Search

Copyright of Mycosystema is the property of Mycosystema Editorial Board and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Objective: To discuss the host proteins that interact with the Nelson Bay orthoreovirus (NBV)σNS protein in the fibroblasts L929 of the mice, and to clarify the effect of host proteins on the viral replication. Methods: The bait plasmid pGBKT7-S3 expressing σNS protein was constructed, and sequencing technology was used to verify the accurate expression of the bait plasmid in the Y2H yeast cells. The pGBKT7-S3 and pGADT7 plasmids were separately and jointly transformed into the Y2HGold yeast cells, plated on solid medium, and the colony growth was observed to confirm that the σNS protein was non-toxic to the yeast cells and could not self-activate the reporter gene. The bait plasmid pGBKT7-S3 was hybridized with the cDNA library in fibroblasts L929 of the mice, and the plasmids encoding the interacting proteins were extracted from the positive clones. The positive sequencing results were screened for the proteins interacting with NBV σNS through the Uniprot database. Gene Ontology(GO) functional enrichment analysis, Kyoto Encyclopedia of Genes and Genomes(KEGG) signaling pathway enrichment analysis, and STRING bioinformatics analysis were performed on the interacting proteins. Results: A total of 61 positive clones were successfully screened. The DNA sequencing analysis and BLAST alignment removed 23 positive clones that did not match the database or were similar in sequence. The positive sequencing results identified 38 proteins interacting with NBV σNS through the Uniprot database. Thirty-one proteins were involved in cellular biological processes; thirty-six proteins were cellular anatomical components; thirty-one proteins had binding functions. Five proteins were part of the mitochondrial respiratory chain; seven proteins were ribosomal proteins and components of the ribosomal subunits; two proteins were involved in iron metabolism homeostasis. The GO functional enrichment analysis results showed that the interacting proteins were enriched in biological processes(BP) such as cellular processes, metabolic processes, biological regulation, localization, and response to stimuli; the cellular components were mainly cellular anatomical components and protein-containing complexes; the molecular functions were concentrated in binding, catalytic activity, structural molecule activity, and transporter activity. The KEGG signaling pathway enrichment analysis results showed that the proteins were highly enriched in translation, folding, sorting, and degradation pathways of genetic information processing and were mainly associated with the digestive system in the organism; they were linked to various viral infections and cancers. The STRING analysis results showed that the interacting proteins included ribosomal proteins, protein modification proteins, metabolic proteins, and immune proteins. Conclusion: The host proteins that interact with NBV σNS protein are successfully screened, and these host proteins play important roles in viral replication. [ABSTRACT FROM AUTHOR]

Copyright of Journal of Agricultural Science & Technology (1008-0864) is the property of Journal of Agricultural Science & Technology and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Journal of Henan Agricultural Sciences is the property of Editorial Board of Journal of Henan Agricultural Sciences and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of China Brewing is the property of China Brewing Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Telecommunication Engineering is the property of Telecommunication Engineering and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Forensic Science & Technology is the property of Institute of Forensic Science, Ministry of Public Security and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Acta Edulis Fungi is the property of Acta Edulis Fungi and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Journal of Northwest A & F University - Natural Science Edition is the property of Editorial Department of Journal of Northwest A&F University (Natural Science Edition) and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Journal of Taiwan Agricultural Research is the property of Taiwan Agricultural Research Institute, Council of Agriculture, Executive Yuan and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Nutritional Sciences Journal is the property of Nutrition Society of Taiwan and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Journal of Henan Agricultural Sciences is the property of Editorial Board of Journal of Henan Agricultural Sciences and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Mycosystema is the property of Mycosystema Editorial Board and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Mycosystema is the property of Mycosystema Editorial Board and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

