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ObjectiveA method involving derivatization combined with liquid chromatography-tandem mass spectrometry was developed for the determination of vitamin D in infant formula food.MethodsThe samples were treated with enzymolysis， saponification， liquid-liquid extraction， and 4-phenyl-1，2，4-triazoline-3，5-dion derivatization. Chromatographic separation was performed using an Atlantis® T3 column， and gradient elution was performed using the methanol-5 mmol/L ammonium format solution as the mobile phase. The method applied positive electrospray ionization and multiple reaction monitoring mode and was quantified with the isotope internal standard method.ResultsA good linearity was obtained with a correlation coefficient above 0.99. The average recovery levels from three different ranged from 92.3% to 94.2%， and the relative standard deviations were between 3.03% and 5.34% （n=6）. The limit of quantification was 1 μg/kg.ConclusionThis method is simple， accurate with high sensitivity and high selectivity， and suitable for the determination of vitamin D in infant formula food.

BackgroundThe primary heat exchanger (PHX) used in the 10 MWt Molten Salt Reactor Experiment (MSRE) at Oak Ridge National Laboratory (ORNL), is a U-tube heat exchanger, where the shell side (hot side) contains the fuel salt from the primary loop and the tube side (cold side) carries the coolant salt from the secondary loop.PurposeThis study aims to deepen the understanding and mastery of the operational characteristics of molten salt heat exchangers, and to accumulate experience in their design and operation within molten salt reactors.MethodsFirstly, based on the design parameters, the MSRE-PHX was modeled, and theoretical calculations for shell and tube hear exchanger were conducted using the Kern method and the Bell-Delaware method. Then, software simulations were performed using HTRI Xchanger Suite, and computational fluid dynamics (CFD) simulations were also carried out with Ansys Fluent. Finally, critical performance metrics, such as the heat transfer coefficient, the pressure drop, and the heat transfer power, were obtained and compared to the MSRE operation data.ResultsThe comparison results indicate that the discrepancies from theoretical calculations, HTRI software, and CFD simulations, are all within acceptable margins to the experimental data. Notably, the greatest variance is found with the Kern method, which showed a deviation in heat transfer quantity of about 15%, while the smallest discrepancy is observed in the overall heat transfer coefficient calculated using HTRI software, differing by merely 0.16% from the experimental data.ConclusionsAll of the methods are suitable and applicable for designing and studying a molten salt shell and tube heat exchanger. Moreover, the CFD simulation can provide fine localized details of the heat transfer and flow of the molten salt fluid. This offers substantial theoretical support and practical guidance for the future design and improvement of molten salt heat exchangers.

Objective To investigate the role and mechanism of fibrinogen-like protein 2 (Fgl2) in acute kidney injury induced by rhabdomyolysis (RM-AKI). Methods Wild-type mice (WT) and Fgl2 gene knockout mice (Fgl2 KO) with the same background, were randomly divided into 4 groups respectively, that is WT+saline, WT+Gly, Fgl2 KO+saline, Fgl2 KO+Gly (n=5). The mice of the RM-AKI group were given 50% glycerol (10 μL/g) and those of the control groups were given same amount of normal saline into the hind leg by intramuscular injection. In 48 h later, the serum samples were collected to detect the levels of Scr and BUN. HE staining was employed to assess and score the severity of renal injury. qRT-PCR was used to detect the mRNA levels of Fgl2, IL-6, MCP-1, TNF-α, IL-1β and IL-10. The expression of Fgl2 and F4/80 was observed with immunohistochemical staining (IHC). TUNEL assay and Western blotting were applied to measure cell apoptosis and expression of apoptosis-related proteins, respectively. Results Fgl2 expression in renal tissue was significantly elevated in glycerol-treated WT mice compared with saline-treated WT mice. When compared with WT mice, Fgl2 deficiency significantly alleviated renal dysfunction and renal histopathological injury, with statistically lower renal injury score (P < 0.05). Moreover, the expression of pro-inflammatory factors, IL-6, MCP-1, TNF-α and IL-1β in renal tissues was decreased, while that of anti-inflammatory factor IL-10 was increased (P < 0.05). The infiltration of macrophages and apoptosis of renal cells were reduced in the Fgl2 KO mice than the WT mice. The expression of apoptosis-related proteins, Bax, cleaved Caspase-3, and cleaved Caspase-9 was reduced, while that of Bcl2 showed no significant difference. Conclusion Knockout of Fgl2 inhibits inflammatory response and reduces apoptosis in renal cells, and exerts a protective effects on RM-AKI mice.

Evaluating cardiac function from magnetic resonance images requires segmentation of the right ventricle (RV). The traditional segmentation methods, however, are often ineffective due to the complex structure of RV. Herein, a dense and multi-scale U-net (DMU-net) method is proposed to segment RV. Fifty six datasets were preprocessed, and the steps included normalization, enhancement and region of interest (ROI) extraction. Then a DMU-net was constructed by combining multi-scale aggregation with nested dense connection. The preprocessed datasets were then used to train and verify the DMU-net for predicting the results in 15 datasets with ROI extraction only. The mean of Dice index and Hausdorff distance obtained with the proposed method were 0.862 and 4.44 mm, respectively, which were better than those obtained with the conventional methods. Correlation coefficients of the endo-diastolic volume, endo-systolic volume, ejection fraction and stroke volume estimated were 0.992, 0.960, 0.987 and 0.982, respectively. The results demonstrated that the segmentation method based on the DMU-net has better relevance and consistency compared to manual segmentation, providing a promising tool the diagnosis of cardiac diseases.

