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This article summarized the Traditional Chinese Medicine nursing practice of a case of type 2 diabetes with hypertension during hospitalization. On the basis of western medicine treatment, the intervention was carried out in combination with Traditional Chinese Medicine nursing techniques such as auricular acupoint pressing pills, acupoint application, and Traditional Chinese medicine cream and rubbing, which fully played the characteristics and advantages of Traditional Chinese Medicine technology, effectively controlled the patient's blood pressure and blood glucose, and improve the patient's quality of life. (本文总结1例2型糖尿病合并高血压患者的中医特色护理经验。基于护理评估和中医辨证, 在病情监测、饮食指导、药物管理等常规干预基础上, 配合耳穴压丸、穴位贴敷、中药膏摩等中医护理技术进行干预, 有效控制患者血压、血糖, 提高患者生活质量。)

Objective: To study the impact of Tremella polysaccharide on the ameliorative effect of probiotic-fermented camel milk on immunity and intestinal mucosal barrier function in cyclophosphamide (CTX)-induced immunosuppressed mice. Methods: The physicochemical indexes and antioxidant activities of probiotic-fermented camel milk with and without added Tremella polysaccharide were determined. BALB/C mice were randomly divided into four groups: normal (NC), model (MD), probiotic-fermented camel milk (FCM), and probiotic-fermented camel milk with Tremella polysaccharide (FYCM), which were gavaged with 0.2 mL of normal saline, normal saline, FCM, and FYCM for 30 days, respectively. During the last four days, all groups except the NC group were intraperitoneally injected with a daily dose of 60 mg/kg·mb CTX to establish an immunosuppression model. Organ indexes, the proliferation activity of spleen lymphocytes, delayed-type allergic reaction, cytokine and immunoglobulin contents, spleen and jejunum morphology were analyzed. Results: During 21 days of low temperature storage, the pH of FYCM decreased slowly from 4.52 to 4.30, the titratable acidity increased gradually from 95.7 to 124.73 °T, the number of lactic acid bacteria decreased from 9.40 × 109 to 8.38 × 109 CFU/mL, and the water holding capacity decreased from 39.23% to 35.13%. Compared with FCM, the antioxidant capacity of FYCM was significantly improved (P < 0.01). Furthermore, compared with the MD group, FYCM significantly improved organ indexes, spleen lymphocyte proliferation activity and delayed-type hypersensitivity (DTH), upregulated the levels of interleukin (IL)-6, interferon-γ (IFN-γ), immunoglobulin G (IgG), immunoglobulin M (IgM), and secretory immunoglobulin A (sIgA), and repaired spleen and jejunum mucosal structures, and its effect was significantly more pronounced than that of FCM. Conclusion: Probiotic-fermented camel milk incorporated with Tremella polysaccharide has the potential to regulate immune and intestinal mucosal barrier function, thereby having broad application prospects in the functional dairy market.

U-Net and its variants have showcased exceptional performance in the domain of breast medical image segmentation. By employing a fully convolutional network (FCN) structure for semantic segmentation, the symmetrical structure of U-Net offers remarkable flexibility and adaptability. It can be tailored to diverse image segmentation tasks and challenges by adjusting network depth and incorporating new modules, leaving a significant impact on subsequent network designs. This paper aims to delve into the application of U-shaped convolutional networks in breast medical image segmentation, categorizing and summarizing U-shaped convolutional networks used for this purpose in recent years. It outlines the widely used breast medical image datasets and evaluation metrics, discusses common data augmentation techniques, and provides a detailed introduction to the network structure of the U-Net model along with traditional segmentation methods for breast medical images. Furthermore, it summarizes the improvements made to the U-Net network structure for breast medical image segmentation, including modifications like residual structures, multi-scale features, dilation mechanisms, attention mechanisms, skip connection mechanisms, and integration with Transformers. Finally, it addresses the current challenges and problems encountered in breast medical image segmentation and offers insights into future research directions.

