Search

In order to clarify the optimal planting density of soybean variety Liaodou No.21 in Luoping County, Liaodou No.21 was taken as the experimental material, and five density treatments were set up by using the randomized block design, including Treatment A (20 plants·m-2), Treatment B (25 plants·m-2), Treatment C (30 plants·m-2), Treatment D (35 plants·m-2), and Treatment E (40 plants·m-2). Then, the effects of different planting densities on the growth dynamics, agronomic traits and yield of Liaodou No.21 were studied. The results showed that the planting density had a great influence on the yield of Liaodou No.21. When the planting density was 30 plants·m-2, the total yield reached its maximum value of 2933.6 kg·hm-2. The lower planting density resulted in the insufficient number of soybean populations, while the planting density was too high, it was difficult for the individual plants to obtain sufficient nutrients, energy, and water for their growth. Therefore, an appropriate planting density was crucial for improving the yield of Liaodou No.21. [ABSTRACT FROM AUTHOR]

In order to investigate the feasibility of in vitro screening the antitumor activity of natural compounds by trypsin, porcine trypsin was used to for screening test, which is marked by inhibition of enzyme activity. Four compounds, namely daidzin, genistin, matrine and oxymatrine, were selected as test subjects. The natural antitumor drug camptothecin was used as the control. The inhibitory effect was detected by two experimental methods: direct detection of trypsin activity inhibition and hydrolysis of bovine serum albumin by trypsin. The results showed the inhibitory effects of the four natural compounds on trypsin, and the inhibition rates of the four natural compounds were significantly different. The enzyme activity assay showed that the inhibitory effect of matrine was better than that of oxymatrine, indicating that trypsin had a good screening resolution. The inhibitory effect was significantly increased with the increased ratio of sample to trypsin, suggesting the structure-activity correlation and dose-effect correlation of the screening methods. Altogether, the experimental method of screening antitumor activity of natural compounds by trypsin has good application values. Since porcine trypsin is similar to human trypsin in terms of molecular structure and performance, it is more applicable for screening of antitumor efficacy of natural pharmacodynamic compounds.

Objective: To investigate the curative effect of different internal fixation for ipsilateral fractures of the femoral neck and shaft., Methods: By retrospective study of 27 patients who sustained ipsilateral femoral shaft and neck fractures from June 1993 to March 2004. There were 22 male and 5 female, with an average age of 35 years (range in 14 to 65 years). The femoral neck and shaft fractures were stabilized with dynamic hip screw system (DHS) in 3 cases,with dynamic compression plate and cannulated lag screw in 12 cases, with constructive nail in 8 cases, with antegrade intramedullary locking nail and cannulated lag screw in 4 cases. There were 13 cases used of the temporary fixation of Kirschner wire before the pexia of the femoral neck fractures., Results: All of the patients were followed up for 36 to 75 months, with an average of 44 months. The average healing period of femoral neck fracture was 4.5 months in 25 cases, nonunion of femoral neck fractures in 2. The average healing period of femoral shaft fracture was 6 months in 27 cases. In 14 cases that not using temporary fixation of femoral neck with Kirschner wire, there were nonunion of femoral neck in 2 and slight coxa vara in 3., Conclusion: There are a wide choice of internal fixation method for treatment of ipsilateral fracture of the femoral neck and shaft, the fixation with dynamic compression plate and cannulated lag screw is a handy method. It would be avoided to the replacement and trauma, emporary fixing the fracture with Kirschner wire before the pexia of the femoral neck fracture.

Objective: To investigate the effects of period and area factors of manganese exposure on the postnatal development of brain in rats., Methods: A combination of GFAP cytoimmunochemistry and imaging analysis was used to determine the morphological changes of glial cells and the average proportional densities of GFAP-immunochemistry positive products of different brain areas in different Mn-exposured period., Results: (1) In both one month's and two month's groups, no differences were found in the GFAP-immunochemistry extensities and the average proportional densities of GFAP reaction positive products between the Mn-exposured group and the control group in the somatic motor and sensory cortexes, but in the expyramid nuclei or areas, that were increased significantly than the the control group. (2) In three month's Mn-exposured groups, the GFAP-immunochemistry extensities and the average proportional densities of GFAP reaction positive products in all related areas of three groups were previously increased than that of the control group., Conclusion: In the interferential factors of manganese exposure on the postnatal brain development, both the Mn-exposure period and the areas were the important ones.