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To study the efficacy and compliance analysis of pollen allergen drops in the treatment of allergic rhinitis. The method of single-center controlled was used to analyze the dates' results. From July 2021 to September 2021, 80 patients with seasonal allergic rhinitis were referred to the clinic of otorhinolaryngology in First Hospital of Shanxi Medical University.40 patients received sublingual immunotherapy (SLIT group), and the other 40 patients received symptomatic drug treatment as the control group. The total rhinoconjunctivitis symptom score (TRSS), the visual analogue scale(VAS), total medication score (TMS) and combined scores of medication and rhinoconjunctivitis symptoms (CSMRS) of the patient before the start of the treatment and after the first year of the treatment were compared to assess the efficacy of sublingual immunotherapy of Artemisia pollen. Follow the shedding during the study, the safety of the drug and the causes for compliance analysis were analyzed and recorded. The results of comparison with TRSS, VAS, TMS and CSMRS in two groups in the period of pretherapy were as follows: TRSS(12.393±3.023, 12.450±3.029, t =-0.077, P =0.939), VAS(8.357±1.026, 8.400±0.982, t =-0.173, P =0.862), TMS(3.214±0.568, 3.175±0.501, t =0.301, P =0.764), CSMRS (5.286±0.680, 5.253±0.677, t =0.199, P =0.843), there was no significant difference ( P >0.05); lower observed symptom scores were got in the post-treatment pollen peak SLIT group compared to the control group, TRSS(3.964±1.551, 7.750±2.169, t =-7.918, P<0.05), VAS(2.893±0.956, 5.175±1.481, t =-8.286, P <0.05), TMS (1.821±0.863, 3.175±0.501, t =-8.163, P <0.05), CSMRS (2.489±0.921, 4.468±0.601, t =-10.723, P <0.05), and the differences between the groups were statistically significant ( P <0.05); the SLIT group significantly reduced all symptom scores at the first peak compared to the starting, TRSS(12.393±3.023, 3.964±1.551, t =20.576, P <0.05), VAS (8.357±1.026, 2.893±0.956, t =30.070, P <0.05), TMS (3.214±0.568, 1.821±0.863, t =7.151, P <0.05), CSMRS(5.286±0.680, 2.489±0.921, t =14.533, P <0.05) and there was statistical difference ( P <0.05). No significant adverse reactions occured during medication in the SLIT group. A total of 12 cases were shed in the SLIT group, so the compliance rate was 70%. The four reasons were that patients considered the course was long (4 cases, 33%); the drugs were expensive (3 cases, 25%); patients were busy with their work and life (3 cases, 25%); patients were affected by the outbreak (2 cases, 17%). In summary, Artemisia pollen sublingual drops may improve the symptoms of the patients who got allergic rhinitis caused by Artemisia pollen after the treatment for one year. However, due to the lack of sufficient understanding of immunotherapy or the difficulty in adhering to standardized medication, the compliance with sublingual immunotherapy is still poor, the compliance with sublingual immunotherapy needs to be further improved through patient education.

In the process of orthodontic tooth movement, the secretion of cytokines by immune cells or cell-cell interaction affects the regulation of osteoclast and osteoblast differentiation. Increasingly, studies have focused on the role in the immune system in orthodontic bone remodeling. Based on the biological role of different immune cells or cytokines, this article briefly presents the research progress of immunomodulation in orthodontic tooth movement and future perspective, hopefully providing a deeper and more comprehensive understanding of the biological mechanism in orthodontic tooth movement.