[Objective] The soil erosion issue is very prominent in the karst region of southwest China and significantly affects the sustainable development of the regional economy. However, it is currently not very clear how the characteristic environmental factors of karst basins affect the direct driving force of soil erosion and runoff erosivity. [Methods] Taking the Yeji River Basin in the karst region of southwest China as an example and basing on the long-term and high-frequency field measurement data at the outlet hydrological station of the basin, the spatiotemporal change characteristics of runoff erosivity from 2005 to 2020 were evaluated and the influence of main karst environmental factors on runoff erosivity was analyzed by the Soil and Water Assessment Tool (SWAT) and the Partial Least Squares Model (PLSR). [Results] The average annual runoff erosivity was 2 326.47 m4/(km2·s), with strong spatial heterogeneity. In 2005, the high runoff erosivity was mainly distributed in the northern and central parts of the basin. During 2010—2020, the high runoff erosivity area gradually shifted to the central and southern parts of the basin. In terms of time, benefiting from the implementation of ecological restoration projects such as comprehensive control of soil and water loss in sloping farmland and comprehensive control of rocky desertification since the 11th Five-Year Plan, the runoff erosivity of the Yeji River Basin had been generally decreasing year by year. Lithology, exposed bedrock, and slope were the dominant control factors, which jointly explained 57.7% of the spatiotemporal variation of runoff erosivity in the Yeji River Basin. [Conclusion] These results can provide a theoretical reference for the comprehensive control of soil erosion in karst basins and help to formulate more precise and effective policies and measures to improve the ecological environment of the karst area and promote the sustainable development of the region.

BackgroundIn patients with peptic ulcer bleeding (PUB) , intragastric blood and unavoidable medicine, including acid suppressive agent are suspected as limitary factors to diagnose Helicobacter pylori (H.pylori) infection correctly. The research conclusions about the accuracy of urease breath test (UBT) and stool antigen test (SAT) in patients with PUB are inconsistent.ObjectiveTo clarify the accuracy of UBT and SAT for H.pylori infection in PUB patients.MethodsPubMed, EMBase, the Cochrane Library, Web of Science, CNKI, Wanfang Data Knowledge Service Platform, China Biomedical Literature Database (CBM) were retrieved by computer for relevant articles related to the diagnosis of H.pylori infection by UBT and/or SAT published from the establishment of the database to March 31, 2021. The QUADAS-2 tool was used to evaluate the quality of the extracted literature. The bivariate mixed-effects regression model and network meta-analysis model (NMA) were used to synthesize diagnostic test data. Meta-regression and subgroup analysis were performed to explore the sources of heterogeneity.ResultsA total of 18 articles were included, with a total of 25 studies and 1 105 patients. Meta-analysis results showed that the combined sensitivity of UBT and SAT for diagnosing H.pylori infection in PUB patients were 0.90〔95%CI (0.79, 0.95) 〕 and 0.89〔95%CI (0.81, 0.94) 〕, the combined specificity were 0.91〔95%CI (0.86, 0.95) 〕 and 0.75〔95%CI (0.59, 0.87) 〕, the combined diagnostic odds ratio were 88.89〔95%CI (31.01, 254.82) 〕 and 24.35〔95%CI (13.76, 43.09) 〕, the combined positive likelihood ratio were 10.07〔95%CI (6.07, 16.71) 〕 and 3.60〔95%CI (2.11, 6.12) 〕, the combined negative likelihood ratio were 0.11〔95%CI (0.05, 0.24) 〕 and 0.15〔95%CI (0.09, 0.24) 〕, the area under the SROC curve were 0.93〔95%CI (0.90, 0.95) 〕 and 0.91〔95%CI (0.88, 0.93) 〕. Meta regression showed that the sampling time had an impact on the sensitivity heterogeneity of UBT and SAT, and the sampling time and the H.pylori infection criterion had an impact on the combined specificity heterogeneity of UBT. The Deek funnel chart indicated that there was no potential publication bias among the included researches (PUBT=0.53, PSAT=0.64) .ConclusionIn patients with PUB, UBT had a promising performance for the diagnosis of H.pylori infection. Because of the high number of false-positive results, SAT was not recommended for use in patients with PUB alone. At the same time, in order to avoid the impact of PPI on the detection results, it was recommended to perform the diagnostic tests as soon as possible under the condition of stable hemodynamics.