Right ventricle (RV) segmentation is essential for assessing cardiac function in patients with pulmonary hypertension, tetralogy of Fallot, and so on. However, it remains difficult due to the complex structure of heart, thin myocardium and large variability of the RV, as well as the interferences from the fat nearby. Due to its high temporal and spatial resolution, cardiac cine magnetic resonance imaging is widely used for functional evaluation of the heart. This article reviews the commonly-used methods for RV segmentation from the cardiac cine magnetic resonance images. The traditional algorithms are described first, followed by the novel multi-atlas and deep learning methods. Lastly, the evaluation standards for RV segmentation are introduced. It is concluded that the deep learning-based segmentation methods may have the potential to become the method of choice in clinical settings due to their high efficiency and accuracy in the diagnosis and prognosis of the heart-related diseases.

In view of problems of expensive hardware and higher maintenance cost in standard hot standby scheme of coal mine monitoring and control system, the paper designed a hot standby system with pure software mode. The system uses combinations of file differential backup and MYSQL incremental backup to store data in main and standby computer, that is, using RSYNC file synchronization algorithm to backup file, using Binary Log mechanism of MYSQL database to open a log file, exporting modified data in a period to incremental backup file and sending incremental backup files to standby computer, and the standby computer introduces the data into database, so as to achieve synchronization operation of database. In the same way, modified data in the standby computer was synchronized to the main computer when the main computer has fault. The hot standby system has been applied in coal enterprises and meets with requirements of real-time and completeness of the data in coal mine momitoring and control system.

The study aimed to investigate the effects of modified atmosphere package combined with low-temperature storage on the chilling injury of atemoya and analyze the relationship between the physiochemical indexes and chilling stress by machine learning. The fruits were stored at room temperature (RT, 25 °C), low temperature (LT, 10 °C) and low temperature combined with modified atmosphere package (CA, 10 °C), and quality indicators such as flesh firmness, chilling injury index (CI), total phenols, relative electrolyte conductivity (EC), and phenylalanineammonialyas (PAL) activity were measured within 7 days. The results indicated that low-temperature storage effectively delayed the ripening of atemoya, retarding the decrease of flesh firmness. But atemoya stored at low temperature suffered from chilling stress, emerging soaking spots, EC increased and the content of MDA in the flesh accumulated. On this basis, several machine learning algorithms were established and the optimal model chosen for predicting CI was Ridge regression. Explanation analysis (SHapley Additive exPlanations, SHAP) showed that storage time, soluble solids, water loss rate, soluble proteins, and total phenols contributed significantly to the model and were closely related to chilling stress. Low temperature combined with modified atmosphere could increase the content of soluble substances and phenols, enhance the osmoregulation capacity and clearing-reactive-oxygen ability, and thus maintain the integrity of membrane and mitigate the chilling injury. [ABSTRACT FROM AUTHOR]

In this study, buffalo cheese was prepared with rennet from Moringa oleifera seeds. The process was optimized based on sensory characteristics and hydrolysis degree. The composition of water-soluble peptides from the cheese was analyzed. Furthermore, bioactive peptides with angiotensin converting enzyme (ACE) inhibitory activity were identified and their potential molecular mechanisms of action were explored. The optimal conditions determined were as follows: pH 5.56, rennet dose 0.14%, incubation time 32 min, and stretching temperature 80 ℃. Under these conditions, the hydrolysis degree of cheese was 12.56% with a uniform and elastic texture, a soft and delicate taste, a uniform and glossy color, and a rich aroma. By liquid chromatography-tandem mass spectrometry (LC-MS/MS), 1 600 peptides with molecular mass less than 3 kDa were detected in the cheese, 613 of which demonstrated good bioactivity, predominately derived from β-casein and αS1-casein. Notably, 37.29% of the peptides exhibited ACE inhibitory activity. By comparing with the bioactive peptide database and using bioinformatics, four peptides proven to have ACE inhibitory activity and four novel peptides with potential ACE inhibitory activity were identified. Molecular docking showed that the four novel peptides could effectively bind to ACE. Among them, FGGL and FSPL bound tightly to the S2 active pocket, thus exhibiting strong ACE inhibitory activity. This study provides a scientific basis for the development and utilization of new plant rennet resources and characteristic buffalo cheese.

Abstract [Objective] Most intersectional hybrids in Paeonia are triploids and generally sterile, producing a small number of hybrid offspring. Observing the meiotic chromosome behaviors of ‘Bartzella’, an intersectional hybrid with high ornamental value and several hybrid offspring, can provide insight into the formation mechanism of its low fertility. [Methods] The study utilized the anthers of ‘Bartzella’ as materials to observe the meiotic chromosome behaviors of pollen mother cells (PMCs) through the squash method. [Results] (1) In metaphase Ⅰ, a significant number of univalents were formed in ‘Bartzella’ PMCs. In anaphase Ⅰ, various types of chromosomal aneuploid segregation were observed, accounting for up to 89.13%. (2) During the first meiotic division, chromosome bridges and fragments were found in 27.20% of the PMCs; during the second meiotic division, this percentage was increased to 47.68%. (3) In metaphase Ⅱ, spindle positioning abnormalities were relatively common, with fused spindles accounting for 27.06% and tripolar spindles accounting for 12.84%. The meiotic products included up to 72.41% of dyads and 13.43% of triads, which could generate unreduced gametes accounting for about 67%. [Conclusion] Chromosome aneuploidy segregation and loss of chromosome segments during meiosis may be the primary causes of the high sterility of ‘Bartzella’. Fused spindles, tripolar spindles, and abnormal cytokinesis at the end of second meiotic division may result in the production of unreduced gametes. The significant number of unreduced gametes might give ‘Bartzella’ the potential polyploid breeding. However, interploidy hybridization barriers could restrict its hybrid affinity.

