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Magnetic fields can improve the quality of foods by influencing the arrangement of water molecules, thus altering the distribution of water in foods. In this study, dough was fermented at magnetic field strengths of 0, 0.5, 1.0, 1.5, and 2.0 mT for 1 h. The effect of magnetic field treatment on the fermentation characteristics, moisture distribution, and protein molecular structure of dough was investigated by low-field nuclear magnetic resonance (NMR) and in situ Raman spectroscopy, with a view to providing a theoretical basis for the development of high-quality Chinese dough-based foods. The results showed that the fermentability of dough tended to increase first and then decrease as the magnetic field strength increased, and the most significant effect on the fermentability was observed at a magnetic field strength of 1.5 mT. The specific volume of steamed bread initially increased and then decreased. When the magnetic field strength was 1.0 mT, the specific volume was 2.24 mL/g. The hardness, stickiness, and chewiness showed a trend of initially decreasing and then increasing, but there was no obvious change in the elasticity, cohesiveness or resilience. The contents of strongly bound water and free water initially decreased and then increased, whereas the opposite trend was observed for weakly bound water. The effect of 1.0 mT magnetic field strength on the water distribution was most significant. The relative content of α-helix increased first and then declined, while the reverse trend was observed for random coil. At a magnetic field strength of 0.5 mT, the relative content of α-helix was the highest (54%) and the relative content of random coil was lowest (21%). Proper magnetic field treatment could increase the fermentability of dough, the specific volume and textural properties of steamed bread, accelerate the water distribution in dough and improve the stability of the gluten network. In conclusion, magnetic field treatment could improve the fermentability of dough and the textural properties of steamed bread by improving the distribution of water, increasing the moisture uniformity, and changing the molecular structure of gluten proteins in dough.

Differential scanning calorimetry (DSC), low-field nuclear magnetic resonance (NMR) and in situ Raman spectroscopy were used to explore the cryoprotective effect of enzymatic hydrolysates of flaxseed meal proteins obtained at different hydrolysis times on dough. The results showed that the thermal hysteresis activity of the 60 min hydrolysate, with a 13.62% degree of hydrolysis (DH), was highest (0.83 ℃), and the ice recrystallization inhibitory activity was also highest, minimizing the proportion of large ice crystals. It was found that addition of the hydrolysate had the most significant cryoprotective effect on dough. Compared with the control group, the proportion of strongly bound water in dough increased by 13.2%, and the proportions of weakly bound water and free water decreased by 0.7% and 7.9%, respectively. The maximum fermentation height, the final height and the onset time of CO2 leakage increased by 18.5%, 24.4% and 34.4%, respectively. Protein structure analysis showed that the relative α-helix content of gluten proteins in dough with the hydrolysate was highest and increased by 35.3% compared with the control group, and the relative random coil content decreased by 28.9%. The intensity (I760) of tryptophan band at 760 cm-1 increased by 282.3%, and the ratio (I850/I830) between the intensities of tyrosine bands at 850 and 830 cm-1 decreased by 59.6%. The relative contents of disulfide bond configurations g-g-g and g-g-t were increased by 8.8% and 9.8%, respectively. In conclusion, enzymatic hydrolysates of flaxseed meal proteins improved the water-holding capacity of dough, shortened dough proofing time, and weakened the damage of ice crystals to the network structure of dough, and the effect of the hydrolysate with DH of 13.62% was most pronounced.

In order to reveal the protective mechanism of hydrocolloid on wheat dough gluten during freezing, Fourier transform infrared (FTIR) spectroscopy and scanning electron microscopy (SEM) were used to investigate the secondary structure, microstructure, disulfide bond content and water-binding capacity of gluten obtained from frozen-thawed wheat dough added with different amounts of flaxseed gum (FG) or Artemisia sphaerocephala Krasch. gum (ASKG). Results showed that freezing-thawing treatment destroyed the ordered network structure of gluten, while FG and ASKG could cross-link with the gluten protein network structure to form a stable cross-linked structure. Upon the addition of FG or ASKG at 0.4%, the gluten network structure was most uniform and dense. The addition of FG and ASKG increased the content of disulfide bond and the relative content of α-helix in gluten, making the gluten structure more stable under repeated freeze-thaw cycles. In addition, the addition of FG and ASKG reduced the surface hydrophobicity and consequently led to an increase in the water-binding capacity of wheat gluten. The highest water-binding capacity was obtained at an addition level of 0.4% for both FG and ASKG, 1.46 and 1.43 times higher than that of the control group without added hydrocolloid undergoing repeated freeze-thaw cycles, respectively. All these results showed that FG and ASKG could interact with gluten to form a stable cross-linked structure, thereby enhancing the water binding capacity of gluten protein and exerting a cryoprotective effect on it.

