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Purposes The high-value utilization of coal gangue has attracted attention, and the activation and action of coal-bearing kaolin have great significance value for utilization of coal gangue. Methods The thermal activation parameters of kaolinite in coal gangue were optimized by orthogonal test, and the compressive strength and microstructure of paste consisting of cement and metakaolin formed under the optimal activation conditions were studied. Findings The experimental results show that the optimum thermal activation conditions of metakaolin are calcination temperature 800 ℃, holding time 2 h, and grinding fineness of 2.82 μm. Calcination temperature has the greatest influence on the activity of metakaolin, followed by grinding fineness and holding time. By using metakaolin instead of 20% (mass fraction) cement, the compressive strength of cement paste at all curing ages, especially in the early age, can be improved effectively. XRD and SEM test results show that the pozzolanic reaction of metakaolin consumes a lot of calcium hydroxide, and forms calcium silicate hydrate with lower calcium-silicon ratio and denses structure.

The aim of this study was to evaluate the combined effect of gaseous ozone (OG) and perforated modified atmosphere packaging (MAP) on the preservation of Chinese bayberry. In order to determine the optimal storage conditions, different modified atmospheres and different concentrations of OG were used separately or in combination to treat Chinese bayberry. Results showed that the optimum modified atmosphere for packaging of Chinese bayberry was 10% O2 + 10% CO2 + 80% N2, which could maintain dynamic equilibrium when a microporous film with two perforations of 100 μm in diameter per 243.39 cm2 was used, and the optimal OG concentration was 18.3 mg/m3. The single treatments with OG or perforated MAP inhibited the decay incidence and maintained high contents of total phenols, anthocyanins and flavonoids in Chinese bayberry. The combined treatment was more effective in preserving the quality of Chinese bayberry, slowing down the decline of fruit firmness, and reducing the losses of total soluble solids (TSS), titratable acid (TA) and ascorbic acid (ASA). In addition, the activities of peroxidase (POD) and superoxide dismutase (SOD) in the combined treatment group were 1.42 and 1.9 times higher than those in the untreated control, respectively, indicating reduced accumulation of H2O2 and O2-·, so that the mold free shelf life was extended up to 8 d.

Background As an important part of diabetes treatment, insulin can be administered either by multiple daily injections (MDI) or continuous subcutaneous insulin infusion (CSII) via an insulin pump, but there are still controversies around the effectiveness of insulin administered by both methods in type 2 diabetes mellitus (T2DM) among older adults. Objective To evaluate the effectiveness of CSII in T2DM in older adults using meta-analysis, and to test the validity of the analysis results using trial sequential analysis (TSA) . Methods Cochrane Library, PubMed, Embase, Medline, Scopus, Web of Science, CNKI, Wanfang Data, CQVIP and SinoMed were retrieved for randomised controlled trials (RCTs) from inception to December 2021 regarding the effectiveness of CSII via an external insulin pump compared with that of MDI of insulin for older adults with T2DM. The primary outcomes included fasting plasma glucose (FPG), 2-hour postprandial plasma glucose (2 hPG), HbA1c and incidence of hypoglycaemia. The secondary outcomes included the mean amplitude of glycemic excursions (MAGE), total daily dose of insulin, and the time to achieve glycemic target. Two researchers independently carried out literature enrolment, quality evaluation and data extraction. RevMan 5.3 was adopted for meta-analysis. TSA v0.9 (Copenhagen Trial Unit) was adopted for TSA. Results Sixteen RCTs were included in total. Meta-analysis demonstrated that CSII via an external insulin pump outperformed MDI of insulin in improving FPG〔MD=-0.82, 95%CI (-1.09, -0.54), P

In view of the possible threats faced by air cushion landing craft in the process of assault landing, the regional cover and the accompanying cover method of air cushion landing craft by armed helicopter are proposed.In the area cover, the calculation model of the area cover is established, and the area cover method of the gunship is given. In along with the cover and set up the enemy missile boats to the threat of air cushion landing craft Angle to determine model, based on this model, combined with the equipment of the armed helicopter performance, gives the armed helicopter to the air cushion landing craft cover before the distance optimization model, combined with the simulation calculation, and gives the armed helicopter configuration of air cushion landing craft and cover method, It provides a theoretical basis for the reasonable use of armed helicopter in the assault landing process of hovercraft.