Objective The microbial dolomite of the second Member of the Dengying Formation（Deng 2 Member） in the Penglai area of the Sichuan Basin is widely distributed and considered a potential target for deep carbonate oil and gas exploration. Unlike conventional high-quality reservoirs characterized by porosity and karst fractures, the carbonate rocks of the Deng 2 Member mainly consist of microbial dolomite. The genesis and diagenetic evolution of these high-quality reservoirs remain unclear. Methods This study employed petrographic thin sections, scanning electron microscopy (SEM), cathodoluminescence, and computed tomography （CT）scanning to conduct a detailed analysis of the microbial dolomite reservoirs in the Dengying Formation, using field outcrop and core samples. The aim was to gain a deeper understanding of the effects of diagenesis on pore formation and the development of high-quality reservoirs. Results and Conclusion The microbial carbonate reservoirs in the study area were characterized by low porosity and very low permeability, predominantly consisting of fracture-porosity (cavity) type microbial dolomite. The reservoir space was primarily composed of intergranular dissolution pores, residual framework dissolution pores, intragranular dissolution pores, and small to medium-sized dissolution cavities, with minor occurrences of intercrystalline pores and intercrystalline dissolution pores. The Deng 2 Member has undergone multiple diagenetic processes; penecontemporaneous dissolution and early epigenetic dissolution were key factors in enhancing porosity. Significance These findings enhance the understanding of the genesis of high-quality microbial carbonate reservoirs in the Ediacaran System of the Sichuan Basin and provide valuable information for deep oil and gas exploration and development in the region.

Urbanization in China has entered a new phase that emphasizes both scale expansion and quality improvement. This has led to demands on urban functional structures and rational urban planning. Street space serves as a vital spatial carrier for meeting urban residents' needs, such as travel, shopping, and leisure. It is comprised of urban roads and their ancillary facilities, buildings along the route, and many other elements. However, existing studies typically focus on the traffic function of urban roads, overlooking other functional aspects of street space as complex public activity areas, thereby hindering the optimization of street space quality. Therefore, there is a need to propose a classification method for the urban street space functions. Given the proliferation of taxi trajectory data and street view imagery, street space can be described in detail from the citizens' perspectives. Therefore, this study proposes a street space classification method that integrates taxi trajectory and street view imagery to delineate urban functions. The dynamic travel characteristics of urban residents in the street space are constructed using the taxi trajectory, including the number of trajectories passing by the street, the number of origin points on the street, and the number of destination points on the street. The physical environment characteristics of the street are constructed using the street view image, which contains single element street features, combined element street features, and overall element street features. Subsequently, based on residents' dynamic travel characteristics and the physical environmental characteristics of street space, the K-Means method is utilized to allocate street spaces with similar urban functions into the same clusters. Taking Bao'an District of Shenzhen as a case study, it was found that clustering with K=3 yielded the most interpretable results. Subsequently, based on street characteristics and auxiliary POI information (including POI density and POI enrichment index), street spaces were successfully classified into three urban functional types: commercial, traffic, and residential. Further analysis was conducted by integrating street view images, the Gaode map, and the 24-hours distribution of the trajectory, which validated the reliability of the classification results, achieving an experimental accuracy of 81%. Additionally, this study constructed two sets of ablation experiments for a comparative analysis of the effectiveness of the characteristics extracted in this study. The results of street space function classification based on taxi trajectory data and street view image data showed classification accuracies of 67% and 34%, respectively, which are lower than the classification effect of combining the two data sources. The results of street space classification based on feature selection from taxi trajectory data indicated that the classification accuracy considering only the weekday taxi trajectory features was 69%, and considering only the taxi trajectory features during T08:00-22:00 on both weekdays and weekends was 73%, which is lower than the classification effect of using three types of trajectory features throughout the entire day. By integrating the characteristics extracted from multiple data sources, the methods took into account both pedestrian and vehicle traffic functions, as well as the diversity of the streetscape environment and residents' activities. The identification results can provide references for the design and quality optimization of street space, as well as detailed investigations into urban functional areas such as residential, commercial, and traffic street space.

Gear surface damage is an important factor affecting gear transmission. It is extremely important to improve the efficiency and accuracy of gear surface damage identification. ResNet recognition model of gear surface damage is established based on Pytorch architecture, dataset is expanded by means of data enhancement, model training is optimized by means of transfer learning, and four ResNet structures are compared. The results show that the dataset composed of 640 images after the enhancement of 64 original image is not enough to meet the needs of model training for a large amount of data; using transfer learning can improve the speed and accuracy of model training, and meet the requirements of gear surface damage identification; the ResNet-101 model is the optimal structure in this framework. This research has important scientific significance and engineering value for the recognition of gear surface damage.

[Objectives] To clarify the influence of sex, age (days) and temperature, on the flight ability of adult Hyphantria cunea, and there by provide information essential for monitoring, and predicting, the dispersal of this pest. [Methods] The effect of gender, age and temperature on the flight ability of adult H. cunea was measured in an insect flight mill. [Results] Males were significantly stronger fliers than females with a maximum flight distance of 17.87 km and cumulative flight time of 17.38 h. One day-old males had the longest cumulative flight distance; the average cumulative flight distance and average cumulative flight time of males decreased with age. The maximum female flight distance was 8.21 km and the longest cumulative flight time was 7.4 h. Female flight distance tended to first increase, then decrease, with age. Three day-old females had the maximum cumulative flight distance. A two-way ANOVA of the average cumulative flight distance, average cumulative flight time, and average flight speed, did not detect a significant interaction between sex and age. Male flight speed was significantly slower at a temperature of 16 °C. [Conclusion] Male H. cunea are stronger fliers than females. Male flight ability gradually decreases with age, whereas that of females tends to increase up to 3 days of age, then decrease. Low temperatures reduce the flight speed of H. cunea males. [ABSTRACT FROM AUTHOR]