Potato protein deaminated for different durations (0, 0.5, 3, 6 and 12 h) were used to prepare emulsions, and the products obtained were evaluated for droplet size distribution, emulsion stability, microrheological properties and microstructure. The results showed that the droplet volume mean diameter and Turbiscan stability index (TSI) of the emulsion decreased first and then increased with increasing deamidation time. The emulsions with potato protein deaminated for 3 and 6 h exhibited uniform droplet size distribution and good stability, which may be because the increased electrostatic repulsion between the modified protein emulsion droplets prevented their aggregation. The droplet size of the emulsion with potato protein deaminated for 12 h increased and the stability decreased, which may be because of the decreased charge repulsion force and consequent droplet aggregation. The microrheological analysis showed that the emulsion with potato protein deaminated for 6 h had the highest macroscopic viscosity index (MVI) value and the highest viscosity, and the speed of droplet movement was slowed down. There was a strong force between the emulsion droplets, which made the system more stable. This study can provide a reference for further research and development of potato protein products in order to improve the utilization rate of potato protein and expand the application range.

In this experiment, confocal laser Raman spectroscopy and low-field nuclear magnetic resonance imaging were used to in situ characterize and analyze the molecular structure changes of gluten proteins and water migration during the freezing process of dough. The results showed that the disulfide bond configuration of gluten proteins in dough with 45% water content was the most stable, and the relative percentage of the gauche-gauche-gauche (g-g-g) configuration decreased by 4.33% after freezing. During the freezing process, the relatively stable g-g-g configuration was transformed to unstable gauche-gauche-trans (g-g-t) and trans-gauche-trans (t-g-t) configuration. The ratio between the intensities of the absorption peaks at 740 and 1 004 cm-1 (I740/1 004) in the Raman spectrum, which represents the microenvironment around the side chains of amino acids, reached the maximum when 45% water was added to dough, and decreased continuously during freezing. At the end of freeze, there was no significant difference in the relative percentage of α-helix in the secondary structure of gluten proteins with water addition, but the relative percentage of α-helix decreased by 3.63% during the freezing process. The results of water distribution and migration showed that the quality deterioration caused by ice crystal growth appeared earlier with the increase of water addition during the freezing process of dough. The results of this study will help to clarify the mechanism of dough quality deterioration during freezing.

Objective To investigate the effect of circular RNA hsa_circ_0001922 on the proliferation, migration and invasion of prostate cancer cell PC-3 and its underlying molecular mechanism. Methods qRT-PCR and RNA FISH were used to detect the expression level and localization of hsa_circ_0001922 in PC-3 cells respectively. After hsa_circ_0001922 was targeted inhibited, clone formation, Transwell assay and scratch assay were used to detect the proliferation, migration and invasion abilities of PC-3 cells. qRT-PCR and Western blot were used to detect the expression levels of EMT pathway-related molecules after inhibiting hsa_circ_0001922. Results The expression of circular RNA hsa_circ_0001922 was increased in PC-3 cells (P < 0.01) and it existed in the cytoplasm and nucleus. The invasion, migration and invasion abilities were significantly weakened (P < 0.05) after inhibiting hsa_circ_0001922; the expression of E-cadherin mRNA increased (P < 0.05) while the mRNA level of Vimentin decreased (P < 0.05). The results of Western blot were consistent with the above (both P < 0.05). Conclusion The circular RNA hsa_circ_0001922 may promote the proliferation, migration and invasion of PC-3 cells through the EMT pathway.

Plant hormone Abscisic Acid (ABA) plays an important role in regulating plant growth. However, the content of ABA in plant tissues is very low, and rapid and sensitive detection methods are urgently needed. In this study, a rapid and quantitative ABA detection method was established based on aptamer recognition and surface-enhanced Raman spectroscop (SERS). The gold nanoparticles modified by ABA aptamer had the characteristics of SERS signal enhancement and selective recognition, realizing the rapid and sensitive detection of trace ABA in complex plant sample matrix. When ABA molecules appeared in detect system, the aptamer would specifically bind with ABA molecules, and the aptamer folded into G-tetrad structure at same time, which wrapped ABA molecules in the tetrad structure, shortened the distance between ABA molecules and gold nanoparticles, and the enhanced and stable ABA molecules SERS signal were obtained. Under the condition of optimized aptamer concentration at 0.12 µmol/L, different concentrations of ABA solutions in the detection system were detected. Within the concentration range of 0.1－100 µmol/L, the SERS intensity of ABA presented a good linear relationship with the concentration. The detection limit of this method was 0.1 µmol/L and the linear correlation coefficient R2 was 0.9855. The repeatability test of 20 points randomly on SERS substrate showed that the relative standard deviation (RSD) was 6.71%, indicating the stability of SERS substrate was well. Furthermore, the substrate of gold nanoparticles modified by the ABA aptamer terminal with sulfhydryl group (SH-Apt) could be stored in the refrigerator for more than half a year, indicating that the substrate has good stability. Once the preparation of the synthesized SH-Apt modified gold nanoparticles was completed. It could be used on demand without the need to prepare SERS substrate for every detection. In this sense, the constructed aptamer SERS biosensor could realize the rapid and quantitative detection of ABA. The method was used for the determination of ABA in wheat leaves, and the result was in good agreement with the Enzyme Linked Immunosorbent Assay (ELISA) (The max relative error was 9.13%). This biosensor is an exploratory study on the detection of plant hormones by SERS, and the results of the study will have important reference value for the subsequent quantitative and on-site detection of ABA, as well as the detection of other plant hormones.

