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Objective: To investigate the physicochemical properties and functional changes of the polysaccharides prepared from Sargassum fusiforme by different combinations of enzymes. Methods: Six enzyme combinations of cellulase-alcalase (C-A), pectinase-alcalase (P-A), viscozyme-alcalase (V-A), cellulase-neutrase (C-N), pectinase-neutrase (P-N), and viscozyme-neutrase (V-N) were used to assist the extraction of polysaccharides from Sargassum fusiforme. The physicochemical properties, structural composition, and antioxidant activity in vitro and in vivo of Sargassum fusiforme polysaccharides prepared by six combined enzymes were analyzed. Results: The contents of total sugar, uronic acid and sulfate in the polysaccharides prepared by the six combined enzymes changed greatly, and the monosaccharide composition and chemical structure were similar. The total sugar content and polysaccharide yield of the C-N group were the highest. The reducing power of Fe2+ and DPPH radical scavenging capacity of polysaccharides were the highest in the V-N group, amounting to 0.103±0.003 (Abs) and 0.540±0.022 (mg/mL) (IC50), respectively, and in the P-N group, the hydroxyl radical scavenging capacity of polysaccharides was the highest at 1.341±0.265 (mg/mL) (IC50). The polysaccharides prepared by different combined enzymes had different protective effects on AAPH-induced oxidative stress. Among them, compared with the NO group, the polysaccharides in the P-A, C-A, V-A and P-N groups had a stronger ability to reduce reactive oxygen species. The polysaccharides in the P-A, P-N and V-N groups had significant lipid peroxidation inhibition. Compared with the induction group, the polysaccharides in the C-N and V-N groups significantly increased CAT enzyme activity (P

Background and objective Targeted therapies are ineffective in lung squamous cancer (LUSC), and the low response rate of immunotherapy hampers its application in LUSC, so it is urgent to explore new strategies for LUSC treatment. Ferroptosis plays an important role in tumour suppression. The aim of this study was to investigate the role and mechanism of targeting 3-hydroxy-3-methylglutaryl-CoA synthase 1 (HMGCS1) in regulating ferroptosis in LUSC cells, in order to provide a new research direction for LUSC therapy. Methods The expression of HMGCS1 in LUSC was analysed by The Cancer Genome Atlas (TCGA) and Clinical Proteomic Tumor Analysis Consortium (CPTAC) online databases; the relationship between HMGCS1 and survival time of lung cancer was analysed by the Kaplan-Meier Plotter online survival database; the expression level of HMGCS1 in LUSC tissues was verified by immunohistochemistry. After interfering with HMGCS1 expression by small interfering RNA (siRNA), cell activity and cell migration ability were detected by CCK8 and Transwell assay; apoptosis was detected by flow cytometry after interfering with HMGCS1 or after treatment with the HMGCS1 inhibitor of hymeglusin; Fe2+, reactive oxygen species (ROS) and lipid peroxidation levels were detected by flow cytometry and high-content confocal fluorescence imaging systems, respectively in SKMES cells after inhibition of HMGCS1; and Western blot was performed to detect the expression of ACSL4, GPX4 and SLC7A11, which are markers of the ferroptosis pathway after inhibition of HMGCS1. Results HMGCS1 mRNA and protein levels were significantly high in LUSC; siRNA interference with HMGCS1 expression inhibited the proliferative activity and migration ability of LUSC cells, but had no significant effect on apoptosis. Interference with HMGCS1 or treatment with the HMGCS1 inhibitor of hymeglusin significantly promoted intracellular Fe2+, ROS and lipid peroxidation levels in SKMES cells, and induced ferroptosis in LUSC cells; Western blot assay showed that inhibition of HMGCS1 significantly promoted the expression of ACSL4. Conclusion Inhibition of HMGCS1, a target of LUSC, promotes ferroptosis in lung cancer cells and provides a research basis for screening new therapeutic targets for LUSC.

In order to achieve the goal of autonomous networks and improve the automation of network operation, an autonomous network model was explored.The model introduced algorithms such as multidimensional data automatic clustering, adaptive sliding time window mechanism, user traffic loss assessment model based on spatiotemporal sequence correlation, fault propagation graph based on graph theory and spatiotemporal clustering, and builded automation capabilities through network event management, fault self-identification, self-positioning, self-scheduling, and intelligent quality inspection.The goal is achieving L4 advanced intelligent network by 2025.

Objective: To study the curing process of health meal replacement powder (HMR) and evaluate its anti-depression function. Methods: The mixed traditional Chinese medicine powder of HMR was cured by stewing, stir-frying, microwave and other processes. Meanwhile, the physicochemical indexes, total polysaccharides, total flavonoids and characteristics in vitro digestive were determined. The depression model mice were established by intraperitoneal injection of LPS, and mice were randomly divided into blank control group, model control group, fluoxetine hydrochloride group and HMR group. Using the tail suspension test and forced swimming test in mice, the antidepressant effect of HMR was observed. The behavior, IL-1β, TNF-α, 5-HT, NE and BDNF of mice were determined. Results: After curing, the water solubility index (WSI), water absorption index (WAI), centrifugal sediment rate (SR), dispersibility time, wettability time, total polysaccharide and total flavonoids content of extrusion group were 34.35%, 3.18 g/g, 52.16%, 184.12 s, 195.02 s, 32.2 mg/g and 0.62 mg/g. The results showed that extrusion could not improve the soakage and disperal of Chinese medicine powder as much as other curing processes like baking, but it could significantly increase the content of soluble small molecules and active substances of Chinese medicine powder. Results of in vitro digestion showed that extrusion had a little effect on the release of total flavonoids from Chinese medicine powder, but the content of rapidly digestible starch (RDS) (63.52%) was lower, and the content of slowly digestible starch (SDS) (34.14%) was significantly higher than that of other groups (P

