Chernozem with pH 5.38, 6.54, 7.16 and 8.11 (No. A, B, C and D) were selected as the research object. The effects of nitrogen fertilizer (urea, diammonium phosphate) addition on the acidity change and acid-base buffing performance of chernozem were studied by constant temperature culture test. The results showed that urea and diammonium phosphate reduced the pH values of the four chernozems by 0.72, 0.53, 0.94, 0.44 and 0.89, 0.58, 1.18 and 0.54 units, respectively. For the four soils, the degree of pH reduction was in the order of C>B≥A>D. After the application of nitrogen fertilizer, the pH of sample D was still alkaline, so no exchangeable acid, H+ or Al3+ were detected. The exchangeable acid content of soil samples A, B and C increased by 0.07, 0.94, 3.62 times, 0.23, 0.74, 2.93 times, exchangeable H+ by 0.12, 1.14, 7.27 times, 0.26, 0.97, 4.73 times, exchangeable Al3+ by 0.15, 0.77, 2.35 times, 0.21, 0.54, 2.29 times, respectively. This indicated that the increase of exchangeable acid, H+ and Al3+ in chernozem after the addition of nitrogen fertilizer followed the order of C >B>A. As considering the addition of urea and diammonium phosphate, the contents of exchangable acid, H+ and Al3+ in chernozemic showed no significant difference. The contents of exchangeable acid, H+ and Al3+ of chernozem were increased with the decrease of soil pH value in the neutral and acidic range. After the addition of urea and diammonium phosphate, the acid-base buffer capacities of the four soil samples were decreased by 2.82%, 10.27%, 3.24%, 2.99% and 2.26%, 3.80%, 3.70% and 3.94%, respectively, and there was no significant difference between the addition of urea and diammonium phosphate. The acid-base buffer capacity of chernozem was significantly positively correlated with soil pH and organic matter content and negatively correlated with soil exchangeable H+ and AI3+. In conclusion, the addition of nitrogen fertilizer promoted the contents of exchangeable acid, H+ and Al3+ of chernozem to different degrees, and reduced the soil pH value and acid-buffer capacity. [ABSTRACT FROM AUTHOR]