In this study, angiotensin-converting enzyme (ACE) inhibitory peptides from an enzymatic hydrolysate of Moringa oleifera seeds were separated by sequential ultrafiltration and ion exchange chromatography. The peptide sequences were identified by high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) and potential ACE inhibitory peptides were selected by bioinformatics and molecular docking; their secondary structure was analyzed by Fourier transform infrared (FTIR) spectroscopy and their in vitro activity was evaluated by enzymatic inhibition kinetics and the methyl thiazolyl tetrazolium (MTT) method. The results showed that peptide fraction F-b had a good antihypertensive effect. A total of 11 peptide sequences were identified. Peptide QGPRPQ was identified as a potential ACE inhibitory peptide with a half-maximum inhibitory concentration (IC50 ) (1.15 ± 0.3) mmol/L. Molecular docking showed that QGPRPQ could better bind to ACE through hydrogen bond and hydrophobic interaction. Secondary structure analysis showed that QGPRPQ was composed of 22.8% α-helix, 33.3% β-fold and 43.9% β-turn. The mode of inhibition of QGPRPQ was mixed type, and it had no toxic effect on HepG2 cells at a concentration lower than 0.01 mg/mL. This study can provide an important theoretical basis for the development and utilization of hypotensive peptides derived from M. oleifera seed protein.

Objective·To analyze the progression of children with severe coronary artery lesions due to Kawasaki disease by coronary artery angiography, and evaluate the diagnostic value of echocardiography in these children.Methods·A retrospective analysis was performed to enroll children with Kawasaki disease whose coronary artery lesions were graded Ⅳ or above from Shanghai Children's Medical Center, Shanghai Jiao Tong University School of Medicine, from January 2013 to January 2023. The subjects were required to have received at least 2 times of coronary angiogram, and their clinical and imaging data were collected to analyze the progression of the lesions. Echocardiography results were compared with the results of the coronary angiogram.Results·A total of 21 children were included, including 15 males and 6 females, with a median age at onset of 3 years and 6 months, a median age at initial coronary angiography of 7 years and 11 months, a median interval of 4 years and 5 months between the time of onset and initial angiography, a median age at angiographic review of 9 years and 2 months, and a median interval of 1 year and 3 months between the time of initial angiography and review. Coronary stenosis or occlusion was detected in 13 children in the initial angiography, of whom 6 underwent coronary artery bypass grafting (CABG) and had their angiography reviews 1 year later. The review results showed that the bridging vessels were unobstructed and no obvious stenosis was observed. Fifteen children had progression of the lesions detected by echocardiography in the subsequent follow-up and had their angiogram reviews, of whom 8 had significant progression of the coronary lesions. Intracoronary balloon dilatation was performed in 1 case, and CABG was performed in another case. Sixteen lesions of coronary stenosis or occlusion were detected in the initial angiography in 21 children, while only 1 lesion of coronary stenosis was detected by echocardiography during the same period of time. Twenty-eight medium- to large-sized coronary aneurysms were detected in the initial angiography in the 21 children, and the diameters of the 28 aneurysms measured by echocardiography and coronary angiogram were subjected to the Bland-Altman analysis. The Bland-Altman analysis showed that the difference in maximum diameter between 2 methods was (1.63±2.33) mm, with 95%CI of -2.95‒6.21 mm.Conclusion·Coronary artery lesions due to Kawasaki disease may be progressive; in the children with severe lesions, coronary artery stenosis or occlusion may be missed or misdiagnosed and some errors may exist in the measurement of diameters of aneurysms by echocardiography. Regular review of coronary angiography is needed.

Provincial-level natural resources survey and monitoring play a crucial role in the national natural resources survey and monitoring system. Constructing a provincial-level system is essential for effectively implementing the national survey and monitoring work. However, the construction of such a system in Guangdong Province, a typical tropical and subtropical coastal region with diverse natural resources and distinctive geographical characteristics, presents significant challenges. This study focuses on addressing several problems encountered in Guangdong Province, including the lack of optical image data due to the region's cloudy and rainy climate, fragmented monitoring patches resulting from complex terrain and landforms, rapid changes in the surface caused by high-intensity human activities, and the difficulty in managing a wide range of monitoring objects. By clarifying the goals of natural resources survey and monitoring, this study systematically reviews the status and practical needs of a provincial-level survey and monitoring work and summarizes the key issues in the construction of the survey and monitoring system. This study proposes an overall construction plan for a provincial-level system that includes establishing a unified basic platform, which aids in implementing a fast and coordinated monitoring mode, developing flexible and efficient application services, and strengthening intensive and refined support environments. It also analyzes critical challenges, such as organizational model restructuring, accurate object recognition, and technological integration. Finally, this study presents a path to achieve the provincial-level natural resources survey and monitoring, focusing on object-based businesses, information implementation, technological intelligence, targeted evaluation, and service knowledge. Regarding investigation targets, this study emphasizes adopting a "business-oriented" approach, enhancing the alignment of investigation and monitoring with precise business strategies and facilitating bidirectional feedback. Regarding organizational implementation, this study emphasizes strengthening support through "information technology," establishing provincial-level coordination and a municipal and county-level division of labor model, commonly referred to as the "general-subordinate-general" organizational structure. Regarding monitoring technology, this study emphasizes adopting an "intelligence-led" approach; it is primarily centering on pivotal technological research related to collaborative perception, intelligent processing, integrated management, and intelligent services. Regarding analysis and evaluation, this study emphasizes a "target-oriented" approach, centered around supporting economic and social development and optimizing natural resource management through comprehensive analysis and evaluation. Regarding application services, this study emphasizes delivering "knowledge-based" services and exploring the development of a model that encompasses the knowledge ontology of natural resource investigation and monitoring. This study promotes the orderly construction of a survey and monitoring system in Guangdong Province, provides a scientific basis for provincial-level natural resource surveys and monitoring work and serves as a reference for the construction of survey and monitoring systems in other regions.