A method for the determination of 16 polycyclic aromatic hydrocarbons in vegetable oil by gel permeation chromatography purification coupled with GC-MS/MS was developed. The results were shown below: (1) During GPC purification of soybean oil sample added with 16 PAHs, the distillates of 0~19 minutes were oil impurities. The distillates of 19~28 minutes and 28~36 minutes were collected in sections and then measured by GC-MS/MS. The results showed that the distillates from 19 to 28 minutes were PAHs of the first ten low molecular weight and the distillates from 28 to 36 minutes were PAHs of the last six high molecular weight. So, GPC was not strictly in the order of high molecular weight first peak and low molecular weight second peak. Therefore, in order to ensure the accuracy of the results, the method of segmenting collection and segmenting determination should be adopted when separating various compounds by GPC. (2) Compared with pretreatment method in GB/T 23213—2008, this method reduced the amount of vegetable oil sample weighed from 4.0 g to 1.0 g. The target analytes in samples were purified using GPC to eliminate most of the coextracts. The extraction process was simplified so that the possible content loss in the extraction process was avoided. The experiment time was shortened and the extraction reagent was saved. (3) The calibration curves of the 16 PAHs showed good linearity in the range of 10~200 ng/mL with the correlation coefficients(R2) greater than 0.99. The LOD ranged from 0.02 to 0.50μg/kg and LOQ ranged from 0.08 to 1.67 μg/kg for 16 PAHs. (4)At the spiked level of 20 μL, 40 μL and 80 μL the average recoveries were 85.30%~103.86%, 91.08%~106.71% and 88.01%~105.87% respectively, and relative standard deviations(RSDs) were 0.69%~2.93%, 0.64%~2.74% and 0.75%~2.81% respectively. The method met the requirements of 16 PAHs analysis.

Objective: This study aimed to improve the accuracy and feeding speed of the cap arranging unit. Methods: Using SolidWorks to build a virtual model and manufacture a real object, and the kinematic simulation of bottle cap and capping machine was carried out in Adams. Moreover, the results of different speeds were compared and analyzed. ANSYS Workbench was used to carry out engineering simulation of the device components and obtain their natural frequency and vibration mode, and the structural performance was optimized. Results: The cap arranging unit had the minimum damage to the caps at the optimum speed. The optimal speed of the designed new cap sorter mechanism was 15 r/min, and the average number of caps unscrambling was 402 pieces/min. Conclusion: The operation of the new cap sorter mechanism exceeds the scheduled workload stably, which meets the operation requirements of the cap sorter mechanism.

A method for the determination of 20 pesticides residues in wheat by QuEChERS coupled with GC-MS/MS was developed. The effect of sorbent for purification was investigated. The matrix effect was also studied. The results showed that: (1) Compared with pretreatment method in 7.1.2 of GB 23200. 113—2018, this method reduced the amount of anhydrous MgSO4 added from 1200 mg to 400 mg, the amount of PSA added from 400 mg to 100 mg, and the amount of C18 added from 400 mg to 200 mg. Also, the add 100 mg GCB that could effectively remove the pigment in the wheat extract by optimizing the purification adsorbent. (2) The calibration curves of the 20 pesticides showed good linearity in the range of 5–500 ng/mL with the correlation coefficients (R2) greater than 0.99. The LOD ranged from 0.01 to 0.16 μg/kg and LOQ ranged from 0.04 to 0.52 μg/kg for 20 pesticides. The matrix effects of the 20 pesticides varied greatly. (3) At the spiked level of 80.0 μg/kg, 200.0 μg/kg and 400.0 μg/kg the average recoveries were 75.24%-98.29%, 82.01%-105.71% and 94.91%-106.14% respectively, and relative standard deviations (RSDs) were 0.57%-3.13%, 0.67%-3.98% and 1.26%-3.08% respectively. The method met the requirements of pesticide residue analysis.

