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Objective To explore the correlation between the expression level of interferon gamma (IFN-γ) and the severity of patients with septic shock and its prognostic value. Methods The clinical data and serum of 96 septic shock patients admitted to the Department of Critical Care Medicine, the First Affiliated Hospital of Guangxi Medical University from March 2022 to August 2023 were collected, and divided into survival group and death group according to the 28-day outcome,collected at the same time Sera from 30 healthy people undergoing physical examination during the same period served as the control group. The enzyme-linked immunosorbent (ELISA) method was used to detect the expression levels of IFN- γ in the three groups, and the expression levels of IFN- γ and various clinical data between the groups were analyzed. Results The serum IFN- γ expression level of patients with septic shock was lower than that of healthy people undergoing physical examination, 212.80 (151.11, 255.79) ng/L compared with 343.37 (314.5, 427.95) ng/L, P < 0.01. Among the 96 cases of septic shock, There were 54 cases in the survival group and 42 cases in the death group. The clinical data of the two groups were compared. The gender, smoking history, SBP, DBP, SI, Lac, IL-6, IFN- γ, PCT, SOFA score, and APACHEII score of the two groups of patients were compared. The difference is statistically significant (P < 0.01). Logistic regression analysis showed that decreased expression of Lac, IFN-γ, and APACHEII score were independent risk factors for death in patients with septic shock. Odds ratio (OR) and 95% confidence interval (95%CI) were 6.491 (1.404 -30.004) respectively. 0.954 (0.954 - 0.999), 3.476 (1.210 - 9.984), P < 0.05. The Spearman correlation analysis of INF- γ, Lac and APACHE showed a negative correlation, and the correlation coefficients were -0.38 and -0.35 respectively. Drawing the ROC curves of Lac, IFN- γ, and APACHE, the AUCs of the three were 0.847, 0.869, and 0.833 respectively. The AUC of the three joint predictions was 0.978. The joint prediction value of the three was better than that of a single indicator, P < 0.001. Conclusion The decrease in IFN-γ expression level and the severity of septic shock patients have good prognostic value. [ABSTRACT FROM AUTHOR]

Copyright of Shipin Kexue/ Food Science is the property of Food Science Editorial Department and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

OBJECTIVE: Allergic rhinitis is a globally prevalent allergic disease that seriously affects people's quality of life and often has a poor clinical treatment effect. The effects of mesenchymal stem cells on allergic rhinitis animal models were systematically evaluated, hoping to provide inspiration for clinicians. METHODS: Articles related to the treatment of allergic rhinitis mice with mesenchymal stem cells were retrieved from CNKI, Wanfang, VIP, Chinese biomedical literature database, Embase, Cochrane Library, PubMed, and Web of Science. The retrieval time was from the establishment of the database to March 1, 2022. Data were independently extracted by two researchers. SYRCLE Animal Experiment Bias Risk Assessment table was used to evaluate the literature quality. Stata 16.0 was used for meta-analysis. RESULTS: A total of 152 mice were included in 6 randomized controlled animal experiments, including 51 mice in stem cell group, 51 mice in blank control group, and 50 mice in animal model control group. The meta-analysis results demonstrated that the number of sneezes in stem cell group was significantly less than that in the animal model control group [SMD=-9.83, 95%CI(-17.45,-2.22), P=0.011]. The number of nose rubbing in stem cell group was significantly less than that in the animal model control group [SMD=-13.27, 95%CI(-25.04,-1.49), P=0.027]. Serum interleukin-4 level in stem cell group was significantly smaller than that in the animal model control group [SMD=-8.23, 95%CI(-11.28, -5.17), P=0]. Serum interleukin-6 level in stem cell group was significantly smaller than that in the animal model control group [SMD=-4.27, 95%CI(-5.43,-3.10), P=0.035]. Interleukin-10 level in the spleen in stem cell group was significantly higher than that in the animal model control group [SMD=25.63, 95%CI(17.92, 33.33), P=0]. Interleukin-6 level in the spleen in stem cell group was significantly lower than that in the animal model control group [SMD=-40.64, 95%CI(-80.16, -1.13), P=0]. CONCLUSION: According to the included animal experiments, stem cells help to improve the symptoms of allergic rhinitis, decrease serum interleukin-4 and interleukin-6 levels and spleen interleukin-6 level, and increase spleen levels of interleukin-10. Stem cells play an important role in allergic rhinitis, and clinical trials are expected to carefully evaluate their safety and efficacy in the future. [ABSTRACT FROM AUTHOR]