The pathogenic fungi of postharvest grey mold on cherry fruits from Hanyuan, Sichuan Province, and Yantai, Shandong Province were collected and identified by single-spored method. The sensitivity of the tested strains to thiophanate-methyl, diethofencarb, and procymidone was determined by differential dose method. The alignments of the DNA sequences of β-tubulin gene and BcOS1 gene were carried out to analyze the molecular mechanism of resistant strains. The results showed that the 54 isolates collected from postharvest cherry were Botrytis cinerea, and the total resistance frequency to thiophanate-methyl was 79.6%. Among them, the resistance frequency of thiophanate-methyl-resistant and diethofencarb-sensitive strains (BEN R1) was 25.9%. The resistance frequency of strains resistant to both thiophanate-methyl and diethofencarb (BEN R2) was 53.7%. Nine procymidone-resistant strains (DCF R) were detected, and the resistance frequency was 16.7%. There were two types of mutation in β-tubulin gene of benzimidazole-resistant strains. In BEN R1, the codon at position 198 was mutated from GAG to GCG, resulting in the amino acid encoded by Glutamate-Glu (E) being changed to Alanine-Ala (A). In BEN R2, the codon at position 198 was mutated from GAG to GTG, resulting in the change of the encoded amino acid from Glu (E) to valine-Val (V). The codon at position 365 in BcOS1 gene of DCF R was mutated from ATC to AAC or AGC, resulting in the change of the encoded amino acid from isoleucine-Ile (I) to asparagine-Asn (N) or serine-Ser (S). The results indicated that B. cinerea on cherry had different degrees of resistance to thiophanate-methyl and procymidone. It is necessary to alternate with other types of fungicides while strengthening resistance monitoring to delay the development of resistance. [ABSTRACT FROM AUTHOR]

Copyright of Journal of Chinese Institute of Food Science & Technology / Zhongguo Shipin Xuebao is the property of Journal of Chinese Institute of Food Science & Technology Periodical Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Acta Agriculturae Zhejiangensis is the property of Acta Agriculturae Zhejiangensis Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

To screen DNA barcodes suitable for the identification of walnut cultivars and related species that were not easily distinguished by morphology, so as to provide a new way for accurate identification of walnut cultivars and ensure the healthy development of walnut industry, 11 walnut cultivars and 5 relatives were used as test materials, DNA barcodes with high amplification efficiency and abundant variation sites among cultivars were selected by amplification, sequencing and sequence variation analysis in three chloroplast genes (rbcL, psbA-trnH, matK) and one nuclear DNA sequence (ITS1). The results showed that, the success rates of amplification and sequencing of the four candidate DNA barcodes in 16 species (cultivars) were 100%. The rbcL sequences showed minimal differences among species (cultivars), while the psbA -trnH sequences differed only among genera. matK and ITS1 sequences differed among species (cultivars), and their similarities were 99. 12% and 99. 88%, with 18 and 8 base site variants, respectively. Different species (cultivars) clustered differently in the phylogenetic tree constructed based on matK sequence, which verified the accuracy of using matK to identify walnut cultivars and their relatives. Thus, the combination of matK+ITS1 barcode can effectively identify walnut cultivars and their relatives. [ABSTRACT FROM AUTHOR]

Copyright of Chinese Journal of Preventive Veterinary Medicine / Zhongguo Yufang Shouyi Xuebao is the property of Chinese Journal of Preventive Veterinary Medicine Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of China Brewing is the property of China Brewing Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of China Brewing is the property of China Brewing Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Shipin Kexue/ Food Science is the property of Food Science Editorial Department and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Mycosystema is the property of Mycosystema Editorial Board and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Bulletin of Botanical Research is the property of Bulletin of Botanical Research Editorial Department and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Acta Agriculturae Zhejiangensis is the property of Acta Agriculturae Zhejiangensis Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Acta Agriculturae Zhejiangensis is the property of Acta Agriculturae Zhejiangensis Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Objective To assess the feasibility of the rbcL sequence of chloroplast DNA as a genetic marker to identify Cannabis sativa L. Methods The rbcL sequences in 62 Cannabis sativa L. samples, 10 Humulus lupulus samples and 10 Humulus scandens DNA samples were detected, and 96 rbcL sequences of the Cannabaceae family were downloaded from Genbank. Sequence alignment was performed by MEGA X software, the intraspecific and interspecific Kimura-2-Parameter （K2P） genetic distances were calculated, and the system clustering tree was constructed. Results The rbcL sequence length acquired by sequencing of Cannabis sativa L. and Humulus scandens were 617 bp and 649 bp, respectively, and two haplotypes of Cannabis sativa L. were observed in the samples. The BLAST similarity search results showed that the highest similarity between the sequences acquired by sequencing and Cannabis sativa L. rbcL sequences available from Genbank was 100%. The genetic distance analysis showed that the maximum intraspecific genetic distance （0.004 9） of Cannabis sativa L. was less than the minimum interspecific genetic distance （0.012 9）. The results of median-joining network and system clustering tree analysis showed that Cannabis sativa L. and other members of the Cannabaceae family were located in different branches. Conclusion The rbcL sequence could be used as a DNA barcode for identifying Cannabis sativa L., and combined with comparative analysis of the rbcL sequence and system cluster analysis could be a reliable and effective detection method for Cannabis sativa L. identification in forensic investigation.