In order to improve the quality of early indica rice flour products by using endogenous proteins to regulate its physicochemical properties, this paper studied the effects of removing endogenous proteins with different solubility (albumin, globulin, prolamin and oryzenin) on the solubility, swelling capacity, and pasting properties of the early indica rice flour, as well as on its water binding state, rheological properties and freeze-thaw stability after pasting. The results showed that the removal of globulin and oryzenin could improve the solubility of the early indica rice flour, while the removal of prolamin and oryzenin could improve its swelling capacity under high temperature. The removal of albumin made the peak viscosity, final viscosity, breakdown value and setback value of the early indica rice flour decrease by 16%, 21%, 50% and 43%, respectively. The removal of globulin could increased its gelatinization temperature and decreased its peak viscosity, while the removal of prolamin was the opposite. The removal of oryzenin made its peak viscosity, and final viscosity decrease by 22% and 12%, respectively, as well as made its breakdown value and setback value increase by 49% and 53%, respectively. The removal of oryzenin made the weak bound water content of the early indica rice flour decrease by 14% after pasting. The removal of both globulin and oryzenin could increase its water binding strength after pasting, while the removal of prolamin was the opposite. The removal of prolamin made the solid-like state of the early indica rice flour enhance by 20% after pasting. The removal of both globulin and oryzenin could decrease its elasticity after pasting, while the removal of prolamin was the opposite. The removal of globulin and oryzenin made the freeze-thaw stability of the early indica rice flour decrease by 121% and 56%, respectively, after pasting. This study would provide an important reference for improving the quality of early indica rice flour products by using endogenous proteins with different solubility.

Epidermal growth factor receptor-tyrosine kinase inhibitors (EGFR-TKIs) targeting EGFR are effective in EGFR mutation-positive non-small cell lung cancer (NSCLC) patients, but drug resistance is inevitable. With the application and expansion of individualized and combined therapy, more and more studies have shown that combined administration of Metformin effectively solves the problem of acquired drug resistance to EGFR-TKIs in clinical treatment and prolongs the survival of patients with NSCLC. EGFR-TKIs combined with Metformin is expected to be the treatment method of choice for NSCLC patients with EGFR-TKIs resistance. This paper intends to summarize the research progress of EGFR-TKIs combined with Metformin in the treatment of EGFR-TKIs acquired resistance in NSCLC, in order to provide a new idea for the treatment of NSCLC patients with acquired resistance to EGFR-TKIs.

To explore the synergisic effect of DH (Dendrobium huoshanense) and KBT (Keemun black tea) on preventing immunity reduction. The solid-liquid ratio, extraction time and extraction times of DH and KBT were studied by single factor test and orthogonal test with the extraction rate of Dendrobium polysaccharide and black tea polyphenols as indexes, the extraction techniques of DH and Keemun black tea were optimized respectively. The optimal ratio of KBT and DH was determined by toxicity test and phagocytosis test on RAW264.7 cells. The results showed that the optimum extraction conditions of DH polysaccharide were as follows: The ratio of material to liquid was 1:80 (g/mL), the extraction time was 80 min, and the extraction times were 4 times. The optimum extraction conditions of KBT polyphenols were as follows: The ratio of material to liquid was 1:80 (g/mL), the extraction time was 25 min, and the extraction times were 5 times. The ratio of KBT to DH was determined by observing the effect of different proportions of the mixture on macrophages. The results of the in vitro experiment showed that the extract mixture of 75% KBT and 25% DH had the best immune-enhancing effect. The results of the in vivo test showed that the high-dose group of KTDH could significantly reduce the changes of levels of cytokines IL-2 and IL-6 due to immunosuppression in mice (P

On the basis of using electronic tongue and electronic nose to evaluate the quality of Zhaguangjiao, MiSeq high-throughput sequencing technology was used to analyze the bacterial diversity and explore the effect of different salt concentration on its quality. The results showed that with the increase of salt concentration, the taste and flavor indexes changed regularly. At the gate level, Firmicutes (84.87%) gradually increased, while Proteobacteria (13.65%) decreased gradually. At the genus level, the relative content of Lactobacillus increased gradually, and the average relative content was as high as 60.20%. When the salt concentration was 5%, the flora structure of Zhaguangjiao tended to be stable, and strength of good indicators (umami) in flavor and taste was moderate, while the relative intensity of defective indicators (sourness) was generally low. Thus, it can be seen that adding 5% salt to the Zhaguangjiao can significantly improve the quality of the Zhaguangjiao.

Based on the comprehensive analysis of core, thin section, logging and seismic data, this study carried out the identification and comparison of Permian Changxing Formation sequences, clarified the typical sedimentary architectures of intra-platform shoal, investigated the vertical and horizontal development and distribution of intra-platform shoal in each sequence, and thus established the sedimentary evolution model of shoal body. The study results are reflected in four aspects. First, there are two complete third-order sequences (SQ1 and SQ2) in Changxing Formation in central Sichuan Basin. SQ1 is generally thick in the north and thin in the south, and SQ2 shows a thickness differentiation trend of “two thicknesses and three thinnesses”. Second, the Changxing Formation in central Sichuan Basin mainly develops intra-platform shoal, inter-shoal sea and intra-platform depression subfacies. In the vertical direction, the intra-platform shoal mainly presents two typical sedimentary sequences: stable superposed and high-frequency interbedded. Third, the stable superimposed sedimentary sequence is developed in the shoal belt at the edge of intra-platform depression, which is composed of two shoal-forming periods and located in the highstand systems tracts (HSTs) of SQ1 and SQ2. The high-frequency interbedded sedimentary sequence is developed in the southern shoal belt of intra-platform depression, which is composed of four shoal-forming periods and mainly located in the HST of SQ2. Fourth, during the SQ1 deposition, the intra-platform shoal was mainly developed at the edge of the intra-platform depression on the north side of the study area, and the inter-shoal sea subfacies was mainly developed on the south side. During the SQ2 deposition, the intra-platform shoal was widely developed in the area, forming two nearly parallel intra-platform shoal belts. The study results provide direction and ideas for exploration of Changxing Formation intra-platform shoal reservoirs in central Sichuan Basin.