[Objectives]This study was conducted to investigate the effects of xylo-oligosaccharides(XOS)and coated sodium butyrate(CSB)on intestinal development, mucosal immunity and cecal microorganism in broilers. [Methods]The 2×2 factorial test design was adopted, with two levels of XOS(0 and 150 mg·kg-1)and two levels of CSB(0 and 400 mg·kg-1). A total of 192 one-day-old Ross 308 broiler were randomly divided into 4 groups with 6 replicates per group and 8 broilers per replicate.There were Con(Control, basal diet), XOS(150 mg·kg-1XOS), CSB(400 mg·kg-1CSB)and XOS+CSB(150 mg·kg-1XOS+400 mg·kg-1CSB)groups, respectively. The trial lasted for 42 d. [Results]CSB supplementation decreased the relative length of the duodenum and ileum(P<0.05)and increased the weight per unit length of the small intestine(P<0.05). An interaction effect between XOS and CSB was observed for ileum weight per unit length(P<0.05). XOS supplementation increased the number of intraepithelial lymphocytes(IEL)in the jejunum and ileum and IgA-producing(IgA+)cells in the jejunum(P<0.05). At the same time, CSB supplementation also increased the number of IEL in the small intestine and the number of IgA+cells in the duodenum and ileum, and a significant interaction was observed for the number of IEL in the ileum(P<0.05). Moreover, XOS supplementation reduced the expression of interferon gamma(IFN-γ)and toll-like receptor(TLR2)in the jejunum and ileum(P<0.05). CSB supplementation showed lower level of interleukin-1β(IL-1β)mRNA expression in the jejunum(P<0.05), and IL-1β and IFN-γ mRNA expression in the ileum(P<0.05). Simultaneously, a significant interaction was found for the ileal expression of IL-1β(P<0.05). Additionally, XOS supplementation increased the number of Bifidobacteria(P<0.05)and CSB supplementation tended to increase Bifidobacteria number(0.05

OBJECTIVE: To explore the effect of Zhuang medicine tincture combined with finger acupuncture on nerve function and inflammatory factors after hypertensive intracerebral hemorrhage. METHODS: A total of 100 postoperative hypertensive intracerebral hemorrhage patients who were hospitalized in Wuming District Traditional Chinese Medicine Hospital of Nanning City from March 2017 to March 2020 were included as the research objects. According to the random number table method, they were divided into observation group and control group. Group 50 cases. The patients in the control group were treated with conventional rehabilitation, and the patients in the observation group were treated with Zhuang medicine tincture combined with finger acupuncture on the basis of conventional rehabilitation, and both were treated for 6 weeks. To observe the clinical efficacy of the two groups of patients, the National Institutes of Health Stroke Scale (NIHSS) scores and the Fugl-Meyer Motor Function Scale (Fugl-Meyer) scores after 1 month and 3 months of treatment Assessment, FMA) score, serum interleukin 1β (IL-1β), interferon gamma (IFN-γ) and interleukin 10 (IL-10) levels; Glasgow outcome scale (Glasgow outcome) after 3 months of treatment scale, GOS) score. RESULTS: The total effective rate of patients in the observation group was 90.00% (45/50), which was significantly higher than 74.00% (37/50) in the control group. The difference was statistically significant (P <0.05). Compared with the control group, the NIHSS score of the observation group decreased more significantly after 1 month and 3 months of treatment, and the FMA score increased more significantly, and the differences were statistically significant (P<0.05). The GOS score of the observation group after 3 months of treatment was significantly higher than that of the control group, and the difference was statistically significant (P<0.05). Compared with the control group, the levels of IL-1β, IFN-γ and IL-10 in the observation group decreased more significantly after 1 month and 3 months of treatment, and the differences were statistically significant (P<0.05). CONCLUSION: Zhuang medicine tincture combined with finger acupuncture can improve the nerve function of patients after hypertensive cerebral hemorrhage and reduce the level of inflammatory factors, which can be further verified by large-sample, multi-center clinical research. [ABSTRACT FROM AUTHOR]