In order to suppress the corrosion of carbon steel in ammonium chloride solution during hydrocracking, the relevant corrosion inhibitor was studied. 5-cyclohexyl-1,3,5-triazinane-2-thione was synthesized using thiourea, formaldehyde and cyclohexanamine as raw materials, and its structure was characterized using FTIR and 1H NMR. The corrosion inhibition performance of the product for 10 carbon steel in 2% NH4Cl solution was studied by weight loss method and electrochemical method. Results showed that the corrosion inhibition performance of 5-cyclohexyl-1,3,5-triazine-2-thione on 10 steel in 2% NH4Cl solution increased with the increase of its addition, and decreased with the increase of temperature. Electrochemical results showed that the corrosion inhibitor behaved as a mixed inhibitory type inhibitor at low temperature or a cathodic inhibitory inhibitor at high temperature. The adsorption of 5-cyclohexyl-1,3,5-triazinane-2-thione on the surface of 10 steel in 2% NH4Cl solution obeyed the Langmuir adsorption isotherm, and the adsorption belonged to spontaneous and exothermic processes.

For solving the scaling problem on the metal surface, by using polyvinylidene fluoride (PVDF) and polyperfluoroethylene propylene (FEP) as raw materials, the PVDF/FEP/ZnO composite coatings were prepared by adding different content of nano ZnO into PVDF and FEP. The effects of nano ZnO content on the hydrophobicity and antiscaling performance of the composite coatings were studied. In addition, contact angle measurement, scanning electron microscopy (SEM) and X-ray powder diffraction (XRD) were employed to characterize composite coatings systematically. Results showed that when the amount of nano ZnO was 5%(mass fracrion), the surface free energy of PVDF/FEP/ZnO composite coating was 8.6 mJ/m2, and the water contact angle (WCA) was 114.8°. After scaling in supersaturated calcium carbonate solution for 336 h, the scaling amount of CaCO3 on the surface of PVDF/FEP/ZnO composite coating was 72% of that of the epoxy coating, and the mole fraction of aragonite and aragonite reached 91.4%. In general, the PVDF/FEP/ZnO composite coating had excellent antiscaling performance, presenting a good application prospect in the field of antiscaling.

The research status of the influences of fillers on the corrosive resistance of anti - corrosive coatings at home and abroad in recent years was briefly described. And the research progress of the effect of dispersion processes, types, shapes, and addition amounts of fillers on the performance of anti - corrosive coatings was summarized. Then, the effects of four kinds of filler modification technologies on the corrosive resistance of anti - corrosive coatings were emphatically described. Finally, in order to provide theoretical basis and technical support for the development of environmentally friendly anti - corrosive coatings and the formulation of anti - corrosive measures for oil and gas field pipelines, the mechanism of the fillers to improve the corrosion resistance of the coating was discussed.

Telomerase reverse transcriptase is a subunit of telomerase, which plays an important role in activating telomerase and regulating cell cycle. Human telomerase reverse transcriptase(hTERT) is mainly expressed in germ cells, embryonic stem cells and tumor cells, only a few expressed in normal cell. hTERT is mainly located in nucleus and also distributed in mitochondria. hTERT migration from nucleus into mitochondria is called the mitochondrial translocation of hTERT. Mitochondrial translocation of hTERT functions in the process of apoptosis, immortalization and drug resistance. Control of hTERT is a potential breakthrough in the treatment of diseases.

In order to improve the feeding efficiency of the push-rod device of the pre-filled syringe, the simulation analysis was carried out based on the JKR model of EDEM software. With the push-rod as the research object and the discharge number in specified time period as the evaluation index, the influences of the inner tray rotation speed, the outer tray rotation speed, the tilt angle of the inner rotation tray, the angle of the cone tray and the outer rotation radius on the discharge efficiency of the push-rod were investigated. Firstly, single factor analysis was carried out to determine the influence of each single factor and screen out the four factors that with greater influence. Then the orthogonal design of four factors and three levels was carried out to determine the optimal parameter combination and the order of the influence degree of each factor on the discharge efficiency. The discharge stability was studied and the best feeding method was obtained. Finally, the experimental validation was carried out. The results show that the maximum error of simulation and test is 9.6%, which indicates that the simulation analysis of the model is reliable. Under the condition of the best parameter combination, the discharge efficiency is improved by 29.8% compared with the original data. [ABSTRACT FROM AUTHOR]

A new diagnosis method based on wavelet packet and neural network is proposed for the fault diagnosis of submarine cables.By this method,the fiber Brillouin frequency shifts in the cables are converted into temperature and strain data.The monitoring signals are first denoised by using the six-layer wavelet decomposition and the faulty data are decomposed and reconstructed by three-layer wavelet packet,and then such structural eigenvectors as energy,standard deviation and Shannon entropy are extracted and inputted to the Back Propagation（BP）neural network for training and testing.The experimental analysis shows that inputting the standard deviation as the eigenvector to the BP neural network is the optimum method for the fault diagnosis in submarine cables.