Objective To establish lymphoblastoid cell line (LCL) and human monoclonal antibody cell lines against PP1Pk antigens from a donor with rare p[Tj(a-)] phenotype. Methods Peripheral blood mononuclear cells (PBMCs)of an O type p[Tj(a-)] donor were prepared and transformed with Epstein-Barr virus (EBV) in vitro. Normal AB and O type red blood cells treated with bromelain as well as p[Tj (a-)] were used to screen the culture supernatant. The transformed LCL secreting antibodies to PP1Pk antigens was picked up and then hybridized with the SHM-D33 cell line using electric fusion technique. The hybridomas were performed by HOTD selective culture, antibody screening and limited dilution subcloning. Results We established a lymphoblastoid cell line 5E1-E5 and several monoclonal hybridoms cell lines secreting anti-PP1Pk immunoglobulin M from EBV transformed B lymphocytes of a p[Tj(a-)] type donor with anti-PP1Pk successfully. Conclusion Our work made it possible for screening rare p[Tj(a-)] blood type in donor population in China using human monoclonal anti-PP1Pk antibodies, which was important for the establishment of rare blood group banks in China.

With the deep integration of information technology and manufacturing, the networking of manufacturing transactions has become an inevitable trend.Cloud manufacturing services can realize cross-supplier transactions that are not limited by geographical space, but in the process of transaction, there are problems such as difficulty in guaranteeing the trust of both parties and leakage of privacy.In order to solve the above problems, a cloud manufacturing service platform architecture based on a dual-chain model was proposed, which stores user data and transaction data in separate chains, and adopts a practical Byzantine fault tolerance (PBFT) consensus algorithm to solve the problem of data synchronization between blocks.However, traditional PBFT consensus algorithm has bottlenecks in storage and consensus efficiency, and is not suitable for large-scale manufacturing platforms.Therefore, further research on the PBFT consensus algorithm was carried out.The election process was optimized and the consensus protocol process of the consensus cluster was improved by combining the EigenTrust model and the quality of service (QoS), and then give the manufacturing resource rent-seeking and matching steps.Analysis and simulation experiments show that this research effectively improves the reliability of PBFT consensus nodes, improves the operating efficiency of the platform and the speed of block consensus, and saves data storage space.

The traditional “centralized” access control technology can no longer guarantee the security of private data access control in the current internet of things environment.Based on the ABAC (attribute-based access control) model, a blockchain based access control framework for the internet of things and a private data access control model were proposed.Firstly, the basic framework and process of access control were described and analyzed in detail, and an auditable access control model was proposed to systematically manage the access control policies of private data through the requests, responses and access records stored in the blockchain network.Then, an auditable access control system based on blockchain technology was proposed, which can provide distributed, fine-grained and dynamic access control management in the internet of things, realize the effective management and auditable access to data, and adopt the access control method based on smart contract to realize the transparent, traceable and automatic access control over the internet of things resources.Finally, simulation experiments and performance tests verify the effectiveness and security of the access control model and system.

[Objective] The differences in the dominant driving factors of NDVI evolution in different sub-regions and different historical periods in China were determined in order to provide important decision support for the protection and restoration of vegetation ecosystems. [Methods] Based on GIMMS NDVI, MODIS NDVI, and meteorological station data, we used a center of gravity model and geodetector to analyze and determine temporal and spatial evolution patterns and the driving mechanism of NDVI in China from the perspective of geographic division. [Results] ① In the past 40 years during 1981—2019, the center of gravity of NDVI in China has shifted southward, indicating that the increment and growth rate of vegetation NDVI in Southern China were greater than in Northern China. ② Regions with positive correlations between NDVI and temperature were mainly located in the Loess Plateau, the middle of the Sichuan Basin, the Yunnan-Guizhou Plateau, and the middle and lower reaches of the Yangtze River. Zones with negative correlations between NDVI and precipitation were mainly located in the middle and lower reaches of the Yangtze River and the Yunnan-Guizhou Plateau; ③ During 1981—2019, there were significant differences in the dominant driving factors of vegetation evolution in different regions and different historical periods. With the increasing intensity of human activities over time, land use and other factors have gradually become the dominant factors in the evolution process of vegetation ecosystems in each sub-region. [Conclusion] The vegetation condition in China has generally increased in the past 40 years, and the contribution rate attributed to human activities in the evolution of vegetation ecosystems has become greater than that of natural factors.

To explore the effects of three pepper varieties on bacterial taxa and quality of Zhaguangjiao, Zhaguangjiao prepared using Capsicum frutescens Linn., Capsicum annuum L., and Capsicum annuum L. var. grossum (L.) Sendt. for the test group, while two control groups without the addition of any vegetables and with the addition of only Apium graveolens var. rapaceum were set up, and based on that, the quality of Zhaguangjiao was digitally evaluated and its bacterial taxa were resolved by high-throughput sequencing. Finally, the correlations between Zhaguangjiao quality and dominant bacterial genera were revealed. It was found that, compared with Zhaguangjiao prepared using Capsicum frutescens Linn., both the bacterial diversity and abundance of Zhaguangjiao prepared using Capsicum annuum L. and Capsicum annuum L. var. grossum (L.) Sendt. were higher. Zhaguangjiao prepared using Capsicum annuum L. and Capsicum annuum L. var. grossum (L.) Sendt. were dominated by Lactobacillus with average relative abundances of 77.53% and 94.41%, respectively, while Zhaguangjiao prepared using Capsicum frutescens Linn. was dominated by Pseudomonas with an average relative abundance of 67.35%. Compared with Zhaguangjiao prepared using Capsicum frutescens Linn., the sourness and content of aromatic compounds of Zhaguangjiao prepared using Capsicum frutescens Linn. was dominated by Pseudomonas were significantly higher, while the contents of organic sulfides, terpenes and nitrogen oxides among volatile substances were significantly lower. After Pearson correlation analysis, it was found that elevating the abundance of Lactobacillus in the fermentation system of Zhaguangjiao could obviously suppress the undesirable flavor and improve the content of aroma substances in Zhaguangjiao. Zhaguangjiao is popular for its sour and spicy taste, due to Capsicum annuum L. var. grossum (L.) Sendt. is less spicy, choosing Capsicum annuum L. as the main raw material to produce Zhaguangjiao has a positive effect enhancing the overall quality of the product.

