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To find the effects of Polygonum cuspidatum on water quality, and the carbon source utilization characteristic of microbial community, this study constructed the ecological floating bed of Polygonum cuspidatum (5% for planting area ratio) in the pond of genetically improved tilapia outside, and assessed the water quality from June to September, 2017. The structure and function of microbial community in water samples of tilapia ponds was analyzed by Biolog technology. The results showed that P. cuspidatum from June to July (during growing period) could effectively reduce the content of TN, TP, Chl.a in water samples of tilapia ponds (P<0.05), but the removal rate of TP in the water sample is not as good as those from August to September (higher temperature period, P<0.05). P. cuspidatum significantly increased AWCD values, McIntosh and Richness (except for September) indexes from July to September (P<0.05) in pond water samples, and which also improved the utilization rate of carbohydrates, amino acids, alcohols by water microbes. P. cuspidatum enhanced carbon source utilization abilities, increased diversity indexes of microbial community in water samples of aquaculture tilapia ponds, which was based on the improvement for water quality of the samples. [ABSTRACT FROM AUTHOR]

Objectives: To explore the relationship between the incidence of rosacea and drinking, smoking, gender or age, and to provide some basis for the diagnosis, treatment and mechanism of rosacea., Methods: A total of 1 180 patients with rosacea and 1 008 non-rosacea patients diagnosed in the Department of Dermatology of Xiangya Hospital were included in the study. Logistic analysis was performed on the incidence factors, and the differences between the two groups in different age groups were compared., Results: There was no significant difference in gender between the two groups ( P >0.05). Logistic analysis showed that drinking had no effect on the incidence of rosacea ( P >0.05); while smoking, gender, and age had an effect on the incidence of rosacea ( P <0.05). The highest proportion of patients with rosacea was 25-34 years old., Conclusions: The incidence of rosacea has nothing to do with alcohol consumption; while smoking, gender, and age affect the incidence. Smoking and women are the risk factors, and the most common age of rosacea is at 25-34 years old.

Objective: To investigate the expression profile and the clinical significance of CDK3 in the tissues of nasopharyngeal carcinoma., Method: Immunohistochemistry was utilized to detect the expression of CDK3 in 94 cases of NPC and 40 cases of nasopharyngeal inflammation., Result: CDK3 was highly expressed in NPC cell lines. Immunohistochemistry showed that CDK3 was mostly expressed in the cytoplasm. The positive expression rate of NPC was 67% and that of nasopharyngeal inflammation was only 12. 5%. The difference between these two groups was highly statistically significant. The CDK3 expression in NPC was related to the TNM clinical staging of NPC (P < 0.01)., Conclusion: The expression levels of CDK3 were obviously higher in NPC tissues. The CDK3 expression in NPC was related to the TNM clinical staging of NPC. It suggests that CDK3 expression may play an important role in the occurrence and development of nasopharyngeal carcinoma.

Soil archaea and fungi play important roles in the greenhouse soil ecosystem. To develop and apply rich microbial resources in greenhouse ecological environment, and to understand the interaction between microbes and plants, we constructed archaeal 16S rRNA and fungal 18S rRNA gene libraries to analyze the compositions of archaeal and fungal communities. Total greenhouse soil DNA was directly extracted and purified by skiving-thawing-lysozyme-proteinase K-SDS hot treatment and treatment of cetyltriethylammnonium bromide (CTAB). After PCR amplification, retrieving, ligating, transforming, screening of white clones, archaeal 16S rRNA and fungal 18S rRNA gene libraries were constructed. The sequences of archaea and fungi were defined into operational taxonomic units (OTUs) when 97% similarity threshold for OTU assignment was performed by using the software DOTUR. Phylogenetic analysis showed that crenarchaeota and unidentified-archaea were the two major sub-groups and only a few of euryarchaeota existed in the archaeal clone library, total 45 OTUs. All the crenarchaeota belonged to thermoprotei; except for Basidiomycotina, the other four sub-group fungi were discovered in the fungal library, total 24 OTUs. The diversities of archaea were very abundant and a few euryarchaeota (methanebacteria) existed in the archaeal clone library, it might be directly related to the long-term high temperature, high humidity, and high content of organic matter. The limitation of oxygen was the other reason for causing this phenomenon; Ascomycotina (over 80%) was the dominant sub-groups in fungal library. It was because most of the plant fungal diseases belonged to soil-borne diseases which gone through the winter by the ways of scierotium or perithecium and became the sources of primary infection.

Objective: In order to search novel nematicidal protease genes, a metagenomic fosmid library was constructed and screened by uncultured method., Methods: Density gradient centrifugation was used to extract and purify total greenhouse soil microbial DNA. After end-repair, ligation, packing and transformation, metagenomic fosmid library was constructed. At the same time, in order to screen the library, function-driven screening was used as a potential strategy, skim milk was served as substrate and root-knot nematodes as targets., Results: The library contained 31,008 clones with the average insert fragment of 36.5 kb, including 1.13 Gbp microbial genetic information, so it was suitable for large-scale microbial functional gene screening. By the function-driven screening, fosmid clone pro12 which contained the nematicidal protease gene was screened. Then, subclones were constructed and screened. A subclone named espro124a5 was screened. After analysis of gene structure, espro124a5 is a secreted extracellular protease and a database search for homologies revealed it possessed 45% identities with peptidase S15 from Maricaulis maris MCS10 (accession no. YP&#95; 756822 at NCBI). It is a novel serine protease. Besides these, it has the serine protease-conserved catalytic triad residues, Asp469, His541 and the catalytic nucleophile Ser348., Conclusion: DNA obtained from the method of Nycodenz density gradient centrifugation had high purity, long fragment, and can meet the requirements of constructing metagenomic fosmid library. At the same time, the metagenomic fosmid library contains a lot of microbial genetic information, which is suitable for the screening of the other microbial genetic resources.