This paper reviewed the nursing experience of an elderly patient with ischemic cerebrovascular disease complicated with pulmonary infection. On the basis of routine treatment and nursing intervention of pulmonary infection, the traditional Chinese medicine aerosol inhalation nursing was given. The implementation of atomized inhalation of traditional Chinese medicine can obviously reduce the viscosity of sputum of patients, and reduce the number of times of daily sputum aspiration. (本文回顾1例缺血性脑卒中并发肺部感染老年患者的护理经验。在常规肺部感染治疗、护理干预的基础上, 给予中药雾化吸入。中药雾化吸入能够有效降低患者痰液的黏稠度, 减少患者每日吸痰次数。)

Objective To systematically sort out the research literature on cerebrovascular angiography nursing in my country, to discuss the research status, hot spots and development trends of cerebrovascular angiography nursing, and to provide references for clinical staff engaged in cerebrovascular angiography nursing in China. Methods Taking the nursing of cerebral angiography as the subject, searching domestic common databases including CNKI, VIP, Wanfang, and using CiteSpace software to visually analyze the retrieved documents. Results A total of 1088 articles were included, combined with keyword co-occurrence and burstiness, etc. , it was concluded that the research in the field of cerebral angiography in my country mainly focuses on the effectiveness of different nursing methods and new nursing techniques in reducing perioperative complications. Conclusion Based on evidence-based, graded assessment of the risk factors that cause urinary retention after cerebrovascular angiography and individualized response measures based on different risk grading standards, continuous optimization of nursing intervention methods, and increased patient comfort are the future Research trends. (目的 系统梳理我国有关脑血管造影术护理的研究文献, 探讨脑血管造影术护理的研究现状、热点及发展趋势, 为我国从事脑血管造影术护理的临床工作人员提供参考。方法 以脑血管造影术护理为主题, 检索国内常用数据库包括中国知网、维普、万方, 运用CiteSpace软件对检索到的文献进行可视化分析。结果 共纳入1088篇文献, 结合关键词共现及突现词等, 得出我国脑血管造影术护理领域的研究主要围绕不同的护理方式以及护理新技术等对减少围手术期并发症有效性方面展开。结论 以循证为基础, 对引起脑血管造影术后尿潴留的危险因素进行分级评估, 针对不同的风险分级标准开展个性化的应对措施, 不断优化护理干预方式, 增加患者舒适性等是未来研究的趋势。)

To study the effect of resting on the quality of frozen dough, the changes in the moisture state of the dough during the resting process (0, 30, 60, 90, 120 min), the moisture distribution of dough and their effects on ice crystal distribution, microstructure, and creep recovery characteristics after dough freezing were investigated. The results showed that the freezable water content in the dough was decreased by 0.96% with the extension of the resting time. The pores and number of ice crystals formed in the dough during the freezing process gradually decreased and the distribution of ice crystals became more and more uniform. The average pore area decreased from 22.65 to 11.80 with the extension of the resting time from 0 min to 120 min, and the number of pores decreased from 22.65 to 11.80 with the extension of the resting time. The maximum creep flexibility Jmax increased significantly (P<0.05) and the zero shear viscosity η0 decreased significantly (P<0.05) during the creep phase as the resting time was extended. After resting for 120 mins, the maximum creep flexibility Jmax of frozen dough decreased by 0.50x10-4 Pa-1zero shear viscosity η0 decreased by 0.43 x 105 Pa-1. This indicated that the hardness of frozen dough decreases and the shape of the dough was easier to maintain. During the recovery process, the instantaneous flexibility J0 and viscoelastic flexibility Jm increased by 1.26x10-4 and 2.88 x 10-4 Pa-1, respectively, and the recovery force of the frozen dough increased. In summary, resting process of dough would reduce the content of freezeable water, improve the water holding capacity, and reduce the damage of ice crystals to the dough network structure in the freezing process to improve the quality of the frozen dough. [ABSTRACT FROM AUTHOR]

The tire loaders produced by a certain unit are found abnormal noise when they are tested in the factory. Through the contrast,noise analysis and calculation of noise with and without abnormal sound loaders at different speeds,the cause of abnormal noise is detected,and the diagnosis opinions and suggestions are put forward so that the problems can be solved.