Mining key nodes in the network plays a great role in the evolution of information dissemination, virus marketing, and public opinion control, etc.The identification of key nodes can effectively help to control network attacks, detect financial risks, suppress the spread of viruses diseases and rumors, and prevent terrorist attacks.In order to break through the limitations of existing node influence assessment methods with high algorithmic complexity and low accuracy, as well as one-sided perspective of assessing the intrinsic action mechanism of evaluation metrics, a comprehensive influence (CI) assessment algorithm for identifying critical nodes was proposed, which simultaneously processes the local and global topology of the network to perform node importance.The global attributes in the algorithm consider the information entropy of neighboring nodes and the shortest distance nodes between nodes to represent the local attributes of nodes, and the weight ratio of global and local attributes was adjusted by a parameter.By using the SIR (susceptible infected recovered) model and Kendall correlation coefficient as evaluation criteria, experimental analysis on real-world networks of different scales shows that the proposed method is superior to some well-known heuristic algorithms such as betweenness centrality (BC), closeness centrality (CC), gravity index centrality(GIC), and global structure model (GSM), and has better ranking monotonicity, more stable metric results, more adaptable to network topologies, and is applicable to most of the real networks with different structure of real networks.

Objective To analyze the influence of β-lactam antibiotics on RBC aging and clearance by detecting various indicators of aging and clearance on RBCs, as well as the differences in phagocytosis for erythrocytes before and after drugs treated in vitro. Methods RBCs were treated by β-lactam antibiotics, including Penicillin, Cefepime, Cefoperazone and Ceftazidime, and the changing of phosphatidylserine (PS) and clearance related CD markers, including CD35, CD47, CD55 and CD59 on the surface of the RBCs, were detected by flow cytometry at 0h and 24h after drugs treatment. The proportion of acanthocytes by microscope also at 0h and 24h after drugs treatment was calculated. The phagocytosis of drug-treated RBC was detected by monocyte monolayer assay (MMA). Untreated RBCs were incubated in PBS by the same condition as a negative control.The influence of β-lactam antibiotics on RBC aging and clearance by all the results above was studied. Results Compare to the untreated RBCs, the drug treated RBCs showed a higher PS level on the cell surface. The results showed by percentage as following(0 h vs 24 h): Penicillin 9.42% vs 93.30%, Cefepime 3.88% vs 57.27%, Cefoperazone 4.71% vs 75.75% and Ceftazidime 3.05% vs 43.19%. The acanthocytes ratio was as following(0 h vs 24 h): Penicillin 7.33% vs 86%, Cefepime 2.67% vs 52.67%, Cefoperazone 3.33% vs 67.67% and Ceftazidime 3.33% vs 90.67%. On the opposite, the clearance related CD markers, showed an obviously lower level after drugs treated(0 h vs 24 h): CD35: Penicillin 7.36% vs 11.87%, Cefepime 0.14% vs 28.51%, Cefoperazone 11.85% vs 21.55% and Ceftazidime 7.63% vs 8.73%; CD47: Penicillin 1.22% vs 9.13%, Cefepime 1.80% vs 0.86%, Cefoperazone 0.08% vs 6.85% and Ceftazidime 1.54% vs 5.50%; CD55: Penicillin 14.46% vs 44.31%, Cefepime 17.27% vs 38.41%, Cefoperazone 19.28% vs 33.28% and Ceftazidime 14.62% vs 34.13%; CD59: Penicillin 4.71% vs 20.56%, Cefepime 4.03% vs 7.60%, Cefoperazone 5.91% vs 22.38% and Ceftazidime 5.93% vs 30.89%. Drug-treated RBCs attached more to monocytes than untreated RBCs. Conclusion The β-lactam antibiotics could induce the changing of PS and the clearance of related CD markers on surface of RBCs. They also could lead acanthocytes and make the RBCs more susceptible to phagocytosis by monocytes. The β-lactam antibiotics could promote the RBCs aging and clearance, which might deteriorate the DIIHA.

Post-fire debris flow is a special torrent containing a large amount of loose material, which is closely related to forest fire. Its characteristics are significantly different from the conventional debris flow. Therefore, conventional debris flow prevention experience is not completely applicable to it. After the fire that broke out on March 30 in Xichang, debris flow occurred frequently in burned area, seriously threatening the safety of local people's lives and property. It is necessary and urgent to study the characteristics of debris flow after fire and put forward comprehensive prevention and control measures. This study takes Dianchichang gully in Xichang, Liangshan Yi Autonomous Prefecture, Sichuan Province as a typical case. Analyzed the characteristics of burned area and watershed. Studied the characteristics of post-fire debris flow disaster. And proposed the corresponding prevention and control measures. The results show that the burned area covered 83.37%, seriously burned areas accounted for 33.47%, resulting in widely spread ash layer. Slope provenance accounted for 69.83%. Post-fire debris flow occurred one month after the forest fire, and a total of 5 debris flows occurred in the first rainy season. Moreover, the threshold of debris flow starting rainfall is small and the occurrence of debris flow increases rapidly. The post-fire debris flows in the study site are characterized by high frequency and sudden occurrence. Accordingly, the corresponding prevention and control measures were propound as follows. The rapid emergency treatment measures include clarifying dead wood on slope surface, vegetation restoration, and building of simple barrier dams in gully. Medium-term governance measures are debris dams in gully and grit basins. And long-term prevention measures are a combination of forest fire prevention and vegetation protection. This scheme has been implemented in Dianchichang gully. Since the completion of these projects, the effects of comprehensive treatments have been preliminarily proved effective for the mitigation of this type of special debris flow. It can provide reference for post-fire debris flow prevention in the future.

In recent years, paste technology has been rapidly promoted in domestic mines. It is a key technology for realizing green mining because it can effectively solve environmental and safety problems caused by mining. With the progress in science and technology, the research level of paste technology is constantly improving. Thickening technology of tailings is the primary procedure of paste backfill technology in metal mines, which can significantly improve the efficiency of tailings dewatering and utilization rate of tailings. Tailings thickening technology is an important part of the green development of mines. This paper presented a summary of the development process of tailings thickening and dewatering technology, dividing the development stage of the thickener into three: (1) ordinary thickener stage, (2) high-efficiency thickener stage, and (3) paste thickener stage. The application status of the thickening process of tailings and several typical application cases at home and abroad were also described. In addition, this paper explored different theories, including the single flocculation theory, multiple flocculation theory, bed compression theory under static/dynamic compression, and gravity thickening theory, along with the corresponding latest research progress of each theory. This paper also presented the main research methods of thickening of tailings, namely the static settlement experiment, small-scale thickening experiment, and semi-industrial thickening experiment. Advanced observational methods such as focused beam reflection measurement technology (FBRM) and particle video microscope technology (PVM) were also introduced. Moreover, the paper expounded on the research status of numerical simulation of tailings thickening technology and highlighted that the tailings thickening and dewatering technology is still in the development stage with some underlying problems, such as the instability of the key parameters of tailings thickening, the untimely production control of tailings thickening, and the imperfect information platform of tailings thickening. Overall, the development of tailings thickening technology is still facing numerous challenges. Finally, the direction of the development of tailing thickening technology is proposed in terms of personalization, automation, and intelligence.

