microbiological testing and determination of antibiogram in acute odontogenic infections, of maxillofacial region since they have been documented to cause considerable problems, in face of advanced antibacterial chemotherapy. Twenty-four patients with odontogenic abscesses, not previously treated either medicinally or surgically, were included in the study. Samples of 0.5 to 1 ccm3 were taken either biopsy and placed in a nutrient transport medium, or by means of a sterile wire loop (by a swab). They were cultured in a medium with in 4, 24 and 48 h after sampling. Sampling of 0.5 to 1 ccm 3 of the purulent matter and its inoculation in a nutrient transport medium was found to be quite appropriate, since sensitive strains of bacteria can thus be preserved up to 48 h after sampling. Mixed aerobic-anaerobic cultures were found to prevail in the samples under study.

U radu smo ispitivali šezdeset i tri bolesnika s odontogenim apscsima, koji prethodno nisu bili tretirani niti medikamentno niti kirurški. Uzorci za mikrobiološku pretragu uzimani su iz apscesa ispitivanih bolesnika u količini od 0,5—1 ccm gnojnog sadržaja, te nasađivani na po­godna hranilišta za aerobe i anaerobe. Na taj način očuvane su na životu gotovo sve vrste aerobnih i anaerobnih bakterija koje su bile zastupljene u ispitivanim uzorcima. Mikrobiološkom obradom utvrđeno je da u ispitivanim uzorcima pre­vladavaju miješane aerobno-anaerobne kulture. Među aerobnnim bakte­rijskim vrstama dominiraju gram pozitivni koki i to alfa-hemolitički streptokoki. Od anaeroba najzastupljeniji su bili gram-negativni štapići (Bacteroides melaninogenicus i Fusobacterium nucleatum) i gram-pozitivni koki anaerobnog tipa (Peptostreptococcus anaerobius i Peptococcus prevotii). Stafilokoke smo izolirali samo u tragovima., A group of 63 patients with odontogenic abscesses, not previously treated with either medication or surgery, was studied. Samples for mi­crobiology were taken from abscesses, in the amount of 0.5—1 ccm of the suppurative content, and cultured in appropriate media for aerobes, respectively, so that almost all strains of both aerobic and anaerobic bacteria present in the study samples were kept alive. Microbiological examinations revealed the mixed aerobic-anaerobic cultures to predominate in the samples uner study. Among aerobic bac­terial strains, gram-positive cocci, i. e. alpha-hemolytic streptococci, were found to prevail, whereas among anaerobes gram-negative bacilli (Bacte- riodes melaninogenicus and Fusobacterium nucleatum) and gram-positive cocci of anaerobic type (Peptostreptococcus anaerobius and Peptococcus prevotii) were most frequently detected. Staphylococci were isolated in traces only.