The changes of freezing curve and moisture uniformity during the freezing process of dough with different water(40%, 45%, 50% and 55%) additions at -18 ~ 25 T were studied, and the effects of ice crystal growth on the network structure of gluten protein and the chemical forces during dough freezing were studied with 45% water addition as an example. The results showed that: the moisture uniformity showed a decreasing trend with different water additions during the dough freezing process. With the increase of water addition, the freezing rate of the dough first increased and then decreased, and the freezing rate was fastest when the water addition was 45%. With the decrease of temperature, the number decreased during the freezing process, the average pore area of ice crystals in the dough with 45% water addition increased, and it increased rapidly in the temperature range of -6 °C to -9 °C; The number of pores decreased continuously, the branching rate of gluten protein network structure parameter gradually decreased from 2.20x10-3 to 1.90x10-3, and the endpoint rate gradually increased from 2.10x10-3 to 2.40x10-3. The hydrogen bonds, ionic bonds, and disulfide bonds between gluten protein network gradually weakened, and the gluten protein network was destroyed. [ABSTRACT FROM AUTHOR]

Flaxseed gum (FG) and Artemisia sphaerocephala Krasch Gum (ASKG) were added into refined wheat flour to prepare frozen dough, and were treated with freeze-thaw cycle. The effects of these two hydrophilic colloid on the silty properties and gelatinization properties of wheat mixed flour, the water distribution and state, freezable water content (FW) and the microstructure of gluten protein in the frozen dough after freeze-thaw cycle treatment were investigated. The influences of these two hydrophilic colloid on specific volume (SP), stomata structure, texture and sensory quality of steamed bread were studied. The results showed that compared with blank control group, hydrophilic colloid could improve water absorption and flour quality index during dough formation, reduce the weakening degree of wheat mixed powder, and the stability time during dough formation was extended after ASKG was added. Hydrophilic colloid could improve the peak viscosity, disintegration value and recovery value of wheat mixed paste system, and the effect of ASKG was more significant than that of FG. The hydrophilic colloid could increase the strongly bound water content (A21) and decrease the free water content (A23) in the frozen dough. Under the same addition level, the ability of FG to regulate water distribution in the frozen dough was stronger than that of ASKG. Hydrophilic colloid could decrease FW in frozen dough and improve its frost resistance. Hydrophilic colloid could increase the SP, elasticity and resilience of steamed bread cooked by frozen dough, and reduce its hardness and chewability, that is, adding hydrophilic colloid could slow down the damage of gluten network structure caused by freeze-thaw cycle treatment, maintain its integrity and stability, improve the internal structure of steamed bread, and give it a soft and elastic texture. [ABSTRACT FROM AUTHOR]

Objective To explore the correlation between pulmonary function and chest radiograph fibrosis score among patients with pneumoconiosis. Methods A total of 100 patients with pneumoconiosis treated at the Shandong Academy of Occupational Health and Occupational Medicine from November 2020 to April 2021 were studied. The chest digital radiography of these patients was evaluated, and the fibrosis score was assessed based on the density of shadow in each lung area on the chest X -ray. The relationship between the score of pulmonary fibrosis and pulmonary function was analyzed. Results Among 100 male cases of pneumoconiosis, 37 cases were in the first stage, 41 cases in the second stage, and 22 cases in the third stage. 34 cases had lung function impairment, including 22 mild, 11 moderate, and 1 severe case, with an incidence of 27.02% in the first stage, 29.26% in the second stage, and 54.54% in the third stage; the difference was significant (P < 0.05). The fibrosis score increased with the pneumoconiosis stage (P < 0.01), and the forced vital capacity (FVC), forced expiratory volume in one second (FEV1), FEV1/FVC, 75% maximal expiratory flow (MEF75), and 50% maximal expiratory flow (MEF50) decreased (P < 0.05). The scores of pulmonary fibrosis were negatively correlated with FVC, FEV1, MEF75, MEF50, 25% vital capacity (MEF25), and the single breath diffusing capacity for carbon monoxide (DLCO SB) (r = - 0.339, - 0.431, - 0.334, - 0.362, - 0.369, - 0.245, P < 0.05). The patients with more severe pulmonary function had a higher score of fibrosis (P < 0.05). Conclusions The fibrosis score on the chest radiograph of pneumoconiosis patients is a useful indicator for the clinician to evaluate the lung function damage caused by pneumoconiosis. [ABSTRACT FROM AUTHOR]