Objective: To investigate the optimal dosage of contrast agent in three-dimensional dynamic enhanced magnetic resonance angiography (3D CE-MRA) in the imaging of arterial lesions of both lower limbs. Methods: 45 patients who underwent 3D CE-MRA angiography of lower extremity arteries in our hospital were randomly divided into three groups A, B and C. The gadolinium dosages of the three groups were respectively 0.1 mmol/kg, 0.2 mmol/kg and 0.3 mmol/kg. They were injected intravenously with saline at the ratio of 1:1. The images of different dose scanning schemes were scored subjectively and objectively. Subjective scoring: the lower limb arteries were divided into femoral artery, popliteal artery and calf artery (posterior tibial artery, anterior tibial artery and common peroneal artery). The quality of MIP reconstruction images was evaluated, and the overall venous overlap scoring of calf and foot vessels was also carried out. Finally, the differences of the three scanning schemes were compared by Wilcoxon test.The objective scoring is to measure and calculate the signal intensity Si, signal-to-noise ratio SNR and contrast-to-noise ratio of the original enhanced images of the three groups, compare the horizontal signal intensity differences of femoral artery, popliteal artery and calf artery, and compare the differences of the three scanning schemes by t-test. Results: When the contrast medium dosage was 0.1 mmol/kg, the image quality scores of femoral artery, popliteal artery and calf artery were (3.35±0.25), (2.97±0.25), (2.35±1.15) respectively; When the dosage of contrast agent was 0.2 mmol/kg, the scores of the above parts were (3.75±0.35), (3.55±0.32), (2.97±0.70) respectively; When the dosage of contrast agent was 0.3 mmol/kg, the scores of the above parts were (3.90±0.41), (3.83±0.52), (3.10±0.75) respectively. 33.33% of the images in group A did not meet the diagnostic needs, while all the images in group B and C met the diagnostic needs. There was significant difference between group A and group B, and there was no significant difference between group B and group C. B The Si, SNR and CNR of femoral artery and popliteal artery in group C were significantly higher than those in group A, and there was no significant difference between group B and C. The images of group B and C were better than those of group A. Conclusion: Appropriate increase of the contrast medium dosage is helpful to improve the 3D CE-MRA imaging quality of both lower limb arteries. The contrast medium dosage of 0.2 mmol/kg is reliable and accurate for the evaluation of blood vessels, and can provide accurate and reliable imaging basis for the formulation of treatment plan for patients with peripheral artery diseases.

Objective To establish human hybridoma cell lines, secreting monoclonal antibody against antigens of Rh blood system, from a donor with rare D--phenotype. Methods Peripheral blood B lymphocytes of an O type female donor, lacking C/c/E/e antigens on her erythrocyte, were transformed with Epstein-Barr virus (EBVs). EBVs were harvested from the cultural supernatant of B95-8 cells. The transformed lymphoblastoid cell line (LCL) secreting antibodies to C antigens were picked up and then hybridized with the myeloma SHM-D33 using electric fusion technique. Hybridoma cells were selected by HATD-Ouabain(HOTD)(Hypoxantine, Aminopterin, Thymidine, 2-Deoxycytide, and Ouabain)culture medium, microplate antibody screening and limited dilution subcloning. The monoclonal antibody was assayed by serological test and was confirmed by flow cytometry (FCM). Results From the cultural supernatant of D--peripheral blood transformed B lymphocytes, 3A6-C6, which agglutinated with R1R1(DCe/DCe)O-type RBCs but not with R2R2(DcE/DcE)O-type RBCs, was screened and preliminarily identified as anti-C. We established a hybridoma cell line secreting anti-C immunoglobulin M from B cells of D--individual successfully after hybridization with SHM-D33 myeloma cells. Conclusion The study had laid the groundwork for future research and development of human monoclonal antibodies against Rh antigens of RBC in future for diagnosis and screening purpose.

Longde County in southern Ningxia is located at the western foot of Liupan mountain. Geological conditions is complicated. Affected by seasonal heavy rainfall, landslides and geological disasters occur frequently in the area, which poses a serious threat to local people's lives and property. In consideration of the high fractional vegetation in study area, integrated remote sensing technologies combined of Synthetic Aperture Radar Difference Interferometry technology and high spatial resolution optical remote sensing have been used in early detection of Landslides in the Longde county. Stacking technology has been used to calculate the rate of deformation from 2019.01 to 2021.05, in the direction of ascending and descending orbit. Combining high spatial resolution optical remote sensing images and digital elevation model (DEM), the interpretation key of landslide in the area deformation has been established. Then the early detection of landslides and ground survey in Longde county have been done. Through the integrated remote sensing technologies, 47 landslides were detected. 21 landslides were surveyed by field, of which 16 were verified, with an accuracy rate of 71.4%. The results of field survey demonstrated the applicability and feasibility of integrated remote sensing technology in the detection of landslides in southern Ningxia. Meanwhile, and the accuracy of the results in Longde county has been testified. The results of the early detection through integrated remote sensing technology provided significant scientific bases for the landslide protection and emergency response to sudden geological disasters in southern Ningxia.

This paper studied the characteristics of geomorphology, fire behavior, unconsolidated source material volume, runoff and sediment production related to post-fire debris flow formations, field investigation in the burned area and rainfall simulation experiment were adopted to study the post-fire debris flow in Huayanzi gully, Mianning County, Sichuan Province occurred on 4 July 2019. The associated ash layer, water repellency and permeability experiments were also conducted. The results suggest that (1) the runoff charge shows a positive correlation with water repellency in burned area. (2) the sediment yields in severely burned area are conspicuously larger than that in moderate and slight burned areas. (3) the water repellency in burned area is approximately positively correlated with fire severity, while the permeability presents the negative correlation. This study revealed the evolution process of post-fire debris flow, and provided theoretical basis for the prevention and early warning of post-fire debris flows.

