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Copyright of Food Research & Development is the property of Food Research & Development Editorial Department and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

The aging and deterioration of corn seeds are the key factors that affect their vitality. Therefore, identifying the storage time of seeds is of great significance for the preservation of germplasm resources and the quality identification of seeds. This study used machine learning algorithms to identify corn seeds stored for different years by analyzing the correlation between the physicochemical parameters of 103 different corn varieties and hyperspectral bands. Hyperspectral imaging technology was employed to obtain spectral data from both sides of the seed embryonic and endosperm. The near-infrared component analyzer IM9500 and portable ultraviolet-visible fluorescence spectrometer Multi-plex were utilized to acquire the physicochemical parameters of corn seeds. Variance and linear discriminant analyses were conducted to study the trends of physicochemical parameters within corn seeds as storage time increases and their impact on the discrimination of storage time. Physicochemical parameters significantly impacting the discrimination of corn seed storage time were selected. The hyperspectral data underwent black-and-white correction, and the threshold segmentation method effectively separated the background area to obtain the Region of Interest (ROI), with the spectral average of all pixel points on the image serving as the ROI's spectral data. To eliminate interference signals such as background noise, baseline drift, and stray light in the spectral acquisition process, five preprocessing methods Savitzky-Golay (SG) smoothing, standard normal variate transformation (SNV), multiplicative scattering correction (MSC), first derivative (1-Der), and second derivative (2-Der) were applied to the spectral data. Since hyperspectral images contain many bands and redundant information, feature wavelength selection on the complete spectral data was necessary. Competitive adaptive reweighted sampling (CARS) and uninformative variable elimination (UVE) were used for feature wavelength selection. Support vector machine (SVM), backpropagation neural network (BPNN), and convolutional neural network (CNN) classification models were developed. Preprocessing and feature wavelength selection were performed on the spectral data from the embryo and endosperm surfaces. The processed spectral data were used as input for the models, and the classification results of different models were compared. The results indicated that the spectral data from the embryonic side had a better discrimination effect on the storage time of corn seeds. Modeling with preprocessed and feature-selected spectral data significantly outperformed modeling with raw data. By conducting Pearson correlation analysis between physicochemical parameters that significantly affect seed age discrimination and hyperspectral bands, the study associated these parameters with highly correlated feature bands for modeling. The study compared the modeling accuracy of feature wavelength selection, correlation coefficient selection, and the combination of correlation and feature wavelength selection. It verified the model's ability to detect across different corn varieties. The results showed that the BPNN classification model established based on the combination of correlation coefficient selection and feature wavelength selection had the highest accuracy, with a single kernel prediction accuracy of 92.3% and a Colony prediction accuracy of 94.4%. The proposed method has been validated across multiple corn varieties, demonstrating high generalization ability. This study provides a theoretical basis for the precise management of corn seed storage and holds significant practical implications for revitalizing the seed industry. [ABSTRACT FROM AUTHOR]

Copyright of Chinese Journal of Oil Crop Sciences is the property of Oil Crops Research Institute of Chinese Academy of Agricultural Sciences and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Cassava cultivation is low-cost and high-yielding, and tubers, stems and leaves can be widely utilized in the food medicine and light industry. However, cassava planting is mainly manual work at present. There is an urgent need to develop precision planting machines suitable for the agronomic requirements of cassava planting. A seeder is the core component of a precision planting machine, including the real-time and pre-cut seed-cutting type. Among them, the real-time seed cutting cannot realize the automatic sowing, due to the long and complicated shape of the cassava seed rods, while the low persistence of manual seed feeding and serious leakage. A seed cutter can be used to cut the cassava seed stems into seed stems of about 150 mm in length in pre-cut seeding. An automatic continuous and controlled seeding of cassava seed stems can be achieved through the seed discharge mechanism after cleaning. The lifting type