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【Objective】In order to provide favorable genes for the analysis of the grafting healing mechanism of Cucumis sativus L. or Cucurbita moschata Duch. grafted seedlings and the molecular breeding of resistance of the cucumber and pumpkin vegetables, we conducted the analysis of the expression patterns of Bcl-2 associated athanogene（BAG）family proteins in the cucumber and pumpkin in response to abiotic stress and in response to light during cucumber or pumpkin grafting healing.【Method】Based on the genome information of the cucumber and pumpkin, the BAG gene family in the cucumber and pumpkin was identified through bioinformatics methods, and its physicochemical characteristics, chromosome mapping, gene structure, phylogeny and collinearity were analyzed. The expression patterns of BAG family proteins in the cucumber and pumpkin in response to abiotic stress and in response to light during cucumber or pumpkin grafting healing were analyzed. These data were based on the public database and the transcriptome sequencing data of the cucumber or pumpkin grafted seedlings in the process of grafting and healing.【Result】A total of 12 and 18 members of the BAG family were identified in the cucumber and pumpkin, respectively and they were all divided into two subfamilies and the gene members were highly conserved. The BAG of subfamily I was mainly involved in gene regulation and stress response, while the BAG of subfamily II was mainly involved in plant development. The BAG genes of cucumber and pumpkin had multiple linear relationships with Arabidopsis, Oryza sativa L. and Solanum lycopersicum L., respectively, but the CsaV3_1G017210 was relatively conservative and there was no collinearity with the BAG genes in Arabidopsis, rice, tomato and pumpkin. Different BAG genes possessed tissue-specific expression patterns. CsaV3_6G000970 and CmoCh08G008520 belonged to the BAG family molecular chaperone regulator 6. In both cucumber and pumpkin, they were upregulated during the grafting healing process and in response to abiotic stress.【Conclusion】BAG family genes respond differently to abiotic stress and light during the grafting healing process of the cucumber or pumpkin. These genes synergistically regulate the growth, development and grafting healing of the cucumber and pumpkin. BAGs play an important role in the process of the cucumber and pumpkin abiotic stress and cucumber or pumpkin grafting healing. [ABSTRACT FROM AUTHOR]

[Objective] The paper aims to explore whether calmodulin-like CMLs participate in the sexual differentiation process in Cucumis sativus L., so as to provide reference for improving the regulatory network of sexual differentiation furtherly. [Method] In the study, CMLS, ACSIG, ACS2 and ACS11 genes of cucumber were cloned, qPCR was used to detect the expressions of CMLS gene in different types of the flowers, and the interactions between CMLS and proteins encoded by major genes of cucumber sex differentiation were detected by the method of yeast two-hybrid. [Result】 CML8 was cloned from cucumber which contained a complete ORF of 441 bp, encoding 146 aa without signal peptide. It was an extracellular protein without transmembrane domain and introns, and contained 4 EF-hand domains with Ca2+ binding ca- pacity, and subcellular was located in cell membrane and cytoplasm. ACSIG, ACS2 and ACSII, three key genes of cucumber sexual differ- entiation were cloned, and sequence alignment showed that the nucleotide sequences and encoded protein sequences of the three genes were consistent with those in the cucumber database. There were significant differences in the expression levels of three genes in the female flow- ers, male flowers and bisexual flowers, and extremely significant differences in male flowers. The yeast two-hybrid experiment showed that CMLS could interact with ACSIG and ACS2, but not with ACS11. [Conclusion] Calmodulin-like 8 (CMLS) was cloned, and CMLS could interact with ACSIG and ACS2, which might participate in the sexual differentiation of cucumber, and the study was the first time to explore the involvement of calmodulin in the process of plant sexual differentiation, and expect to provide reference for calcium signaling system to participate in the process of plant sexual differentiation. [ABSTRACT FROM AUTHOR]

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According to the second part of NY/T761—2008, the vinclozolin residue in cucumber was determined by GC. The sources of uncertainty components coming from the measuring process was analyzed. The mathematical model for the evaluation of measurement uncertainty was built and the uncertainty components were synthesized and expanded. The results showed that the relative combined standard uncertainty and expanded uncertainty of measurement results were 2.78% and 5.56% (k=2), respectively. The sources of measurement uncertainty mainly derived from diffusion and constant volume process of standard solution, the recovery rate in the extraction and purification process, and the error caused by the measurement of chromatographic peak area. The results of this study provide a reference for objective evaluation of the pesticide residue detemanation results. [ABSTRACT FROM AUTHOR]

Copyright of Journal of Agro-Environment Science is the property of Journal of Agro-Environment Science Editorial Board and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Journal of Agricultural Science & Technology (1008-0864) is the property of Journal of Agricultural Science & Technology and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

In order to investigate the effect of soil amendment (SA) togetlier witli entomopatliogenic nematode (EPN，Heterorhabditis beicher-riana) on root-lcnot nematodes (Meloicdogyne incognita) and the growth of cucumber plants，Qian-qiu No. 1，as the tested cucumber cultivar，was chosen to be studied under lab condition.，through artificial inoculation of root-knot nematode. The second juveniles (J2) of root-knot nematode and the thmd juveniles (J3) of EPN were observed after 7，14 and 21 days， respectively. Moreover， the leaf area，plant height，fresh weight and root knot number of cucumber seedling were checked after 30 days. The resialts demonstrated that thie survival numbers of root knot nematodes (J2) in trials treated by SAand EPNwere significantly lower than that in control trial with just water (M) after 7，14 and 21 days，respectively. After 21 days，the death number of root knot nematodes in SAtrial was about twice of that in in control tri-al，while the death number of root knot nematodes in EPNtrial was about three times of that in in control trial. However，the survival rate of EPNin SAtreatment trial was obviously higher than those in other trials all through the experimental period. Moreover，SAtogether with EPN could increase the leaf area，fresh weight，plants height of cucumber seedling，but decreased the root-knot number within certain length of cucumber root. Above all， it was concluded that SAtogether with EPNcould improve the resistant ability of the plants against root knot nematodes and reduce the damage caused by root knot nematodes，which provided theoretical basis for future stiady on efective amendment technolog on soil contaminated byroot-knot nematodes. [ABSTRACT FROM AUTHOR]

Copyright of Journal of Southern Agriculture is the property of Journal of Southern Agriculture and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)