Objective: To study the effects of Jiedu Quyou Decoction combined with local interferon blocking on the serum levels of Interferon-γ (IFN-γ) and Transforming growth factor-β (TGF-β) in patients with multiple plantar warts Impact. Methods: 81 patients with multiple plantar warts who were diagnosed and treated in our hospital from January 2016 to December 2019 were selected and randomly divided into two groups. The control group was treated with interferon local blocking treatment, and the observation group was treated with Jiedu Quyou Decoction combined with interferon local blocking treatment. Serum levels of IFN-γ, TGF-β and T lymphocyte subsets were detected in the two groups, and adverse reactions such as fatigue, fever, nausea, headache and leukopenia were recorded in patients with multiple plantar warts. Results: The effective rate of patients with multiple plantar warts in the observation group was 95.00%, which was significantly higher than that in the control group (70.32%, P<0.05); after treatment, the serum IFN-γ levels of patients with multiple plantar warts in the two groups were significantly increased (P <0.05), serum TGF-β levels were significantly reduced (P<0.05), and serum IFN-γ and TGF-β levels in the observation group were significantly better than those in the control group (P<0.05); after treatment, the two groups had multiple plantar warts The patient's CD3 +, CD4 + and CD4 + /CD + levels were significantly increased (P<0.05), CD8 + levels were significantly reduced (P<0.05), and the observation group changed more significantly (P<0.05); two groups were more frequent There was no significant difference in the incidence of fatigue, fever, nausea, headache and leukopenia in patients with plantar warts (P>0.05). Conclusion: Jiedu Quyou Decoction combined with local interferon blocking can significantly reduce the serum IFN-γ and TGF-β levels in patients with multiple plantar warts, improve the curative effect, and improve the immune function. It is worthy of promotion. [ABSTRACT FROM AUTHOR]

Objective: To explore the effect and mechanism of Eltrombopag combined with azathioprine in treatment of refractory idiopathic thrombocytopenic purpura(RITP). Methods: 61 patients with refractory idiopathic thrombocytopenic purpura admitted to our hospital from January 2016 to January 2020 were selected as the research subjects.According to the order of admission, they were divided into two groups according to the random number table method. 30 patients in the control group were treated with azathioprine, and 31 patients in the study group were treated with eztropa combined with azathioprine.The treatment effects, peripheral blood Th lymphocyte factor levels, B1 lymphocyte percentage, and oxidative stress response indexes were compared between the two groups. Results: The total effective rate of treatment in the study group was 90.32 %, which was significantly higher than that in the control group(73.33 %, P <0.05). Before treatment, there was no significant difference in the levels of interleukin(IL)-2, interferon gamma(IFN-γ), IL-4, and IL-5 between the two groups of patients(P>0.05). After treatment, the levels of IL-2 and IFN-γ in the two groups decreased, and the levels of IL-4 and IL-5 increased, and the study group was better(P<0.05). Before treatment, there was no significant difference in CD19+lymphocyte percentage and CD5+CD19+lymphocyte percentage between the two groups(P>0.05). After treatment, the percentage of CD19+lymphocytes and CD5+CD19+lymphocytes in two groups were significantly lower, and the study group was significantly lower than the control group(P<0.05). Before treatment, there was no significant difference in the levels of total antioxidant capacity(TAC),superoxide dismutase(SOD), melatonin(MT) and glutathione(GSH) between the two groups(P>0.05). After treatment, the TAC, SOD,MT, and GSH levels of the two groups were significantly higher, and the study group was significantly higher than the control group(P<0.05). Conclusion: Eltrombopag combined with azathioprine has a significant effect on RITP, and its mechanism may be related to improving the immune function and oxidative stress state of the patient. This method is worthy of recommendation to be widely used in clinic. [ABSTRACT FROM AUTHOR]