Copyright of Food Research & Development is the property of Food Research & Development Editorial Department and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Environmental Science & Technology (10036504) is the property of Editorial Board of Environmental Science & Technology and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Mycosystema is the property of Mycosystema Editorial Board and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

[Objective] In order to supply theoretical basis for the resource protection and genetic improvement of Schizothorax kozlovi, the genetic diversity of one cultured population from Bijie, Guizhou was assessed by mitochondrial control region (D-loop） segments and Cyt b gene sequences. [Method] The partial sequences of D-loop region and Cyt b gene in farmed S. kozlovi were obtained by PCR and DNA sequencing, then sequence variation and genetic diversity of the two sequences were analyzed using related softwares. [Result] 9 and 34 polymorphic loca, as well as 7 and 8 haplotypes were detected in the sequences of D-loop region and Cyt b gene respectively, the haploid type diversity (Hd) and nucleotide diversity（π） were 0.765, 0.842 and 0.00 519, 0.00 826 respectively, on the basis of the sequences of D-loop region and Cyt b gene in the population. Analysis of neutral detection and nucleotide mismatch distribution showed that there might be no expansion ocurred recently in the population of S. kozlovi. [Conclusion] High genetic diversity was observed in the farmed S. kozlovi from Bijie, Guizhou. The result of selective pressure tests infered that negative selection played a positive role in the Cyt b gene of farmed S. kozlovi, that was, artificial selection served limited functions. [ABSTRACT FROM AUTHOR]

To character the codon usage patterns of chloroplast genome in alfalfa, forty-nine coding DNA sequences were analyzed using CodonW, CUSP, CHIPS and SPSS softwares. The results were as follows:(1)The average GC content at the 3rd codon position was 26.44% in the alfalfa chloroplast genome. The effective codon number(ENC)ranged from 40.6 to 51.41, indicating that codon bias was weak in the alfalfa chloroplast genome.(2)Thirty codons showed relative synonymous codon usage(RSCU)value greater than 1, including 29 codons ending with A and U, which suggested that the third codon position preferred A or U.(3)Neutrality analysis showed no significant correlation was observed between GC3 and GC12, suggesting natural selection mainly contributed to the codon usage bias. ENC-plot analysis showed that there were some genes with low ENC values lying below or near the expected curve, which indicated that mutation also affected the formation of codon usage bias. In addition, 17 codons were identified as optimal codons in alfalfa. We concluded that the codon usage bias of alfalfa is formed under effect of natural selection and mutational bias. This study will lay a good foundation for development of chloroplast genetic engineering and molecular breeding of alfalfa. [ABSTRACT FROM AUTHOR]

Copyright of West China Journal of Stomatology is the property of Sichuan University, West China College of Stomatology and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Gene editing, also known as genome editing or genome engineering, is a technique that introduces mutations in DNA sequences in the form of insertion, deletion, or base substitution. There are many types of gene editing techniques, such as zinc-finger nucleases(ZFNs), transcription activator-like effector nucleases(TALENs)and clustered regularly interspaced short palindromic repeats/CRISPR-associated nuclease 9(CRISPR/Cas9). CRISPR/Cas9 has developed rapidly in recent years. The emergence of gene editing techniques has accelerated the development of plant functional genomics and has great potential in precision crop breeding. Plant organelle gene editing mainly refers to editing plant mitochondrial and chloroplast genomes. Plant mitochondrion and chloroplast are often referred to as the “power house” and “production workshop”, respectively, due to their importance in central metabolic functions. Editing mitochondrial and chloroplast genomes will improve the understanding of the genetic function of these genomes and develop their applications in crop improvement and industrial production. At present, organelle gene editing techniques have emerged and have a very broad application prospect. In this review, we summarized the development of gene editing techniques, structures and characteristics of plant organelle genomes, mitochondrial gene editing, and chloroplast genetic transformation, and finally we proposed the future research directions and prospects of organelle gene editing. [ABSTRACT FROM AUTHOR]