As a country rich in oil and gas resources, Turkmenistan's natural gas industry has become a pillar industry of its national economy. However, Turkmenistan's natural gas export shows a weak growth trend due to insufficient productivity construction and blocked export channels. At present, Turkmenistan has natural gas export lines from Russia in north, Iran in south and China in east. Whereas China is the only major buyer of its natural gas due to the influence of geopolitical and economic interests. To get rid of such predicament, Turkmenistan actively promoted the diversification of natural gas export. On the one hand, it expanded new export channels, vigorously promoted the implementation of TAPI project to South Asia, and at the same time discussed the Trans-Caspian Natural Gas Pipeline Project with each stakeholder. On the other hand, Turkmenistan fully promoted the resource utilization of natural gas in the country, vigorously developed natural gas chemical industry and power generation, and converted existing natural gas resources into products with high added value for export. Turkmenistan, as a source country of China's natural gas imports, has an irreplaceable position in China's natural gas imports from Central Asia. It is the most realistic in the current relations between China and Turkmenistan to tamp down the performance of the China–Turkmenistan natural gas trade contract and to guard against Turkmenistan's reduction in gas supply. In the future, the possibility of establishing a large natural gas pipeline network from Central Asia to South Asia, covering China, Iran, Turkmenistan, Azerbaijan, Pakistan and India, may be explored based on TAPI and IPI projects.

The massive MIMO system is equipped with a large number of antennas at the base station to serve many users, but it is affected by the inter-cell interference which called pilot contamination. A pilot scheduling scheme was designed to reduce the effect of pilot contamination. Pilot scheduling problem belongs to the arrangement optimization problem, and the algorithm computational complexity of greedy algorithm to search the optimal solution is greatly. In order to reduce the influence of the interference of the community, a new pilot scheduling schemes was proposed by so this article using cell classification and cross entropy(CE) mechanism to reduce the effect of pilot contamination on the system. The simulation results show that the proposed algorithm can not only improve the performance of the system, but also reduce the computational complexity.

In order to better study the channel assignment problem, a function from the vertex set to the set of all nonnegative integers is generated, that is the L(2,1)—labeling of a graph. Let the least label be zero, the L(2,1)—labeling number of a graph is the smallest number over the spans of all L(2,1)—labeling of this graph. Aiming at the problem of the L(2,1)—labeling numbers of the bracelet graph, which is a generalized graph from Cartesian products of the path and cycles, the definition of the bracelet graph is given, which is obtained by overlapping the two ends of a similarity ladder. At the same time the definition of the L(2,1)—labeling numbers is given. The L(2,1)—labeling number is completely determined by vertex grouped labeling method according to the difference of the circles' numbers and the vertices' numbers of the circles. The types of graphs are enriched and the labeling number theories are perfected.

The mechanism of MC and its impact on the received signal vector were analyzed.A closed-form expression and an approximate expression of SC for antenna arrays with different forms were derived,where the power angle spectrum of arrival signal was modeled as Von Mises (VM) distribution.On this basis,the general expressions of SC with MC effect was derived.Simulation results show that the proposed analytical expressions are in good agreement with the numerical integration results.In addition,under the mutual coupling effect,the correlation of antenna array elements fluctuates around the correlation curve without mutual coupling,and the planar array has better resistance to mutual coupling.

The distributed parallel computing in the super-resolution image enhancement applications was introduced.Super resolution image enhancement refers to improving the image resolution of 1080P video to 4K by software without upgrading existing acquisition device and increasing the transmission bandwidth.Due to the fact that super resolution image enhancement technology needs enormous computing power,super resolution image enhancement system and performance comparison testing by distributed parallel computing was studied.The conclusion shows that the super-image enhancement processing ability can be enhanced hundreds of times with distributed parallel computing technology,so that the production capacity of 4K video can be improved in rea1-world application.

The paper introduced present status of electric control system of domestic mine-used overhead man-riding device, discussed its development direction, and described the latest requirements of the state of electric control system of overhead man-riding device, which provides reference for design and application of the system.

This article describes the application of transition theory to assist a family with an infant with congenital complex gastroschisis. The nursing period, from March 3, 2023 to May 9, 2023, encompassed care from hospitalization to discharge. The author employed transition theory as a guide and used physical assessments, observations, and interviews for data collection as well as behavioral processes records. The primary nursing problem was identified as "preparation for family operation process enhancement/child's congenital disease and complex care needs, and the family's response to the challenges of the disease and care adaptation." The three phases of nursing care were summarized as: (1) the family adjustment to uncertainty, (2) undertaking caregiving roles and responsibilities, and (3) role development and family reconnection. The author established specific goals for each phase and provided corresponding interventions for the family. In the first phase, the author guided the family in expressing their concerns, and offered personalized health education information as well as psychological support to help them understand the progression of their child's disease and alleviate related anxiety and confusion. In the second phase, the author offered sleep guidance and customized home care schedules to support coping skill development and role functioning. In the third phase, the family was encouraged to explore the meaning of life while accompanying their child's growth in order to achieve spiritual growth and deepen the reconnection within the family. Ultimately, the family strengthened their confidence and capabilities in caregiving and embraced optimism and expectations for the future, enabling them to adapt smoothly to life after their child's return home. When families are confronted with their child's diagnosis with a congenital disease, they often find themselves in a state of self-doubt and faced with continuous challenges. Nurses may employ transition theory throughout the nursing process to better understand and address the evolving needs of both children and their families during the transition phase. Furthermore, transition theory may be applied to help nurses better assess, plan, and care for their patients, which can enhance the capabilities of families and facilitate their successful navigation through the challenging transition journey. [ABSTRACT FROM AUTHOR]