Objective: To explore the effect and mechanism of Eltrombopag combined with azathioprine in treatment of refractory idiopathic thrombocytopenic purpura(RITP). Methods: 61 patients with refractory idiopathic thrombocytopenic purpura admitted to our hospital from January 2016 to January 2020 were selected as the research subjects.According to the order of admission, they were divided into two groups according to the random number table method. 30 patients in the control group were treated with azathioprine, and 31 patients in the study group were treated with eztropa combined with azathioprine.The treatment effects, peripheral blood Th lymphocyte factor levels, B1 lymphocyte percentage, and oxidative stress response indexes were compared between the two groups. Results: The total effective rate of treatment in the study group was 90.32 %, which was significantly higher than that in the control group(73.33 %, P <0.05). Before treatment, there was no significant difference in the levels of interleukin(IL)-2, interferon gamma(IFN-γ), IL-4, and IL-5 between the two groups of patients(P>0.05). After treatment, the levels of IL-2 and IFN-γ in the two groups decreased, and the levels of IL-4 and IL-5 increased, and the study group was better(P<0.05). Before treatment, there was no significant difference in CD19+lymphocyte percentage and CD5+CD19+lymphocyte percentage between the two groups(P>0.05). After treatment, the percentage of CD19+lymphocytes and CD5+CD19+lymphocytes in two groups were significantly lower, and the study group was significantly lower than the control group(P<0.05). Before treatment, there was no significant difference in the levels of total antioxidant capacity(TAC),superoxide dismutase(SOD), melatonin(MT) and glutathione(GSH) between the two groups(P>0.05). After treatment, the TAC, SOD,MT, and GSH levels of the two groups were significantly higher, and the study group was significantly higher than the control group(P<0.05). Conclusion: Eltrombopag combined with azathioprine has a significant effect on RITP, and its mechanism may be related to improving the immune function and oxidative stress state of the patient. This method is worthy of recommendation to be widely used in clinic. [ABSTRACT FROM AUTHOR]

Objective: To study the effect of Bailing Capsule combined with Roflumilast in the treatment of elderly bronchial asthma and its influence on immune function. Methods: From March 2018 to March 2019, 110 elderly patients with bronchial asthma admitted to the outpatient department of our hospital were selected and randomly divided into two groups, with 55 cases in each group. The control group took Roflumilast tablets orally, 500 mg each time, once a day. Patients in the observation group took Bailing Capsules, 5 capsules each time, 3 times a day. The clinical control rates of the two groups were compared, and the changes in the levels of inflammatory factors, neutrophils, fractional exhaled nitric oxide (FeNO) and immune function in the induced sputum of the two groups before and after treatment. Results: After treatment, the clinical control rate of the observation group was 70.91% (39/55), which was significantly higher than that of the control group [40.00% (22/55)] (P<0.05); CD4 +, CD4 + /CD8 in the two groups + And CD3 + were significantly higher than before treatment, and CD4 +, CD4 + /CD8 + and CD3 + in the observation groupSignificantly higher than the control group (P<0.05); the absolute value of neutrophils, the percentage of neutrophils, interleukin-4 (IL-4), FeNO, and interleukins in the induced sputum of the two groups of patients -17 (interleukin-17, IL-17) were significantly lower than before treatment (P<0.05), and the interferon-γ (Interferon gamma, IFN-γ) in induced sputum was significantly higher than before treatment (P<0.05) ), and the absolute value of neutrophils, percentage of neutrophils, IL-4, FeNO, and IL-17 in induced sputum in the observation group were significantly lower than those in the control group (P<0.05), and IFN-γ in induced sputum All were significantly higher than the control group (P<0.05). Conclusion: Bailing Capsule combined with Roflumilast has a significant effect on elderly patients with bronchial asthma. The mechanism may be related to inhibiting the activation of neutrophils, regulating the balance of T lymphocyte subsets and inhibiting the release of related inflammatory factors. [ABSTRACT FROM AUTHOR]