The current heritage conservation system, fragmented and controlled by administrative units, overlooks the holistic and territorial aspects of heritage resources in China' s Yellow River Basin. Using Moran's I and kernel density estimation, this study unveils the existing spatial distribution characteristics of heritage resources in the Yellow River Basin up to June 2022. It analyzes the spatial distribution differences in diverse geomorphological, climatic, and aquatic environments through geodetic probes. The findings indicate that: (1) The heritage resources in the Yellow River Basin exhibit notable clustering at various scales. A hierarchical patchy distribution appears at the interprovincial scale, high-value clustering at the intermunicipal scale, and an individual scale distribution pattern, described as three central cores radiating into a belt and four subcores dispersing horizontally. (2) The heritage resources in the Yellow River Basin are concentrated in river valley basins and alluvial plains. The suitable natural environment for their distribution includes warm- temperate climates and a low-altitude (0-849 m) continuous surface (the negative zone) formed by tributaries above the first level of the Yellow River. (3) Within the natural environment, geomorphological elements primarily influence the spatial differentiation of heritage resources in the Yellow River Basin. The positive coupling effect of the slope, river network density, and other factors is noteworthy. The dominant differentiation driving factors include absolute elevation (0.33), geomorphological surface elevation (0.30), and temperature zone (0.18). This study' s results are crucial for comprehending the cultural significance of the Yellow River and fostering the sustainable development of heritage resources. [ABSTRACT FROM AUTHOR]

Through the establishment of D-galactose-induced senile osteoporosis (SOP) mouse model, the effect of Grifola frondosa polypeptide-calcium chelate (GPs-Ca) on SOP was explored. The calcium absorption promoting ability of Grifola frondosa polypeptide-calcium chelate was studied by Caco-2 single cell layer model in vitro. Fifty male ICR mice were randomly divided into 5 groups. Except for the blank control group, the other four groups were given intraperitoneal injection of D-galactose: the control group was given intraperitoneal injection of the same dose of normal saline. After 8 weeks, the model was successfully established. Both the control group and the model control group were given deionized water intragastrically: the treated group was given a high dose of Grifola frondosa polypeptide-calcium chelate (Hgps-Ca). After 4 weeks of continuous treated in each group, blood was collected to detect the blood biochemical indicators, and the femur and tibia were stripped to determine the bone indicators. A Caco-2 monolayer was established, and Fluo-3-Am calcium ion probe was used to measure intracellular calcium uptake. Serum calcium and phosphorus levels in the Hgps-Ca group increased significantly, with statistical significance (P<0.05): serum alkaline phosphate (ALP) values of each group were lower than those in the model group, and the serum ALP level of Hgps-Ca group was close to that of the control group. GPs-Ca was more easily absorbed and transported in cells than inorganic calcium. The results showed that Grifola frondosa polypeptide-calcium chelate can improve serum calcium, phosphorus, CAT levels, reduce ALP activity, promote calcium salt deposition, improve calcium absorption and utilization rate, which is conducive to the treatment of SOP. [ABSTRACT FROM AUTHOR]

The emergency department, as an important gateway of epidemic prevention and control in the hospital, bears the responsibility of prevention and control of COVID-19. Hospital highlighted the role of the emergency department in prevention and control of COVID-19. Based on relevant rules and regulations and guidelines of COVID-19 prevention and control, a continuous strategy of prevention and control was implemented according to the actual situation and characteristics of the emergency department. Great efforts were made to enhance the COVID-19 prevention and control, and cluster management measures were carried out including reforming the diagnosis and treatment environment, formulating principals and process of prevention, pre-screening, personnel training, cleaning and disinfection, and supervision of prevention and control. (急诊科作为医院防控疫情的重要关口, 担负着重要的防控职责。本院急诊科高度重视新型冠状病毒肺炎疫情防控工作, 依据国家相关制度及防治指南, 结合医院疫情防控工作实际和急诊科特点, 始终落实预防为主的方针, 加强防控管理, 制订了急诊科集束化管理措施, 包括改造诊疗环境、防控制度和流程的制定、预检筛查、人员培训、清洁消毒、防控督导等。)

