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为提高牡丹籽油乳液的氧化稳定性，将叔丁基对苯二酚（TBHQ）与天然抗氧化剂（生育酚、抗坏血酸）进行复配，测定不同抗氧化剂对牡丹籽油乳液的抗氧化作用，并分析了不同复配抗氧化剂的相互作用机制。结果表明：在贮藏30 d时，添加和未添加抗氧化剂牡丹籽油乳液的粒径相较于0 d 均略有增加，200 mg/kg TBHQ与800 mg/kg抗坏血酸复配时乳液的Zeta电位绝对值最大，且抗氧化作用最强；TBHQ与抗坏血酸表现出较好的协同抗氧化作用，与生育酚表现出拮抗作用；TBHQ与生育酚之间拮抗作用的原因主要是TBHQ减少了生育酚在牡丹籽油乳液中油水界面的含量，而TBHQ与抗坏血酸之间协同作用主要是由于抗坏血酸增加了TBHQ在牡丹籽油乳液中油水界面的含量。综上，TBHQ与抗坏血酸复配能提高牡丹籽油乳液的氧化稳定性。In order to improve the oxidative stability of peony seed oil emulsion, tert-butylhydroquinone (TBHQ) was complexed with natural antioxidants (tocopherol and ascorbic acid), and the antioxidant effects of different antioxidants on the peony seed oil emulsion were determined and the mechanism of interaction between the different complexed antioxidants was analyzed. The results showed that the particle size of peony seed oil emulsion with and without antioxidants increased slightly at the 30 d of storage compared with that at the 0 d of storage. The absolute value of the Zeta potential of the emulsion added with 200 mg/kg TBHQ and 800 mg/kg ascorbic acid was the largest and the antioxidant effect was the strongest. TBHQ showed better synergistic antioxidant effect with ascorbic acid, and antagonistic effect with tocopherol. The antagonistic effect between TBHQ and tocopherol was mainly due to the fact that TBHQ reduced the content of tocopherol in the oil-water interface in peony seed oil emulsion, while the synergistic effect between TBHQ and ascorbic acid was mainly due to the fact that ascorbic acid increased the content of TBHQ in the oil-water interface in peony seed oil emulsion. In conclusion, the combination of TBHQ and ascorbic acid can effectively improve the oxidation stability of peony seed oil emulsion.

目前，酚类抗氧化剂TBHQ的常用检测方法存在前处理烦琐、耗时长、单次只能测定一个样品的问题，因此亟需建立一种高通量、快速准确的检测方法。为此，将高效薄层色谱(HPTLC)分离与1，1-二苯基-2-三硝基苯肼(DPPH)还原变色反应、图像像素定量分析相结合，建立了一种快速筛检酚类抗氧化剂TBHQ的方法。在此基础上，以食用油、面包和蛋糕为代表性样品，验证所建方法的实际应用性能。结果显示：所建方法检测TBHQ的检测限为70 ng/zone (35 mg/kg)，定量限为140 ng/zone (70 mg/kg)，定量限低于GB 2760—2014规定的使用限量(200 mg/kg)；通过对比分析，样品提取物中共存的背景基质对目标物定量的干扰可以忽略，证明了该方法具有良好的选择性；目标物在150～550 ng/zone范围内表现出良好的线性（R2=0.989 2）；多次平行（n=3）测试和国标规定使用限量水平附近的加标回收率测试结果证明了该方法具有较好的精密度和准确度。综上，该方法具有基质耐受性强、定量准确、简便可靠且分析通量大等优点，特别适合粮油食品中各种抗氧化剂的快速定量筛查，具有凝炼成为相关领域国家标准的潜力和价值。 At present, the common detection methods of phenolic antioxidant TBHQ have the problems of cumbersome pretreatment, long time-consuming and can only determine one sample at a time. Therefore, it is urgent to establish a high-throughput, fast and accurate detection method. A rapid method for screening phenolic antioxidant TBHQ was established by combining high performance thin layer chromatography (HPTLC) separation with reduction discoloration reaction of 1,1-diphenyl-2-trinitrophenylhydrazine (DPPH) and quantitative analysis of image pixels. On this basis, edible oil, bread and cake were taken as representative samples to verify the practical application performance of the proposed method. The results showed that the detection limit of the established method to detect TBHQ was 70 ng/zone (35 mg/kg), the limit of quantification was 140 ng/zone (70 mg/kg), and the limit of quantification was lower than the limit of use (200 mg/kg) specified in GB 2760-2014. Through comparative analysis, the interference of the background matrix coexisting in the sample extract on the target quantity could be ignored, which proved that the method had good selectivity. The target showed good linearity in the range of 150-550 ng/zone (R2=0.989 2). Secondly, several parallel tests (n=3) and the test results of spiked recovery near the specified limit level of national standard proved that the method had good precision and accuracy. In summary, this method has the advantages of strong matrix tolerance, accurate quantification, simpleness and reliableness, and large analytical flux. Therefore, it is particularly suitable for rapid quantitative screening of various antioxidant additives in cereal and oil food, and has the potential and value of becoming a national standard in related fields.