seed rower has been improved to add the gravity seed cleaning mechanism, while the structure of the clamping plate has been optimized for the seed rowing effect. But the leakage filling still exists so far. Some research has been conducted on the seeders, such as slotted wheels, and single roller types. However, it is very necessary to improve the seed filling performance and qualification index, when seeding cassava seed stems with the existing pre-cut seed sower, because the cassava seed stems are cylindrical woody stalks of a certain length with surfaces of complex physical characteristics. Particularly, the performance of cassava precision seeding needs to be improved, due to a complex process with the multi-factor change. This study aims to improve the seed filling effect and low qualification index of pre-cut seeding type cassava precision seeder. A precision cassava seeding mechanism was designed with pre-cut seeding and friction belt, consisting of seed drop slide, active roller, type hole friction belt, support roller group, driven roller, and seed storage box. The basic structure and working principle of the seed-rowing mechanism were described to determine the parameters of key components. The main factors were then determined with the seed-filling performance of the seeding mechanism, according to the shape, number, installation inclination, and speed of the typed hole on the friction belt, as well as the thickness of the seed stem layer. The discrete element method (DEM) was used to establish a simulation model of the "seed stem group-hole friction zone". The single-factor simulation was realized to clarify the influence of each factor on the seed-filling performance. The optimal combination of factors and parameters was determined after quadratic regression and orthogonal rotational simulation. Taking the installation inclination angle of the seeding hole friction zone, the thickness of the seeding stem layer, and the speed of the seeding hole friction zone as the factors, the mathematical regression model of each factor was established for the evaluation index. The results showed that the influencing factors of the seed filling index and the leakage index were ranked in descending order of the speed of the type hole friction belt, the installation angle of the type hole friction belt, and the thickness of the seed stem layer. The optimal combination of factors and parameters was achieved in the bench test. The seed filling qualification index was 94.13% for the precut cassava-type friction belt precision seeding mechanism, and the leakage index was 3.77% when the thickness of the seed stem layer was rounded to 220-280 mm, the speed of the friction belt was 0.6 m/s, the installation inclination of the friction belt was 45°, the shape of the friction belt was C-shaped and the number of holes was 12. A better performance was achieved, where the seed filling index and the leakage index were 94.13% and 3.77%, respectively. The finding can provide a theoretical reference for the development of cassava precision seeders. [ABSTRACT FROM AUTHOR]

Corydalis saxicola is a perennial herbaceous plant, which mainly distributed in the karst areas of southwest China. Due to its important ecological and medicinal value, Corydalis saxicola has great prospects for development and utilization. With fragile living environmental condition and anthropogenic harvesting pressure, the wild resource of Corydalis saxicola is on the verge of exhaustion, so it has been included in the list of national key protected wild plants in 2021. In order to explore the causes for the endangerment of Corydalis saxicola and provide reference for its conservation and seedling breeding, this paper investigated the influencing factors on seed germination that including seed preservation methods, chemical pre-treatments, temperature, light, drought, pH, mix with wet sand and refrigerate treatment. The results were as follows: (1) Low-temperature storage can prolonged the seed activity, germination rate of 2-years stored seed could still reached 30%. (2) Germination rates of seed pre-treatments by HCl and NaClO solutions can reached to 50%～60%; germination rate was 50% at 20℃, while closed to zero at 30℃; darkness was more favorable for seed germination than periodic light condition; the seed germination rate kept decreasing with deepening drought; the seed germination rate did not changed significantly under the conditions of pH 3.0～8.0. (3) Mix with wet sand and refrigerate treatment can significantly promoted the seed germination potential and germination rate, and the germination rate was twofold of the control. In summary, the seed inactivation at room temperature and germination inability at high temperatures above 30℃ are more related to its endangerment; efficient seed germination methods can provide an effective way for conservation and industrial application of Corydalis saxicola. The results provide a theoretical and technical guarantee for the conservation of wild Corydalis saxicola, and technically support is cultivation.