Objective To study the effect of genetic variation on the prognosis of children with Epstein-Barr virus (EBV)-positive hemophagocytic lymphohistiocytosis (HLH) and its association with cytokines. Methods A total of 81 EBV-positive HLH children who received the sequencing of related genes were enrolled. According to the results of gene detection, they were divided into a non-mutation group and a mutation group. According to the pattern of gene mutation, the mutation group was further divided into three subgroups: single heterozygous mutation (SHM), double heterozygous mutation (DHM), and homozygous or compound heterozygous mutation (H-CHM). The serum levels of cytokines were measured and their association with HLH gene mutations was analyzed. Results UNC13D gene mutation had the highest frequency (13/46, 28%). The STXBP2 c.575G>A(p.R192H) and UNC13D c.604C>A(p.L202M) mutations (likely pathogenic) were reported for the first time. The mutation group had a significantly higher level of tumor necrosis factor alpha (TNF-α) than the non-mutation group, while it had a significantly lower level of interferon gamma (IFN-γ) than the non-mutation group (P<0.05). The IL-4 level of the DHM subgroup was higher than that of the non-mutation group, while the IL-4 level of the H-CHM subgroup was lower than that of the DHM group (P<0.0083). The H-CHM subgroup had a significantly lower 1-year overall survival rate than the non-mutation group, the SHM subgroup, and the DHM subgroup (39%±15% vs 85%±6%/86%±7%/91%±9%, P=0.001). Conclusions There is a significant reduction in IFN-γ level in the mutation group. Children with homozygous or compound heterozygous mutation tend to have poorer prognosis, while other mutations do not have a significant impact on prognosis. [ABSTRACT FROM AUTHOR]

BACKGROUND: Mesenchymal stem cells possess strong immunomodulatory capacity, mainly involved in the proliferation, differentiation and functional status of immune cells, and the secretion of inflammatory factors. The immunomodulatory capacity of mesenchymal stem cells is regulated by inflammatory factors. As the type and level of inflammatory factors in the microenvironment change, the immune regulation function of mesenchymal stem cells also changes. OBJECTIVE: To review the research progress of inflammatory factor intervention in the immune regulation of mesenchymal stem cells. METHODS: A computer-based retrieval of PubMed, Elsevier and CNKI databases was performed. The keywords were “mesenchymal stem cells, immune regulation, plasticity, interferon gamma, transforming growth factor beta, interleukin-17, interleukin-35, prostaglandins E2” in Chinese and English, respectively. The articles concerning the effects of inflammatory factor intervention on the immune regulation of mesenchymal stem cells were included. RESULTS AND CONCLUSION: After intervention and pre-treatment of interferon-γ, transforming growth factor-β, interleukin-17, interleukin-35 and prostaglandin E2, the biological characteristics of mesenchymal stem cells have also changed. The interventions cannot only reshape the tissue microenvironment and affect the inflammatory response, but also reconstruct the immune balance, which further treats or alleviates the disease progression. Reasonable individualized and differentiated treatments will be performed at different disease stages according to inflammatory and immunological reaction of the disease. All of them are expected to further improve the therapeutic effect of mesenchymal stem cells on immuno-inflammatory diseases. [ABSTRACT FROM AUTHOR]

Objective: To study the clinical value of serum interferon-gamma (IFN-gamma), interleukin-1 beta (Il-1 beta) and tumor necrosis factor-alpha (TNF-alpha) levels in patients with pulmonary tuberculosis. Methods: 25 tuberculosis patients treated in Shunyi District Hospital of Beijing from January 2015 to December 2018 were selected as the tuberculosis group, and 25 healthy people who underwent physical examination in the hospital during the same period were selected as the control group. The serum levels of IFN-gamma, Il-1 beta and TNF-alpha in tuberculosis group and control group were detected and compared by ELISA. The serum levels of IFN-gamma, Il-1 beta and TNF-alpha in sputum negative group (n=14 cases) and sputum positive group(n=11 cases), non-cavity group (n=15 cases) and cavity group(n=10 cases) were compared. Results: The serum levels of IFN-gamma, Il-1 beta and TNF-alpha in tuberculosis group were significantly higher than those in control group (P < 0.05). The serum levels of IFN-gamma, Il-1 beta and TNF-alpha in sputum positive group were significantly higher than those in sputum negative group(P < 0.05). The serum levels of IFN-gamma, Il-1 beta and TNF-alpha in patients with pulmonary tuberculosis in the cavity group were significantly higher than those in the non-cavity group (P < 0.05). Conclusion: The serum levels of IFN-gamma, Il-1 beta and TNF-alpha in patients with pulmonary tuberculosis were significantly higher than those in healthy people. These cytokines may help to judge the course of disease and play an important role in the pathogenesis of tuberculosis. [ABSTRACT FROM AUTHOR]