The objective of this study is to explore the effect of soil moisture on the formation of storage roots in sweet potato and its relationship with the number and uniformity of storage roots. In this experiment, the sweet potato variety Yanshu 25 was used as the experimental material. We performed five soil relative water content levels with 50%±5% (W50), 60%±5% (W60), 70%±5% (W70), 80%±5% (W80), and 90%±5% (W90) at root branching stage. The effects of soil moisture on the content of carbohydrate and the activity of metabolic enzymes in the potential storage roots, as well as the number, uniformity and yield of storage roots at the harvest time were studied by a two-year field experiment. The results showed that the content of sucrose, glucose, and fructose and the activity of sucrose invertase in the potential storage roots of sweet potato in W70 treatment were significantly higher than those of other treatments during 10-35 days after planting. The activity of sucrose synthase and the content of starch in the potential storage roots of sweet potato in W70 treatment were significantly lower than those of other treatments at 10-25 days after planting, while the trend was opposite during 30-35 days after planting. Compared with other treatments, the number of storage roots per plant and the yield of storage root in W70 treatment increased by 14%-120% and 22%-122%, respectively. The variable coefficient of the single storage root weight decreased by 9%-34%. The soil relative water content of 70% at root branching stage of sweet potato was beneficial to the formation of storage roots, which increased the number of storage roots per plant, weight of the single storage root and yield of storage roots, and improved the commercial quality of storage roots at harvest time. [ABSTRACT FROM AUTHOR]

ObjectiveThe aim of this study was to evaluate the serotype， drug resistance， and molecular typing characteristics of Salmonella isolated from patients with foodborne diseases in Guizhou Province in 2021.MethodsA total of 164 Salmonella strains were isolated from patients with diarrhea under the Guizhou Provincial Active Surveillance of Foodborne Diseases program in 2021. These strains were subjected to slide agglutination serotyping test， minimum inhibitory concentration tests with 14 antibiotics， and pulsed-field gel electrophoresis （PFGE） for molecular typing.ResultsThe 164 Salmonella strains were divided into 25 serotypes. The dominant serotypes were S. typhimurium （46.34%，76/164）， S. enteritidis （15.24%，25/164）， and S. javana （9.15%，15/164）. The drug resistance rate of the 164 Salmonella strains was 100% （164/164）. The multidrug resistance rate was 86.59% （142/164）. Out of the 15 antibiotics tested， resistance was most frequently observed against ampicillin （95.12%，156/164）， tetracycline （78.05%，128/164）， and nalidixic acid （63.41%，104/164）. Seventy-two S. typhimurium， 24 S. enteritidis， and 15 S. javana strains were divided into 58， 12， and 3 different PFGE clusters， respectively.ConclusionThere were many Salmonella serotypes detected in patients with diarrhea in the Guizhou Province， and multidrug resistance was common. PFGE fingerprints demonstrated genetic diversity of the isolated strains. Monitoring of drug resistance in Salmonella should be strengthened， especially for the clinical treatment of S. typhimurium.

In order to solve the serious dusting problem for soil thermal desorption device, the method of acoustic agglomeration combined spray was applied to the dust removal of the thermal desorption device. The effects of sound frequency, initial dust concentration, sound pressure level and spray flow rate on the dust removal efficiency (average particle size of dust less than 10 μm) were studied. The experimental results showed that under the condition of sound frequency of 1400 Hz and spray concentration of 1.0 L·m^-3, the dust removal efficiency of the agglomeration chamber reached 98% within 60 s. When the sound pressure level was increased from 145 dB to 153 dB, the dust removal efficiency is increased by 38%. SEM results showed that the particles were crushed and aggregated after acoustic wave treatment. Adding spray could improve the dust removal efficiency, but the effect of spray concentration on final dust removal efficiency was not significant. The optimal sound frequency after spraying was 1400 Hz. The higher initial concentration of dust and spray could lead to the higher dust removal rate and the final dust removal efficiency in the early stage of agglomeration. When the spray concentration was 2.0 L·m^-3, the dust removal efficiency was 44% higher than that without spray, indicating that the acoustic wave agglomeration had a better effect on the use of high concentration aerosol. The results of this paper could offer useful information in the investigation and development of dust removal technology of thermal desorption device.

【Objective】 To explore the effects of the interaction between leaf spraying and soil water on the growth yield and water use efficiency of tomato plants in greenhouse. 【Method】 The objective is through pot experiment, this study took greenhouse tomato (Jingfan 404) as the research object, four exogenous selenium (selenium source: Na2SeO3) spraying concentration (S0: clear water control, S2.5: 2.5 mg/L, S5: 5 mg/L and S10: 10 mg/L) and three irrigation control levels were set (the lower irrigation limit was controlled as 50% of field water capacity (W1: severe drought), 65% (W2: mild drought), 80% (W3: full water supply), a total of 12 treatments, to study the effects of different soil moisture conditions and foliar spraying different concentrations of selenium on the growth, selenium content, yield and water use efficiency of tomato plants and fruits in greenhouse. 【Result】 With the increase of irrigation control level, plant height, stem diameter and fruit average growth rate increased significantly, and significantly affected leaf selenium content. The effects of exogenous selenium on plant height and tomato growth rate reached a significant level (p

Aiming at the problems of slagging and high temperature corrosion of water wall in a 1 000 MW ultra-supercritical double tangential boiler, the mechanism of slagging and high temperature corrosion of boiler was analyzed by X-ray diffraction (XRD) and X-ray fluorescence (XRF).The results show that the presence of sodium and calcium in coal reduces the ash melting point of coal, causing boiler slagging. Sulfide-type high temperature corrosion is the main type of high temperature corrosion of the boiler. Through coal blending optimization and air distribution adjustment, the coal quality feeding into the furnace and the air distribution mode of the burner were optimized. The results show that selecting low-sulfur and low-slagging coal for blending can effectively alleviate the problem of slagging in the furnace. The increase of the total air volume of the boiler and the reasonable adjustment of the secondary air distribution of the burner can effectively reduce the concentration of the reducing atmosphere in the furnace, and alleviate the problem of high temperature corrosion in the furnace. The prediction results of boiler corrosion tendency by numerical simulation method show that the high-temperature corrosion should be further prevented in the hot corner area of the furnace by adding adherent wind or water wall spraying.