Copyright of Journal of Henan Agricultural Sciences is the property of Editorial Board of Journal of Henan Agricultural Sciences and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

In order to find a rapid and efficient identification method for three original species of Curcumae Rhizoma, identification efficiency of different DNA barcoding sequences were evaluated in present study. Totally nine samples of three different species of Curcuma were collected. After DNA extraction, PCR amplification and sequencing, seven kinds of DNA barcoding sequences, including ITS, ITS2, matK, psbA-trnH, trnL-trnF, rpoB and atpB-rbcL, were firstly compared in terms of success rate of PCR amplification and characteristics of sequences. Then, by means of variation site analysis and genetic distance calculation, these seven kinds of DNA barcoding sequences were further evaluated. Finally, the unidentified samples were identified by the phylogenetic tree based on the chosen DNA barcoding sequences. The results were as follows:(1)ITS, ITS2 and matK barcoding sequences were inapplicable due to the low success rate of PCR amplification and sequencing; The variation information of psbA-trnH, trnL-trnF and rpoB was insufficient to distinguish three different original species of Curcumae Rhizoma; Only atpB-rbcL barcoding sequence was 642-645 bp in length with 29.0%-29.9% GC content and 11 variation sites, for which, the three species of Curcumae Rhizoma could be distinguished only by atpB-rbcL barcoding sequence.(2)According to the phylogenetic tree based on atpB-rbcL sequence, the unidentified samples were identified as Curcuma wenyujin. In conclusion, atpB-rbcL sequence could be used as a standard sequence for the rapid and efficient identification of original species of Curcumae Rhizoma. [ABSTRACT FROM AUTHOR]

Copyright of Journal of Henan Agricultural Sciences is the property of Editorial Board of Journal of Henan Agricultural Sciences and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Objective To explore the value of Metagenomic Next-generation Sequencing (mNGS) in the detection of pathogens in sterile sites of patients with suspected infectious diseases. Methods A total of 672 patients with suspected infectious diseases by bacterial culture and mNGS admitted to The Fifth Affiliated Hospital of Sun Yat-sen University from January 2018 to November 2019 were retrospectively analyzed. The positive rate and influencing factors (antibiotic exposure, specimen type), pathogen distribution, department distribution. Results The culture positive rate was 8.6%, metagenomic sequencing was 35.4%, and bacterial, viral, fungal, rickettsial, parasitic and Mycoplasma / chlamydia were detected in 43.7%, 21.9%, 15.4%, 16.1%, 1.8% and 1.1% of samples with metagenomic sequencing positive from 672 patients, respectively; the positive rates before and after antibiotic use were 32.9% and 37.9%, respectively (P>0.05). The constituent ratios of blood, cerebrospinal fluid, tissue, joint puncture fluid and other specimens the positive detection rates were 40.5%, 24.1%, 45.0%, 36.8% and 30.4%, respectively (P<0.05). Infection department hematology, neurology, orthopedics, respiratory medicine, ICU and other departments, respectively, and the positive detection rates were 42.9%, 34.2%, 15.7%, 36.7%,28.6%, 21.1% and 39.0%, respectively (P<0.05). Consistency analysis of mNGS and culture: 41 out of 672 patients were positive for mNGS and culture, of which 31 were consistent with mNGS and 10 were inconsistent with mNGS; among 434 samples with negative mNGS, 17 were culture positive. Conclusion Metagenomic Next-generation Sequencing improves the detection rate of infectious diseases pathogens, especially for viruses, Rickettsia, parasites, Mycobacterium tuberculosis and rare pathogens, but the detection results of Metagenomic Next-generation Sequencing should be combined with epidemiological and clinical characteristics to comprehensively evaluate whether it is a pathogen. Antibiotics had little effect on the positive rate of mNGS. [ABSTRACT FROM AUTHOR]

Copyright of Food Research & Development is the property of Food Research & Development Editorial Department and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