Copyright of Chinese Journal of Contemporary Pediatrics is the property of Xiangya Medical Periodical Press and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Practical Pharmacy & Clinical Remedies is the property of Editorial Department of Practical Pharmacy & Clinical Remedies and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Journal of Modern Laboratory Medicine is the property of Journal of Modern Laboratory Medicine Editorial Department and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Objective: To study the effect of total glucosides of paeony on the serum tumor necrosis factor (TNF-α), Interferon-γ (IFN-γ) and interleukin-10 (IL-10) levels of patients with erosive oral lichen planus. Methods: 80 patients of erosive oral lichen planus who were treated from March 2014 to March 2015 in our hospital were selected as the research objects. The control group was treated with Low concentration tacrolimus, while the observation group was treated with Total glucosides of paeony, Then the Changes of IL-10, IFN- and TNF-α, erosion area, pain score before and after, the short-term therapeutic effect were compared. Results: After treatment, the serum TNF-α, IL-10 levels in the Observation group were lower than those of the control group, the seum IFN -γ level was higher than those of the control group[(2.16土 0.61)μg/mL vs(3.04土 0.80)μg/m L，（258.93土 5.72)ng/L vs(273.41 土 6.03)ng/L，（319.27土 53.46)ng/L vs(290.95土 51.03)ng/L(P< 0.05); the erosion area, pain score of the Observation group were less than those of the control group[(0.10土 0.03)cm2 vs(0.51 ± 0.10)cm2, (1.01土 0.30)score vs(3.20± 0.78)score](P< 0.05), the short-term effective rate of observation group was statistically higher than that of the control group [95.00%(38/40)vs75.00%(30/40)(P< 0.05). Conclusion: Total glucosides of paeony was well for the erosive oral lichen planus, which could regulate the serum TNF-α, IFN-γ and IL-10 level and had immunomodulatory effects. [ABSTRACT FROM AUTHOR]

Objective To investigate the effect of radiofrequency ablation on serum tumor necrosis factor alpha-α (TNF-α), interferon gamma-γ(IFN-γ) and interleukin 17 (IL-17) levels in patients with lumbar disc herniation. Methods We studied 276 patients with lumbar disc herniation admitted to the First Affiliated Hospital of Xi’an Jiaotong University from January 2010 to December 2015. The experimental group received radiofrequency thermocoagulation and target ablation decompression treatment. In the same period, 133 healthy subjects were selected as control group. We collected the general information of the admitted patients and their serum before and after operation as well as serum of the control group. The changes of TNF-α, IFN-γ and IL-17 levels were detected by ELISA method. Results The levels of serum TNF-α, IFN-γ and IL-17 in the patients with lumbar disc herniation were significantly higher than those in the control group (P<0.05). Compared with those before operation, the serum levels of TNF-α, IFN-γ and IL-17 were decreased significantly in the recovery phase after radiofrequency ablation (P<0.05). Conclusion Frequency thermal coagulation target ablation decompression treatment of lumbar disc herniation can not only improve pain symptoms but also reduce the systemic inflammatory reactions. Therefore, it deserves clinical application. [ABSTRACT FROM AUTHOR]