Corydalis saxicola is a perennial herbaceous plant, which mainly distributed in the karst areas of southwest China. Due to its important ecological and medicinal value, Corydalis saxicola has great prospects for development and utilization. With fragile living environmental condition and anthropogenic harvesting pressure, the wild resource of Corydalis saxicola is on the verge of exhaustion, so it has been included in the list of national key protected wild plants in 2021. In order to explore the causes for the endangerment of Corydalis saxicola and provide reference for its conservation and seedling breeding, this paper investigated the influencing factors on seed germination that including seed preservation methods, chemical pre-treatments, temperature, light, drought, pH, mix with wet sand and refrigerate treatment. The results were as follows:(1)Low-temperature storage can prolonged the seed activity, germination rate of 2-years stored seed could still reached 30%.(2)Germination rates of seed pre-treatments by HCl and NaClO solutions can reached to 50%～60%; germination rate was 50% at 20 ℃, while closed to zero at 30 ℃; darkness was more favorable for seed germination than periodic light condition; the seed germination rate kept decreasing with deepening drought; the seed germination rate did not changed significantly under the conditions of pH 3.0～8.0.(3)Mix with wet sand and refrigerate treatment can significantly promoted the seed germination potential and germination rate, and the germination rate was twofold of the control. In summary, the seed inactivation at room temperature and germination inability at high temperatures above 30 ℃ are more related to its endangerment; efficient seed germination methods can provide an effective way for conservation and industrial application of Corydalis saxicola. The results provide a theoretical and technical guarantee for the conservation of wild Corydalis saxicola, and technically support is cultivation. [ABSTRACT FROM AUTHOR]

In order to study the effect of different CO2 air conditioning treatments on the quality of fresh lotus seeds postharvest storage, this study used 'Shilihe No.1' fresh lotus seeds as the test material, and under the conditions of 4 ° and 5% O2, different CO2 were used (4%, 8% and 12%). The fresh lotus was stored for 20 days under controlled atmosphere, and its appearance and nutritional quality during storage were measured. The results showed that 4% CO2 treatment could reduce the weight loss rate and browning degree of lotus seeds, but the nutrient consumption was more serious. After 20 days of storage, the starch content (98.01 mg/g) was 6.98 mg/g lower than the control group (104.99 mg/g) ; at the end of storage, browning degree, weight loss rate, reducing sugar of 8% CO2 treatment were 1.63, 8.54%, 0.76%, respectively, POD and PPO activities were 163.44 U/(min·g), 99.63 U/(min·g), which were significantly lower than the control group and other modified atmosphere treatment groups (P<0.05), while the starch content was 119.68 mg/g, which was significantly higher than that of the control and other atmospheres. 