Search

AIM: To investigate the expression of inducible nitric oxide synthase （iNOS） and CD163 in periapical tissues at varying stages of human chronic apical periodotitis （CAP）. METHODS: Forty-five voluntary samples were collected and categorized into three groups based on the degree of CAP: healthy control group （n=15）, chronic apical abscess group （n=15）, and periapical cyst group （n=15）. Double immunofluorescence staining was employed to examine the expression of CD68+-iNOS+ and CD68+-CD163+ cells in periapical tissues. RESULTS: （1） The densities of CD68+- iNOS+ and CD68+-CD163+ cells showed significant differences among CAP groups （P<0. 01）. （2） The density of CD68+- iNOS+ cells in chronic apical abscess group was significantly higher than that in periapical cyst group （P<0. 01）. However, there was no significant difference in the density of CD68+-CD163+ cells between chronic apical abscess group and periapical cyst group （P>0. 05）. （3） The ratio of CD68+-iNOS+/CD68+-CD163+ was significantly higher in chronic apical abscess group compared with periapical cyst group （P<0. 01）, while there was no significant difference in this ratio between chronic apical abscess group and healthy control group（ P>0. 05）. CONCLUSION: The expression ratio of CD68+- iNOS+/CD68+-CD163+ between chronic apical abscess and periapical cyst groups differed. Modulating the proportion of CD68+-iNOS+/CD68+-CD163+ may be involved in the development of CAP at various stages. [ABSTRACT FROM AUTHOR]

Porcine reproductive and respiratory syndrome is a viral infectious disease caused by Porcine reproductive and respiratory syndrome virus(PRRSV). The disease is one of the three most important diseases affecting the healthy development of pig industry in China. At present, the prevention and control of the disease mainly depends on the internal and external biosecurity system of the farm, taking reasonable measures to reduce the pollution of the virus, and cutting off the circulation and transmission of the virus among pigs. From the perspective of protecting susceptible animals, breeding for disease resistance is also one of the major strategies for disease prevention and control. In recent years, with the development and maturity of gene editing technology, molecular breeding, as the core technology of pig disease resistance breeding, has shown its unique advantages. Several teams have made many breakthroughs in PRRS resistance breeding by using molecular breeding technology. This article takes the PRRSV receptor as the entry point, and reviews the current research status of pig PRRS‑ resistant breeding, aiming to provide reference and clues for the prevention and control of PRRS and the research of pig disease‑resistant breeding. [ABSTRACT FROM AUTHOR]

Objective To investigate the expression of CD163 in children with biliary atresia (BA) and its relationship with liver fibrosis. Methods A total of 30 children with BA (the BA group, 14 patients with liver fibrosis grade Ⅰ-Ⅱ, 16 patients with liver fibrosis grade Ⅲ-Ⅳ) and 10 children with choledochal cyst (the CC group) were collected. Samples of liver tissue and blood were obtained from the 2 groups. The expression levels of CD163 in liver tissue and blood of the two groups were detected by immunohistochemistry and flow cytometry. The expression of sCD163 was detected by enzyme-linked immunosorbent assay (ELISA), and the value of sCD163 in evaluating BA liver fibrosis was analyzed by receiver operating characteristic curve (ROC). Results Immunohistochemical staining and flow cytometry indicated that CD163 expression levels in liver tissue and blood were significantly higher in the BA group than those in the CC group (P＜0.01), and which increased with the increased severity of liver fibrosis (P＜0.01). The expression of sCD163 also showed the same change. sCD163 predicted BA with an area under the ROC curve of 0.927, a best threshold value of 8.323 µg/L, a sensitivity of 86.67% and a specificity of 100%. Conclusion The expression of CD163 is up-regulated in children with BA, which accelerates the process of liver fibrosis. sCD163 can accurately reflect the severity of liver fibrosis in children with BA. [ABSTRACT FROM AUTHOR]

Copyright of Journal of China Medical University is the property of Journal of China Medical University Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Objective To observe the changes in M2 macrophages by detecting the changes in M2-type macrophagespecific markers and gene expression levels in mice infected with alveolar echinococcosis. Methods Forty-eight female BALB/c mice were randomly divided into the control group and model group. In the model group, we used the vesicles in the BALB/c body to prepare a suspension for injection, and the infection model of the alveolar echinococcosis was completed by intraperitoneal injection. The control group was only injected with normal saline. Eight mice were sacrificed respectively at 90, 180 and 300 d after infection, and the liver was removed. One part was fixed in formaldehyde solution for HE and immunohistochemical staining. The other part was frozen and the relative value of Fizzl mRNA was detected by qRTPCR. Results Compared with the control group, the expression of M2 macrophages’ specific marker CD163 increased significantly at 90 d, reached a peak at 180 d, and then decreased, but it was still high(P<0.05). Compared with the control group, the overall trend of M2 macrophages’ specific gene Fizz1 mRNA expression was similar to that of its specific marker CD163(P<0.05). Conclusion The M2 macrophages in the liver of the mice infected with alveolar echinococcosis increase in the middle and late stages. [ABSTRACT FROM AUTHOR]