Objective To observe the effect of plasma exchange (PE) therapy for removing serum interleukin - 6 (IL - 6), tumor necrosis factor alpha (TNFɑ), and interferon gamma (IFNɣ) in patients with liver failure, and to assess the value of artificial liver support system in the treatment of liver failure. Methods The study included 36 patients with liver failure who were hospitalized in the First Affiliated Hospital of Bengbu Medical College from February 2012 to February 2013. Blood samples were collected before PE and the next morning after multiple treatments. Plasma TNFɑ, IL - 6, and IFNɣ levels were measured by ELISA. Between - group comparison of measurement data before and after treatment was made by independent - samples t test, and comparison of mean values between groups was made by analysis of variance. Results Al the 36 liver failure patients had significantly lower levels of TNFɑ (381.23 ± 190.57 ng/L vs 274.12 ± 212.30 ng/L, P < 0.05), IL - 6 (77.9 ± 83.09 ng/L vs 54.8 ± 63.32 ng/L, P < 0.05), and IFNɣ (534.65 ± 471.19 ng/L vs 259.65 ± 312.26 ng/L, P < 0.05) after treatment than before treatment. According to the severity of clinical manifestations, the patients with subacute or acute - on - chronic (subacute) liver failure were divided into early stage, middle stage, and advanced stage groups. There were significant differences in IL - 6 and TNFɑ decreases between the early stage, middle stage, and advanced stage groups (P < 0.05). IFNɣ decrease significantly difered between the early stage or middle stage group and advanced stage group (P < 0.05), but not between the former two groups (P > 0.05). After PE therapy, 29 out of 36 liver failure patients recovered well, while the remaining 7 patients deteriorated. The recovered group had significantly greater decreases in TNFɑ (122.58 ± 57.64 ng/L vs 42.45 ± 19.86 ng/L, P < 0.05), IL - 6 (26.93 ± 7.25 ng/L vs 6.71 ± 3.55 ng/L, P < 0.05), and IFNɣ (284.06 ± 94.31 ng/L vs 217.47 ± 45.76 ng/L, P < 0.05) than the deteriorated group. The response rates of patients with acute, subacute, acute - on - chronic, and chronic liver failure were 66.67%, 85.71%, 86.36%, and 50%, respectively. Conclusion Continuous artificial liver PE therapy can effectively remove pro - inflammatory cytokines from plasma and thus improve the clinical outcomes of patients with liver failure. [ABSTRACT FROM AUTHOR]

Objective To investigate the relevance between relapse and course of treatment in genotype 1 chronic hepatitis C (CHC) patients with slow virologie response. Methods Totally 157 genotype 1 CHC inpatients or outpatients were selected from the Third People's Hospital of Jiaozuo and the People's Hospital of Jiaozuo from April 2010 to March 2013. The patients were treated with interferon ɑ - 1b in conjunction with ribavirin. At 6 months of the initial treatment, 51 patients with slow virologic response were randomly divided into groups A (24 cases) and B (27 cases), which received continued treatment for another 6 and 12 months, respectively. A1 - year follow - up of patients was performed after withdrawal of treatment. Adverse reactions in patients receiving the antiviral therapy were assessed. Furthermore, group comparisons were performed on the clearance rate of hepatitis C virus (HCV) RNA and normalization rate of alanine aminotransferase (ALT) at withdrawal of treatment, as well as the CHC relapse rate and ALT normalization rate at 6 months and 1 year after withdrawal of treatment. Continuous data were compared using t test, and categorical data were compared using χ² test. Results The rate of adverse reactions in patients receiving antiviral therapy had no significant difference between groups A and B (P > 0.05). At withdrawal of treatment, there were no significant diferences between groups in terms of HCV RNA clearance rate(95.65% vs 92.59%) and ALT normalization rate (95.65% vs 88.89%) (χ² = 0.02 and 0.13, respectively, both P > 0.05). At 6 months and 1 year after withdrawal of treatment, the CHC relapse rate in group B was significantly lower than that in group A (20.00% vs 50.00%, = 4.69, P < 0.05; 36.00% vs 68.18%, χ² = 4.85, P < 0.05); the ALT normalization rate in group B was nonsignificantly higher than that in group A (84.00% vs 59.09%, χ² = 3.63, P > 0.05; 72.00% vs 50.00%, χ² = 2.40, P > 0.05). Conclusion To extend the 6 - month course of treatment can significantly reduce the relapse following slow virologic response in patients with genotype 1 CHC. [ABSTRACT FROM AUTHOR]

