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In order to understand the pyrolysis and explosion characteristics of typical wood fiber dusts, the pyrolysis process, kinetic parameters, and pyrolysis gas types of the two dusts were calculated and analyzed using thermogravimetric-infrared coupler with redwood fiber dust and poplar fiber dust as the research objects. The results showed that the pyrolysis rate of mahogany dust was higher than that of poplar dust, and the activation energy was lower. The thermal weight loss of the two dusts was mainly reflected in the thermal decomposition at low temperature and combustion reaction at high temperature, and the pyrolysis products were mainly CO2, CO, H2O and a small amount of alkanes, alcohols, aldehydes, ketones and carboxylic acids, and the combustion products were mainly CO2 and CO, and the amount of CO2 of combustion products of mahogany fiber was higher than that of poplar fiber, while the pyrolytic combustion process of poplar fiber is relatively complex, with a high degree of incomplete combustion. The dust cloud minimum ignition temperature and maximum explosion pressure of the two wood fiber dusts were tested. Redwood fiber dust was more dangerous than poplar, and the mechanism was explained from the thermogravimetric-infrared test results. The results of this study could provide some data reference for the development of relevant policies and measures at sites involving such dusts. [ABSTRACT FROM AUTHOR]

Neuron is the basic unit of nervous system. Studying the fine morphology of neurons is crucial for understanding the structure of neural network and the manner of neural information processing. At present, effective methods to visualize fine morphology of neurons with specific cell types and specific projection are still lacking. In this paper, a recombination vesicular stomatitis virus (VSV) was designed, with nucleoprotein gene mutated and glycoprotein gene deleted. The virus, VSV-△G-NR7A-EGFP, was capable of highlighted morphology of the labelled neurons in a short time. With specific transgenic animals and a helper virus rAAV-EF1α-Dio-Bfp-Tva, it was demonstrated that, a further constructed virus VSV-△G-EnvA-NR7A-EGFP could sparsely and specifically label D2R neurons with nucleus accumbens-lateral hypothalamus (NAc-LH) projection and dopamine neurons with ventral tegmental area (VTA)-NAc projection.

Quantitative analyses of molecular expression, cell counts and neural network connections among different brain regions are essential in brain science research. Based on Paxinos and Franklin's the Mouse Brain in Stereotaxic Coordinates (Ⅱ) and public image processing software such as Photoshop and ImageJ, we developed a convenient method for semi-automatic segmentation and cell counting on brain sections. A standard template for brain region segmentation was first obtained from the Paxinos and Franklin's mouse brain atlas. Photoshop was then used to transform the standard template semi-automatically into the space of brain sections, yielding masks of segmented brain regions. Finally, ImageJ was used to analyze the data in different brain regions. This method is useful for immunohistochemical and neuron distribution pattern analyses, as well as neural network labelling studies. The method does not require expensive commercial image analysis software, and is also easy to implement and use.

In order to obtain the energy consumption and efficiency data of simple lifts, energy transfer was studied under different working conditions. Comparative testing scheme of energy efficiency was established according to the actual situation. Among energy supply, effective nominal value and the nominal value of energy efficiency, the nominal value of energy efficiency was shose as the index to evaluate the simple lift energy consumption level. Three-phase power quality analyzer was adopted to test the energy efficiency of two different type simple lifts with same parameters. The measured values of energy supply, available energy, energy efficiency and active power were comparative analyzed with the theoretical values, under different working conditions. Finally, test results and analysis show that since there is an energy feedback loop during the falling process for the forced type simple lift, the overall traction type simple lift is more energy-saving. Also, the test results provide the foundation for energy consumption of simple lifts. [ABSTRACT FROM AUTHOR]

Objective: To screen out the suitable erythrocytes compatible to the results from the routine blood matching for the patients suffered from the relapse of acute hemolytic transfusion reaction., Methods: The in vitro hemolysis test was used to screen out the erythrocytes from the non-hemolytic donors for the transfusion of erythrocytes into the patients., Results: Three U of the non-hemolytic erythrocytes were obtained by using hemolytic test in vitro, the post-transfusion effects were good, and the hemolytic reaction will not occure once more., Conclusion: When it is compatible to the results obtained from the routine blood matching and the post-transfusion hemolytic reaction appeared. The blood matching by using in vitro hemolytic test can be used to screen out the non-hemolytic erythrocytes for transfusion of the patients.