Golden pomelo is delicious and nutritious and has gained great popularity. Golden pomelo will generate bitter taste with the extension of storage time, which seriously affect the development of the related industry. To explore the reason behind, the present work used Meizhou's golden pomelos the research object to investigate the source of bitterness. After grading, the content of titratable acid, soluble solids content, maximum adhesion force, limonin content, nomilin content, naringin content were simultaneously determined by methods such as the random forest method. The results showed that the soluble solids content was extremely significant and positive correlated (R=0.78) with sensory scores. Nomilin (R=-0.69) content and naringin (R=-0.59) content were significantly and negatively correlated with sensory scores. The bitterness source of the golden pomelo after a long-term storage was closely related to its soluble solids content. This study can provide a certain theoretical basis for the research on debittering of golden pomelo and rapid assessment of the fruit quality. [ABSTRACT FROM AUTHOR]

Aiming at the defects of low efficiency and large computational complexity of traditional structural optimization design methods, a lightweight design method was proposed based on combined approximation model. Taking the two-stage gantry structure of a certain type of internal combustion forklift as an example, the upper and lower flanges and web thicknesses that had the greatest influences on the performance of the gantry were selected as the lightweight design variables by TOPSIS method. 100 sample points including design variables and performance responses were obtained based on OptLHD method. Combined approximation model of outer gantry mass, maximum stress, and maximum deformation were constructed, combined with quadratic sequence programming algorithm, the lightweight design was developed. And the results show that the quality of the outer gantry is reduced by 13.36% and the maximum stress Is reduced by 4.65%. [ABSTRACT FROM AUTHOR]

This paper discussed the construction of Traditional Chinese Medicine continuous nursing service system under the background of Internet Plus. The sub-professional nursing group was set up to train nursing professionals of TCM, and the TCM nursing clinic was set up, based on the "hospital-community-family" management model, the hospital-patient interactive network platform, patients' Wechat group and patients' health files were established to support the implementation of TCM continuous nursing service. The construction of TCM continuous nursing service system under the background of Internet Plus can improve the ability of nurses, provide multi-level medical service and meet the needs of patients at different levels. (本文探讨“互联网+”背景下中医延续护理服务体系的构建方法。成立相关护理亚专业学组, 培养中医护理专科人才, 开设中医护理门诊, 通过医院与患者间的交互网络平台、患者微信群, 依据“医院-社区-家庭”管理模式开展中医延续护理, 同时建立患者健康档案, 提供中医延续性护理服务。“互联网+”背景下中医延续护理服务体系的构建能够提升护理人员的服务能力, 提供多层次医疗服务, 满足不同层次患者需求。)

Objective To observe the effect of using the therapeutic apparatus for music therapy based on Traditional Chinese Medicine (TCM)syndrome differentiation in the treatment of functional abdominal distension. Methods Totally 60 patients with functional abdominal distension were randomly divided into the control group and the intervention group, with 30 cases in each group. All patients received a routine treatment and nursing (7 days per course, two courses), and the music therapy based on TCM syndrome differentiation by using a therapeutic apparatus was performed in the intervention group. The Gastrointestinal Symptom Assessment score and overall effective rate were compared between two groups. Results The Gastrointestinal Symptom Assessment score decreased in both two group after two courses of treatment, and the Gastrointestinal Symptom Assessment score in the intervention group was lower than that in the control group(P＜0. 01). The overall effective rate was 93. 33%(28/30) in the intervention group, which was significant higher than 73. 33%(22/30) in the control group(P＜0. 01). No serious adverse reaction was reported in either group. Conclusion The therapeutic apparatus for music therapy based on TCM syndrome differentiation is safe and effective in the treatment of functional abdominal distension. (目的 观察运用中医辨证移情音乐胃肠治疗仪改善功能性腹胀的效果。方法 将符合纳入标准的60例功能性腹胀患者随机分为两组, 各30例。对照组接受常规治疗和护理, 干预组在常规治疗的基础上采用中医辨证移情音乐胃肠治疗仪。两组均以7 d为1个疗程, 共2个疗程。记录与评价患者腹胀症状, 对比两组干预效果。结果 干预后, 两组胃肠症状评分均较干预前下降, 且干预组胃肠症状评分低于对照组, 差异有统计学意义(P＜0. 01)。干预组总有效率93. 33%(28/30), 高于对照组的73. 33%(22/30), 差异有统计学意义(P＜0. 05)。两组均无严重不良反应。结论 中医辨证移情音乐胃肠治疗仪改善功能性腹胀具有较好的疗效, 且安全可行。)

ObjectiveTo assess the anxiety and depression symptoms and sleep quality in patients with menstrual migraine， so as to provide references for the prevention and treatment of menstrual migraine and its comorbidity.MethodsAccording to the diagnostic criteria of the International Classification of Headache Disorder， third edition （ICHD-3）， 501 female migraine patients， including 112 patients with menstrual migraine and 389 patients with non-menstrual migraine， who attended the outpatient clinic of the Affiliated Hospital of North Sichuan Medical College from February 2019 to February 2020， were selected for the study. The general and clinical data of the patients were collected， meantime， patients were assessed using Hamilton Anxiety Scale （HAMA）， Hamilton Depression Scale-24 item （HAMD-24）， 6-item Headache Impact Test （HIT-6） and Pittsburgh Sleep Quality Index （PSQI）.ResultsThe detection rates of pure anxiety， pure depression， comorbid anxiety-depression and sleep disorders in patients with menstrual migraine were higher than those in patients with non-menstrual migraine （χ2=4.198， 4.355， 5.236， 8.624， P

