[Objective] The objective of this paper was to study the effect of altitude on the contents and antioxidant capacity of polyphenols, proanthocyanidins, and flavonoids extracted from Picea koraiensis pine cones on Changbai Mountain in Yichun, and to provide theoretical basis for breeding, development and utilization of Picea koraiensis.[Method] The antioxidative indexes(i.e. total reducing capacity and DPPH· scavenging ability) and the contents of polyphenols, proanthocyanidins, and flavonoids in Picea koraiensis pine cones at different altitude(120 m, 520 m, and 920 m) that collected from the northern and the southern slopes of Changbai Mountain were determined. Particularly, the one-way ANOVA and correlation analysis were conducted by SPSS 21.0 to explore the effect of altitude on flavonoids contents and antioxidant capacity. [Results] The results showed that the contents(920 m > 520 m > 120 m) of polyphenols, proanthocyanidins, and flavonoids increased with the increase of altitude. Specifically, the correction between polyphenol content and the altitude was highly significant(P < 0.01), and that between proanthocyanidins content and the altitude was significant(P < 0.05), while there was no correction between flavonoids content and the altitude(P > 0.05). In terms of total reducing capacity and DPPH· scavenging ability, they also increased with altitude, in which the total reducing capacity was significantly correlated with the altitude(P < 0.5), while the DPPH· scavenging ability was not(P > 0.05). [Conclusion] The contents of polyphenols, proanthocyanidins and flavonoids in Picea koraiensis cones that collected in Yichun were significantly increased with the altitude increasing, this result indicated that the altitude was the dominated factor that affected the contents of these three bioactive compounds. And we can speculate that the differences in contents were resulted from the temperature change and sunlight spectrum at different altitude levels. More precisely, the higher the altitude is, the lower the temperature is, and the higher the UV-B is, and consequently, more secondary metabolites are produced. [ABSTRACT FROM AUTHOR]