The stable hydrogen and oxygen isotopes are environmental isotopes, which widely exist in various kinds of water. Their relative abundance variation in water can indicate the water circulation and mechanism of water use in plant. This research selected two major kinds of greening tree species, evergreen coniferous Platycladus orientalis and deciduous broad-leaved Quercus variabilis, in Beijing mountainous area, and the water movement process in soil-plant-atmosphere continuum was investigated by the variation characteristics analysis of stable hydrogen and oxygen isotope compositions in precipitation, soil water, groundwater, plant stem water and leaf water. The results showed that the meteoric water line equation of the study area was δD=7.17δ 18 O+1.45 (R 2 =0.93), and the soil evaporation line equation was δD=3.85δ 18 O+1.45 (R 2 =0.76). A certain degree of evaporation fractionation existed in the processes of rainfall infiltration into soil water. In different seasons, the δD and δ 18 O values of precipitation, soil water and spring water had different variation regularity. In rainy season, the mean δD and δ 18 O values were in order of precipitation> spring water>soil water, with the precipitation and soil water supplied spring water together; in dry season, the order was precipitation > soil water > spring water, and the precipitation and spring water both contributed to soil water. The δD and δ 18 O fitting line equations of stem water of P. orienta-lis and Q. variabilis were respectively δD=5.03δ 18 O-30.78 and δD=3.0δ 18 O-48.92. The uptake water of Q. variabilis was more enriched than that of P. orientalis, and the depth of Q. variabilis water uptake in soil profile was shallower than P. orientalis. The leaf water isotopic variation of Q. varia-bilis was more sensitive to atmospheric environment, with the kinetic isotopic fractionation of Q. variabilis being more enriched than that of P. orientalis, but they had the same response to variation of environmental condition.

Microbial community and phosphorus forms in response to simulated climate warming were studied by high-throughput sequencing and 31 P nuclear magnetic resonance( 31 P-NMR) respectively, which were from wetland soils in constructed microcosm columns. The results revealed that relative abundances of Firmicutes, Clostridia, Clostridiales, Clostridiaceae and Clostridium were significantly decreased by 65%-98%, 69%-87%, 67%-87%, 73%-97% and 74%-93% under warming condition respectively, suggesting warming had a significant inhibitory effect on the bacterial lineage from Firmicutes to Clostridium at different taxonomic level. Particularly, principal coordinate analysis and cluster analysis also demonstrated warming had a significant effect on microbial community structure with obvious separation of samples between control and warmed groups from each wetland column site. Phosphorus forms were dominated by phosphomonoester and orthophosphate in each wetland column soil, which were significantly increased and decreased by 275% and 20% in XX wetland column soil respectively. Similarly, phosphomonoester and polyphosphate were also found to be increased and decreased by 85% and 49% in JH wetland column soil respectively, indicating that phosphorus forms in response to warming had soil heterogeneity. Canonical correspondence analysis showed that obvious changes in microbial community composition had significant effects on phosphorus forms under warming condition.

PM 2.5 and PM 10 have become the primary pollutants of most cities in China. Atmospheric PM 2.5 and PM 10 mass concentrations, meteorological factors, traffic flow from 2013 to 2015 in Nanchang were analyzed. Spatiotemporal dynamic pattern of atmospheric particulate matter pollution and the effect of weather and traffic on particle concentration change were discussed in this paper. The results showed that PM 2.5 (70.92 μg·m -3 in 2013 > 53.70 μg·m -3 in 2014 ＞ 43.65 μg·m -3 in 2015) and PM 10 (119.72 μg·m -3 in 2013 ＞ 86.11 μg·m -3 in 2014 ＞ 73.32 μg·m -3 in 2015) concentrations decreased gradually from 2013 to 2015. In addition, low concentrations of PM 2.5 and PM 10 in summer (average PM 2.5 concentration 36.74 μg·m -3 , average PM 10 concentration 69.20 μg·m -3 ) but high concentrations in winter (average PM 2.5 concentration 74.29 μg·m -3 , average PM 10 concentration 111.64 μg·m -3 ) were observed. Moreover, PM 2.5 and PM 10 concentrations changed with the urban-rural gradient, decreasing from the city center to suburb. The ratio of PM 2.5 /PM 10 (0.595 ＞ 0.584 ＞ 0.557) decreased year by year from 2013 to 2015 and was higher in the city center area than in the edge of city. PM 2.5 and PM 10 concentrations were affected by various meteorological factors and significantly related to air pressure, temperature, relative humidity, wind speed, precipitation and sunshine time. The influence of meteorological factors differed on PM 2.5 and PM 10 concentrations. Traffic flow significantly increased the surrounding PM 2.5 concentration, but not PM 10 concentration.