With yeast cell as research objective, the effects of different amounts of gluten antifreeze polypeptides on yeast cell survival rate, cell morphology, cell membrane damage and leakage of cell contents during the freezing or repeated freezing and thawing process were studied. The results showed that a certain mass concentration (20 ~ 40 g/ L) of gluten antifreeze polypeptides could improve the survival rate of yeast cells during the freezing or repeated freezing and thawing process. After repeated freezing and thawing 8 times, the cell survival rate in the yeast suspension supplemented with 40 g/ L gluten antifreeze polypeptide was the highest, which was 65. 63% higher than that in the blank group. Gluten antifreeze polypeptide could slow down the destruction of yeast cells by ice crystals and keep yeast cells relatively intact and smooth surface morphology. The gluten antifreeze polypeptides significantly reduced the proportion of damaged cells during the freezing or repeated freezing and thawing process, and inhibited the leakage of intracellular DNA and GSH to a certain extent. Gluten antifreeze polypeptides improved the survival rate of yeast cells by reducing the adverse effects of ice crystals on cell membranes during the freezing or repeated freezing and thawing process. [ABSTRACT FROM AUTHOR]

In order to investigate the effects of hydrophilic colloid (Artemisia sphaerocephala Krasch. gum (ASKG)) on the gelatinization and rheological properties of waxy rice starch, ASKG was mixed with waxy rice starch at different ratio (0.1%, 0.3%, 0.5%, 0.7% and 0.9%). The results showed that the addition of ASKG could increase the gelatinization viscosity and swelling degree of waxy rice starch, and decrease the gel strength and water fluidity of waxy rice starch. Waxy rice starch had the properties of pseudoplastic fluid. The addition of ASKG could increase the apparent viscosity, consistency coefficient (K) and flow characteristic index (n), indicating that ASKG had synergistic thickening effect. ASKG could improve the elastic modulus (G'), viscosity modulus (G") and loss angle tangent value (tan δ) of waxy rice starch, but the overall performance was to enhance the fluidity of the system. [ABSTRACT FROM AUTHOR]

Mung bean starch was modified by heat moisture treatment (HMT). The effects of HMT on the structural properties (crystalline and short-range ordered molecular structure) and physicochemical properties (thermodynamics, pasting and digestion properties) of samples with different moisture contents (15%, 20%, 25% and 30%) were studied. The results showed that the crystal type of mung bean starch was not changed by HMT, but the crystallinity and short-range ordered molecular structures decreased. With the increase of moisture content, the gelatinization, peak and termination temperature of mung bean starch increased, while the peak viscosity, final viscosity and setback decreased. These results showed that the thermal stability of starch was enhanced after HMT. Compared with the original mung bean starch, the content of resistant starch was significantly increased by HMT. [ABSTRACT FROM AUTHOR]

Based on the knowledge of starch structure and retrogradation mechanism, this study evaluated the impact of exogenous components on starch retrogradation. As well, multiple analytical techniques including thermal analysis, spectral analysis and microscopic imaging were applied to determine the retrogradation of starch from macroscopic and molecular level. This study indicated that starch retrogradation could be affected by various exogenous components such as non-starch carbohydrate, salt, protein and lipid. However, starch-based foods was a complicated system and each component may interact with starch molecules during storage, thus it was necessary to further investigate the mechanism of starch retrogradation under complicated model changes rather than simple model system. Furthermore, current researchers normally revealed the starch retrogradation with single analysis technology, and common analysis techniques still have limitations such as single analysis index and difficulty in operation. Therefore, it was invaluable to measure starch retrogradation behaviors with combined techniques according to the information of food samples in practical application, which was helpful for a comprehensive understanding on the changes of starch retrogradation properties, hence impeding the retrogradation degree of starchy-foods and prolong their shelf-life. [ABSTRACT FROM AUTHOR]

The effects of different freezing storage time (0 d, 10 d, 20 d, 30 d) on the water content (Fw), water distribution and existence state, thermodynamic characteristics and texture characteristics in the dough were studied. And the correlation between them was explored through principal component analysis and correlation analysis. The results showed that the moisture state of the dough changed significantly with the increase of the frozen storage time. Fw, strongly-bound water content (A21) and free water (A23) increased, the lateral relaxation time of weakly-bound water (T22) and free water (T23) in the dough shifted to the left. After frozen storage, the thermal stability (To, Tc and ΔH) of the dough was improved, the hardness, gumminess and chewiness were increased, and the cohesiveness was reduced. The change of the internal moisture and thermal characteristics of the dough during the frozen storage period caused the improvement of the change value of the dough texture properties PC1, which represented the dough quality deteriorated. [ABSTRACT FROM AUTHOR]

Bamboo shoot dietary fiber was added into dough to study the processing characteristics and moisture distribution of the repeated freeze-thaw dough. The results showed that the hardness of the dough in the control group increased first and then decreased, the viscosity and the chewiness increased, the elasticity became smaller. However, the hardness, viscosity and elasticity of the dough added with BSDF had not been significantly affected during the process of repeated freezing and thawing. When the number of repeated freeze-thaw cycles exceeded one time, the modulus of elasticity and viscosity of the BSDF dough increased, and the loss angle tangent decreased. During the process of repeated freezing and thawing,the deep layer bound moisture content of the dough in the control group decreased, while the weakly bound water and free water content increased. However, the addition of BSDF made the deep layer bound moisture content increased, the weakly bound moisture content decreased, and the free moisture content did not change significantly. [ABSTRACT FROM AUTHOR]

