[Objective] To solve the problems of low efficiency, small coverage, and inability to trace back in real- time in the supervision of biosafety laboratories, Zhejiang Province constructed a biosafety supervision matrix of 'one platform + four systems' to achieve fine and intelligent supervision of biosafety laboratories. [Methods]A new biosafety laboratory monitoring system has been built to receive real-time data on laboratory personnel, equipment, highly pathogenic biological samples, experimental behavior, the environment, and other biosafety elements and to achieve encrypted storage, analysis, early warning, and transmission over a dedicated network and authorized query functions. The new laboratory biosafety evaluation system ensures full traceability of laboratory inspection and evaluation and establishes a closed-loop assessment of self-inspection, mutual inspection, report, rectification, and re-inspection to urge laboratories to rectify the problems promptly. The new pathogenic microorganism sample transport system integrates the Internet of Things, wireless transmission, BeiDou positioning, remote control, geographic information system maps, and other technologies on the intelligent sample transport box to achieve sample transport path monitoring, track positioning, data synchronization, abnormal alarm, and other online transport monitoring functions. Whole-process safety protection of pathogenic microorganism strains (viruses) and the sample transport process, which is visible, traceable, and preventable, is realized. Docking with the pathogenic microorganism laboratory filing system is implemented to achieve real-time supervision of laboratory personnel, equipment, and subject filing. A new biosafety online monitoring platform is proposed to visually present the "unit--county (district)--city" overall biosafety situation, including sample transportation (e.g., status monitoring, receiving units, and approval units), laboratory safety (e.g., self-examination issues, problems spotted during laboratory assessment, and problems that have not been rectified), laboratory filing for cities and counties (districts), and visual evaluation of experimental activities. To solve the problems of low efficiency, small coverage, and high risk of biosafety laboratory supervision system, Zhejiang Province has built a biosafety supervision matrix of 'one platform + four systems' to realize the fine and intelligent supervision of biosafety laboratories. Based on the Java EE 11 technical specification, the RESTful specification is implemented on the traditional MVC architecture model, and the 'biosafety online' supervision platform, 'Sheng'an Eye' supervision system, laboratory biosafety evaluation system, pathogenic microorganism sample transportation system, and pathogenic microorganism laboratory filing system are unified. [Results]The intelligent supervision system for biosafety laboratories has been deployed and used in more than 800 units in Zhejiang Province, recording approximately 200 000 pieces of abnormal data, helping the relevant laboratories to rectify more than 110 000 points of risk, and realizing early warning within 3 s of the environmental monitoring abnormalities and an accuracy of more than 95% in identifying abnormal behavior of personnel. The system helps laboratory managers discover and dispose of abnormal situations in time and realizes closed-loop management of laboratory biosafety. [Conclusions]The intelligent supervision system for biosafety laboratories achieves online supervision and closed-loop processing of laboratory biosafety, improves laboratory biosafety risk control and early warning and disposal capabilities, and strongly supports the modernization, intelligence, and refinement of the governance of biological laboratories. In the future, the linkage between the monitoring platform and other systems should be further expanded, the accuracy of algorithms, such as behavioral recognition, should be improved, and network channels and encryption algorithms should be strengthened. [ABSTRACT FROM AUTHOR]