Objective: To evaluate the effects of the combination of basic fibroblast growth factor (bFGF), transforming growth factor-Β1 (TGF-Β1), bone marrow mesenchymal stem cells (BMSCs), and temperature-responsive chitosan hydrogel (TCH) gel on the repair of degenerative intervertebral disc in rat models., Methods: Rat models of intervertebral disc degeneration were established by acupuncture. The degenerative effects were observed under magnetic resonance imaging (MRI). The BMSCs was cultured in vitro and then transfected by adenovirus with enhanced green fluorescent protein to make it carry the gene of enhanced green fluorescent protein,which functioned as fluorescence labeling. The SD rat models of intervertebral disc degeneration were divided into four groups: group A, treated with the combination of bFGF, TGF-Β1,BMSCs,and TCH gel; group B, treated with the combination of BMSCs and TCH gel;group C, treated with the combination of bFGF,TGF-Β1, and TCH gel;and group D, treated with PBS buffer solution. After the corresponding reagents were injected into the degenerative intervertebral discs of each group, the rats were cultivated for another four weeks and then the repair effects of the intervertebral discs were observed under MRI. Furthermore,the intervertebral discs of each group were taken out and observed by HE and Masson staining. The nucleus pulposus was aspirated and the expressions of aggrecan,collagen 2,Sox-9,and collagen I of nucleus pulposus of each group were tested by reverse transcription polymerase chain reaction and Western blot., Results: The transplanted BMSCs survived in the intervertebral disc and differentiated into nucleus pulposus-like cells. MRI showed that:the signal intensity of the nucleus pulposus of group A was much higher than that of the rest groups, the signal intensity of group B was higher than that of group C, and the signal intensity of group D was the lowest,in which the dura mater spinalis was in compression and the spinal cord changed in beaded shape. The differences of the Pfirrmann grading among the four groups had statistical significance (P<0.05). The results of the HE and Masson stains showed:the intervertebral disc of group A was well-structured,the quantity of nucleus pulposus cells was larger than that of the other three groups,and the boundary between the nucleus pulposus and the annulus fibrosus was clearly defined;the quantity of the nucleus pulposus cells of group B was larger than that of group C, and the broken annulus fibrosus was not observed in group B, while the broken annulus fibrosus could be observed in group C; and, the nucleus pulposus cells of group D were replaced by fibrous tissue. The results of the reverse transcription polymerase chain reaction and Western blot tests showed that,in terms of the expressions of aggrecan,collagen 2 and Sox-9,group A was the highest, followed by group B,group C,and group D (P<0.05); in terms of the expression of collagen 1,there was no obvious difference among these four groups (P>0.05)., Conclusions: The transplanted BMSCs can survive in the degenerative intervertebral disc and differentiate into nucleus pulposus-like cells. The combination of bFGF, TGF-Β1, BMSCs,and TCH gel has obvious repair effect on the degenerative intervertebral discs. The effect of the combination of BMSCs and TCH gel on transplantation therapy of the degenerative intervertebral discs is better than that of the combination of bFGF, TGF-Β1 and TCH gel but worse than that of the combination of bFGF, TGF-Β1, BMSCs, and TCH gel.

Objective: To observe the toxity of niclosamide on the growth of silkworms., Methods: Mulberry leaves were soaked in the different concentrations of solution of 50% ethanol salt powder of niclosamide for 10 s or 3 min, and then the leaves were fed to silkworms. The death rates of silkworms and the weight of cocoons were observed. Normal feeding control groups were established., Results: The death rates were 22.5%-85.0% and 56.0%-100% in the 10-s-soaked groups and 3-min groups, respectively, which were significantly higher than those in the control groups., Conclusion: The 50% ethanol salt powder of niclosamide has a toxity for silkworm. Therefore, we should properly handle the relationship between the Oncomelania snail control and silkworm breeding.