Objective To evaluate the efficacy of 0.1% tacrolimus ointment and 0.1% mometasone furoate cream in the treatment of chronic actinic dermatitis （CAD）. Methods Forty male patients with CAD were recruited and divided into two groups randomly．Twenty cases were treated with 0.1% tacrolimus ointment（Group A），and the other 20 cases were treated with 0.1% mometasone furoate cream（Group B） . The medications mentioned were applied topically to the lesions on the face twice a day and mizolastine tablet 10 mg per day given orally for 4 weeks. The therapeutic efficacy and side effects of medications were observed. The enzyme linked immunosorbent assay（ELISA） method was used to measure the serum levels of IFN-γ，IL-2 and IL12 in CAD patients before and after treatment with topical tacrolimus ointment and mometasone furoate cream. Results （1） Both groups had overall response rates of 100%， with no statistically significant difference（ > 0.05） . （2） Serum levels of IFN-γ，IL-2 and IL-12 were down-regulated after treatment in both treatment groups respectively （ < 0.01） . No statistically significant difference was found between the two treatment groups（ > 0.05） . Conclusion 0.1% tacrolimus ointment is effective in the treatment of CAD. Its therapeutic efficacy is equivalent to that of 0.1%mometasone furoate cream. It can be used as a possible steroid sparing equivalent. [ABSTRACT FROM AUTHOR]

Objective: To observe the effects of pricking and cupping therapy combined with Chinese herbal wet compress on Th1/Th2 balance and the concentration of substance P (SP) in treatment of patients with herpes zoster at acute stage and explore the mechanism of clinical efficacy of this combined therapeutic method., Methods: The patients of herpes zoster at acute stage were randomly divided into a treatment group (58 cases), a control group No.1 (57 cases), a control group No.2 (58 cases) and a control group No.3 (59 cases). In the treatment group, the therapeutic regimen was pricking and cupping+Chinese herbal wet compress+basic treatment. In the control group No.1, pricking and cupping + basic treatment were administered. In the control group No.2, Chinese herbal wet compress+basic treatment were provided. In the control group No.3, only basic treatment was delivered. The treatment duration was 9 days in each group. The score of pain degree, the score of sleep quality and the rehabilitation conditions of pain and herpes were observed in 4 groups. The concentration of serum interleukin 4 (IL-4), interferon gamma (IFN-γ) and SP were detected by ELISA. The clinical efficacy was evaluated. The occurrence of adverse reaction during treatment was observed., Results: After treatment, the score of pain degree and the concentrations of serum IL-4 and SP were all lower than those before treatment in 4 groups ( P <0.05); and the score of subjective sleep quality, the concentrations of serum IFN-γ and IFN-γ/IL-4 were all increased in comparison with those before treatment ( P <0.05). In the treatment group and the control groups No.1 and No.2, the score of pain degree and the concentrations of serum IL-4 and SP were all lower than those in the control group No.3 ( P <0.05); and the score of subjective sleep quality, the concentrations of serum IFN-γ and IFN-γ/IL-4 were higher than those in the control group No.3, respectively ( P <0.05). Besides, every indicator in the treatment group was improved more significantly than that either in the control group No.1 or the control group No.2 ( P <0.05). After treatment, compared with the control group No.3, the time of pain relief and disappearance, and the time of blister termination, incrustation and decrustation were all shorter in the treatment group, the control group No.1 and the control group No.2 separately ( P <0.05); and every indicator in the treatment group was reduced more significantly in comparison with either the control group No.1 or the control group No.2 ( P <0.05). The total effective rate in the treatment group (96.55%, 56/58), the control group No.1 (92.98%, 53/57) and the control group No.2 (91.38%, 53/58) was higher than that in the control group No.3 (74.58%, 44/59, P <0.05). No any adverse reaction occurred in the 4 groups., Conclusion: The pricking and cupping therapy combined with Chinese herbal wet compress effectively promotes the recovery of Th1/Th2 balance and reduces the concentration of serum SP in patients with herpes zoster at acute stage. This combined therapeutic regimen is conductive to the recovery of patients, the improvement of therapeutic effects and the decrease of the risk of pos-therpetic neuralgia.