【Objective】 Irrigation pit is a technology to collect and store water for it to be used in irrigation. The objective of this paper is to study experimentally the impact of burying depth of emitters of subsurface irrigation in combination with a pit on water infiltration in red soil. 【Method】 The experiment was conducted indoors using soil tanks. The moving distance of the wetting front, cumulative infiltration and spatiotemporal distribution of soil water content under different irrigation amounts from 1 to 3 L, and emitter depths from 10 to 20 cm, were measured and calculated. We then modelled soil water movement in the tanks numerically based on the Richards’ equation, and fitted the infiltration process to different models. 【Result】 The infiltration rate increased with irrigation amount and decreased as the burying depth of the emitters increased. The infiltration rate declined as the time elapsed. In the first 540 min after onset of the experiment, the wetted area and the maximum moving distance of the wetting front increased with irrigation amount, thereby driving the water moving towards the bottom-right of the tanks. The maximum moving distance of the wetting front in the vertical direction was greater than that in the horizontal direction; the influence of irrigation amount on soil water content was greater than the impact of the emitter depth. 【Conclusion】 The Kostiakov model was more accurate than the Philip model to describe the water infiltration, and the numerical model can simulate spatiotemporal dynamics of the water in the soil tanks.

The goal of relativistic heavy-ion collisions is to determine the phase boundary of quantum chromodynamics (QCD) phase transitions. Critically sensitive observables are suggested to be higher-order cumulants of conserved charges. The non-monotonous behavior of higher cumulants was observed at the relativistic heavy-ion collider (RHIC). However, it remains unclear whether these non-monotonous behaviors are critically related. We studied the influences of non-critical fluctuations, finite system size, and limited evolution time to determine if they cause non-monotonous behavior. First, we examined the minimum statistics required for measuring the fourth cumulant. The minimum statistic obtained using the centrality bin width correction (CBWC) method was 25 M. We suggest using a 0.1% centrality bin in the CBWC method instead of each Nch. With a 0.1 centrality bin width, 1 M statistics are sufficient. We then pointed out the statistical fluctuations from the limited number of final particles. By assuming the independent emission of each positive (or negative) charged particle, the statistical fluctuations of positive (or negative) charged particles were presented by a Poisson distribution, and the statistical fluctuations of net-charged particles were their evolution. The obtained statistical fluctuations for net protons, net electronic charges, and net baryons were consistent with those from the Hadron Resonance Gas model. In addition, the measured cumulants at RHIC/STAR are dominated by these Poisson-like statistical fluctuations. At the end of this section, we suggest the pooling method of mixed events and demonstrate that the sample of mixed events accurately presents the contributions of the background. Dynamic cumulants were defined as the cumulant of the original sample minus that of the mixed sample. Dynamical cumulants were shown to simultaneously reduce the influence of the statistical fluctuations, centrality bin width effects, and detector efficiency. Second, because the system is finite, the correlation length at the critical point is not developed to infinity in contrast to the system at thermal limits. Using a Monte Carlo simulation of the three-dimensional three-state Potts model, we demonstrated the fluctuations of the second- and fourth-order generalized susceptibilities near the temperatures of the external fields of the first-, second-, and crossover regions. Both the second- and fourth-order susceptibilities showed similar peak-like and oscillation-like fluctuations in the three regions. Therefore, non-monotonic fluctuations are associated with the second-order phase transition and the first-order phase and crossover in a finite-size system. The exponent of finite-size scaling (FSS) characterizes the order of transitions or crossover. To determine the parameters of the phase transition using the FSS, we studied the behavior of a fixed point in the FSS. To quantify the behavior of the fixed point, we define the width of the scaled observables of different sizes at a given temperature and scaling exponent ratio. The minimum width reveals the position of the fixed point in the plane of the temperature and scaling exponent ratio. The value of this ratio indicates the nature of the fixed point, which can be a critical, first-order phase transition line point, or crossover region point. To demonstrate the effectiveness of this method, we applied it to three typical samples produced by a three-dimensional three-state Potts model. The results show that the method is more precise and effective than conventional methods. Possible applications of the proposed method are also discussed. Finally, because of the limited evolution time, some processes in relativistic heavy-ion collisions may not reach thermal equilibrium. To estimate the influence of the nonequilibrium evolution, we used the three-dimensional Ising model with the Metropolis algorithm to study the evolution from nonequilibrium to equilibrium on the phase boundary. The order parameter exponentially approaches its equilibrium value, as suggested by the Langevin equation. The average relaxation time is defined. The relaxation time is well represented by the average relaxation time, which diverges as the zth power of the system size at a critical temperature, similar to the relaxation time in dynamical equations. During nonequilibrium evolution, the third and fourth cumulants of the order parameter could be positive or negative depending on the observation time, which is consistent with the calculations of dynamical models at the crossover side. The nonequilibrium evolution at the crossover side lasts briefly, and its influence is weaker than that at the first-order phase transition line. These qualitative features are instructive for experimentally determining the critical point and phase boundary in quantum chromodynamics.