There is a growing attention on the environmental pollution and loss of potential regeneration of resources due to the poor handling of organic wastes, while earthworm vermicomposting and larvae bioconversion are well-known as two promising biotechnologies for sustainable wastes treatments, where earthworms or housefly larvae are employed to convert the organic wastes into humus like material, together with value-added worm product. Taken earthworm ( Eisenia foetida) and housefly larvae ( Musca domestica) as model species, this work illustrates fundamental definition and principle, operational process, technical mechanism, main factors, and bio-chemical features of organisms of these two technologies. Integrated with the physical and biochemical mechanisms, processes of biomass conversion, intestinal digestion, enzyme degradation and microflora decomposition are comprehensively reviewed on waste treatments with purposes of waste reduction, value-addition, and stabilization.

By using soil and water assessment tool (SWAT) model, this paper simulated the surface runoff intensity and the export loadings of sediment particulates and nutrients via non-point source hydrological pathway in Tiaoxi watershed, and integrated with the simulation results, analyzed the temporal and spatial distribution characteristics of non-point source pollution in the watershed in 2008. In the study area, the per unit area non-point source pollution was stronger in northern region than in southern region and in eastern region than in western region, and the weakest in central region. Among the land utilization types, farmland had the biggest contribution to the sediment loading. There were significantly positive correlations between the loadings of surface runoff and associated sediment particulates and the rainfall intensity. The export loadings of nutrients through surface runoff were higher in rainy season (from June to September) than in dry season (from December to next March), and there existed significant correlations between the surface runoff loadings of sediment particulates, organic nitrogen, and nitrate and the average gradient of lands.

Objective: To observe the expression levels of heat shock protein 70 (hsp70) and NF-kappaB p65 mRNA in lung tissue of acute paraquat (PQ) poisoning rats, and intervention effects of ulinastatin (UTI)., Methods: Seventy-two Sprague-Dawley (SD) rats were randomly divided into three groups: PQ poisoning group, UTI group and control group. The rats were exposed intragastrically to PQ at the dose of 80 mg/kg to establish a model of the rat acute lung injury. The UTI group was intervened by peritoneal injection with 10000 U/kg UTI in 30 minutes. On the 12, 24, 48, 72 h after exposure, myeloperoxidase (MPO) activity in lung tissue were detected. The expression of the NF-kappaB p65 mRNA and hsp70 mRNA in lung tissue was detected by the reverse transcription-PCR (RT-PCR). The lung pathological changes of rats were observed., Results: The degree of lung injury in PQ group and UTI group was higher than that in control group. But in UTI group the degree of lung injury was lower than PQ group. MPO activity in the lung tissues in PQ group was (31.72 +/- 6.42), (56.23 +/- 8.63), (87.21 +/- 10.02) and (107.21 +/- 13.52) micro/g in 12, 24, 48 and 72 h, respectively which was significantly higher than that [(11.38 +/- 1.25) micro/g] in control group (P < 0.01). MPO activity in the lung tissues in UTI group was (15.65 +/- 3.21), (35.98 +/- 5.74), (59.33 +/- 9.65) and (71.25 +/- 10.58) micro/g in 12, 24, 48 and 72 h, respectively which was significantly lower than those in PQ group (P < 0.01). The expression levels of NF-kappaB p65 mRNA of lung tissues in UTI group in 12, 24, 48 and 72 h were 0.3288 +/- 0.0147, 0.5337 +/- 0.0328, 0.7357 +/- 0.0424 and 0.7547 +/- 0.0905, respectively, which were significantly lower that those (0.4185 +/- 0.0294, 0.8532 +/- 0.0841, 0.9554 +/- 0.0975 and 1.0094 +/- 0.0703) in PQ group (P < 0.01). hsp70 mRNA expression levels in 12, 24, 48 and 72 h of the UTI group were 0.5193 +/- 0.0254, 0.8289 +/- 0.0606, 0.7566 +/- 0.0277 and 0.4873 +/- 0.0105, respectively, which were significantly higher than those (0.3897 +/- 0.0125, 0.5904 +/- 0.0186, 0.4007 +/- 0.0237 and 0.2293 +/- 0.0137) in PQ group (P < 0.01)., Conclusion: The expression levels of hsp70 mRNA and NF-kappaB p65 mRNA of rats after intoxication increased significantly. UTI can protect the lung tissues by elevating the expression of hsp70 and reducing the expression of NF-kappaB in the lung tissues of rats with acute paraquat poisoning.