Objective To evaluate the significance of risk factors of colorectal polyps recurrence after polypectomy. Method The risk factors of colorectal polyps recurrence after polypectomy in 251 patients were retrospectively analyzed. Results Male, multiple location of colonic polyps （the whole colon） and number of polyps （> 10）were the risk factors for the recurrence of colonic polyps （P = 0.009, < 0.001 and 0.028）. Carcinoembryonic antigen （CEA） level was higher in the recurrence group than the non-recurrence group（P = 0.014）. Age, homocysteine level and number of patients with hypertension in the recurrence group（n = 160） were also higher compared with those in the non-recurrence group（n = 91）, while no statistical significance was observed （P = 0.076, 0.077 and 0.077）. The risk of hyperplastic polyps recurrence was significantly lower （P = 0.023）. No other metabolic factors, such as obesity, diabetes mellitus, fatty liver, triglyceride, LDL, uric acid and colorectal polyps were found to be associated with the recurrence of colonic polyps （P = 0.444, 0.641, 0.541, 0.889, 0.362, 0.247 and 0.248）. Multivariate logistic regression analysis further showed that male and polyps located in the ascending colon were intimately correlated with colorectal polyps recurrence （P = 0.033 and 0.003）.Conclusions Male, multiple location of colonic polyp （the whole colon） and number of polyps （> 10） are risk factors of colorectal polyps recurrence. The risk of hyperplastic polyps recurrence is relatively low. CEA level is positively associated with colorectal polyps recurrence. Male and multiple location of colonic polyp （the whole colon） are significantly associated with colorectal polyps recurrence.

A Ni-Cd alloy plating is applied to the surface of steel component by brush plating, which can realize the double protection of sacrificial anode by cathodic protection and mechanical protection. The effect of concentration of Cd2+ in plating solution on the corrosion resistance of the plating was studied by using the control variable method. The corrosion potential, polarization resistance and corrosion resistance of the coating with different concentrations of Cd2+ were measured by polarization curve, AC impedance spectroscopy, full immersion corrosion test and salt solution immersion test. The results show that the co-deposition of Ni-Cd alloy with Cd of mass fraction of 15%-90% can be realized when the mole fraction of Cd2+ changes from 0.5%-5%, which belongs to abnormal co-deposition. When the molar fraction of Cd2+ is 4 %, that is, the mass fraction of Cd2+ in the coating is 69.40 %, and the corrosion resistance of the alloy brush coating is the best.

In order to rapidly find out causes of failure of underground water pump, a fault diagnosis model of underground water pump based on fuzzy Petri net and condition monitoring was established. Firstly, vibration signal of the water pump was measured by the condition monitoring system of underground drainage equipment, training was carried out on the water pump fault samples after vibration analysis. Then, on the structure of fuzzy Petri net model of water pump fault diagnosis, BP algorithm of neural network was introduced to train parameters such as weight values, threshold values and credibility. The results of instances analysis show that the model can be used to find out the causes of pump failure accurately, and has good accuracy, rapidity and adaptability.

Objectives: To evaluate the application value of CT-based radiomics features for the ascending and descending types of nasopharyngeal carcinoma (NPC)., Methods: A total of 217 NPC patients (48 ascending type and 169 descending type), who obtained CT images before radiotherapy in Affiliated Cancer Hospital of Xiangya School of Medicine, Central South University from February 2015 to October 2017, were analyzed retrospectively. All patients were randomly divided into a training set ( n =153) and a test set ( n =64). Gross tumor volume in the nasopharynx (GTVnx) was selected as regions of interest (ROI) and was analyzed by radiomics. A total of 1 300 radiomics features were extracted via IBEX. The least absolute shrinkage and selection operator (LASSO) logistic regression was performed to choose the significant features. Support vector machine (SVM) and random forest (RF) classifiers were built and verified., Results: Six features were selected by the LASSO from 1 300 radiomics features. Compared with SVM classifier, RF classifier showed better classification performance. The area under curve (AUC) of the receiver operating characteristic (ROC) curve, accuracy, sensitivity, and specificity were 0.989, 0.941, 1.000, and 0.924, respectively for the training set; 0.994, 0.937, 1.000, and 0.924, respectively for the validation set., Conclusions: CT-based radiomics features possess great potential in differentiating ascending and descending NPC. It provides a certain basis for accurate medical treatment of NPC, and may affect the treatment strategy of NPC in the future.

