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[ Objective ] In vitro culture technique of reproducing buds from buds of Dendiobium officinale was established to provide technical support for selecting good varieties and propagating germchit of Dendrobium officinale in Guangxi. [Method] The effects of different sterilize time and different ratio of hormones on the inducing culture process of multiple shoots were researched by inducing, multiplying, strengthening and seeding the explants which were stems of wild Dendrobium officinale offspring materials form Guiping, Rongxian and Xilin. [Result] The best sterilize time of three materials was 10 min, and the contamination rate was 10.0%-20.0%. The best inducing medium of multiple shoots of Guiping's material was MS+2.0 mg/L 6-BA+0.2 mg/L NAA + 10% coconut milk, in which inductivity was 7.8%. The best inducing medium of multiple shoots of Rongxian and Xilin materials was MS + 1.5 mg/L 6-BA+0.2 mg/L NAA+10% coconut milk, in which inductivity was 23.1% and 30.8%. The best successive transfer culture medium of all these 3 materials was MS+6-BA 0.5 mg/L+NAA 0.2 mg/L+10% coconut milk, in which average proliteration ratio was 3-5 times. The best rooting medium was ½MS+0.5 mg/L NAA+0.2 mg/L IBA + 10% banana puree, in which rooting percentage was more then 95.0%. The medium of transplanting was pine bark, in which surviving rate of transplant reached 90.0%. [Conclusion] All of these three stem explants from wild Dendrobium officinale offspring materials form Guiping, Rongxian, Xilin can be used to build an in Vitro culture system of reproducing buds from buds. It can increase the inductivity and proliferation rate when increasing the transfer times on inducing medium and successive transfer culture medium. [ABSTRACT FROM AUTHOR]

A method for designing the submillimeter wave sub-harmonically pumped mixer based on a hybrid circuit model is presented in this article. To resolve the difficulty of extracting the Schottky diode pair's model for submillimeter wave band mixer, a full wave electromagnetic analysis was implemented to extract the passive circuit's parameters, and the DC I-V curve was used to characterize the diode cells' nonlinear performance. The hybrid circuit model was established. On the basis of the hybrid model, a 2 mm band subharmonical pumped mixer was designed and realized. The measured results show that its frequency conversion loss is less than 20 dB from 116 GHz to 120 GHz. The measured conversion loss agrees well with the simulated one, which shows the validity of the proposed method as well as the hybrid circuit model. [ABSTRACT FROM AUTHOR]

Objective: To summarize the experiences of binding pancreaticogastrostomy in pancreatoduodenectomy., Methods: Retrospective analysis was performed for the data of 36 patients undergoing pancreatoduodenectomy from January 2010 to January 2013. Binding pancreaticogastrostomy was used to reconstruct digestive tract. The postoperative complications were observed. And follow-ups were conducted on the patency of anastomotic stoma of pancreatogastrostomy and pancreatic duct., Results: All operations were successful. There were simple pancreatic fistula (n = 7, 19.4%), upper gastrointestinal tract bleeding (n = 2, 5.6%), delayed gastric emptying (n = 1), intra-abdominal infection (n = 1) and infection of incision (n = 3). All were cured with conservative therapy. There was no instance of bile leakage, intra-abdominal bleeding, reoperation or in-hospital mortality., Conclusions: Binding pancreaticogastrostomy causes no serious pancreatic fistula and complications. And it is a safe and reliable reconstructive approach with manageable follow-ups.

The dynamic adsorption of trichloroethylene (TCE), 1,2-dichloroethane (DCE) and trichloromethane (TCM) vapors onto hydrophobic hypercrosslinked polymeric resin (LC-1) were investigated using the fixed-bed adsorption method. The results indicated that the breakthrough time decreased and the height of mass transfer zone increased with the elevated initial concentration, gas flow rate and adsorption temperature. The gas flow rate had the wost significant influence on breakthrough time and height of mass transfer zone among the three factors. In addition, a simple semi-empirical mathematic model developed by Yoon and Nelson was applied to investigate the breakthrough behavior, and all correlation coefficients R2 were greater than 0.994.

Objective: To summarize the clinical features of the duodenal lipomas in order to explore effective diagnostic methods and appropriate treatment preoperatively., Methods: The clinical features, laboratory results, endoscopic appearance, radiological and pathological data of 8 cases of duodenal lipoma were retrospectively analyzed., Results: Four patients suffered with repeated melena, 3 cases with epigastric discomfort, sour regurgitation or hiccup, while 1 patient without any symptoms. Liver functions, serum lipids and tumor markers were normal in all patients. Six patients had been detected lesions by gastroscopy (2 cases missed diagnosed in the first examination), these lesions were appeared in duodenal bulb (2 cases) or descendant duodenum (4 cases). Abdominal CT examination revealed partial duodenal wall thickening (6 cases), partial enteric cavity narrowing (4 cases), or low-density lesions in enteric cavity (3 cases) with CT value of -85 HU and evenly intensified when enhanced. EUS showed intensive hyperechoic lesions from submucosa, with homogeneous echo and clear margin. Biopsy under endoscopy in all patients showed chronic inflammation of mucosa, while the pathologic diagnosis was lipoma after surgical excision or endoscopic resection. Pancreaticoduodenectomy performed in 1 patient, duodenal tumorectomy in 3 patients and endoscopic resection with snare in 4 patients., Conclusion: Common site of duodenal lipoma is descending part, and the clinical manifestations are non-specific. Imaging and endoscopic examination are the mainly methods to detect the lesion, while EUS is significantly valuable in diagnosing and differential diagnosing. It can be treated by partial tumorectomy or endoscopic trap resection.