Discovery of histone lysine specific demethylase 1 (LSD1) indicates that even histone methylation is reversible. Structural analysis shows that LSD1 is a flavin-dependent amine oxidase, which is able to catalyze the specific removal of methyl groups from mono- and dimethylated Lys4 and Lys9 of histone H3. Functional studies demonstrate that LSD1 regulates activation and inhibition of gene transcription in the nucleus, which is known as the innermost gene switch of cells. LSD1 plays important roles in embryonic development and tumorigenesis. Here, we review recent insights into the structure and chemical mechanism of LSD1, and its regulatory roles in development and cancer.

Healthy wetland system is regarded as an effective way for biological remediation of non-point source pollutants. A case field investigation on phosphorus (P) status of overlying water and sediment was carried out for Xiazhuhu wetland located in Northern Zhejiang Province, China. A static wetland microcosm experiment was conducted to understand the characteristics and mechanisms related to P exchanging, P forms changing, and water quality impact across the interface of water and sediment. Field investigation showed that total P (TP) concentrations of sediments were found from 0.187 mg x g(-1) to 0.591 mg x g(-1), and TP in overlying water reached from 0.022 mg x L(-1) to 0.718 mg x L(-1) where the seasonal concentration variations of TP, dissolved P (DP), and particulate P (PP) were commonly found in order as winter > summer > spring > fall. Fed by synthetic solution containing P levels of 0.0 - 10.5 mg x L(-1), a 35-day-lasting microcosm study showed that P retention by sediments could be divided into three basic phases in order, i.e., buffer reaction, rapid adsorption, and slow adsorption. Under a typical stress concentration of 1.0 mg x L(-1) in overlying water, the increment of P tanks in different forms was found as NH4 Cl-P (0.0%), Fe-P/Mn-P (around 20%), NaOH-TP (around 66%, mainly as the form of Al-P), Ca-P (1.9%), and Res-P (11.3%), on the condition of Xiazhuhu wetland under low water season. Application of Al to wetland would increase the capacity of sediment P retention in Xiazhuhu wetland.

The wastewater from animal farms in China is threatening the quality and security of local water environment. In the laboratory scale, a traditional SBR (A/O) and a denitrifying phosphorus-accumulating organisms (DNPAO) sequencing batch reactor (SBR) in biologically removing nutrients and organic matters from swine manure were investigated in this study. When piggery wastewater was directly treated with a traditional SBR, the efficiency was low with unstable working state, the reduction of TN, TP and BOD5 reached 89.08%, 86.04% and 93.88%, respectively. While it performs a good effect in treating high density of piggery wastewater using DNPAO SBR technology, with the help of low-density aeration, two-step of influent feeding and two circulations of anaerobic-anoxic-anaerobic-anoxic (An/Ax2), the reduction of TN, TP and BOD5 reached the maximums of 93.95%, 99.26% and 99.93%, respectively. The specific operation of (An/Ax2) SBR contributed both low concentrations and limited export loads of NO3-. The temporal curves of ORP, pH and DO over time could be indicators for simultaneous removals of nitrogen and phosphorus along with biodegradation of organic matter during this specific SBR running. A pilot-scale investigation was recommended to further testify the feasibility and efficiency of this kind of SBR under the real-world condition.

Loss of life expectancy (LLE) was used to evaluate and quantitate the health risk caused by carcinogen and non-carcinogen pollutants, and was proposed as a new method to compare the environmental health risk of different pollutants. The environmental health risks exposed by 6 major environmental pollutants in China, i.e., arsenic, DDTs, BaP, lead, cadmium and mercury, were evaluated and compared by using LLE. The LLE caused by carcinogen pollutants such as arsenic, DDTs and BaP are 3.6 d, 2.2 d and 12.1 d respectively, while the corresponding values caused by non-carcinogen pollutants such as lead,mercury and cadmium are 1.1 d, 1.7 d and 5.8 d respectively. Based on the studies published before, the rank of the health risk of the selected six environmental pollutants is BaP > cadmium > arsenic > DDTs > mercury > lead. The results reveal that the LLE can be utilized as a new index to evaluate and quantitate the health risk, and the environmental health risk caused by carcinogen and non-carcinogen pollutants can be compared within an unitive criterion by LLE.