Two improved contributions have been advanced for the standard Sequential Monte Carlo Probability Hypothesis Density (SMC-PHD) filter. Firstly, a novel method is advanced for the cardinality and state estimation. A weight matrix is firstly calculated by measurements and persistent particles, and the weight sum of each row is then evaluated, the measurements indexed by row will be judged as true if its weight sum is larger than a certain threshold, and the weight sum of persistent particle states will be reported as the true target states. Secondly, an assistant variable which is used to denote the persistent age for every particle is introduced, by the help of this age variable, the overrated problem of targets number in dense clutter environment can be effectively restricted. The results of numerical simulation prove that the improved SMC-PHD filter has higher tracking performance than the standard one.

In order to solve problems that current gas-generation pretreatment system used in coal industry cannot fully meet requirements of mine safety and its automation degree is not high, the paper put forward a design scheme of gas-generation pretreatment system which accords with coal mine safety regulation, and expounded structure, realizing methods of each function part and software design scheme of the system. The system has some innovative designs about structure and control method, namely adding a fire-prevention and explosion-proof unit, adding a separator of gas and water and automatic drainage device in dehumidification part, and using PID controllers to control key links. The actual application showed the system improves output power and generating efficiency of generating units and prolongs running time and life of the units compared with current gas-generation pretreatment system, which has higher safety and reliability.

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Endometrial carcinoma is a very heterogeneous gynecological cancer. According to the latest molecular classification, endometrial carcinoma is divided into mismatch repair deficiency (MMRd) -related EC and non-MMRd-related EC. And MMRd-related EC is divided into three subtypes according to the characteristics of mutation: MLH1-hypermethylationged endometrial carcinoma (EC-met); Lynch syndrome-related endometrial carcinoma (EC-ls) and mismatch repair gene double somatic variants (EC-dspv). The three subtypes of EC have different pathogenesis and clinical characteristics. Therefore, they must be distinguished according to clinical and immunological characteristics in order to adopt precise treatments. This article mainly introduces the classification of MMRd-related endometrial carcinoma, and compares the three subtypes with the mismatch repair proficiency (MMRp) of EC in terms of clinical and immune features. Finally, this review introduces recent researches and clinical trials related to the treatment and prognosis of MMR. [ABSTRACT FROM AUTHOR]

Cesarean scar pregnancy (CSP) is a special type of ectopic pregnancy that seriously threatens the life safety of women of childbearing age and leads to the loss of reproductive function. Uterine artery embolization (UAE) is an auxiliary method for the treatment of CSP, which can quickly block the blood supply to the lesion and reduce bleeding. Clinically, the combined scheme of UAE is often used to treat CSP, such as combined drug treatment (methotrexate, etc.), and surgical treatment (curettage, hysteroscopy, transabdominal, laparoscopic, transvaginal and other ways) to remove pregnancy. At present, for different types of CSP, how to choose the combination treatment scheme of UAE and the time to remove pregnancy while taking into account the cure rate and safety is a problem faced by clinicians. And there are no clear guidelines and unified standards for the treatment of CSP at home and abroad. This paper reviews the literature at home and abroad in recent years, and summarizes the effect of UAE combined with different treatment methods and the timing of removing pregnancy, in order to provide reference for follow-up research. [ABSTRACT FROM AUTHOR]

The incidence of endometrial cancer among Chinese women has increased year by year with a younger trend in morbidity age. Young patients with early endometrial cancer are increasingly demanding fertility preservation. At present, progesterone therapy is still the first choice of fertility-sparing treatment, and has achieved good results. However, progesterone resistance and adverse reactions have also become the main reasons for the failure of progesterone therapy in some patients. Therefore, understanding the mechanism of progesterone resistance and exploring coping methods has guiding significance in the fertility-sparing treatment for early endometrial cancer patients. This article reviews the mechanism of progesterone resistance, the new treatment methods to solve progesterone resistance (such as hysteroscopy), and the significance of molecular typing of endometrial cancer in predicting progesterone resistance [ABSTRACT FROM AUTHOR]