In this study, five walnuts varieties were used as research objects, and the phenotypic data (size, weight and component) and oxidative characteristics were gathered, then the differences of walnuts phenotypic characteristics were investigated using principal component analysis (PCA) and hierarchical clustering method. Results showed that three principal components were extracted by PCA, and the contribution rate of cumulative variance reached to 93.078 9%. Based on the combination of phenotype (size, weight, composition) and oxidation characteristics, systematic cluster analysis was used to intuitively reflect the indices differences of different walnuts varieties through clustering heat maps, which could effectively distinguish different varieties of walnuts. [ABSTRACT FROM AUTHOR]

PURPOSE: To assess the efficacy of cartilage dissection and repositioning in primary nasal reconstruction on long-term nasal symmetry and shape in complete unilateral cleft lip patients who received nasal molding. METHODS: 69 complete unilateral cleft lip patients underwent nasoalveolar molding before primary cheiloplasty. The primary cheiloplasty was done by the same surgeon. 32 (32/69) patients received cartilage dissection and repositioning in primary nasal reconstruction at the same time. Photographs were taken and used to evaluate the results 4 to 5 years postoperatively. The data was analyzed with SPSS 10.0 software package. RESULTS: The results revealed that the nasal symmetry and shape was significantly improved 4 to 5 years postoperatively after primary cheiloplasty simultaneously with cartilage dissection and repositioning in primary nasal reconstruction in complete unilateral cleft lip patients. The score of the two groups were 70.66 ±10.89 and 64.14 ±10.63, respectively. There was significant difference between the two groups (P<0.05). CONCLUSION: Primary cheiloplasty performed simultaneously with cartilage dissection and repositioning in primary nasal reconstruction can help to promote nasal symmetry and better appearance on long-term at an early age. This study demonstrates that simultaneous primary rhinoplasty with cartilage dissection and repositioning in primary nasal reconstruction can be more effective for complete unilateral cleft lip patients and no interference with nasal growth was encountered. Supported by Medical Research Fund of Shenzhen Science and Technology Bureau (Grant No.200602102). [ABSTRACT FROM AUTHOR]

Objective To investigate the effect of dorsomorphin, a bone morphogenetic protein (BMP) small-molecule inhibitor, on the migration and invasion abilities of drug-resistant esophageal cancer EC109/PTX cells. Methods MTT assay was used to observe the effect of different concentrations of dorsomorphin on the proliferation of EC109/PTX cells and determine the concentration for subsequent experiments. Dorsomorphin at the concentration of 1 μmol/L was established as the experimental group, and a blank control group was set up. EC109/PTX cells were treated for 48 h. Flow cytometry was used to observe the effect of dorsomorphin on the apoptosis of EC109/PTX cells; scratch assay and Transwell assay were used to observe the migration and invasion abilities of EC109/PTX cells; Western blotting was used to measure the relative protein expression levels of Vimentin, E-cadherin, and N-cadherin in EC109/PTX cells. Results Dorsomorphin at the concentration of 1 μmol/L showed an inhibition rate of (9.89±1.12)% on the proliferation of EC109/PTX cells. At 48 h of culture, there was no significant difference in the apoptosis rate of EC109/PTX cells between the control group and the experimental group (P>0.05). Scratch assay and Transwell assay showed that compared with the control group, the experimental group had significant reductions in the migration rate and cell permeation rate of EC109/PTX cells (t=85.42,19.65,P

Taste is an important quality attribute of tea. In order to find out the taste quality of green teas made from the major varieties in Wanzhou district, Chongqing, the taste profile of the tea infusions was determined by sensory evaluation and 5 taste components including catechins, free amino acids, alkaloids, flavanols and their glycosides, and organic acids were analyzed by targeted detection methods. The results showed that there were obvious differences in the taste quality of green teas prepared from the 4 varieties of tea trees. Sichuan middle- and small-leafed tea group variety had a strong taste, and ‘Anji Baicha’ had an obvious fresh and brisk taste with mellow sweet aftertaste. ‘Fuding Dabaicha’ tasted strong with fresh and brisk taste and mellow sweet aftertaste. ‘Zhonghuang 1’ tasted fresh and brisk but less strong. The results of taste component analysis showed that the Sichuan middle- and small-leafed tea group variety contained high contents of catechins but low contents of free amino acids. ‘Anji Baicha’ had the highest content of free amino acids. The content of each flavor component was high in ‘Fuding Dabaicha’, and the contents of total flavor components and water extract of ‘Zhonghuang 1’ were low. The results of principal component analysis (PCA) showed that the 4 varieties could be well distinguished. Thirteen major taste contributors such as rutin and caffeine were identified. The differential analysis indicated that aspartic acid, glutamic acid and theanine were the major contributors to the fresh and brisk taste characteristics of ‘Anji Baicha’ and ‘Fuding Dabaicha’. Yangmeiin-3-O-galactoside and chrysin were the major taste contributors for ‘Zhonghuang 1’ that distinguished it from the other 3 varieties. There were also large differences in taste components within the Sichuan middle- and small-leafed tea group variety, with samples C4 and C5 being closer to each other. They both contained high contents of flavonoid glycosides, having a strong astringent taste. This study provides a reference for research to improve the flavor quality of green tea in Wanzhou district.

Mycoplasma pneumoniae pneumonia （MPP） is a common cause of community-acquired pneumonia in children aged 5 and older in China. Although most affected children have favorable clinical prognosis， some cases can escalate into severe MPP due to risk factors such as pathogen resistance or co-infections， etc. These severe cases significantly jeopardize the lives of children and may lead to diverse long-term sequelae. In response to this challenge， the Children’s Medical Center of the Third Affiliated Hospital of Sun Yat-sen University， integrating current guidelines， expert consensus， and its extensive clinical experience， has devised the “412” cluster treatment strategy. This comprehensive approach encompasses treatments for anti-infection， anti-inflammatory storm， anticoagulation and antioxidant stress. Additionally， it seamlessly integrates traditional Chinese medicine with western therapies， featuring bundled respiratory management and follow-up care. This strategy provides a valuable reference for the diagnosis and treatment of severe MPP in children.