Objective: To study the spatial-temporal dynamical features of hand-foot-mouth disease (HFMD) in Shaanxi Province, so as to provide evidence for the development of relative prevention and control programs on HFMD. Methods: Surveillance data of HFMD was collected from the China Information System for Diseases Control and Prevention from 2009 to 2013. Related data on population and gross domestic product (GDP) was obtained from Shaanxi Statistical Yearbook. Statistical tools as R3.4.1, ArcGIS 10.2 and SaTScan 9.2 software were used to describe the spatial-temporal distribution of the disease. Power-law method on spatial-temporal-multicomponent model was used to analyze the spatial-temporal evolution of the HFMD epidemics in Shaanxi Province. Results: A total of 229 904 cases of HFMD were reported in Shaanxi Province from 2009 to 2013, with an average annual incidence as 122.50 per 100 000. Obvious seasonal characteristics were noticed, with 71.71 % of the total cases identified between April and July. Counties with high incidence were mainly distributed in the mid-and east parts of Guanzhong area. Through temporal and spatial scan statistics, we identified that ClassⅠ clustering area was fixed to the central and southeast regions of Shaanxi province which were around Xi'an City between 2009 and 2013, with the relative risk ( RR ) as 2.24, ranging from 2.18 to 3.08. Results from Power-law analysis showed that the continuous follow-up impact from the previous HFMD epidemics appeared strong in Tongguan, Pucheng districts of Weinan City and Weiyang district of Xi'an, with autoregressive components as 1.14, 0.97 and 0.89, respectively. The risk of HFMD seemed high in Huayin city, Changan and Yanta districts and with the endemic components as 5.08, 4.12 and 4.08, respectively. Impact of the epidemics on nearby districts was largely seen in Lianhu district of Xi'an, Wugong district of Xianyang and Gaoling district of Xi'an with epidemic components as 2.12, 2.08 and 1.77, respectively. The etiological constituents of HFMD were mainly Enterovirus 71 between 2009 and 2012, while HFMD was mainly caused by other entero-viruses, in 2013. Conclusion: Significant spatial-temporal heterogeneity of HFMD was seen in Shaanxi province, which called for specific strategies to be developed in the highly endemic areas.

Objective: To analyze the clinical characteristics of patients with idiopathic right ventricular outflow tract (RVOT) ventricular arrhythmias (VA) and factors related to the immediate success rate of radiofrequency ablation. Methods: Patients diagnosed as idiopathic RVOT arrhythmia in Fuwai Hospital from February 2009 to January 2013 were retrospectively screened. Patients with structural heart disease or inherited arrhythmia were excluded. All patients underwent endocardial electrophysiological study and radiofrequency catheter ablation. Baseline clinical and operation records were collected and analyzed. Immediate success rate was defined as no inducible ventricular arrhythmia by isoprinosine and electrophysiological induction at the end of ablation. The origins of idiopathic RVOT were classified as septal, anterior, posterior, free wall site, epicardial and RVOT-aorta root site. Results: A total of 468 patients were finally included, and the age was (40.4±13.3) years old and 60.5%(283/468) patients were female. Immediate radiofrequency success rate was 89.3%(418/468). Patients were divided into ablation success group ( n= 418) and ablation failure group ( n= 50). Percent of female patients and patients with interventricular septal origin was significantly higher in the ablation success group than in ablation failure group (261(62.4%) vs. 22 (44.0%) , P= 0.01, and 233(55.7%) vs. 18(36.0%), P= 0.005), while percent of patients with epicardial origin was significantly lower in the ablation success group than in ablation failure group (17(4.1%) vs. 11(22.0%), P< 0.001). Immediate success rate was the highest for patients with the septal origin and the lowest for patients with epicardial origin (92.8%(233/251) vs. 60.7%(17/28), P< 0.05). Multivariate analysis showed that the origin site of VAs was the most important independent factor related to the success rate of ablation. Compared with the septal origin patients, patients with RVOT-aorta root and epicardial origin VAs faced with 1.82-fold and 8.26-fold increased risk of failed ablation, respectively ( OR= 2.82, 95% CI 1.05-7.57, and OR= 9.26, 95% CI 3.60-23.86). Sex category was not the independent risk factor for failed ablation( OR =1.76, 95% CI 0.93-3.33, P =0.08) . Conclusions: The immediate success rate of radiofrequency catheter ablation for idiopathic RVOT ventricular arrhythmia is relative high, however, immediate success rate of radiofrequency catheter ablation is relatively low for patients with epicardial and RVOT-aorta root origin arrhythmia and VAs origin is an independent risk factor of immediate ablation success rate.