A 32-year-old patient(G3P1) was admitted to the hospital due to"fever accompanied by heart palpitations for 2 d" after 30+6 gestational weeks. Serum troponin I (cTnI) was 1.356 μg /L (↑), and creatine kinase MB(CK-MB) was 10.4 μg /L (↑). Electrocardiogram showed sinus tachycardia (heart rate 112 beats/min), and ST segment elevation (II, III, aVF and V3-V6 J point upward shift type). The patient had a history of "cold" 1 month ago. Among the multidisciplinary consultation, continuous renal replacement therapy and extracorporeal membrane oxygenation mechanical circulation support followed up. The patient was given caesarean section under general anesthesia after stable condition. After that, the patient was discharged from the hospital. There are few reports on pregnancy combined with fulminant myocarditis. The clinical data and treatment of this case can provide reference for clinicians. [ABSTRACT FROM AUTHOR]

A new method is proposed for mobile robot visual servoing using model-free visual targets under dynamic scenes. In the first stage, the ratio of depth information is calculated by using the geometric relationship between feature points and images. For the second stage, the relationship between various coordinate systems can be calculated via homography decompositions and coordinate transformation. Finally, an adaptive controller is adopted to drive the mobile robot to the desired pose. Both the simulation and experimental results show that when the scene changes, the mobile robot can complete the stabilization control. That is, when the linear velocity v→0 and the angular velocity w→0, the error e→0, then the robot stabilization to the desired pose 0.0 m, 0.0 m, 0.0°. [ABSTRACT FROM AUTHOR]

To improve predicting accuracy further, this paper modified original Pareto dominance relation among the candidate solutions, and proposed a new method named d-Pareto dominance prediction using nearest neighbor. Combined with the characteristics of multi-objective optimization, it described the framework of d-Pareto and the conclusion that a d-Pareto dominance prediction could obtain a lower average prediction error rate comparing with Pareto demonstrated dominance prediction. Besides, it also explored the interaction between d-Pareto dominance prediction and multi-objective evolutionary algorithms. Experiments on several classic MOPs were conducted and the simulation results show that prediction of d-Pareto dominance is feasible and effective. [ABSTRACT FROM AUTHOR]

The article presents a study which analyzes the probe-fed microstrip antennas in multilayered spherical media using Rao-Wilton-Glission (RWG) basis functions based on the method of moments (MoM).

A computer simulation model for EQ7200HEV hybrid car A was developed in the system simulation software MATLAB/Simulink environment. The bench test results for key components of the hybrid powertrain are treated as reliable reference in the modeling. Based upon the simple logic threshold approach in corporation of a stand-by optimization algorithm, a control strategy for the hybrid car A was proposed and also implemented in the real hybrid car. Simulation results were presented for behaviors of the engine, motor and battery, which demonstrate that the control strategy is effective. The present control strategy has been applied in the real car A and it is shown from the monitoring and testing that control functions are practical. [ABSTRACT FROM AUTHOR]

For the damping of speed oscillation of hybrid electrical vehicle (HEV) engine, this paper founded a PID controller according to the relationship between electrical throttle control (ETC) positions, engine speed and engine torque. The PID is implemented in the hybrid control unit and its parameters are tuned based on the engine response features. The experimental results prove that the overshoots and settles time decrease greatly with respect to the original one. [ABSTRACT FROM AUTHOR]

The effects of design parameters on the performance of an electric vehicle were presented. Based on the demands of the performance, a genetic-based methodology of optimizing the size of the principal hardware components of electric vehicle was presented. All the models of components are based on the bench test results of a practical configuration of the powertrain for QREV. Compared with the results of traditional optimization methods, the optimization results in this paper prove to be more valid, which can contribute to a substantial improvement of the performance. The optimization algorithm was applied in the real vehicle and it is shown from the design and testing that the optimization algorithm is practical. [ABSTRACT FROM AUTHOR]