Using a multi-layer soil column device, the effect of new nitrification inhibitor DMPP (3,4-dimethyl pyrazole phosphate) on nitrogen leaching was studied for understanding the nitrogen vertical transformation and lowering the nitrogen leaching losses. The results indicate that, within 60 days of experiment, the regular urea added with 1% of DMPP can effectively inhibit the ammonium oxidation in the soil, and improve the concentration of NH4(+) -N in soil solution over the 20 cm tilth profile, while decline the concentrations of NO3(-) -N and N2(-) -N. No obvious difference is found on NH4(+) -N concentrations collected from deep layer soil solution under 20 cm between regular urea and the urea added with 1% of DMPP. There is also no significant difference for the NH4(+) -N and NO3(-) -N in the soil solution of deep layer under 40 cm among the treatments of urea by adding with 1% of DMPP within 60 days. So DMPP could be used as an effective nitrification inhibitor to control ammonium oxidation, decline the nitrate leaching losses, minimize the underground water pollution risk and be beneficial for the ecological environment.

Recently, mesenchymal stem cells (MSCs) have been one of the target cells of gene engineering. To construct the lentiviral (LV) vectors carrying the brain-derived neurotrophic factor (Bdnf) gene, the rat mesenchymal stem cells (rMSCs) were infected and finally the Bdnf gene-modified rMSCs was obtained. The CDS region of the rat Bdnf gene was obtained with reverse transcriptase-polymerase chain reaction (RT-PCR), and the transfer plasmid (PNL-BDNF-IRES2-EGFP) of the LV vector was constructed. The three plasmids of LV vector: PNL-BDNF-IRES2-EGFP, HELPER, and VSVG were cotransfected to 293T cells to produce the LV vectors, which enabled the coexpression of the Bdnf gene and the enhanced green fluorescent protein (Egfp) gene. rMSCs were separated from the bone marrow of 2-month-old F344 rats, cultured in vitro, and identified. rMSCs were infected by the LV vectors that were produced already and were identified with fluorescent microscope, RT-PCR, immunocytochemical staining, and western blot. The result of sequencing showed that the sequence of the cloned Bdnf gene was consistent with that reported in the GenBank. The PNL-BDNF-IRES2-EGFP plasmid that was identified showed the correct sequence. After the 3 plasmids of LV vectors were cotransfected to the 293T cells, considerable green fluorescence in 293T cells was observed under the fluorescent microscope; the supernatant was collected and concentrated using ultracentrifugation, and the titer of the replication-defective LV vector particles measured was found to be 6.7 x 10(7) TU/mL. After the constructed LV vectors infected the rMSCs, the results obtained using RT-PCR, immunocytochemical staining, and western blot showed that the expression of BDNF in the Bdnf-rMSCs group (experimental group, EG) was significantly higher than that in the PNL-IRES2-EGFP-rMSCs group (mock group, MG) and the rMSCs group (control group, CG) at both mRNA and protein levels. LV vectors carrying the Bdnf gene were constructed successfully. The Bdnf gene-modified rMSCs could express BDNF to a higher degree. This greatly facilitates the next step in the study, such as the long period of therapeutic observation of cerebral ischemia with Bdnf gene-modified rMSCs.

Objective: To observe the efficacy of biological dressing containing calcium and magnesia (sheep dermis absorbing calcium and magnesia and cross-link with glutaraldehyde) on the management of hydrofluoric acid burns in rats and patients., Methods: Wistar rats were randomly divided into A ( n = 24, normal control, with isotonic saline dressing after burns), B ( n = 32, with isotonic saline dressing treatment after hydrofluoric acid burns), C ( n = 32, with wet-dressing treatment after hydrofluoric acid burns), and D ( n = 32, with biological dressing treatment after hydrofluoric acid burns) groups. The rats in the latter 3 groups were inflicted with 3 cm x 3 cm TBSA full-thickness burns, and mortality, concentration of blood calcium , histopathological observation were carried out at 4,8,24 and 72 postburn hours (PBH), with 8 rats at each time-points. In addition, 46 patients with hydrofluoric acid burns were divided into E (with wet-dressing treatment) and F (with biological dressing treatment) groups to compare the curative effect., Results: The mortality in A,B,C,D groups were 0,31.2% ,15. 6% ,6. 2% , respectively. The wound in B group was deepened gradually after burns, but that in D group was slighter when compared with that in C group. The concentration of blood calcium in A group was higher than that in B, C and D groups at each time-points, and that in D groups was higher than that in B and C groups. The concentration of blood calcium in D group at 8 and 24 PBH were [(2.215 +/-0.008) ,(2.216 +/-0.008) mmol/L], which were obviously higher than those in B [(1.813 +/-0.017),(1.912 +/-0.013)mmol/L l] and C [(2.015 +/-0.006), (2.018 +/-0. 010)mmol/L] groups, (P <0. 01). The clinical outcome in E group was much better than that in F group., Conclusion: Biological dressing containing calcium and magnesium can be applied in the emergency management and following treatment after hydrofluoric acid burns.