To comprehend the codon usage pattern of chloroplast genome in Dalbergia odorifera, the 52 coding DNA sequences were analyzed to obtain to the results of neutrality plot, ENC-plot and PR2-plot analysis using Codon W 1.4.2 and online software CUSP in the present study. The results were as follows: The GC content in the three positions of codons from the chloroplast genome of D. odorifera was GC1(46.01%)>GC2(38.98%)>GC3(27.80%)successively. The range of effective number codon was from 37.66 to 54.43, and there were 37 genes when ENC value was greater than 45, and there were 29 genes when RSCU value was greater than 1, including 16 genes ending with U and 12 genes ending with A. These suggest that the codons prefer ends with A and U, and have a weak bias. The neutrality plot showed that there was no significant correlation between GC3 and GC12, the correlation coefficient was 0.250, and the regression coefficient was 0.394; ENC-plot analysis revealed that there were 38 genes which ENC ratio located beyond the section from -0.05 to 0.05; PR2-plot analysis showed that U>A and G>C in the base usage frequency. These all illustrated that the codon usage bias in the chloroplast genome of D. odorifera was mainly affected by mutation; 19 codons were identified as the optimal codon. The present study could be useful in the chloroplast genetic engineering and genetic diversity analysis of D. odorifera. [ABSTRACT FROM AUTHOR]

【Objective】The present paper aimed to identify the augophagy(ATG) gene of Vicia faba L. based on the results of transcriptional sequencing, and analyze its response to drought stress, which provides theoretical reference for further study on the mechanism of these genes under drought stress.【Method】According to the annotation of Swiss port, NCBI,ensemble and other databases, the ATG gene of V. faba L. was screened and identified based on RNA sewuencing data. The conserved domain, subcellular localization prediction, phylogenetic tree and motif were analyzed by SMART,MEGA-X and MEME software. Based on transcriptome data, the expression differences of ATG gene in V. faba L. varieties CDAS105(drought tolerant) and ECAN No.1(sensitive) were analyzed under drought stress.【Result】 Twelve ATG genes from V.faba L. were screened and annotated, all of which contained autophagy domain. According to the different C-terminal domains, they were divided into five subfamilies: ATG3,ATG6,ATG8,ATG18 and ATG26. In the phylogenetic tree constructed by faba bean ATG gene and Arabidopsis,maize, rice and yeast, it was found that genes with the same C-terminal domain were clustered together. Prediction of subcellular localization indicated the elevent genes and one ATG gene were located in extracellular and cytoplasm, respectively. Gene structure analysis showed that there were no introns in the DNA sequences of ATG family genes in V. faba L.,and they were composed of UTR and CDs regions. Conserved motif analysis identified that these proteins contained 20 mortifs. Analysis of ATG expression showed that ATG18 b,ATG18 c and ATG8 a were up-regulated in drought resistant varieties after 16 hours or 64 hours drought treatment, and the expression of ATG8 a in drought resistant varieties was further enhanced with the increase of drought treatment time. 【Conclusion】Twelve ATG genes were identified and distributed in five subfamilies. ATG8 a, ATG18 b and ATG18 c responded to drought stress, which could be used as potential gene resources to improve the drought tolerance during seed germinating in faba bean. [ABSTRACT FROM AUTHOR]