Objective: To investigate the impact of experimental brain injury on the noradrenergic (NA) neurons in the spinal cord, in order to explore possible mechanism of the changes in NA neuron expression., Methods: Seventy healthy Wistar rats were randomly divided into two groups: normal group (n=10), brain injury (BI) group (n=60). The animals in BI group were subjected to BI with the modified Feeney's method. The spinal cords of rats were harvested at 3, 6, 12, 24, 48 and 72 hours after the injury, respectively. Immunohistochemistry (IHC) and reverse transcriptase-polymerase chain reaction (RT-PCR) were used to assess the changes in dopamine-beta-hydroxylase (DBH) expression., Results: IHC showed a small number of DBH-positive neurons distributed in anterior horn of the cervical and lumbar enlargements in control group; a larger number of dark-stained noradrenergic neurons were found in anterior horn, and also in posterior and lateral horns of spinal cord in brain injury group at different time points after injury, as compared with control group (P<0.05 or P<0.01). RT-PCR showed that the expression of DBH mRNA in brain injury group was significantly higher than that in control group at different time points after injury (P<0.05 or P<0.01)., Conclusion: A significant increase of NA neurons in spinal cord of brain injury group suggests that more NA is synthesized by the neurons, which is necessary to meet the demand of the organism under the stress condition, suggesting that NA plays a very important role in the course of brain injury.

Objective: To investigate the apoptosis and caspase-12 expression in progressive compression of spinal cord (PCSC)., Methods: 120 adult Wistar rats were randomly divided into 2 equal groups, experimental group, undergoing operation so as to establish PCSC models, and control group, undergoing sham operation. 1, 3, 7, 14, 21, and 28 days after the operation, 5 rats from each group were anesthetized with their spinal cords taken out. TUNNEL method was used to observe the apoptosis of the neurons in the compressed segments. Another 5 rats from each group at different time points were anesthetized with their spinal cords as well. Immunohistochemistry was used to detect the mRNA expression of caspase-12 in the compressed segments. Western blotting was used to detect the protein expression of caspase-12., Results: The apoptotic rates of neurons and gliocytes 1, 3, 7, 14, 21, and 28 days after were 12.5% +/- 2.3%, 13.0% +/- 3.6%, 17.2% +/- 4.3%, 29.4% +/- 4.4%, 36.1% +/- 6.5%, and 2.3% +/- 7.9% respectively, with significant differences among the values 14, 21, and 28 days and those at other time points after the operation (all P < 0.05). Immunohistochemistry showed that the number of caspase-12 positive neurons increased since 1 day after, and became remarkably high 14, 21, and 28 days after with significant differences among different time points (all P < 0.05). Western blotting showed that the protein expression of caspase-12 was low 1, 3, and 7 days after, and peaked 14 days after, and then gradually decreased, however, the expression levels 21 and 28 days after were still significantly higher then those 1, 3, and 7 days after (all P < 0.05)., Conclusion: Caspase-12 is involved in the apoptosis of neurons in PCSC.

RAPD (Random Amolified Polymorphism DNA) was employed to detect molecular markers linked to sex in S. grosvenorii by BSA (Bulked Segregant Analysis). 18 RAPD markers linked to sex were selected in BSA by screening 90 primers. Only the marker amplified by S1431 was present in all 8 male individuals tested while absent from all 8 female individuals tested. It showed that S1431 was a male RAPD marker linked to sex.

To investigate effects of rat bone marrow mesenchymal stem cells (rBMMSC) on hematopoiesis after allo-hematopoietic stem cell transplantation (HSCT), allogeneic BMT model from Fischer 344 rats (RT-1Al) to Wistar rats (RT-1Au) was established; effects of MSCs on hematopoietic reconstitution were studied by survival rate, peripheral blood counts, histological analysis and FACS at day 30 after transplantation. The results showed that (1) MSCs from donor Fisher344 could survive in recipient irradiated by lethal dose and could be found in the thymus, spleen and bone marrow of the recipient at 30 days after cotransplantation with BM by measuring EGFP gene. (2) Cotransplanation of MSCs and BM improved hematopoietic reconstitution. Lymphocyte and platelet counts of peripheral blood in cotransplantation group were higher than those in the control group. Active hematopoiesis and increase of bone marrow nucleated cells were observed in cotransplantation group. MSCs significantly enhanced hematopoiesis of B lymphocyte and megakaryocytopoietic lineages by FACS analysis. It is concluded that (1) MSCs of Fisher344 can be found in the thymus, spleen, bone marrow of the recipients at 30 days after cotransplantion by measuring EGFP gene. (2) hematopoietic reconstitution is significantly enhanced by MSCs cotransplanted with BM.