Objective: To observe the auxiliary curative effect of combined clostridium and bifidobacterium capsules, live (also called Changlekang) in the treatment of allergic rhinitis by comparing IL-10, transforming growth factor-β1(TGF-β1), the quality of life score and symptom score before and after oral Changlekang. Method: Twenty qualified patients in the study group and 20 qualified patients in the control group were enrolled.The study group was given changlekang, desloratadine citrate disodium tablets and mometasone furoate nasal spray for treatment. After 2 weeks of treatment, Changlekang was still given for maintenance therapy for 6 weeks, and no other two kinds of drugs. The control group was given the desloratadine citrate disodium tablets and mometasone furoate nasal spray for 2 weeks and then quited the clinical trials. The changes of symptom score, quality of life score, serum IL-10, serum TGF-β1 would be statistically analyzed during the treatment. Result: ①Before treatment, serum TGF-β1 and IL-10 of normal group was significantly different from the study group and the control group ( P < 0.001), and the study group and the control group's were significantly lower than the normal group.②With the increase of treatment time, serum IL-10, TGF-β1 levels of patients in the study group increased gradually. Four point method ocular symptoms score, VAS ocular symptoms score and total score of RQLQ decreased gradually; ③patients in the control group after 2 weeks'treatment, serum IL-10, TGF-β1 was higher than that before treatment ( P < 0.001), at the same time all symptom scores, total score of RQLQ were lower than those before treatment ( P < 0.001). ④After 2 weeks' treatment, patients in the study group, the serum IL-10 is higher than that of control group ( P < 0.001); ⑤Before treatment serum IL-10, TGF-β1 of AR patients might have little correlation with symptom scores, total score of RQLQ on linear ( P > 0.05). Conclusion: ①serum IL-10, TGF-β1 levels of AR patients may be lower thannormal, and IL-10, TGF-β1 may be involved in the pathogenesis of AR. ②Changlekang may increase the production of serum IL-10, TGF-β1 in AR patients. ③AR patients with desloratadine citrate and mometasone furoate nasal spray treatment combined with Canglekang may promote higher serum IL-10 production than conventional two combination therapy, but there is no more significant improvement in symptoms score and quality of life score. ④Changlekang may improve ocular symptoms and the quality of life in AR patients. ⑤Before treatment serum IL-10, TGF-β1 of AR patients may have little correlation with symptom scores, total score of RQLQ, as a result the level of serum IL-10 and TGF-β1 can not be used to evaluate the severity of AR for the time being., Competing Interests: The authors of this article and the planning committee members and staff have no relevant financial relationships with commercial interests to disclose., (Copyright© by the Editorial Department of Journal of Clinical Otorhinolaryngology Head and Neck Surgery.)

Objective: To evaluate the MRI in the differential diagnosis of acute non-traumatic vertebral collapse., Methods: Sixty-eight cases were retrospected. All cases were studied with sagittal T1-weighted MR images, T2-weighted MR images, gadolinium-enhanced T1-weighted MR images, and axial T1-weighted MR images. Twelve of 68 were performed extra coronal T1-weighted MR images and 22 with additional axial gadolinium-enhanced T1-weighted images., Results: The causes of vertebral collapse were: (1) osteoporosis (n = 31), their images showed retropulsion of bony fragments of collapsed vertebral body which kept the normal signal into the spinal canal as the distinctive sign; (2) metastatic tumor (n = 30), their images showed destructions of pedicles of vertebral and epidural mass as the distinctive sign, and hyperintense or mixed hyperintense signal on T2WI and enhanced T1WI of compressed vertebrae; and (3) multiple myeloma (n = 7), their images showed long T1WI and long T2WI, without destruction of pedicle and epidural mass in compressed vertebrae., Conclusion: MRI is useful to the differentiations of the non-traumatic vertebral collapse in aged.

The effects of DSPM-Cl on ECG in rats, on the dose-effect curve in guinea pig left atria and on the fast action potential (AP), high-K+ depolarized slow action potential (SAP) in guinea pigs papillary muscle were examined electrophysiologically. DSPM-Cl (2 mg.kg-1) showed significant nagative frequency, negative conductivity effect, and prolonged the PP and PR interval. DSPM-CI (30-50 mumol.L-1) was shown to inhibit left atria contractility and shift the concentration-response curve of Iso and CaCl2 to the right with PD2' values of 4.60 and 4.13, respectively. In addition, DSPM-Cl was found to prolong the duration of action potential 90 (APD90) and effective refractory period (ERP), and decrease the maximal upstroke velocity (Vmax) in K(+)-depolarized guinea pigs papillary muscles. The results suggest that, like verpamil, DSPM-Cl might be a calcium antagonist.