Objective To investigate the effects of Danggui Buxue Tang (DGBX, angelica sinensis blood-tonifying decoction) on human endothelial cells in a steady flow shear stress (FSS). Methods Cells were incubated on a parallel plate flow chamber at a steady FSS of 0, 0.12, 1.2, and 2.4 Pa for 6 hours. Cells exposed to different FSS were randomly divided into 5 groups: control group perfused with M199 culture medium, simvastatin group (0.1 µmol/L simvastatin), and DGBX groups {3 g/L DGBX decoction: Chinese angelica (Danggui, Radix Angelica Sinensis, AS) and astragalus root (Huangqi, Radix Astragali, RA) at the dose ratios of 1:1, 1:3, and 1:5}. After 12 hours, the cellular proliferation, levels of nitric oxide (NO), expressions of endothelial nitric oxide synthase (eNOS), vascular endothelial growth factor receptor 2 (VEGFR2) mRNAs and proteins were measured. Results In control group, eNOS and VEGFR2 levels in the endothelial cells exposed to FSS at 1.2 and 2.4 Pa were significantly higher than those of cells exposed to FSS at 0 and 0.12 Pa(P < 0.05). Compared with the control group, DGBX decoction up-regulated the cellular proliferation and expression of eNOS and VEGFR2, and NO levels at FSS of 0 and 0.12 Pa (P <0.05). And DGBX decoction (AS-RA at 1:3, 1:5) up-regulated the expressions of eNOS and VEGFR2, cellular proliferation and NO level at FSS of 1.2, 2.4 Pa (P <0.05). Conclusion FSS seems to play an important role in the effects of DGBX decoction on endothelial cells. [ABSTRACT FROM AUTHOR]

Objective To investigate the quality of life of cleft lip and/or palate children's parents and discuss the factors to provide the oretical basis for improving the quality of life of these parents and promoting the healthy growth of children with cleft lip and/or palate. Methods A total of 115 parents whose children had cleft lip and/or palate surgery treatment were selected as the experiment group, and another 198 parents (with healthy children having a similar age with those in the experiment group) as the control group. The experiment group was divided into three subgroups according to different types of cleft lip and/or palate: cleft Lip (CL), cleft palate (CP), cleft lip and palate (CLP). The experiment group and the control group were both divided into four subgroups according to age:0-1, 1-3, 3-6 years old, and more than 6 years old. The experiment group and the control group were both divided into three subgroups according to education: junior middle school and the following, high school and technical secondary school, junior college degree or above. The GQOLI-74 scale was selected to assess the experiment group and the control group. SPSS 16.0 software was used to analyze data. Results 1) The experiment group had no significant difference with the control group in terms of the overall score and the scores of various children ages. 2) The scores of every item had no significant difference in CL, CP, CLP subgroup (P>0.05). 3) The quality of life scores and scores of psychological function dimension and social function dimension of parents with 3-6 years old patients were obviously lower than those of parents with more than 6 years old patients (P<0.05). The scores of social function dimension of parents with 0-1, 1-3, 3-6 years old patients were obviously lower than those of parents with more than 6 years old patients (P<0.05). The other items had no significant difference. 4) The scores of material life dimension and social function dimension of parents with junior college degree or above were higher than those of parents with junior middle school degree and the following (P<0.05). The scores of social function dimension of parents with high school and technical secondary school degree were higher than those of parents with junior middle school degree and the following (P<0.05). Conclusion No difference was observed in the quality of life between cleft lip and/or palate children's parents and normal group. The parents with the low age children with cleft lip and/or palate and low-levels of education need more help and support to improve quality of life. [ABSTRACT FROM AUTHOR]

The leaves and roots of Sarcandra glabra (thunb) nakai have different therapeutic effects in some clinical applications. In order to explore the tissue specific distribution differences of terpenoids in the leaves and roots of S . glabra , and to analyze the molecular mechanism of the formation of their pharmacodynamic quality differences. In this study, liquid chromatography-mass spectrometry (LC-MS) and Illumina HiSeq TM high-throughput sequencing techniques were respectively used to obtain the metabolome and transcriptome data of the leaves and roots of S . glabra . The metabolomics analysis showed that there were 50 differential terpenoids metabolites between the leaves and roots, including farnesylcysteine, d-glyceraldehyde 3-phosphate, and (R)-5-phosphomevalonate. The transcriptomics analysis indicated that there were 57 differentially expressed metabolic enzyme coding genes, including ACTC , HMGCR , MVK , DXS , and KS . Moreover, there were seven transcription factors, including MYB , C2H2 , AP2/ERF-ERF , which were predicted to participate in regulating the differences in terpenoid synthesis and accumulation between the leaves and roots of S . glabra . qRT-PCR results demonstrated that the expression changes of eight randomly selected enzyme genes involved in terpene synthesis between the leaves and roots of S . glabra , which were consistent with the transcriptome sequencing results. This study will help to elucidate the molecular mechanisms underlying the clinical efficacy differences between the leaves and roots of S . glabra , and facilitate the extraction, utilization, and resource development of S . glabra .

