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By employing a site-specific mutagenesis strategy for glycerol kinase (encoding by glpK), the active-site residue Met270 was replaced with He in the Bacillus subtilis genome. A mutant strain Bacillus subtilis M270I was constructed successfully by using the markerless gene replacement system. The results of growth analysis indicated that the mutant strain got a higher level of glycerol utilization. Compared with the control strain Bacillus subtilis 168 Δupp, the mutant strain exhibited higher specific growth rate (increased by 11%) and higher biomass yield (improved by 16%) with a shorten lag phase (shorten 2~4 h) in the M9 glycerol minimal medium. [ABSTRACT FROM AUTHOR]

Objective: To review the research progress of different ways of stem cells generation and cells dedifferentiation induced by reversine., Methods: The papers related to reversine inducing cells dedifferentiation and stem cells generation were reviewed., Results: To obtain stem cells, there are always some disadvantages via somatic cell nuclear transfer or gene transfection. However reversine, a small molecule, can induce cells dedifferentiation which has unique advantage. But its mechanism is still unclear., Conclusion: The chemical approach for generation of induced pluripotent stem cells by reversine may take the place of other methods.