Objective: To investigate the incidence and clinical and endoscopic characteristics of Barrett's esophagus (BE) and the relationship between BE and reflux esophagitis (RE)., Methods: Patients with BE were diagnosed according to new standard by endoscopy and histology. The prevalence, form in endoscopy, histology of biopsy, age, sex, symptom, Helicobacter pylori (Hp) infection and relationship between BE and RE were analyzed., Results: BE was 7.90% of the total endoscopy number, and male vs female was 1.98, mean age (45.1 +/- 14.8) years old. Only 19.15% of BE patients had typical reflux symptoms. 81.20% of BE patients were with short segment BE. Intestinal metaplasia was detected in 34.83% of BE and dysplasia 9.88%. 39.94% of BE patients were infected with Hp. Patients with RE (2.07%) were significantly less than those of BE (P < 0.01). 8.42% of BE patients were also with RE and 32.11% of RE patients were also with BE., Conclusions: The incidence of BE in Fujian of China is high. Most of BE are short segment BE and male and have no typical reflux symptoms. Intestinal metaplasia is found in 1/3 of BE patients. There isn't a close association between BE and RE.

Objective: To study the effect of Dachuanxiongwan on the expression of vascular epithelial growth factor in the rats after focal cerebral ischemia and reperfusion., Methods: The ischemia and reperfusion model was established by blocking the middle cerebral artery of the rats with a nylon thread for two hours. Twenty male Sprague-Dawley rats with middle cerebral artery occlusion (MCAO) were randomly divided into two groups. One of the group of rats were administered with DCXW, 4.13 g/kg x d, twice a day for 3 days by gavaging, while other group were given the same volume of saline as a control. Seventy-two hours after reperfusion, the neuropathological function of the rats were evaluated according to Longa. The rats were then sacrificed and the samples of cerebrum were processed and embedded in paraffin and cut into sections. The expression of VEGF was determined by immunocytochemistry., Results: The score of the neuropathological function of the DCXW treated group (1.5 +/- 0.71) was lower than that of the control group (2.3 +/- 0.82, P = 0.032). More VEGF-positive cells were found in the rats treated with DCXW than those in the control group (P = 0.000)., Conclusion: DCXW upgrades the expression of VEGF and may have a positive effect on the cerebrum following ischemia and reperfusion.

Objective: To investigate the value of B-ultrasonography, CT and MRI in the evaluation of efficacy of radiofrequency ablation (RFA) for hepatic cancer., Methods: One hundred patients with hepatic cancer were treated by ultrasound-guided RFA between October 1999 and September 2000. All patients had been examined by serum AFP, B-ultrasound, CT or MRI before and within one month after RFA., Results: Before RFA, 34 patients who had had CT, the tumor showed hypo- or iso-density un-enhancement and enhancement on dynamic scanning. After RFA, 14 patients were examined by CT scan. Compared with the density on CT scan before RFA, 5 patients showed lower density lesion without any enhancement on dynamic scanning, but the other 9 patients showed similar images to the previous CT scan before RFA in some parts of their tumor. Before RFA, 66 patients examined by MRI showed hypo-intensity on T(1)-weighted image, hyper-intensity on T(2)-weighted image and enhancement on dynamic scanning. After RFA, among 86 patients examined by MRI, 44 showed iso- or hyper-intensity on T(1)-weighted image, iso- or hypo-intensity on T(2)-weighted image and no enhancement on dynamic scanning. But 42 patients showed similar images to the previous MRI imaging performed before RFA in some parts of their tumor., Conclusion: Both CT and MRI can be used as imaging evaluation tool on the effect of radiofrequency ablation for hepatic cancer. However, MRI is better than CT to detect whether the tumor is necrotic or still partly viable after radiofrequency ablation. Patients can be regarded as clinically cured provided that the serum AFP declines to the normal level from abnormally high level and/or MRI or CT scans show a complete necrotic lesion after RFA.