12% CO2 treatment may cause the lotus seeds to be damaged by too high concentration of CO2. After 20 days of storage, the a* value (-10.03) was higher than the control group (-12.68), and the b* value (24.95) was lower than the control group (28.03), and the browning phenomenon was more obvious than that of the control group. This researches showed that 5% O2 + 8% CO2 was the most suitable treatment for fresh lotus seeds storage. This treatment also showed a positive effect in reducing the nutrient consumption of lotus seeds and protecting the green of the epidermis, providing new ideas and references for the research and development of fresh lotus postharvest preservation technology. [ABSTRACT FROM AUTHOR]

Copyright of Bulletin of Botanical Research is the property of Bulletin of Botanical Research Editorial Department and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

In order to understand the changes of quality indexes and provide new insights for the scientific storage and the predictionof quality change of high oil soybean during storage. In the present study, a biochemical investigation was carried out to determine the quality indicators of high-oil soybeans during seed storage. Two kinds of high-oil soybeans were collected from the Henan and Inner Mongolia of China. Different moisture (13.00%±0.20% and 15.00%±0.20%) were then adjusted and stored the seeds at 25℃ and 35℃ for 180 days. The quality indexes of high-oil soybeans were determined and analyzed every 30 days during storage. Some parameters were evaluated, including the malondialdehyde (MDA) content, electrical conductivity, and enzyme activities of catalase (CAT), as well as the peroxidase (POD) and polyphenol oxidase (PPO). A correlation analysis was also carried out between the physiological and biochemical indexes. A kinetic model was selected for the change trends analysis of electrical conductivity and MDA. The results showed that the activities of CAT, POD, and PPO decreased with the increase in storage time, whereas the electrical conductivity and MDA content increased significantly in the two kinds of high-oil soybeans. And the change range of them increased with the increasing of the initial moisture content and the storage temperature. The correlation analysis showed that the storage time presented a significant or extremely significant correlation with the physical and chemical indexes of the two high-oil soybeans. The correlation coefficients between the storage time with the MDA and PPO were 0.82 and -0.90, 0.81 and -0.92, in the Henan and Inner Mongolia soybeans, respectively. The initial water content was significantly negatively correlated with the CAT activity of the two soybeans. The correlation coefficients were -0.63 and -0.74, respectively, indicating no strong correlation with the indicators. At the same time, there was a positive correlation between the storage temperature and the electrical conductivity in the two soybeans, where the correlation coefficients were 0.66 and 0.61, respectively. Furthermore, the