本研究基於原住民族語文課程和臺灣社會脈絡具高度相關性，相較其他 領域的課程發展，複雜程度又更高，因此，選擇原住民族語文課綱作為研究對象，探討其形塑歷程。為達此研究目的，本研究採訪談及文件資料的蒐集與分析方法。透過相關資料的分析後發現，原住民族語文課綱形塑歷程並非完全按最初規劃的程序運作，受到相關事件或因素影響，使得形塑軌跡產生非預期的轉折。此外，分析該課綱形塑歷程也發現，出現一些具爭論性的課程議題，包括領域課綱的疆界與內涵、課綱發展的組織與運作機制、學生課程修習的設計，以及學校教育實務現場的配套等。最後，本研究透過課程政策理論與形塑實際的對話，對原住民族語文課程綱要形塑的重要議題再進行綜合性反思，並針對所遭遇的問題，提出未來發展原住民族語文課程綱要的建議。 Indigenous language curriculum guidelines are highly relevant to Taiwan’s social context and more complex compared to curriculum development in other fields. Therefore, this study explores the development process of these guidelines within the framework of Twelve-Year Basic Education Curriculum. To achieve this research purpose, the study employs interviews and document analysis. Findings reveal that the process of crafting the indigenous language curriculum guidelines deviated from initial plans due to various events and factors, resulting in unexpected developments. The analysis also uncovered several controversial curriculum issues, including the scope and content of the field guidelines, the organizational structure for guideline development, the design of student course options, and practical implementation in schools. Through a dialogue between curriculum policy theory and actual practice, this study reflects on key issues in developing the indigenous language curriculum guidelines. Based on the challenges identified, recommendations for future refinement of these guidelines are proposed.

[Objective] Exploring the driving factors and dynamic change characteristics of the coupling relationship between ecosystem service trade-offs and synergies is crucial for clarifying the mutual feedback mechanism of social-ecological systems in the context of ecological restoration. [Methods] In this study, we selected Yan’an City, a typical vegetation restoration area, as the study area, and quantified four ecosystem services including carbon sequestration, water yield, baseflow regulation, and soil conservation from 2000 to 2020. Then we used a comprehensive ecosystem services index (ESI) to characterize the coupling relationship between the four ecosystem services. Based on the Bayesian belief networks (BBNs) model, we developed a network structure, which captured the driving factors of ESI and identified the variation characteristics of ESI. [Results] The results show that: (1) The ESI of Yan’an City increased significantly from 2000 to 2020, with the high value areas of ESI expanded from the middle to the north and south of the city, and the low value areas of ESI concentrated around the town and the northern fringe area. The baseflow regulation steadily declined since 2000. (2) The combined contribution rates of precipitation, fractional vegetation cover (FVC), actual evapotranspiration, and temperature to the change of ESI were more than 70% in 2000 to 2020, in which precipitation and FVC were the dominant driving factors. (3) In the 20-year study period, the contribution rate of precipitation showed a fluctuating downward trend but the contribution rate of FVC kept rising. According to the scenarios of probabilistic inference of BBNs, increasing temperature and precipitation will aggravate the risk of loss for baseflow regulation, while the continuous vegetation restoration will also lead to the threshold effects for the optimization of ESI. [Conclusion] A long-term monitoring of vegetation restoration areas and the improvement of baseflow regulation should be the priorities for Yan’an City in order to maintain the effectiveness of vegetation restoration and to cope with climate change and human disturbances in the future.

With the continuous development of China's offshore oil and gas resources, the corrosion of pipeline welded joints has become one of the main factors leading to the failure and safety of marine operating pipelines. In order to provide more accurate theoretical basis and practical guidance for corrosion protection and material selection of marine pipelines, the electrochemical corrosion behaviors of the base material and weld zone of X80 pipeline steel welded joints were studied in different NaCl solution concentrations(1.5%, 3.5%, 5.5%, mass fraction) via dynamic potential polarization technology and electrochemical impedance spectroscopy testing method. Results showed that in the solutions with different NaCl concentrations, the polarization curves of the base material and weld zone exhibited obvious anodic dissolution characteristics without passivation phenomenon. With the increase of NaCl concentration, the open circuit potential and self-corrosion potential of the two regions shifted negatively, the polarization resistance first decreased and then increased, and the self-corrosion current density first increased and then decreased. Furthermore, the thermodynamic stability and corrosion resistance of the base material zone were always superior to those of the weld zone.

Background Hyperuricemia (HUA) caused by elevated serum uric acid (SUA) has been shown to be an independent risk factor for the development and progression of chronic kidney disease (CKD). However, there are few cohort studies on the correlation of SUA level with the development and progression of CKD in the elderly of China. Objective To explore the association of baseline SUA level and its changes with the risk of chronic kidney disease (CKD) and estimated glomerular filtration rate (eGFR) in the elderly in longevity areas of China. Methods Based on the Healthy Aging and Biomarkers Cohort Study (HABCS), a sub cohort of the Chinese Longitudinal Healthy Longevity Survey (CLHLS), the older adults who received physical examination and with biomedical indicators in 2012 and 2014 were selected as the study subjects from December 2021 to May 2022. The age, gender, blood pressure, blood lipids, blood glucose and other biomedical indicators were collected at baseline and follow-up period. Cox proportional hazards regression model was used to analyze the association of different SUA levels with the risk of CKD. Spearman rank correlation and generalized linear model analysis were used to analyze the association between baseline SUA level and baseline eGFR level and the linear correlation between changes in SUA level and eGFR changes in the elderly, respectively. Results A total of 981 subjects were included in the study, with the median age of 79 (70, 88) years, the prevalence of HUA of 6.8% (67/981), the cumulative follow-up of 2 029 person-years and the median follow-up of 2.05 years, including 179 new cases of CKD, the cumulative incidence of CKD during the follow-up was 18.2%〔95%CI (15.9%, 20.8%) 〕, and the incidence density was 88.22/1 000 person-years〔95%CI (76.24/1 000 person-years, 101.41/1 000 person-years) 〕. Cox proportional hazards regression analysis with SUA quartile grouping as the independent variable showed that compared with the lowest quartile group of baseline SUA level (Q1), the HR value for the risk of CKD in the highest quartile group of baseline SUA level (Q4) was 2.08〔95%CI (1.27, 3.41), P=0.004〕. Cox proportional hazards regression analysis with SUA level as the independent variable showed that, for every 10 μmol/L increase in baseline SUA level, the risk of CKD in the elderly increased by 4% (P