Objective: Studying the protective effect of dachuanxiongwan (DCXW) on the rats with focal cerebral ischemia and reperfusion., Methods: The ischemia and reperfusion model was established by blocking the rat's middle cerebral artery with a pre-set nylon thread and removing it from the internal carotid artery 2 hours after occlusion. 20 healthy male Sprague-Dawley rats following middle cerebral artery occlusion (MCAO) were randomly divided into two groups: Group A, transient ischemia, for control; Group B, transient ischemia treated with DCXW via gavage, 4.13 g/kg x d, twice a day for 3 days, while group A being given the same volume of saline. Seventy-two hours after reperfusion, the neurological function of all rats was evaluated according to Longa, and the rats were sacrificed. All the samples of cerebrum were processed and embedded in paraffin and cut into sections. The sections were stained with Hematoxylin & Eosin to measure the infarction volume and evaluate the effects of DCXW., Results: The grade of neurological function of the treatment group (1.5+/-0.71) was lower than that of the control group (2.3+/-0.82), there was a statistically significant difference between the two groups (P=0.032); the infarction volume of the treatment group was smaller than that of the control group, there was a statistically significant difference between the two groups (P=0.000)., Conclusion: The findings suggest that DCXW has a protective effect on the cerebrum undergoing ischemia and reperfusion.

Objective: To explore the feasibility, effect and problems of percutaneous radiofrequency ablation (PRFA) performed for small liver cancer located in hepatis., Methods: Twenty-one patients, who had small primary or metastatic liver cancer confirmed clinically or pathologically that were located in hepatis and less than 5 cm, were performed PRFA between April 2000 and October 2002. All patients were followed up to examine the value of AFP, MRI or CT. Kaplan-Meier estimation was used for the disease-free survival rate and the long-term survival rate., Results: The rate of AFP positive down to negative was 77.8% (7/9). The complete necrosis rate was 90.5% (19/21). The peri-tumor recurrence-free survival rates of 0.5-, 1-, 1.5-, 2-year were all 94.7%. The distant recurrence-free survival rates of 0.5-, 1-, 1.5-, 2-year were 90.0%, 77.1%, 77.1% and 77.1% respectively. The whole survival rates of 0.5-, 1-, 1.5- and 2-year were 89.2%, 82.8%, 82.8% and 55.2% respectively., Conclusions: Small liver cancer located in hepatis was not the contra-indication of PRFA. If the puncture point and route is selected properly, electrodes outspreaded exactly and the range of heating controlled appropriately, PRFA is an effective method and of less complication rate for small liver cancer located in hepatis. Sometimes, PRFA can be combined with TACE for those tumors of the diameter larger than 3 cm.

Objective: To observe the curative effect of percutaneous radio frequency ablation (PRFA) on hepatocellular carcinoma., Methods: Under local or epidural anaesthesia, radio frequency electrodes were percutaneously introduced with ultrasound guide into the tumor in the liver for ablation., Results: Among the 96 hepatic cancer lesions in 60 patients, 41 (42.7%) with diameter < 3.5 cm were ablated by one session. The effective rate (CR + PR) was 100% (41/41) with CR 92.6% (38/41) and PR 7.3% (3/41). During the 6 - 24 month follow up of these lesions, 36 (87.8%) of these 41 patients showed no recurrence by CT or MRI. Fifty-five (57.3%) lesions with diameter 3.5 cm < or = Phi < 12 cm were ablated by two or three sessions, with CR 3.6% (2/55), PR 67.3% (37/55) and CR + PR 70.9% (39/55) by CT or MRI in 1 to 3 months after the treatment., Conclusion: Percutaneous radio frequency ablation is effective on liver tumor with Phi < 3.5 cm. It is partly effective on lesions with 3.5 cm < or = Phi < 12 cm.