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Objective To evaluate the impacts of equal ratio ventilation (ERV) mode on lung morphology, gastric inflation and laryngeal mask airway ventilation during laparoscopic radical prostatectomy by lung ultrasound（LUS） and gastric ultrasound. Methods A prospective study was performed in 60 patients with prostate cancer undergoing laparoscopy from June 2021 to May 2022 in Nanjing First Hospital. The patients were randomly divided into ERP group (group E) and conventional ventilation group (group C,n=30, each). After induction of routine intravenous anesthesia and mechanical ventilation with laryngeal mask airway, and the ventilation mode was set as follows: the inspiratory-to-expiratory ratio in group E was 1∶1, while that in group C was 1∶2. After anesthesia induction (T0), 30 minutes after surgery begin (T1), 60 minutes after surgery begin (T2) and 10 minutes after surgery (T3), the peak airway pressure (Ppeak), airway plateau pressure(Pplat), mean airway pressure (Pmean),end-expiratory partial pressure of carbon dioxide (PETCO2) and minute ventilation (MV) were observed and compared between two groups. The LUS score, the cross-sectional area of gastric antrum and fundus and the incidence of airway complications were recorded in two groups. Results At T1 and T2, Ppeak in group E was significantly lower than that in group C(P＜0.05), and Pmean was significantly higher than that in group C(P＜0.05). There was no significant difference in PETCO2 and MV between two groups at different time points (P＞0.05). The LUS and the incidence of atelectasis in group E were statistically lower than those in group C (P＜0.05), and there was no significant difference in the cross-sectional area of gastric antrum and fundus（P＞0.05） and the incidence of airway complications（30.0% vs 32.1%，χ2=0.031，P=0.860) between two groups. Conclusion ERV mode during laparoscopic radical prostatectomy can reduce Ppeak, leakage rate of laryngeal mask, atelectasis and LUS scores without increasing the incidence of gastric inflation and laryngeal mask-related adverse complications.

Short for thin seam inclined wall face support, a semi-thin coal unloading alternating push-powered support was presented, the stent including the hand pump system and support system two parts. Manual pump system uses novel multi-piston pump to the manual hand pump to maximize flow, support system using split roof beams and base frame is arranged around a hand pump system control, manual pump oil back into use with a single stent since the rapid valve closure adapters, plug convenience, in the support system is in state support, multi-directional plunger pump system can be placed in a secure location; support system to meet the telescopic multi low cutting height requirements, before and after to display vertical half-frame, semi-uninstall alternate along the strike forward, manually complete the short wall of thin supporting roof work. [ABSTRACT FROM AUTHOR]

This study focused on four vegetation restoration stages of grasslands, shrublands, secondary forests and primary forests in the typical karst peak-cluster depression. The soil core method was used to collect fine roots with 2 mm or less in diameter in three layers (0-10, 10-20, 20-30 cm). The biomass, morphological characteristics of fine roots and their relationship with soil properties were analyzed. The results showed that fine root biomass ranged between 194.63 and 255.19 g·cm -2 in different vegetation restoration stages. Most of fine roots distributed in the surface soil of 0-10 cm, which accounted for more than 60% of the total biomass in the soil layer of 0-30 cm. No significant difference was found among different stages in fine root biomass. There was significant difference among different stages in the specific root length and specific surface area of fine roots. Both parameters were gradually decreased with vegetation forward restoration from grassland to primary forest. More than 66% root length and 64% root area were distributed in the surface soil of 0-10 cm. The length and area of most of the fine root were in the diameter class of 0-0.5 mm and 0.5-1mm, respectively. These two levels of the root length and root area accounted for more than 87% and 72% of the total amount, respectively. Results from the redundancy analysis showed that there were different correlations between karst peak-cluster depression vegetation community characteristics and soil properties, with soil organic carbon, available potassium, and total nitrogen having a great influence on the characteristics of fine roots. It is an effective strategy for plants to better adapt to the habitats.

Objective: To explore the surgical treatment of paraclinoid aneurysms and evaluate the safety and efficacy of microsurgical clip and endovascular embolization of paraclinoid aneurysms., Methods: The data of 28 patients with 30 paraclinoid aneurysms receiving surgical treatment were retrospectively analyzed. According to Barami classification, 4 aneurysms were type Ia, 5 aneurysms type Ib, 13 aneurysms type II, 4 aneurysms type IIIa, 1 aneurysm type IIIb, and 3 aneurysms type IV. In the study, 15 cases with 17 paraclinoid aneurysms received microsurgical treatment, of which 3 cases underwent superficial temporal artery-middle cerebral artery (STA-MCA) bypass firstly and then aneurysms were trapped. The other 13 cases received endovascular embolism, in which balloon assistant technology was performed in 3 cases and stent assistant technology in another 3 cases., Results: All the 30 paraclinoid aneurysms were treated. One patient with bilateral paraclinoid aneurysms became blind after bilateral microsurgical procedures and another patient was unexceptedly dead 10 d after operation. Vasospasm resulting with cerebral infarction occurred in one case who received endovascular embolism, while two patients suffered from hydrocephalus. Ten cases of microsurgical group and 9 cases of interventional group were followed up with digital subtraction angiography, which disclosed that 9 cases of microsurgical group were clipped completely, while 7 aneurysms of interventional group were completely embolised. One aneurysm recurred and enlarged 12 months after stent assistant embolism, and after STA-MCA bypass and bilateral anterior cerebral artery anastomy and aneurysm insulation, the patient recovered well. When the patients were discharged, their Glasgow outcome scales showed that 18 cases were with 5, 8 with 4, 1 with 3 and 1 with 0., Conclusion: According to the classification, and with appropriate treatment, the patients with paraclinoid aneurysms will get good outcomes both with microsurgical clipping and with endovascular embolization.

