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[Objectives] To understand the characteristic and the function of Malnc2310,the cis-acting elements on the promoter, the response to Fusarium crude toxin and the potential binding factors were investigated through genetic methods. [Methods]The response of promoter P1(1625 bp) and three complement fragments including P2(400 bp), P3(800 bp) and P4(425 bp), to high salinity and fusarium crude toxin were investigated through the Arabidopsis transformation. The potential binding factors on the promoter P1 and the core binding domain were studied through yeast one-hybrid screening system. [Results]The promoter of Malnc2310 contained many cis-elements, mainly including constitutive regulatory elements, phytohormone response elements, pathogen and salicylic regulatory elements, and light responsive elements through online database analysis. The average activities of GUS in all promoter fragments were higher under the Fusarium crude toxin than high salinity treatment. Moreover, the activities of GUS under P3 were similar with those under P1 during different treatments. One zinc finger protein with two C2H2 domains called ZFP-WIP2 was verified to bind to P1 and P3 through one-hybridization system. [Conclusions]This research verifies the promoter of Malnc2310 is more responsive to Fusarium crude toxin than high salinity, and one zinc finger protein ZFP-WIP2 can bind to the promoter core domain (425-1 225 bp). [ABSTRACT FROM AUTHOR]