A reversed phase high performance liquid chromatography (RP-HPLC) method for determination of 2-(p-dimethylaminostyryl) pyridine methiodide (DSPM) concentration in rabbit plasma is reported. The stationary phase was YWG-C18H37. The mobile phase consisted of 80% CH3OH, 20% CH3COOH-CH3COONa buffer (pH 4.0, 0.20 mol/L) and (C2H5)2NH 25 mmol/L. Its flow rate was 1.0 ml/min. The column temperature and pressure was 28 degrees C and 10.13 MPa, respectively. The detect wavelength was 460 nm. The detection limit of DSPM was 2 ng or 0.08 mumol/L in plasma. The RSD (%) of within-day and between-days were less than 7%. A good linear relationship between peak area and DSPM concentration in plasma (0.68-13.65 mumol/L) was found (r = 0.9996).

Mice were injected ip DSPM 1 or 3 mg.kg-1 3 h prior to a subcutaneous injection of isoproterenol (Iso) 20 mg.kg-1 once daily for 2 d. Iso induced reductions of Se-glutathione peroxidase (Se-GSH-PX), superoxide dismutase (SOD) activities, and an increase of malondialdehyde (MDA) content in myocardium. DSPM 1 or 3 mg.kg-1 significantly abated reduction of Se-GSH-PX activity and decreased MDA production and DSPM 3 mg.kg-1 also abated reduction of SOD activity in the hearts from Iso-treated mice. The changes of above indices were in accordance with those of myocardial ultrastructure and creatine phosphokinase (CPK) concentration in serum. The results indicate that DSPM has a protective effect on myocardial ischemic injury probably by inhibiting oxygen free radicals and subsequent lipid peroxidation.

Isoproterenol (Iso, 20 mg.kg-1 x d-1 x 2 d) induced widespread and severe myocardial damages at ultrastructural level, decreased the myocardial Se-glutathione peroxidase (Se-GSH-Px) and superoxide dismutase (SOD) activities, increased the serum creatine phosphokinase (CPK) concentration and myocardial malondialdehyde (MDA) content. Phenytoin sodium (Phe) 15 or 30 mg.kg-1 ip pretreatment diminished the CPK release and MDA production, protected the Se-GSH-Px activity in the Iso-induced damage of mouse heart. The pretreatment with 30 mg.kg-1 abated the reduction of SOD activity. However, Phe 15 mg.kg-1 did not show such an effect. Phe (15 or 30 mg.kg-1) reduced the ultrastructural cardiotoxicity of Iso, and the membrane structure of ischemic myocardium was protected. The protective effects of verapamil pretreatment 3 mg.kg-1 ip were weaker than those of Phe on ultrastructural changes, but biochemical changes were similar to those of Phe. The results suggested that Phe possessed anti-lipid peroxidation and protective effects on ischemic myocardium.

The effects of 2-[p-(dimethylamino)styryl] pyridine methiodide (DSPM) on rat ECG and HBE were studied. The P-R and R-R intervals of ECG were lengthened 40.3% and 115.4% respectively, after iv injection of DSPM 2 mg/kg. DSPM (1.5 and 2 mg/kg) affected the A-H interval in a dose-dependent manner with no change in the H-V interval of HBE. DSPM 0.5 mumol/L lengthened the A-H interval of isolated perfused hearts in frequency-dependent manner, with greater prolongation at higher frequency. The effect of verapamil on the A-H interval was less than that of DSPM. Propranolol had no effect on the frequency-dependent delay of atrioventricular node. DSPM 0.5 mumol/L lengthened the functional and effective refractory periods of atrioventricular node by 26.8% and 31% respectively. These phenomena support that DSPM may be a calcium antagonist.