Objective: To explore the effect of superficial temporal artery to middle cerebral artery(STA-MCA) bypass in treatment of cerebrovascular disease., Methods: Fifty-two patients with atherosclerotic carotid artery occlusion or middle cerebral artery occlusion or moyamoya disease were included in this study. There were both clinical ischemic manifestation and hemodynamic dysfunction detected by perfusion CT in all the patients. DSA and perfusion CT (PCT) were conducted before and after STA-MCA bypass in order to evaluate the change of hemodynamics and the state of anastomotic astium. The patients were followed-up to know if there was any complication or recurrent stroke. Modified Rankin scale (mRS) was used in assessing the state of neurological function., Results: STA-MCA bypass was performed successfully in 50 patients, while anastomotic astium was found to be obstructed in 2 patients during operation and temporalis attachment was conducted immediately. Complications occurred in 4 patients, 2 with subdural hematoma, 1 with hematoma in contralateral basal ganglia, and 1 with poor wound healing. Postoperative DSA showed that anastomotic astium was opened well in 48 patients, while PCT displayed a significant improvement in both relative cerebral blood flow [rCBF,(37.79±9.76)mL/(min×100 g)vs.( 33.71±7.92)mL/(min×100 g),P<0.05] and the relative mean transmit time [rMTT,(8.49±1.97)s vs. (11.06±3.00)s,P<0.01]. mRS improved significantly in both the 3 month and 12 month follow-ups(1.37±0.66 vs. 1.58±0.64, 0.84±0.57 vs. 1.51±0.67,P<0.05). There was no ipsilateral stroke during the follow-up., Conclusion: STA-MCA bypass may improve the hemodynamic and neurological condition and prevent the recurrence of ischemic stroke in patients combined with occlusive cerebrovascular disease and hemodynamic disturbance.

Meningomyelocele combined with squamous cell carcinoma is rare in literature. In this article, we report the clinical and treatment of a patient with meningomyelocele and squamous cell carcinoma and discuss its mechanism, clinical feature, therapy and prognosis.The patient was a 11-year-old Chinese boy.At the time of his birth he was noted to have a lumbosacral meningomyelocele, which was disrupted and the cerebral spinal fluid flew out when the child was six.The wound surface abrased and exudated repeatedly.Two months before admission,the meningomyelocele was disrupted again and the condition got worse.Inspection showed a meningomyelocele in the lower lumbar region 10 cm in diameter,consisting of a cauliflower-shaped swelling and a central crater containing black slough.The area smelled foul and was constantly draining serosanguineous fluid.Magnetic resonance imaging showed meningomyelocele associated with spinal dysraphism and tethered cord syndrome.After thorough preparation, operation was undertaken.A perpendicular skin incision,which was carried down to the lumbar aponeurosis,allowed the main bulk of the tumour to be undercut and removed.The quick frozen pathological examination confirmed that it was squamous cell carcinoma.The skin and subcutaneous tissue were further resected and the vertebral canal explored until frozen section showed the excision edge was clear.Skin closure was achieved by a bipedicle advancement flap,some 10 cm wide and the secondary defect was closed with a thigh skin graft.Histological examination showed that the massive outgrowth was a well-differentiated squamous cell carcinoma.The postoperative recovery was uneventful and the wounds healed by primary intention.Although meningomyelocele combined with squamous cell carcinoma is rare in literature,the possibility of cancerization should be considered when there is a long-term and non-healing ulcer (Marjolin ulcer) with foul smell in a meningomyelocele patient.

Congenital bilateral perisylvian syndrome (CBPS) is rare in literature, especially in China. In this article, we report the clinical and treatment of a patient with CBPS and discuss its mechanism, clinical features and therapy. This patient was a 28-year-old man. His main clinical features were pseudobulbar palsy, cognitive deficits and intractable epilepsy. MRI shows bilateral thickening of the cortex around the sylvian fissures which were deeper than normal and polymicrogyria. The electroencephalogram demonstrated slow spike in right temporal lobe and left frontal lobe. Rhythmal 4 Hz theta waves exist in left frontal and parietal lobe. As the epilepsy was poorly controlled by antiepileptic, section of the corpus callosum was carried out. After callosotomy, there was pronounced seizure reduction and intelligence development improvement. CBPS is characterized by pseudobulbar palsy, cognitive deficits, and bilateral perisylvian abnormalities in imaging studies. If intractable epilepsy is combined, callosotomy may be effective.

Aim: To investigate the anti-tumor immune response of lymphocytes elicited by HepG2 cells modified with CD80-IgG1 Fc fragment fusion protein (CD80-Fc)., Methods: HepG2 cells were modified with CD80-Fc, then the expression of CD80 on the cell surface was analyzed by flow cytometry (FCM). After mixed lymphocyte-tumour cell reaction (MLTR) of the modified HepG2 cells and peripheral lymphocytes of healthy volunteers, the proliferation and cytotoxicity of the lymphocytes were tested by MTT colorimetry and LDH release assay,respectively., Results: CD80-Fc could be efficiently bound on HepG2 cells. HepG2 cells modified with CD80-Fc fusion protein dramatically elicited proliferation and cytotoxicity of normal lymphocytes., Conclusion: CD80 may play an important role in anti-tumour immune response. HepG2 cells modified with CD80-Fc fusion protein can elicited potent anti-tumor immune response. This fusion protein provides a convenient means for further potential use in immunotherapy of tumor.