Copyright of Journal of Forensic Medicine / Fayixue Zazhi is the property of Journal of Forensic Medicine Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Journal of Food Safety & Quality is the property of Journal of Food Safety & Quality Editorial Department and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of China Sciencepaper is the property of China Sciencepaper and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Journal of Agricultural Science & Technology (1008-0864) is the property of Journal of Agricultural Science & Technology and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Farnesyl diphosphate synthase(FPS)is a key enzyme in the biosynthesis of triterpene saponins, it can promote the biosynthesis triterpenoid saponins in plants. In order to study the function of FPS gene in Ilex cornuta, we isolated a FPS gene from Ilex cornuta, and carried out bioinformatics analysis and expression analysis. In this study, PCR was used to isolate the cDNA sequence of a FPS gene from the leaves of I. cornuta, and the gene was named as IcFPS1. DNA sequencing results showed that the amplified cDNA of IcFPS1 gene was 1 591 bp. Sequencing analysis revealed the IcFPS1 gene contained a complete open reading frame, and the open reading frame was 1 029 bp in length, encoding 342 amino acids. The molecular weight and isoelectric point of predicted IcFPS1 protein were 39.58 kDa and 5.18, respectively. Physical and chemical properties analysis revealed that IcFPS1 protein was a hydrophilic protein, and it did not contain a signal peptide, and had no transmembrane region of the IcFPS1 protein. The multiple alignment of FPS sequences with BLASTP and Align X revealed that the IcFPS1 protein was highly homologous to other known FPS proteins from different plant species, and they had common conserved regions and amino acid sequences. The similarity between the IcFPS1 and the Panax quinquefolius FPS sequence was as high as 89%. Phylogenetic tree analysis showed that the FPS protein of the Ilex cornuta was closely related to FPS proteins of the Araliaceae family belonging to the angiosperm. These results indicate that the FPS gene is conserved during evolution. Protein-protein interaction network analysis showed that the IcFPS1 protein is involved in the synthesis pathway of isoprenoids, which may be the same as IPP1, IPP2, GGPS3, GGPS6 and ERA1 proteins. Real-time quantitative PCR analysis showed that IcFPS1 gene was expressed in all tested tissues of Ilex cornuta, while the expression level of IcFPS1 gene in different tissues was different. IcFPS1 gene had the highest expression in roots, but was less expressed in the stems and female flowers of Ilex cornuta. [ABSTRACT FROM AUTHOR]

Copyright of Shipin Kexue/ Food Science is the property of Food Science Editorial Department and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Background and objective It has been proven that epidermal growth factor receptor (EGFR) mutation is the most important predictive factor for determining the effect of EGFR tyrosine kinase inhibitors (TKIs) applied to non-small cell lung cancer (NSCLC) patients. The patients with EGFR mutations response better to TKIs. To detect EGFR mutation has been particularly essential to select first-line treatment for lung cancer patients. To research and analyze the sensitivity and specificity of immunohistochemistry (IHC) using mutation specific antibodies in detecting EGFR mutations compared with DNA sequencing, and further evaluate the accuracy and clinical application value of IHC. Methods All required articles in Pubmed database were searched. The deadline of retrieval was March 26, 2013. Then further screening the articles based on the inclusion and exclusion criteria. Meta analysis of diagnostic test was applied to analyze the sensitivity and specificity of IHC compared with DNA sequencing for the detection of EGFR mutations. Results Ten articles were included in the meta analysis, there were 1,679 samples in L858R group and 1,041 samples in E746-A750del group. The DOR were 225.17 (95%CI: 55.67-910.69) and 267.16 (95%CI: 132.45-538.88) respectively; the AUC of SROC were 0.948,4 (SEAUC=0.014,4) and 0.981,3 (SEAUC=0.009,9) respectively; the Q values were 0.888,3 (SEQ*=0.019,2) and 0.939,7 (SEQ*=0.019,1) respectively. Conclusion The specificity and sensitivity of IHC method using these two mutation-specific antibodies were relatively high. As a screening method for EGFR mutations, the IHC with mutation specific antibodies is of clinical value.

Copyright of Journal of Agricultural Science & Technology (1008-0864) is the property of Journal of Agricultural Science & Technology and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Objective: To explore the genetic mutation mechanism of a rare Rhesus D variant individual., Methods: Regular serological assay was used for determination of Rh type for the sample. Indirect anti-human globulin test (IAT) was used to confirm the RhD antigen and screen the antibodies. D-screen reagent was used to analyze the RhD epitopes of the sample. RHD genotype and RHD zygosity testing of the sample were detected by palymerase chain reaction with sequence-specific primers (PCR-SSP). The full length coding region of RHD gene was sequenced. RHD mRNA was detected using reverse transcription polymerase chain reaction (RT-PCR). The PCR products were cloned and sequenced., Results: The RhD blood group of the sample was determined as weak D, and the Rh phenotype was CcDEe. The antibody screening was negative. The sample tested with all monoclonal anti-Ds in D-screen showed the D epitope profiles as partial D types. The analysis of RHD gene sequence indicated that the individual with RHD c.845G/A and RHD c.1227G/A base heterozygosis. Three kinds of alternative splicing isoforms were obtained by TA cloning and sequencing., Conclusion: The object has RHD c.845G/A and RHD c.1227G/A mutation. This heterozygous mutation is responsible for the low expression of RhD antigen on the red blood cells of the sample.