ip DSPM 5 mg/kg 1 or 4 h before excision of the heart was found to prevent damage to the heart due to global myocardial ischemia. Compared with the controls, the heart rate, coronary resistance, contractile force and edema of the heart were all greatly improved during the 60 min reflow after 25 min of no-flow, especially the heart pretreated with DSPM 4 h prior to excision. In addition, DSPM 1.5, 6 mg/kg markedly reduced the myocardial Ca2+ content measured by isoproterenol in mice. DSPM at higher dosage also lowered the normal myocardial Ca2+ content. These results suggest that DSPM may have a protective effect on myocardial ischemia which might be associated with its calcium antagonistic action.

The effects of 2-[p-(dimethylamino) styryl]pyridine methiodide (DSPM) on rabbit aortic contraction induced by high K+, norepinephrine (NE) and Ca2+ were studied. On rabbit thoracic aortic strips, DSPM inhibited the contraction induced by KCl and NE in normal modified Tyrode's solution. DSPM shifted the dose-response relationships of KCl and NE to the right, and depressed their maximal responses; the pD'2 values were 5.09 and 5.16 respectively. After the strip had been exposed to Ca2(+)-free Tyrode's solution for 2 h, the NE-induced responses were further inhibited by DSPM, whereas the KCl-induced responses were not significantly affected. On depolarized vessels, the dose-response relationships of CaCl2 were also shifted to the right and their maximal responses were also depressed by DSPM, suggesting that DSPM is a non-competitive antagonist. The pD'2 value was 6.86. These results suggest that DSPM is a calcium antagonist. It not only inhibited the influx of extracellular calcium, but also has an inhibitory effect on the release of sequestered calcium in the rabbit aortic smooth muscle.

The effects of 2[p-(dimethyl-amino)styryl]pyridine methiodide (DSPM) on the action potential of dominant pacemaker cells in sinoatrial nodes of rabbits were studied by microelectrode technique. DSPM 45 mumol/L decreased the slope of phase 4, the maximum rate of rise of the action potential and the action potential amplitude by 54% (P less than 0.01), 27% (P less than 0.05) and 13% (P less than 0.05), respectively, and increased the spontaneous sinus cycle length and action potential duration at 90% repolarization by 62 and 26% (P less than 0.01), respectively. These effects of DSPM were similar to that of nifedipine 3.9 mumol/L. The positive chronotropic effect of nicotinamide 33 mmol/L was completedly blocked by DSPM 45 mumol/L while that of isoprenaline 0.11 mumol/L was partially blocked by DSPM 45 mumol/L. The results suggest that DSPM may be a Ca2+ antagonist.

Effects of 2-[p-(dimethylamino) styryl] pyridine methiodide (DSPM), nifedipine (Nif), CaCl2 and isoprenaline (Iso) on action potentials (AP) of guinea pig papillary muscle cells depolarized by high K+ (15.4 mmol/L) were studied by microelectrode technique. DSPM 45 mumols/L decreased the Vmaxf and Vmaxs of phasee O of the AP by 14 and 26%, respectively. APD90 was lengthened by 7%, amplitude of action potentials (APA) was decreased by 3%. The effects of CaCl2 on Vmaxs of phase 0 of the AP of depolarized papillary muscle cells were abolished by DSPM 45 mumols/L, but the effects of Iso were not antagonized. Effects of DSPM on Vmaxs of the AP of depolarized papillary muscle cells and the influence of DSPM to the action of CaCl2 and Iso were similar to those of Nif. It further suggests that DSPM may be a calcium antagonist.

The effects of 2[p-(dimethylamino)styryl] pyridine methiodide on mouse and rabbit ECG and on the contraction of isolated rabbit atrial muscles were studied. DSPM produced long-lasting bradycardia and A-V block in a dose-dependent manner, but did not effect intraventricular conduction. DSPM antagonized the positive chronotropic and dromotropic effects of isoprenaline (Iso) in vivo, and antagonized the inotropic effects of Iso and CaCl2 non-competitively in vitro. The pD'2 were 4.49 and 4.52, respectively. It is suggested that DSPM may be a Ca2+ antagonist.