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In order to meet the demand of high-gain direct-current boost converter in the process of using new energy, a novel quadratic boost direct-current converter is proposed. In this paper, the quadratic boost direct-current converter compared to the traditional quadratic boost direct-current converter and boost circuit, have a higher voltage gain and lower switching tube voltage stress, which is more practical for high energy conversion efficiency , The voltage gain requirements of the new energy and network applications. The correctness of the theoretical analysis is proved by the experimental model of the laboratory.

Objective To explore the active components, targets and possible mechanism of Yishen Tongluo (Kidney-boosting Collateral-unblocking) Formula (YSTLF) in the treatment of diabetic kidney disease (DKD) based on network pharmacology. Methods The effective components and related targets of YXTLF were screened from the Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform ( TCMSP), The Encyclopedia of Traditional Chinese Medicine ( ETCM) and A Bioinformatics Analysis Tool for Molecular mechanism of Tradition Chinese Medicine (BATMAN-TCM), and further supplemented with literature research. Then these components and targets were associated with the target information related to DKD in GeneCards, OMIM, TTD and DrugBank databases. Based on the protein-protein interaction (PPI) network constructed by String11. 0 software, the key targets corresponding to the main active components in YSTLF were obtained. The "component-disease-target" regulatory network diagram with TCM compounds was constructed by cytoscape 3. 8. 0, and the pharmacodynamic mechanism of YSTLF in the treatment of DKD was preliminarily predicted. At the same time, gene ontology (GO) enrichment analysis and Kyoto Encyclopedia of Gene and Genome (KEGG) enrichment analysis were carried out using R language BioConductor and pathview software to reveal the functions of related target genes and pathways. With the mesangial cells ( SV40-MES-13) of mice induced by high glucose as the main research object, the effects of YSTLF on the expression of key target genes and the regulation of related pathways were verified by luciferase reporter gene detection, RT-qPCR and western blot. Results 108 active components of YSTLF with potential therapeutic effect of DKD and 417 corresponding targets were screened. The " component-disease-target" network diagram suggested that kaempferol and astragaloside were the main active components in YSTLF in the treatment of DKD, and Akt and IL-6 were the key targets of the above-mentioned chemical components. The result of GO functional annotation and KEGG pathway enrichment showed that the selected key targets were mainly involved in a series of biological reactions, such as drug immune response, oxidative stress response, inflammatory response, and signal transduction, etc., and were mainly involved in the regulation of AGE-RAGE and PI3K/ AKT signal pathways. The result showed that YSTLF could effectively improve the activation of inflammation related indexes TNF-α and IL-6 and fibrosis related indexes TGF-β1, α-SMA and Col-I in SV40-MES-13 cells in mice induced by high glucose. Conclusion Yishen Tongluo Formula seems to be multi-component, multi-target and multi-pathway in the treatment of DKD. It may play a role in the treatment of DKD by regulating AGE-RAGE and PI3K/ AKT signal pathways, thus inhibiting inflammatory reaction and renal tissue fibrosis. [ABSTRACT FROM AUTHOR]

Objective: To clone and analyze variable region gene sequences of mouse monoclonal antibody against human S100A9( anti-human S100A9 mAb)., Methods: Total RNA was extracted from the hybridoma cell line( Ⅱ D4B10)secreting mouse anti-human S100A9 mAb. Furthermore,the heavy chain variable region( VH) and light chain variable region( VL) genes were amplified using reverse transcription-polymerase chain reaction( RT-PCR). After the cloning and sequencing of the VHand VLgenes,the sequence analyses were performed by BLAST program., Results: The VLgene,a member of the mouse IGKV14-111* 01 family,had an open reading frame( ORF) of 291 bp with 97 amino acids,and the VHgene,a member of the mouse IGHV1-80* 01 family,had an ORF of 324 bp with 108 amino acids. In addition,the sequences of VH and VLgenes presented the characteristics of antibody variable region with individual frameworks and complementarity determining regions., Conclusion: The variable region gene sequences of mouse anti-human S100A9 mAb have been successfully obtained,which are helpful to construct genetically engineered antibody in the future.