Objective: To evaluate the prognostic value of ultrasonographic measurement of optic nerve sheath diameter (ONSD) and ONSD/eyeball transverse diameter (ETD) ratio in stroke patients during hospitalization., Methods: Adult patients with stroke (ischemic stroke or hemorrhagic stroke) admitted to department of critical care medicine of Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine from November 2017 to November 2018 were enrolled. On the day of admission, ONSD and ETD (retina-retina at 3 mm behind the globe along) were detected by ultrasound, the ONSD/ETD ratio was calculated, and the baseline data and outcomes were recorded. Patients were divided into survival group and death group according to their survival status. Locally weighted scatterplot smoothing (LOWESS) and the receiver operating characteristic (ROC) curve were used to calculate the thresholds of ONSD and ONSD/ETD. The correlation between ONSD, ONSD/ETD and prognosis were assessed., Results: Thirty-eight of 83 patients (45.8%) survived and were discharged from the hospital, while 45 patients died (54.2%). There were significant differences in Glasgow coma score (GCS), shifting of the middle structure, ONSD and ONSD/ETD between the death group and the survival group [GCS: 4.7±2.8 vs. 11.0±3.2, shifting of the middle structure (mm): 5.8±5.9 vs. 1.3±2.6, ONSD (mm): 5.5±0.4 vs. 4.4±0.5, ONSD/ETD: 0.25±0.02 vs. 0.20±0.02, all P < 0.05]. LOWESS and ROC curve analysis suggested thresholds of ONSD and ONSD/ETD for predicting adverse prognosis of stroke patients were 5.0 mm and 0.25, respectively. By adjusting the influence of confounding factors on prognosis, a prediction model based on ONSD was established, and the ROC curve was drawn. The area under the curve (AUC) was 0.978, the optimal predictive point of the model was 0.870, the sensitivity was 89%, and the specificity was 100%. The ONSD/ETD prediction model was also obtained, and the AUC was 0.988, the optimal prediction threshold of the model was 0.768, and the sensitivity for predicting adverse clinical prognosis was 94%, and the specificity was 97%. The stability of ONSD/ETD was better than that of ONSD. ONSD coefficient of variation was 0.14, and ONSD/ETD coefficient of variation was 0.13., Conclusions: ONSD and ONSD/ETD were significantly correlated with the prognosis of critical patients with stroke. The mortality increased significantly in patients with an ONSD greater than 5.0 mm and ONSD/ETD greater than 0.25. ONSD and ONSD/ETD may be promising tools for early assessment of clinical outcomes in these patients.

Objective: To evaluate the safety of cardiac catheterization intervention therapy and transthoracic small incision surgery in the occlusion bydomestic occluder under echocardiography guiding in patients with atrial septal defect (ASD).
 Methods: A total of 1 080 patients with ASD in the occlusion by domestic occluder were analyzed retrospectively, and the interventional treatment were performed in 734 cases through cardiac catheterization intervention therapy and 346 cases through transthoracic small incision surgery. The patients undergone cardiac catheterization intervention therapy were guided under the digital substraction angiography (DSA) and were monitored by transthoracic echocardiography (TTE) in the whole interventional process, and the efficacy was evaluated with TTE. The occlusion of transthoracic small incision surgery was guided under the transesophageal echocardiography (TEE), which was used to monitor the position of occluder and evaluate the efficacy immediately.
 Results: Two kinds of intervention in the occlusion by domestic occluder had achieved satisfactory results in patients with ASD. There was no statistically difference in the longest size of ASD between the 2 intervention methods, while there were statistically differences in the ratio between ASD longest diameter and atrial septal length, and the size of the occlusion, and the disparity between the size of the occluder and ASD longest diameter (D value), respectively (all P<0.05). When the size of arithmetic mean of the ASD was <30 mm, the success rate of the 2 methods was both 100%. When the size of arithmetic mean of the ASD was ≥30 mm, the success rate was 100% in the transthoracic small incision surgery and 50% in the cardiac catheterization intervention therapy.
 Conclusion: Domestic occluder is safe. Compared with the imported one, its cost is lower. When the size of the defects is same, the occlusion is smaller in the transthoracic small incision surgery compared with that in the cardiac catheterization intervention therapy. When the size of arithmetic mean of the ASD is ≥30 mm, the success rate of the transthoracic small incision surgery is higher compared with the cardiac catheterization intervention therapy. When the cardiac catheterization intervention therapy fails, the transthoracic small incision surgery may be a better choice.

