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Objective: To develop a real-time PCR for detecting enterotoxigenic Escherichia coli (ETEC) based on TaqMan technology., Methods: Primers and probes were designed in the coding region of heat-stable enterotoxin, heat-labile enterotoxin of ETEC. ETEC were detected by real-time fluoresence quantitative PCR, making use of the exterior standard curve which was described by several different concentration. The speciality, sensitivity, accuracy, repetition, and stability of real-time fluoresence quantitative PCR system were evaluated., Results: Primers and TaqMan probes were suited to the real-time fluoresence quantitative PCR. The assay showed that the method could be rapid, special, sensitive and stabile. The real-time PCR system could detect ETEC between 10(0)-10(7) DNA copies/reaction. The assay should be finished in two hours., Conclusion: It was suggested that real-time fluoresence quantitative PCR based on TaqMan probe could be a rapid, sensitive and special method. It is significant that the excellent method could control diarrhoea caused by ETEC.

Objective: By investigating the changes of filamin distribution in renal tubular epithelial cells at different ischemia/reperfusion time stages we studied the role of filamin in kidney ischemia/reperfusion injury., Methods: The neonatal SD rat ischemia/reperfusion model was set up. Immunofluorescent staining was used to show the distribution of filamin in renal tubular epithelial cells and the quantities of filamin were counted by ImagePlus-Pro system., Results: Filamin was located around the basal plasma membrane in normal renal tubular epithelial cells. After ischemia for 0.5 h, the redistribution was not significant. After reperfusion for 0.5 h, filamin came into the cell plasma, appeared at the apex of cells and in the lumen. This kind of change was most apparent after reperfusion for 2 h and accompanied with destruction of renal tubules. The regeneration started 24 h post-ischemia. Filamin was redistributed to the basal plasma membrane. After reperfusion for 120 h, the regeneration ended and the structure of renal tubules became normal., Conclusion: The distribution of filamin was changed during ischemia/reperfusion injury, this change was prior to the change of actin cytoskeleton and integrin.