Objective: To explore the difference of therapeutic effect between tert-butylhydroquinone (tBHQ) and sulforaphane (SFN) in rats with traumatic brain injury. Methods: A total of SO healthy adult male rats were randomly divided into 4 groups: sham group. TBI group, tBHQ treatment group and SFN treatment group, then the traumatic brain injury (TBI) model were prepared by eCCI. tBHQ treatment group rats were given intraperitoneal injection three tunes tBHQ (50 mg/kg) before injury 24 h. and SFN treatment group rats were given intraperitoneally injected SFN (5 mg/kg) after injury 15 min. Neurologic deficits was evaluated 24 h after TBI by mNSS method, brain edema was detected by the wet-dry ratio method. The expression levels of NOX2 protein and Nrf2 protein in the brain tissue were investigated using Western blot and ELISA analysised. respectively. Results: Compared with TBI group, there were significant difference on mNSS scores ((4.5± 0.71) vs (9.2± 0.79). (6.0± 0.82) vs (9.2± 0.79)). brain edema (79.4% vs 85.6%. 80.3% vs 85.6%). the expression levels of NOX2 protein and Nrf2 protein (0.93 ng/mL vs 0.81 ng/mL. 0.87 ng/mL vs 0.81 ng/mL) in tBHQ treatment group and SFN treatment group. In addition, there were also significant differences on mNSS scores ((4.5± 0.71) vs (6.0± 0.82)), the expression levels of NOX2 protein and Nrf2 protein (0.93 ng/mL vs 0.87 ng/mL) between tBHQ treatment group and SFN treatment group. Conclusion: In the TBI model of rats. tBHQ and SFN could effectively attenuated cerebral oxidative stress and improved nerve function after TBI. but the curative effect of tBHQ was better than the SFN. [ABSTRACT FROM AUTHOR]

Objective: Improving lipotoxicity-induced injury in vascular endothelial cells plays a critical role in the prevention and cure of cardiovascular diseases. This study was conducted to investigate the protective role of tert-butylhydroquinone (TBHQ) against free fatty acids-induced lipotoxicity. Methods: Human umbilical vein endothelial cell Iine-Ea.hy926 was employed in this study. Cells were treated with different dose of TBHQ and/or saturated fatty acids (SFAs). The cell death was detected. We also employed western blotting and signal inhibitors to investigate the potential mechanisms. Results: SFAs significantly induced cell death in vascular endothelial cells (P<0.05). TBHQ significantly inhibited SFAs-induced cell death (P<0.05). The activation of autophagy markedly inhibited SFAs-induced lipotoxicity (P<0.05). Additionally. TBHQ significantly activated autophagy in vascular endothelial cells (P<0.05). Inhibiting autophagy markedly blocked the protective role of TBHQ against SFAs-induced cell death (P<0.05). Conclusions: TBHQ protected SFAs-induced cell injury via activating autophagy in vascular endothelial cells. This provided an alternative way in the improving or prevention and cure of hyperlipidemia-induced damage in vascular system. [ABSTRACT FROM AUTHOR]

Objective: To study the protective effects and possible mechanisms of tBHQ in rats exposed to acute silicadust. Methods: Male Wistar rats without specific pathogen SPF were randomly divided into control group、model group and intervention group, 32 rats for each group. Rats in the model group and the intervention group were treated with a single non exposed tracheal dust method to establish the rats exposed to silica model. The intervention group was intervened with 1% tBHQ solution, once a day. Each group 8 rats were sacrificed at 3,14,28,60d, respectively.The contents of IL-1,TNF-α, HYP,TGF-β in the lung tissue of rats were determined by enzyme linked immunosorbent assay(ELISA), the contents of MDA and the GSH-PX activity in lung tissue and serum of rats were determined by colorimetry. Results: Compared with the control group, the contents of IL-1 in the lung tissue of the model group and the intervention group at each time point increased with time, the contents of IL-1 of model group reached the maximum at 60d, the contents of IL-1 in intervention group reached the maximum at 28d( P <0.05), except 3 d. Compared with the control group, the contents of TGF-β and HYP in the lung tissue of the model group and the intervention group at 28 and 60 d increased with time and reached the maximum at 60d( P <0.05). Compared with the model group at each time point, the contents of IL-1, TGF-β, HYP in the intervention group were lower than those in the model group( P <0.05). Compared with the control group, the contents of MDA in lung tissue and serum of rats in model group and intervention group at different time points increased with time( P <0.05). Compared with the control group,the activity of GSH-PX in serum and lung tissue of rats in model group and intervention group decreased with time,there was no significant difference in 3d( P <0.05), compared with the control group, the activity of GSH-PX in the lung and serum tissue of in-tervenetion group was higher than model group except the 3d( P <0.05). Conclusion: The intervention of tBHQ can alleviate oxidative stress in rats exposed to silica dust to some extent, improve the antioxidant capacity of the body, and reduce the contents of IL-1, TNF-α, TGF-β,HYP in lung tissue,it has acertain impediment and inhibition effect on the inflammation and fibrosis caused by acute silica dust exposure.