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Objective: To investigate the expression of long chain non-coding RNA (LncRNA) colon cancer related transcription factor 1 (CCAT1) and zinc finger binding protein 1 (ZEB1) in lung adenocarcinoma tissues and their relationship with clinicopathological parameters and prognosis of patients. Methods: 138 lung adenocarcinoma patients who were admitted to our hospital from February 2017 to February 2020 were selected. Surgically resected cancer tissues and cancer adjacent tissues (more than 2 cm from the edge of cancer tissues) were taken. The expression of lncRNA CCAT1 and ZEB1 mRNA in cancer tissues and cancer adjacent tissues were detected by real-time fluorescence quantitative polymerase chain reaction. The expression of ZEB1 protein in cancer tissues and cancer adjacent tissues was detected by immunohistochemistry. The correlation between lncRNA CCAT1 and ZEB1 mRNA expression in cancer tissues and clinicopathological parameters of lung adenocarcinoma patients were analyzed. The prognosis of lncRNA CCAT1 (high and low expression) and ZEB1 mRNA (high and low expression) in lung adenocarcinoma patients were analyzed by Kaplan-Meier method. The prognostic factors of lung adenocarcinoma patients were analyzed by multivariate Cox proportional hazard models. Results: The relative expression levels of lncRNA CCAT1 and ZEB1 mRNA in lung adenocarcinoma cancer tissues were higher than those in cancer adjacent tissues, respectively (P<0.05). The relative expression of lncRNA CCAT1 and ZEB1 mRNA in cancer tissues of lung adenocarcinoma patients was positively correlated (r=0.532, P<0.05). The relative expression levels of lncRNA CCAT1 and ZEB1 mRNA in lung adenocarcinoma patients with TNM stage IIIa and lymph node metastasis were higher than those in patients with stage I～II and no lymph node metastasis (P<0.05). The 3-year overall survival cases of lncRNA CCAT1 high expression group and low expression group were 39(60.00) and 65(92.86)respectively. The 3-year overall survival cases of ZEB1 mRNA high expression group and low expression group were 41(62.12) and 63(91.30) respectively. Compared with the lncRNA CCAT1 low expression group and ZEB1 mRNA low expression group. The lncRNA CCAT1 high expression group and ZEB1 mRNA high expression group had lower 3-year overall survival rates (P<0.05). high expression of lncRNA CCAT1, high expression of ZEB1 mRNA and lymph node metastasis were independent risk factors for poor prognosis in lung adenocarcinoma patients (P<0.05). Conclusion: The expression of lncRNA CCAT1 and ZEB1 mRNA in lung adenocarcinoma tissues is up-regulate, the up-regulation of both expression is related to lymph node metastasis, and can lead to poor prognosis, they are expected to become a potential marker for evaluating the prognosis of lung adenocarcinoma patients. [ABSTRACT FROM AUTHOR]

Objective To explore the expression of ZEB1-AS1 in endometrial cancer (EC), and its correlation with clinicopathological features. Methods The expression of ZEB1-AS1 and ZEB1 in 195 pairs of endometrial cancer and paracancerous tissues were detected by qRT-PCR analysis. We analyzed the correlation of ZEB1-AS1 and ZEB1 expression with the clinical characteristics of EC patients. MTT assay, Transwell migration assay and invasion assay were used to detect the proliferation, migration and invasion of EC cell lines after ZEB1-AS1 overexpression. Results ZEB1-AS1 and ZEB1 were upregulated in EC tissues (P < 0.05). The high expression level of ZEB1-AS1 and ZEB1 were positively correlated and could shorten the overall survival time of EC patients (P < 0.05). The high expression levels of ZEB1-AS1 was correlated with family history, pathology grade, pathological type, chemotherapy, clinical stage, T stage, M stage and HPV infection of EC patients (P < 0.05). The high expression levels of ZEB1 was correlated with family history, pathology grade, clinical stage, T stage, N stage and M stage (P < 0.05). Overexpression of ZEB1-AS1 promoted the proliferation, migration and invasion of EC cell lines (all P < 0.05). Conclusion Both ZEB1-AS1 and ZEB1 are upregulated in EC tissues and could promote the metastasis, invasion of endometrial cancer and indicate the poor prognosis.

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