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Objective The purpose of this study was to analyze the etiology of pulmonary infection in the advanced lung cancer after chemotherapy, and investigate the application of antimicrobial agents. Methods From March 2009 to March 2012, 102 cases of advanced lung cancer patients were treated in our hospital. Their relevant samples were collected and analyzed for etiology and antimicrobial susceptibility testing. Results 102 patients with advanced lung cancer and pulmonary infection have 211 co-culture pathogens, including 146 of gram-negative bacteria (G-) (69.2%), 51 of Gram-positive bacteria (G+) (24.2%), and 14 of fungi (6.64%) .Escherichia coli showed moderate susceptibility to cephalosporins, of which the susceptibility to cefuroxime and cefotaxime was 100%. Klebsiella pneumonia exhibited high susceptibility to gentamicin, with the sensitivity of 100%. Pseudomonas aeruginosa and Enterobacter showed high susceptibility to ciprofloxacin and gentamicin, with the sensitiuity of 65.4% and 77.3% respectively. Conclusion Selecting and using the antimicrobial agents according to the results of pathogen detection in patients, could improve the effectiveness of antibiotic treatment and reduce bacterial resistance. [ABSTRACT FROM AUTHOR]

The genetic tests of the preimplantation human embryo mainly include: preimplantation genetic diagnosis (PGD) and preimplantation genetic screening (PGS). PGD based on PCR and FISH has been used to detect the single gene disorders and inherited chromosome abnormalities for the last 20 years. PGS with the same technology in PGD was used to detect aneuploidy chromosomes in embryos, to select those embryos with normal chromosomes for IVF transfer. It has been applied in those patients of advanced age, repeated implantation failure, repeated miscarriages and severe male factor infertility. There are dramatic changes in the PGD methodology. Both array --comparative genomic hybridization and single nucleotide polymorphism arrays have been introduced into clinical practice for PGD and PGS. In this review, we introduced some new methods and controversies in the field of PGD/PGS. [ABSTRACT FROM AUTHOR]

This article summarizes the study progress of the detection technology of pesticide residues in textile. The review describles the current status of pesticide residues, the relevant detection standards, the applied detection technology, and the development tendency. About to sample pre-treatment technology, the technology of the accelerated solvent extraction ( ASE ), the solid phase micro-extraction ( SPME ) and the matrix solid phase dispersion ( MSPD ) extraction are introduced as focal points. Instrument detection technology is mainly introduced gas chromatography and hyphenated te chnique s, high performance liquid chromatography and hyphenated techniques such as analysis of pesticide residues in textile applications. Summarizes the detection of pesticide residues in textile technology trends, in order for the analysis of pesticide residues detection technology improvement and development to provide a theoretical basis. [ABSTRACT FROM AUTHOR]

In order to meet the shortest-path searching in game map, this paper presented an optimized adaptive genetic algorithm. It used the environment operator which was associated with the number of nodes and arcs of the graph to set the crossover probability and the mutation probability considering the whole population distribution and evolutionary potential. Experiments show that the algorithm can avoid falling into local optimal solutions, ensure the search success rate, and improve the search speed. It provides some valuable work in game engine designing. [ABSTRACT FROM AUTHOR]

Carbon fiber is composed of crystallite and amorphous carbon. The X-ray diffraction (XRD) and Raman spectroscopy were used to investigate the influencing mechanism of the microstructure of crystallites of polyacrylonitrile (PAN)-based carbon fibers on tensile strength. The results show that the crystallinity, the degree of graphitization, the degree of crystallites structural disorder and the crystallite size have obvious effects on tensile strength. The larger the crystallinity and the degree of graphitization,the larger the tensile strength; the smaller the degree of crystallites structural disorder, the larger the tensile strength; the larger the crystallite size, the smaller the tensile strength. Comparing T300 with T700, the increment of tensile strength, which is caused by the increase of the crystallinity and the degree of graphitization, and the decrease of the degree of crystallites structural disorder, is larger than the decrement of tensile strength, which is caused by the increase of the crystallite size. Therefore, the tensile strength of T700 is larger than that of T300, and the same with that the tensile strength of T800 is larger than that of T700. When M35J is compared with M40J, the increment of tensile strength, which is caused by the increase of the crystallinity and the degree of graphitization, and the decrease of the degree of crystallites structural disorder, is smaller than the decrement of tensile strength, which is caused by the increase of the crystallite size. Therefore, the tensile strength of M40J is smaller than that of M35J, and the same with that the tensile strength of M46J is smaller than that of M40J. In M35J, M40J and M46J, the bigger crystallite size is a crucial factor that affects the tensile strength.

Vegetation is a component of the natural river. To understand the interaction between vegetation and water flow is of scientific and practical significance for the protection of wildlife habitats, the control of water body eutrophication, the ecological restoration of rivers and lakes, and the management of riverways. This paper reviewed the researches about the interaction between vegetation and water flow in riverways, summarized the research progress in the effects of the vegetation on the resistance coefficient and water flow structure, and introduced the applications of numerical simulation in this research field. Based on the previous studies, the effects of river section shape, plant individual form, and vegetation distribution pattern on the flow regime of water flow in vegetation section were analyzed. For further study, the importance of deeply understanding the hydraulics mechanisms of the interaction between vegetation and water flow in terms of the diversity of river morphology, the vegetation variation at different spatiotemporal scales, the water flow distribution in vegetation section, and the three dimensional turbulent simulation was expatiated. [ABSTRACT FROM AUTHOR]

To improve the detection precision of the urban rail vehicle wheel key parameters, a new non-contact dynamic detection method based on opening up a "detect window" at the wheel/rail contact point is put forward. The image geometric mapping model of the wheel tread and the coordinate system for detection are established, and the calculation method of wheel key parameters is established on the basis of coordinate translation methods. According to the actual application regulations of urban rail vehicle wheels, the key parameters of optical section detecting window are selected. The wheel key parameters checking experiments are carried out by use of the developed test bed. The results show that the wheel key parameter error values obtained by the method are all less than 1%. By opening up the "detect window" at the wheeL/rail contact point, the light source position and the camera position can remain unchanged, so the parameters detecting precision can be guaranteed effectively. [ABSTRACT FROM AUTHOR]