Objective: To explore the impact of glycogen synthase kinase-3β (GSK-3β) on Wnt and NF-κB pathways in a rat model of diabetic nephropathy (DN)., Methods: SD rats were randomly divided into normal control group (NC), DN model group (DM) and GSK-3β inhibitor group (DI). Blood glucose and 24-hour urine protein were monitored in three groups. Renal tissue samples were stained by HE. The expression of GSK-3β and NF-κB proteins was studied by immunohistochemistry. GSK-3β and NF-κB mRNAs were detected by RT-qPCR., Results: Ten weeks after STZ injection, the level of blood glucose increased significantly in DM group [(23.2±5.4) mmol/L] and DI group [(25.0±4.0) mmol/L], compared with NC group, and the level of 24-hour urinary protein increased significantly in DM group [(185.2±35.6) g/24 h] and DI group [(179.6±44.7) g/24 h], compared with NC group. Two weeks after LiCl injection, the level of blood glucose and 24-hour urinary protein decreased in DI group (17.6±2.1) mmol/L, (106.9±30.0) g/24 h], compared with DM Group. Compared with NC group, pathological changes of the kidney of DM group aggravated along with increased mRNA and protein expression of GSK-3β and NF-κB. But the pathological changes of the kidney in DI group alleviated along with declined mRNA and protein expression of GSK-3β and NF-κB as compared with DM group (all P<0.05)., Conclusions: NF-κB protein expression positively correlates with the GSK3β expression. Wnt and NF-κB signal pathways play an important role in the development of diabetic nephropathy.

Objective: To investigate the role of Wnt signaling pathway on paraquat (PQ)induced PC12 cells damage., Methods: Using PC12 cells, in this study CCK8 assay was used to detect the effect of cell viability. The cell apoptosis and cell cycle was detected by flow cytometry. The real-time polymerase chain reaction (RT-PCR) was used to measure the mRNA expression of Wnt pathway key genes including Fzd1, Dvl2 and β-catenin and downstream genes including Bax, Bcl2, Survivin, Cyclin D1 and C-myc., Result: Compared with the control, PC12 cells viability in 50.00 and 100.00 µmol/L PQ treatment groups were obviously decreased, the cell cycle S phase arrest, and cell apoptosis increased (P<0.05). The 25.00, 50.00 and 100.00 µmol/L PQ treatment groups mRNA expression of Wnt pathway key genes including Fzd1, Dvl2 and β-catenin and downstream genes including apoptosis suppressor genes (Bcl-2 and survivin)and cyclin gene (Cyclin D1) were downregulated (P<0.05). The mRNA expression of pro-apoptosis gene (Bax) and cyclin gene (C-myc) were upregulated (P<0.05)., Conclusion: It suggested that PQ can activate Wnt pathway to regulate downsteam genes expression, resulting in PC12 cell cycle arrest and apoptosis.

Objective: To investigate the expression of sonic hedgehog (Shh) signaling pathway in liver fluorosis and to explore related mechanism., Methods: To establish animal model, 48 normal SD rats (aged 4-5 weeks) were randomly divided into 4 groups (12 each): control group, fluoriosis group, blocking group and blocking control group. After 6 months, the blocking group and blocking control group were injected intraperitoneally once every 2 days for 3 times with 10 mg/kg cyclopamine or dimethysulfoxide, respectively. Rats were sacrificed at the end of the experiment and the fluoride content in urine and liver function was determined. The expression of Shh and Gli1 protein and mRNA in hepatocytes was detected by immunohistochemistry and real-time fluorescence quantitative PCR, respectively., Results: The fluoride contents in the urine and the incidence of dental fluorosis increased in the fluoride and blocking control groups as compared with those in the control group, but decreased in the blocking group compared with those of the fluoride and blocking control group. Compared with the control group, the titers of aspartate transaminase (AST) and alanine transaminase (ALT) significantly increased, while the activity of total protein and albumin decreased in the fluoride and blocking control groups. Compared with the fluoride and blocking control groups, the activity of the ALT slightly declined and the AST, total protein and albumin slightly increased in the blocking group. Histologically, the cells were disorganized and swollen with cytoplasmic clearing (balloon cells), compared with the control group. The expression of Shh and Gli1 significantly increased in all but the control group. Compared with the fluoride and blocking control groups, the expression of Shh and Gli1 declined in the blocking group., Conclusions: The overexpression and cyclopamine inhibition of the Shh signaling pathway are closely related to the content of fluoride in the liver. The Shh signaling pathway plays an important role in the pathogenesis of liver injury caused by fluorosis, suggesting a preventive and therapeutic target of the disease.