kinetic analysis showed that the zero-order kinetic model better fitted for both electrical conductivity and MDA of the two high-oil soybeans at different storage temperatures. The two indexes were attributed to the energy demand reaction during storage (ΔG>0). The zero-order kinetic response coefficient also increased with the increase of initial moisture content and storage temperature of soybeans. Moreover, there was a higher content of crude fat in the zero-order kinetic activation energy of MDA and the electrical conductivity in the Henan soybean, compared with the Inner Mongolia one. Consequently, the higher temperature, initial moisture content, and crude fat content accelerated the electrical conductivity and MDA during storage, leading to the faster quality deterioration of the high-oil soybean. Therefore, a strict control of the temperature and initial moisture content during storage can be expected to maintain the fat content of seeds for the less quality deterioration of high-oil soybean. In particular with the high initial moisture content, the temperature conditions should be strictly regulated for the storage safety of the soybeans. Meanwhile, MDA content of high-oil soybean was more likely to change during storage than other indexes, which could be used as an early reference index for the quality change prediction. [ABSTRACT FROM AUTHOR]

Egg storage for fertilization and seed production has great significance in aquaculture. Giant grouper(Epinephelus lanceolatus)is an economically important aquaculture species in China, and its fertilized eggs are much expensive. In this study, we tried to develop an in vitro egg storage system in this species. Storage conditions such as medium, temperature and pH were experimentally investigated. Results showed that eggs stored in L-15 medium(pH=8. 1)at 22 C for 2 h showed a relatively high fertilization rate(66. 4%) and a hatching rate(47. 9%). With supplementation of giant grouper serum and vitamin premix into L-15 medium, the storage time was prolonged and eggs still retained a relatively high viability. The fertilization and hatching rates from eggs stored for 8 h were 64. 4% and 44. 8%, respectively. This optimized short-term egg storage system would facilitate breeding practice and hatchery management in giant grouper. [ABSTRACT FROM AUTHOR]

【Objective】To improve seed viability and eliminate the bottleneck preventing wine sorghum planting and industrial development in Guizhou Province.【Methods】The germination ability of wine sorghum seeds was studied using variety Hongyingzi as test material and polyethylene glycol (PEG)as priming agent, under different priming time (12, 24, 36, 48 h), redrying degree (7%, 10%, 15%), storage time (0, 30, 60, 90 d), and storage temperature (constant temperature at 25℃ or room temperature).