Coronoid process hyperplasia (CPH) is a rare condition characterized by the increase in the size of the coronoid process, mainly occurring in the second decades of human life. To provide a reference for the diagnosis and treatment of this disease, we reported the clinical manifestations of two cases, X-ray examination, treatment, and prognosis with mandibular movement and restricted mouth opening (RMO) caused by bilateral CPH. The opening degree of the two cases described in the article increased to almost 40 mm immediately after the elongated coronoid process was surgically removed and a case was maintained normal after 2 months. In another case, CPH recurred after 2 years of follow-up. The etiology of CPH is still unclear, which is associated with various factors. RMO caused by CPH is extremely rare in clinical practice, and it is easy for patients to get confused and be misdiagnosed. It should be diagnosed using comprehensive methods of clinical studies and imaging modalities. Currently, surgical coronoidectomy is an accepted treatment of CPH. Long-term effective opening training is crucial in maintaining its therapeutic effect.

Objective To investigate how mutation of nuclear autoantigenic sperm protein (NASP) gene affects mouse liver fibrosis induced by concanavalinA (ConA) and its mechanism. Methods The wild-type B6 (B6-WT) mice were used as a control group, and the NASP mutant B6 (B6-NASP M ) mice as an experimental group. The mice were injected with ConA via tail vein once a week for 8 weeks to establish the model of liver fibrosis. The histopathological changes were observed by HE staining, the collagen fiber deposition by Masson staining, smooth muscle alpha-actin (α-SMA) expression in liver tissue by immunohistochemical staining. The levels of serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) were measured by microplate assay. The serum tumor necrosis factor-alpha (TNF-α) and interferon-gamma (IFN-γ) were measured by ELISA. The mRNA expressions of type I collagen (Col1) and Col3 in liver tissue were determined by real-time quantitative PCR, and the changes of T lymphocyte subsets in liver tissue were detected by flow cytometry. Results Compared with the B6-WT group, B6-NASP M group had disordered liver structure and no significant changes in mRNA expression of Col 1 and Col3, but the collagen fiber hyperplasia and α-SMA expression in liver tissue were more obvious, and the levels of serum ALT and TNF-α were significantly increased. In addition, the proportion and number of CD4 + CD44 hi T lymphocyte subsets in liver tissue were markedly decreased. Conclusion Mutation of NASP gene aggravates mouse liver fibrosis by increasing the release of TNF-α, changing the proportion of T lymphocyte subsets in liver tissue and promoting the activation of hepatic stellate cells.

An in vitro synthesized random ssDNA library was subjected to 12 rounds of selection against anti-screening cells and sieving cells by SELEX. Normal and inflammatory cervical exfoliation cells were selected as anti-screening cells, and the cervical exfoliation cells of low-grade squamous intraepithelial lesion (CIN1), high-grade squamous intraepithelial lesion (CIN2, CIN3) and cervical carcinoma were selected as sieving cells during the screening process. Then, the highly specific aptamer CIN-Ap4 was established by the analysis of the specificity, affinity and cell immunofluorescence, which can be used as biomarker for Cervical Intraepithelial Neoplasia. Prime Premier 5.0 was applied to design a random ssDNA library. According to the fixed sequence at both ends of the library, a pair of primers were designed and synthesized. At the same time, the optimal annealing temperature, cycle times and primer concentration ratio of PCR procedure were selected. The results under the optimal condition are shown as follows. In the 50 μL reaction system, the optimum reaction conditions of symmetry PCR are as follows: annealing temperature is 49.5 ℃, number of cycles is 15. The optimal reaction conditions of indirect asymmetric PCR are as follows: the primer concentration ratio is 80:1, and the number of cycles is 35. The experiment proves that the oligonucleotide library is constructed successfully, and the highly specific dsDNA and ssDNA can be obtained under optimal PCR conditions with good repeatability, which establishes the foundation for the further exploration and experimentation.