Objective: To investigate the association between different iodine nutrition and the prevalence of dyslipidemia in a representative sample of subjects in Shenzhen., Method: A total of 326 adults from 2 different communities in Shenzhen were included in the study. A questionnaire, physical examination and laboratory test were given to all subjects. 317 questionnaires and blood samples were effective., Results: When the urine iodine concentration was less than 200 microg/L, there is a reverse relationship between iodine nutrition and prevalence of HDL-c (r(s) = - 0.164, P = 0.031). When the urine iodine concentration was higher than 300 microg/L, there is a positive relationship between iodine nutrition and prevalence of HDL-c (r(s) = 0.246, P = 0.013). There was no significant correlation between other lipid parameters and urinary iodine concentration. Multivariate Logistic regression analysis showed that high blood glucose and abdominal obesity were significantly related to the prevalence of dyslipidemia, urine iodine concentration was no significant correlation with the prevalence of dyslipidemia., Conclusion: The prevalence of dyslipidemia in Shenzhen is high, there may be a U shaped relationship between iodine nutrition and the prevalence of abnormal HDL-c level.

Objective: To investigate the effects of spironolactone on the concentration of collagen type I, III in the myocardium of spontaneous hypertension rats (SHR)., Methods: Twenty 8-week male SHR were assigned randomly into spironolactone (SHR-SPIRO, n=10) and control groups (SHR-CON, n=10), sex-age matched Wistar Kyoto rats (WKY group, n=7) were also served as controls. The rats of SHR-SPIRO group were given 20 mg/(kg*d) of spironolactone, the rats of SHR-CON and WKY groups were given the same volume of distilled water. After 16 weeks, the concentration of collagen type I was analyzed with Western blot. The areas of collagen type I and III were observed under polarized light microscopy and the ratio of type I/III collagen was calculated through accumulation score., Results: Compared with WKY group,the concentration of collagen type I in SHR-CON group was significantly higher (1.87 ±0.2 Compared with 1.21 ±0.7, P<0.05). After 16 weeks of treatment the concentration of collagen type I (1.42 ±0.05 Compared with 1.87 ±0.2, P<0.05) and I/III ratio in SHR-SPIRO group were significantly reduced (15.64 ±1.34 Compared with 20.8 ±3.04, P<0.05) compared with SHR-CON group; but there were no differences in accumulation area scores of collagen type III among three groups (368.3 ±30.2 Compared with 481.6 ±32.4 Compared with 406.2 ±45.3, P>0.05)., Conclusion: The deposition of collagen type I in myocardium may be involved in myocardial fibrosis of SHR, and spironolactone can decrease the concentration of collagen type I, which may be one of the mechanisms for its therapeutic effects.

Compatibility chemistry of acid-alkaline pair medicines in formulas of traditional Chinese medicine (TCM) is an important research field which should merit to pay attention. The ideas and methods in prescription compatibility research on formulas containing alkaline-acid pair medicines were summarized from the aspect of chemical groups of alkaline and acid ingredients; the research results were introduced and analyzed; the research meaning was elaborated; and the expectation of the field was viewed.

Objective: To investigate the effects of alcohol and high-fat diet on the expression of PI-3K-P85alpha in rat adipose tissue, and in order to explore the effects on insulin sensitivity and the molecular mechanisms induced by combination of alcohol and high-fat diet., Methods: The rats were treated by alcohol and high-fat. Then the contents of fasting plasma glucose and insulin, and HOMA-IR index of each group were calculated. Expression of PI-3K-P85alpha were detectedby RT-PCR and Western blotting., Results: In comparison with normal diet group, HOMA-IR index of high-fat groups increased, in comparison with high-fat control group, the contents of fasting plasma glucose increased in alcohol combined with high-fat groups, and HOMA-IR increased both in the low-dose and middle-dose alcohol combined with high-fat model groups. Expressions of PI-3K-P85alpha remarkably decreased in all the alcohol and high-fat model groups (P < 0.05)., Conclusion: High-fat diet could induce insulin resistance. Compared with high-fat diet alone, alcohol combined with high-fat could aggravate insulin resistance. The changes of PI-3K-P85alpha expression in adipose tissue may be one of the molecular mechanism of insulin resistance induced by alcohol combined high-fat diet.

Synchronous brain activities are regarded as the indicator of the functional integration of the brain. Based on the physiological mechanism of synchronous activity, the detection process and research on quantification methods concerning synchronization are explained. Conventional and modern signal processing approaches, such as time analysis, frequency analysis, Hilbert transform (HT), especially wavelet transform (WT), are applied to EEG (electroencephalograph) synchronization study. Simulation data and real data are utilized to test the methods mentioned above, which prove the usefulness and validity of methods. According to the final results, Hilbert analysis is better for its authenticity.