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Objective: To establish the animal model of hemisection of spinal cord injury joint bridge of rats for the study of spinal cord injury. Methods: The rat model of hemisection of spinal cord injury was established, then making joint bridge by median nerve of rat's forelimb. Four weeks later, left ventricular of rats was perfused, fixed and coveraged. The expression of GFAP, RECA, NF-200 was detected by immunohistochemistry; the others rats were detected using tannic acid-ferric chloride perfused method. Finally, the situation of neovascularization in the grafts and the relation between neovascularization and peripheral nerve fibers was detected. Results: The results of RECA and HE staining after perfusion with tannic acid-ferric chloride showed that the neovascularization was detectable in the grafts, and the blood could reach the inside of the grafts. Some NF-positive nerve fibers were co-localized with GFAP-positive immunoreactivity. Conclusions: The neovascularization from host can reach the inside of graft after hemisection of spinal cord injury joint bridge by median nerve, which is beneficial to the survival and growth of nerve fibers. The animal model of spinal cord hemisection joint bridge could provide the basis for the survival of peripheral nerve and its further study. [ABSTRACT FROM AUTHOR]