Objective: To assess the association of prostasin gene rs12597511 polymorphism with clinical features and pregnancy outcomes among patients with severe preeclampsia., Methods: Clinical manifestations, pregnancy outcomes and the genotypes of 179 patients with severe preeclampsia [early-onset group (≤34 gestational weeks): 79 cases; Late-onset group (>34 gestational weeks): 100 cases] and 222 normal-term pregnant women (control group) were collected., Results: In the early-onset group, the patients with TC or CC genotype at rs12597511 had higher incidences of total complications, liver dysfunction, neonatal asphyxia, neonatal intracranial hemorrhage and perinatal mortality compared with those with TT genotype (P>0.05). Multiple logistic regression analysis showed that the complication rates of severe preeclampsia patients are closely related to TC or CC genotypes, 24 h urinary protein and gestational weeks of onset (OR=1.049, 95% CI:1.007-1.093, P=0.021; OR=1.031, 95% CI: 0.350-0.883, P=0.013; OR=0.733, 95% CI: 0.566-0.950, P=0.019), and the perinatal mortality is related to gestational weeks at delivery (OR=0.542, 95% CI: 0.331-0.887, P=0.015)., Conclusion: Polymorphism of the prostasin gene is closely associated with poor pregnancy outcomes of early-onset severe preeclampsia.

A method was developed for the determination of bisphenol A (BPA) in toys and food contact materials. The BPA was extracted with Soxhlet extraction method from the sample and reacted with acetic anhydride. The final product was determined by gas chromatography-mass spectrometry (GC-MS). To achieve the optimum derivatization performance, the derivatization time and dosage of derivatization reagent etc. were investigated. Under the optimized experimental conditions, the final product was stable and the peak shape was good. The linearity of the derivative was good in the range of 0.05 to 50 mg/L with the correlation coefficient (r2) above 0.999. The recoveries ranged from 80% to 93% at the spiked levels of 0.05, 1.00, 10.00 mg/kg with the relative standard deviations (RSDs) less than 3.7%. The limit of detection (S/N = 3) was 10 microg/kg. The method is accurate and has high recovery. The method is suitable for the inspection of bisphenol A in toys and food contact materials.

To evaluate the acute lung toxicity of intratracheally instilled nano-TiO2 in Kunming mice, healthy adult male Kunming mice were randomly grouped by their body weight (5 mice in each group). The lungs of mice were intratracheally instilled with 1 or 10 mg/kg x bw of nano-TiO2. The control group was intratracheally instilled with the same volume of physiological saline. After 1 d, 7 d, 14 d and 28 d of exposure, the bronchoalveolar lavage fluid (BALF) and lung tissue were collected. The indices of BALF were examined. Lung tissues were assess histopathologically. The results showed that all indices of 10 mg/kg x bw groups were obviously higher than those of the control group and the group of nano-1 mg/kg x bw, respectively. Activities of lactate dehydrogenase (LDH) on the 1st, 7th, 14th and 28th day post-exposure (pe), the amounts of malodialdehyde (MDA) on the 1st, 7th and, 14th day pe and total protein (TP) on the 1st and 7th day pe as well as the amounts of leukocyte on the 1st and 7th day pe of 10 mg/kg x bw groups were significantly different as compared with controls (P < 0.05). There were no obvious changes observed in the activity of alkaline phosphatase (ALP) within groups (P > 0.05). Histopathological examination revealed that the lungs of 10 mg/kg x bw groups presented marked increase in pulmonary inflammation. Many TiO2 particles were still clearly found in the interstitium at 28 days pe. In contrast, low-dose instillation put forward a low risk potential for producing adverse effects on pulmonary health. We conclude that the inflammatory reaction gradually ceased after 28 days. Under the same experimental condition, the effect of lung injury was severer in high-dose nano-TiO2 than in low-dose nano-TiO2.

Background: It has been proven that radiotherapy can induce tumor cells over expressing HIF-1. The aim of this work is to investigate the effect of endostatin and radiotherapy on human lung cancer cell line A549 and the impact of HIF-1 expression after therapy., Methods: Analysed the cell proliferation periods of the A549 cells after treated by endostatin. The A549 cells were treated with endostatin (ES) and/or radiotherpy (RT), and then the cell growth inhibiting rate was detected by methyl thiazolyl tetrazolium (MTT), the expression of HIF-1 was determined by enzyme linked immunosorbent assay (Elisa)., Results: Every treatments can inhibit the growth of A549 cells, but the cells treated with endostatin and radiotherapy were inhibited more obviously. Endostatin had the function of block cell periods on A549, it stoped the A549 cells at G2/M and S periods. Treatment of endostatin and radiotherapy can reduce the expression of HIF-1 induced by radiotherapy., Conclusions: Endostatin was synergistical with the effect of radiotherapy on inhibiting the growth rate of the A549 cells. This synergism may come from: endostatin can make A549 cells accumulated in G2-M and S transition, thereby inhibiting the proliferation of the A549 cells. HIF-1 is a important factor in resistant of cemotherapy and radiotherapy. Endostatin can inhibite the producing of HIF-1 by cells itself or induced by radiation, so increasing the treatment effect of radiotherapy.