1. [Study of rapid method on enterotoxigenic Escherichia coli detected by real-time fluoresence quantitative PCR].
- Author
-
Dai J, Li Y, Yang X, and Yuan L
- Subjects
- Enterotoxigenic Escherichia coli genetics, Fluorescent Dyes, Oligonucleotide Probes chemistry, Oligonucleotide Probes genetics, Sensitivity and Specificity, Enterotoxigenic Escherichia coli isolation & purification, Real-Time Polymerase Chain Reaction methods
- Abstract
Objective: To develop a real-time PCR for detecting enterotoxigenic Escherichia coli (ETEC) based on TaqMan technology., Methods: Primers and probes were designed in the coding region of heat-stable enterotoxin, heat-labile enterotoxin of ETEC. ETEC were detected by real-time fluoresence quantitative PCR, making use of the exterior standard curve which was described by several different concentration. The speciality, sensitivity, accuracy, repetition, and stability of real-time fluoresence quantitative PCR system were evaluated., Results: Primers and TaqMan probes were suited to the real-time fluoresence quantitative PCR. The assay showed that the method could be rapid, special, sensitive and stabile. The real-time PCR system could detect ETEC between 10(0)-10(7) DNA copies/reaction. The assay should be finished in two hours., Conclusion: It was suggested that real-time fluoresence quantitative PCR based on TaqMan probe could be a rapid, sensitive and special method. It is significant that the excellent method could control diarrhoea caused by ETEC.
- Published
- 2008