【Results】 Any one of the element among priming time, redrying degree, or storage period all had significant impact on seed germination ability (P<0.05). There were interactions between priming time and redrying degree, priming time and storage temperature, or priming time, redrying degree and storage temperature. Wine sorghum seed had significant higher (P<0.05)germination rate than other treatments after 12 hours priming and 7% redrying, 24 hours priming and 10% redrying, or 48 hours priming and 7% redrying. Seed germination uniformity was high after 12 hours priming and 7% redrying, or 36 hours priming and 15% drying, at 25℃ constant temperature or room temperature.【Conclusion】The optimal conditions for wine sorghum seeds germination are 12 hours priming and 7% drying or 36 hours priming and 15% drying. The seeds could be well stored at 25℃ constant temperature or at room temperature. [ABSTRACT FROM AUTHOR]

Copyright of Shipin Kexue/ Food Science is the property of Food Science Editorial Department and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Food Research & Development is the property of Food Research & Development Editorial Department and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Desiccation method has been successfully applied for the cryopreservation of Bletilla formosana seeds (Orchidaceae). This study investigated the effects of cryostorage duration on the viability of B. formosana and Phalaenopsis aphrodite subsp. formosana seeds after cryopreservation. After 24 h desiccation by silica gel and cryopreservation for 48 mo, B. formosana seeds still remained high germination percentage (87.7%) and higher than fresh seeds. Phal. aphrodite subsp. formosana seeds could retained significant higher germination percentage (100.0%) than fresh seed treatment (74.6%) after cryopreservation for 15 mo. The plantlet from cryostoraged seeds could grow and develop well. The germination percentage of dried and cryopreserved seed for 24 h was higher than that of fresh seeds after cryopreservation. Under scanning electron microscopy, it is clear that immersing seeds in liquid nitrogen (LN) induces breakage in the seed coat, then cracks in the seed coat provide channels that facilitate hydration and may increase germination. The technique could be applied for the pre-treatment prior to cryopreservation of Phaius tankervilleae polllen, Phal. aphrodite subsp. formosana, Vanda lamellata and Vanda denisoniana seeds. The results showed that the germination percentage of freshly harvested pollen of P. tankervilleae was 81.4%, pollens directly placed in LN was low (25.4%) after thawing. However, the germination percentage increased to 81.0% for seeds dried with silica gel for 24 h prior to cryopreservation. In the case of seed preservation, the water content of V. lamellata fresh seeds was 34.6%, then decreased to 4.2% after desiccation for 24 h. The germination percentage of Phal. aphrodite subsp. formosana, V. lamellata and V. denisoniana fresh seeds were 77.5, 93.8 and 100.0%, respectively. The germination percentage of seeds that were directly placed in LN was low (0.0, 0.0 and 1.6%) after thawing, instead, the percentage increased to 88.0, 100.0 and 100.0% for seeds dried with silica gel for 24 h prior to cryopreservation for 24 h, respectively. These findings demonstrate that the seeds of V. lamellata exhibit a high tolerance toward desiccation and is suitable to be preserved at low temperature for long-term, and can be considered as an orthodox seed storage. Therefore, silica gel desiccation could be also applied for P. tankervilleae pollens, Phal. aphrodite subsp. formosana seeds, V. lamellata seeds and V. denisoniana seeds prior to cryopreservation. [ABSTRACT FROM AUTHOR]