Copyright of Journal of Henan Agricultural Sciences is the property of Editorial Board of Journal of Henan Agricultural Sciences and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

As main recipient cells for porcine reproductive and respiratory syndrome virus (PRRSV), porcine alveolar macrophage (PAM) are involved in the progress of several highly pathogenic virus infections. However, due to the fact that the PAM cells can only be obtained from primary tissues, research on PAM-based virus-host interactions remains challenging. The improvement of induced pluripotent stem cells (iPSCs) technology provides a new strategy to develop IPSCs-derived PAM cells. Since the CD163 is a macrophage-specific marker and a validated receptor essential for PRRSV infection, generation of stable porcine induced pluripotent stem cells lines containing CD163 reporter system play important roles in the investigation of IPSCs-PAM transition and PAM-based virus-host interaction. Based on the CRISPR/Cas9- mediated gene editing system, we designed a sgRNA targeting CD163 locus and constructed the corresponding donor vectors. To test whether this reporter system has the expected function, the reporter system was introduced into primary PAM cells to detect the expression of RFP. To validate the low effect on stem cell pluripotency, we generated porcine iPSC lines containing CD163 reporter and assessed the pluripotency through multiple assays such as alkaline phosphatase staining, immunofluorescent staining, and EdU staining. The red-fluorescent protein (RFP) expression was detected in CD163-edited PAM cells, suggesting that our reporter system indeed has the ability to reflect the expression of gene CD163 . Compared with wild-type (WT) iPSCs, the CD163 reporter-iPSCs display similar pluripotency-associated transcription factors expression. Besides, cells with the reporter system showed consistent cell morphology and proliferation ability as compared to WT iPSCs, indicating that the edited-cells have no effect on stem cell pluripotency. In conclusion, we generated porcine iPSCs that contain a CD163 reporter system. Our results demonstrated that this reporter system was functional and safe. This study provides a platform to investigate the iPS-PAM development and virus-host interaction in PAM cells.

Objective: To investigate serum levels and mRNA expressions of tumor necrosis factor-like weak inducer of apoptosis (TWEAK) and its receptor CD163 from the patients with psoriasis vulgaris (PV)., Methods: Peripheral blood samples were obtained from 28 patients with PV and 15 healthy control subjects. Serum levels of TWEAK and CD163 were detected by ELISA, mRNA expressions of TWEAK and CD163 in peripheral blood were analyzed by real time-PCR, and protein expressions of TWEAK and CD163 were determined by flow cytometry., Results: All the 28 PV patients were in progressive stage at the beginning of this study, 10 patients then recovered in convalescent stage after treatment. Compared to healthy controls, PV patients had higher serum TWEAK levels and lower serum CD163 levels. Serum TWEAK level in progressive stage was significantly higher than that in convalescent stage. Serum CD163 level were elevated significantly in convalescent stage compared with those in progressive stage. TWEAK mRNA expression in PV patients were significantly lower than that in healthy controls, but there was no significant differences of CD163 mRNA expression. TWEAK expression in monocytes in progressive stage and convalescent stage were significantly higher than that of controls, CD163 expression in monocytes in progressive stage and convalescent stage significantly lower than that in controls. No correlations wene found between psoriasis area and severity index (PASI) score and expression of TWEAK and CD163., Conclusion: TWEAK/CD163 pathway may play a role in PV., (Copyright© by Editorial Board of Journal of Sichuan University (Medical Science Edition).)

Objective: To investigate the correlation between tumor-associated macrophages (TAMs) and the development of high risk human papilloma virus (hr-HPV)-related cervical cancer., Methods: A total of 112 cases of cervical tissue were collected, including 16 normal cervical tissues, 55 cervical intraepithelial neoplasia (CIN) tissues and 41 squamous cervical cancer (SCC) tissues. The expression of CD163 + macrophages in the cervical tissues was detected by immunohistochemical method, and the results were analyzed in relation with the clinical data of the patients., Results: Immunohistochemical analysis showed that the cell density of CD163 + macrophages increased progressively with the increase in the tissue malignancy, in the order of normal cervical tissue, CIN Ⅰ, CIN Ⅱ-Ⅲ, and SCC. Correlation analysis revealed a positive relationship between CD163 + macrophage density and tissue malignancy ( P =0.000). The density of CD163 + macrophages was significantly upregulated in HR-HPV-positive SCC tissue ( P < 0.05). CD163 + macrophages were positively correlated with cervical lymph node metastasis ( P =0.005) and FIGO stage ( P =0.004) of SCC., Conclusions: The expression of CD163 + macrophages is positively correlated with malignant transformation of cervical tissues, and hr-HPV infection is significantly correlated with CD163 expression level in the macrophages. CD163 + macrophages can be used as predictors of the occurrence and progression of cervical cancer caused by hr-HPV infection.