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Quality sportswear is evaluated through its thermal physiological and ergonomic comfort. In this paper we study the correlation of the mechanical properties of knitwear garments and the ergonomic comfort of clothes. Sportswear is made from different types of materials that can visually seem the same, but when it comes to the mechanical properties that are significantly associated with the fitting of clothes and its deformation during use, there can be significant differences between them. To create a pair of women's cycling pants, the following two kinds of knitwear with the same structural characteristics but with different raw material composition, were used. Two models of women's cycling pants were made from these kinds of knitwear. For making both models of pants, the cutting parts of the same design were used, but with different combinations of knitwear of different elasticity for individual cutting parts, for the purpose of reaching a better ergonomic comfort. The correlation of the mechanical properties of knitwear and the ergonomic comfort of women's cycling trousers was determined through trial wearing and subjective evaluation. Taking into account the study of literature that deals with the issue of comfort sportswear, the mechanical and thermal properties of the used knitwear, and finally the realized trial wearing of female cycling pants, it can be concluded that, in addition to clothing design, the mechanical properties of knitwear and extensibility of materials have a large impact on the ergonomic comfort of the used clothing. Tests have shown that a large contact pressure on certain parts of the body can be reduced by using higher tensile knitwear, as shown in this paper for Model 2. The structural model of cycling pants for Model 2 is the same, and has a significantly increasing functionality and comfort when for the side part of pants instead of ACG-S knitwear we use the ACG-C knitwear, which has a higher extensibility in the transverse direction. [ABSTRACT FROM AUTHOR]