Copyright of Chinese Journal of Oil Crop Sciences is the property of Oil Crops Research Institute of Chinese Academy of Agricultural Sciences and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Forest Research is the property of Forest Research Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Ｗｅ ｓｔｕｄｉｅｄ ｔｈｅ ｄｅｈｙｄｒａｔｉｏｎ ｓｅｎｓｉｔｉｖｉｔｙ ａｎｄ ｓｔｏｒａｇｅ ｃｈａｒａｃｔｅｒｉｓｔｉｃｓ ｏｆ Ｇ. ｐａｕｃｉｎｅｒｖｉｓ ｓｅｅｄｓ ｂｙ ｍｅａｓｕｒｉｎｇ ｔｈｅ ｇｅｒｍｉｎａｔｉｏｎ ｏｆ ｓｅｅｄｓ ｗｉｔｈ ｄｉｆｆｅｒｅｎｔ ｄｅｈｙｄｒａｔｉｏｎ ｅｘｔｅｎｔｓ ａｎｄ ｔｈｅ ｗａｔｅｒ ａｂｓｏｒｐｔｉｏｎ ａｆｔｅｒ ｒｅｈｙｄｒａｔｉｏｎ, ｔｈｅ ｃｈａｎｇｅ ｏｆ ｐｈｙｓｉｏｌｏｇｉｃａｌ ｉｎｄｉｃｅｓ ｏｆ ｒｅｓｉｓｔａｎｃｅ ｉｎ ｔｈｅ ｐｒｏｃｅｓｓ ｏｆ ｄｅｈｙｄｒａｔｉｏｎ ａｓ ｗｅｌｌ ａｓ ｔｈｅ ｓｅｅｄ ｇｅｒｍｉｎａｔｉｏｎ ｕｎｄｅｒ ｄｉｆｆｅｒｅｎｔ ｓｔｏｒａｇｅ ｍｅｔｈｏｄｓ. Ｔｈｅ ｒｅｓｕｌｔｓ ｗｅｒｅ ａｓ ｆｏｌｌｏｗｓ: (１) Ｔｈｅ ｉｎｉｔｉａｌ ｍｏｉｓｔｕｒｅ ｃｏｎｔｅｎｔ ｏｆ Ｇ. ｐａｕｃｉｎｅｒｖｉｓ ｓｅｅｄｓ ｗａｓ ４５.２９％. Ｔｈｅ ｄｅｈｙｄｒａｔｉｏｎ ｒａｔｅ ｗａｓ ｕｐ ｔｏ ４５％ ｗｈｅｎ ｐｌａｃｅｄ ａｆｔｅｒ ３５ ｄ ｉｎ ｔｈｅ ｉｎｄｏｏｒ ｖｅｎｔｉｌａｔｅｄ ｐｌａｃｅ. (２) Ｗｈｅｎ ｔｈｅ ｄｅｈｙｄｒａｔｉｏｎ ｒａｔｅ ｗａｓ ｌｅｓｓ ｔｈａｎ １８％, ｇｅｒｍｉｎａｔｉｏｎ ｐｅｒｃｅｎｔａｇｅ(ＧＰ) ａｎｄ ｔｈｅ ｗａｔｅｒ ａｂｓｏｒｐｔｉｏｎ ｒａｔｅ ａｆｔｅｒ ｒｅｗａｔｅｒｉｎｇ ｗｅｒｅ ｎｏｔ ｓｉｇｎｉｆｉｃａｎｔｌｙ ｄｉｆｆｅｒｅｎｔ ｆｒｏｍ ｔｈｏｓｅ ｏｆ ｆｒｅｓｈ ｓｅｅｄｓ. Ｈｏｗｅｖｅｒ, ｗｈｅｎ ｔｈｅ ｄｅｈｙｄｒａｔｉｏｎ ｒａｔｅ ｅｘｃｅｅｄｅｄ １８％, ＧＰ ａｎｄ ｔｈｅ ｗａｔｅｒ ａｂｓｏｒｐｔｉｏｎ ｒａｔｅ ａｆｔｅｒ ｒｅｗａｔｅｒｉｎｇ ｗｅｒｅ ｓｉｇｎｉｆｉｃａｎｔｌｙ ｄｅｃｒｅａｓｅｄ, ａｎｄ ＧＰ ｗａｓ ０ ａｔ ｄｅｈｙｄｒａｔｉｏｎ ｒａｔｅ ４２％. Ｔｈｅ ｃｒｉｔｉｃａｌ ｍｏｉｓｔｕｒｅ ｃｏｎｔｅｎｔ ｏｆ ｓｅｅｄｓ ｗａｓ ２７.２９％, ａｎｄ ｔｈｅ ｌｅｔｈａｌ ｍｏｉｓｔｕｒｅ ｃｏｎｔｅｎｔ ｏｆ ５０％ ｗａｓ １２.７２％. (３) Ｔｈｅ ｒｅｌａｔｉｖｅ ｃｏｎｄｕｃｔｉｖｉｔｙ, ｔｈｅ ｃｏｎｔｅｎｔｓ ｏｆ ｓｏｌｕｂｌｅ ｓｕｇａｒ ａｎｄ ｐｒｏｌｉｎｅ ｗｅｒｅ ｉｎｃｒｅａｓｅｄ ｇｒａｄｕａｌｌｙ ｗｉｔｈ ｔｈｅ ｄｅｅｐｅｎｉｎｇ ｏｆ ｓｅｅｄ ｄｅｈｙｄｒａｔｉｏｎ. Ｔｈｅ ｃｏｎｔｅｎｔ ｏｆ ｍａｌｏｎｄｉａｌｄｅｈｙｄｅ (ＭＤＡ) ｃｈａｎｇｅｄ ｌｉｔｔｌｅ ｗｈｅｎ ｔｈｅ ｄｅｈｙｄｒａｔｉｏｎ ｒａｔｅ ｗａｓ ｌｅｓｓ ｔｈａｎ ２４％, ａｎｄ ｉｎｃｒｅａｓｅｄ ｓｉｇｎｉｆｉｃａｎｔｌｙ ｗｈｅｎ ｅｘｃｅｅｄｉｎｇ ２４％. Ｂｏｔｈ ａｃｔｉｖｉｔｉｅｓ ｏｆ ｓｕｐｅｒｏｘｉｄｅ ｄｉｓｍｕｔａｓｅ (ＳＯＤ) ａｎｄ ｐｅｒｏｘｉｄａｓｅ (ＰＯＤ) ｓｈｏｗｅｄ ａ ｔｒｅｎｄ ｏｆ ｆｌｕｃｔｕａｔｉｏｎ ａｎｄ ｐｅａｋｅｄ ａｔ ｄｅｈｙｄｒａｔｉｏｎ ｒａｔｅ １８％. (４) Ｓｅｅｄｓ ｗｉｔｈ ｄｅｓｉｃｃａｔｉｏｎ ｓｔｏｒａｇｅ ｆｏｒ ｏｎｅ ｍｏｎｔｈ ａｔ ｒｏｏｍ ｔｅｍｐｅｒａｔｕｒｅ, ａｎｄ ａｌｌ ｔｈｅ ｓｅｅｄｓ ｗｉｔｈ ｓｔｏｒａｇｅ ｉｎ ｄａｍｐ ｓａｎｄ ａｔ －１ ℃ ａｎｄ －２０ ℃ ｆｏｒ ｏｎｅ ｍｏｎｔｈ ｃｏｕｌｄ ｎｏｔ ｇｅｒｍｉｎａｔｅ. ＧＰ ｏｆ ｓｅｅｄｓ ｗｉｔｈ ｗａｔｅｒ ｉｍｍｅｒｓｉｏｎ ｆｏｒ ｏｎｅ ｍｏｎｔｈ ｄｒｏｐｐｅｄ ｓｉｇｎｉｆｉｃａｎｔｌｙ. Ｇｅｒｍｉｎａｔｉｏｎ ｐｒｏｃｅｓｓｅｓ ｏｆ ｓｅｅｄｓ ｗｉｔｈ ｓｔｏｒａｇｅ ｉｎ ｄａｍｐ ｓａｎｄ ｆｏｒ ｏｎｅ ｍｏｎｔｈ, ｔｈｒｅｅ ｍｏｎｔｈｓ ｏｒ ｓｉｘ ｍｏｎｔｈｓ ａｔ ４ ℃ ｗｅｒｅ ｄｅｌａｙｅｄ, ｂｕｔ ｔｈｅｒｅ ｗａｓ ｎｏ ｓｉｇｎｉｆｉｃａｎｔ ｅｆｆｅｃｔ ｏｎ ＧＰ. Ｉｔ ｉｎｄｉｃａｔｅｓ ｔｈａｔ ｗｈｅｎ ｔｈｅ ｄｅｈｙｄｒａｔｉｏｎ ｒａｔｅ ｏｆ Ｇ. ｐａｕｃｉｎｅｒｖｉｓ ｉｓ ｌｅｓｓ ｔｈａｎ １８％, ｓｅｅｄｓ ｃａｎ ｒｅｇｕｌａｔｅ ｒｅｓｉｓｔａｎｃｅ ｔｏ ｍａｉｎｔａｉｎ ｎｏｒｍａｌ ｍｅｔａｂｏｌｉｓｍ ｏｆ ｃｅｌｌｓ. Ｔｈｅ ｓｅｅｄｓ ｃａｎ ｅｎｄｕｒｅ ａ ｃｅｒｔａｉｎ ｄｅｇｒｅｅ ｏｆ ｄｅｈｙｄｒａｔｉｏｎ ａｎｄ ｌｏｗ ｔｅｍｐｅｒａｔｕｒｅ. Ｗｈｅｎ ｔｈｅ ｄｅｈｙｄｒａｔｉｏｎ ｒａｔｅ ｅｘｃｅｅｄｓ １８％, ｔｈｅ ｍｅｔａｂｏｌｉｓｍ ｏｆ ｓｅｅｄｓ ｉｓ ｕｎｂａｌａｎｃｅｄ, ａｎｄ ｔｈｅｎ ｄｅｔｅｒｉｏｒａｔｅｄ ｕｎｔｉｌ ｄｅａｔｈ, ｗｈｉｃｈ ｂｅｌｏｎｇ ｔｏ ｍｉｎｉｍａｌｌｙ ｒｅｃａｌｃｉｔｒａｎｔ ｓｅｅｄｓ. Ｉｔ ａｌｓｏ ｉｎｄｉｃａｔｅｓ ｔｈａｔ ｓｔｏｒａｇｅ ｉｎ ｄａｍｐ ｓａｎｄ ａｔ ４ ℃ (ｍｏｉｓｔｕｒｅ ｃｏｎｔｅｎｔ ７.５％) ｉｓ ａ ｇｏｏｄ ｍｅｔｈｏｄ ｆｏｒ ｓｈｏｒｔ￣ｔｅｒｍ ｓｔｏｒａｇｅ ｏｆ Ｇ. ｐａｕｃｉｎｅｒｖｉｓ ｓｅｅｄｓ. [ABSTRACT FROM AUTHOR]

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