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Flavin-containing monooxygenase (FMO) is the key enzyme in the biosynthesis pathway of CSOs with sulfur oxidation. In order to explore the molecular regulatory mechanism of FMO in the synthesis of onion CSOs, based on transcriptome database and phylogenetic analysis, one AcFMO gene that may be involved in alliin synthesis was obtained, the AcFMO had a cDNA of 1 374 bp and encoded 457 amino acids, which was evolutionarily closest to the AsFMO of garlic. Real-time fluorescence quantitative polymerase chain reaction (qRT-PCR) indicated that AcFMO was the highest in the flowers and the lowest in the leaf sheaths. The results of subcellular localization showed that the AcFMO gene product was widely distributed throughout the cell A yeast expression vector was constructed, and the AcFMO gene was ecotopically overexpressed in yeast to further study the enzyme function in vitro and could catalyze the synthesis of alliin by S-allyl-l-cysteine. In summary, the cloning and functional identification of AcFMO have important reference value for understanding the biosynthesis of CSOs in onions.

Objective: To investigate the relationship between SLC22A12 gene rs1529909 and rs7929627 polymorphism and hyperuricemia of residents in Yinchuan, Ningxia province., Methods: Through retrospective analysis in healthy subjects from April to November in 2014, 365 patients with hyperuricemia were selected. 1∶ 1 matched casecontrol study was carried out, matched with age( ± 5 years old), gender, race, and selected 365 people with normal serum uric acid levels as the control group. All subjects were investigated by questionnaire, had a physical examination, and collected fastingvenous blood for biochemical markers and gene polymorphism detection. Paired t test was used to compare the mean of two samples, and the influencing factors were analyzed by conditional logistic regression. The interaction of two sites on hyperuricemia was analyzed by MDR software., Results: The body mass index, blood uric acid, creatinine, total cholesterol and triglyceride in the case group were higher than those in the control group, the difference was statistically significant( P < 0. 05). There was a significant differences between male cases and control group in the allele frequency of rs1529909( χ~2= 4. 887, P = 0. 027). There had a significant difference in the frequency of rs7929627 between the Hui case and control group( χ~2= 14. 906, P = 0. 002), also a significant difference between the male case and control group( χ~2= 9. 749, P = 0. 021). Additionally, theresult of interaction analysis showed that the distribution of high risk and low risk genotype had a statistically significant difference( χ~2= 8. 338, P = 0. 004). The risk of hyperuricemia with high genotype was 1. 536 times of those who had low-risk genotypes( OR = 1. 536, 95% CI1. 147-2. 057)., Conclusion: rs1529909 and rs7929627 were associated with hyperuricemia among men. rs7929627 was related to it in the Hui. There may be a strong antagonistic effect on hyperuricemia between rs1529909 and rs7929627.