Ecosystem structure determines material circulation, energy flow, and system function. Based on field investigation data in the Sanmen Bay, East China Sea from 2017 to 2018, ecological channel model was constructed by Ecopath, describing energy flow routes and functional characteristics of the Sanmen Bay ecosystem. Results showed that grazing food chain was the main energy circulation channel, and the trophic level of each functional group ranged from 1 to 3.80. Energy flow of the system was mainly concentrated in the first five levels, with an average energy conversion efficiency of 13.0%. Energy conversion efficiency was 12.8% and 14.5% from primary producers and debris, respectively. Connectance index (CI) and system omnivory index (SOI) were 0.40 and 0.24 respectively. Finn's cycling index (FIC) was 0.40, and Finn's mean path length (MPL) was 2.06. The ratio of total primary productivity to total respiration was 13.59. In conclusion, Sanmen Bay ecosystem was immature in material circulation and energy flow. This work is helpful to understand the structural and functional traits of coastal ecosystems in China.

Objective: To analyze the relationship between the expression level of SQLE and the prognosis of patients with acute myeloid leukemia (AML) through large sample data., Methods: The data of genome, transcriptome, gene chip expression, and clinical information were statistically analyzed in multiple cohorts of AML patients with large samples., Results: It was found that the expression level of SQLE gene in tumor cells of AML patients was significantly higher than that of healthy controls (P=0.001). In the three AML corhort, the SQLE high expression group showed a worse therapeutic outcome (OS, P=0.009, P=0.0001, P=0.006; EFS, P=0.005, P=0.001). The unvariate and multivariate survival prognosis analysis indicated that the high expression of SQLE suggests lower event-free survival rate (EFS, HR=1.551, P<0.05) and overall survival rate (OS, HR=1.484, P<0.05). At the same time, it was also found that among different risk subgroups, the expression of SQLE in high risk group was higher (P<0.001, P=0.01), while the patients with high SQLE expression, who received allogeneic HSCT, had longer overall survival time (P=0.006)., Conclusion: The up-regulation SQLE expression suggests a poor prognosis for the patients with AML.

In this study, we examined the effects of dazomet fumigation with different concentrations (0, 0.1, 0.2, 0.4 g·kg -1 ) on the microbial characteristics of continuous cropping soil and growth of Malus hupehensis seedling in greenhouse and open-field pot. The results showed that all the treatment of dazomet fumigation could promote the growth of M. hupehensis seedlings in continuous cropping soil, with 0.2 g·kg -1 treatment showing the strongest effect. Compared to the control, plant height, stem diameter, dry weight of M. hupehensis seedlings in 0.2 g·kg -1 dazomet fumigation were increased by 192.9% and 91.8%, 72.8% and 60.1%, 196.8% and 195.0%, 138.5% and 130.7%, respectively in greenhouse and open-field. The root related indices (root length, root area, root volume, root respiration rate) were significantly promoted. The activities of SOD, POD, CAT in roots were increased by 114.6% and 118.5%, 123.5% and 107.6%, 164.6% and 175.6% respectively compared with the control, whereas the content of malondialdehyde was significantly lowered. Soil bacterial content, fungal content, copy number of Fusarium oxysporum gene and soil enzyme activity were significantly decreased with the increasing dazomet concentrations. In conclusion, 0.2 g·kg -1 dazomet fumigation could increase the biomass of M. hupehensis seedlings in continuous cropping, improve soil environment, and effectively alleviate the continuous cropping obstacle. Therefore, 0.2 g ·kg -1 dazomet fumigation could be given priority during the reconstruction of old apple orchards.

Effects of fermented apple products on the growth of continuous cropping Malus hupehensis Rehd. seedlings and soil environment were examined in a pot experiment to provide theoretical basis for apple replant disease. There were four treatments, the replanted soil (control, CK), sterilized replant soil (T 1 ), replanted soil applied with apple fermentation products (T 2 ), and replanted soil applied with sterilized apple fermentation products (T 3 ). The results showed that T 1 , T 2 and T 3 significantly promoted seedlings growth, with better performance of T 1 and T 2 . T 1 increased root respiration rate, plant height, ground diameter, fresh weight, and dry weight by 107.3%, 50.6%, 42.4%, 171.7%, 225.3%, while T 3 increased them by 104.4%, 50.6%, 42.3%, 171.8%, 225.5%, respectively over CK. T 2 and T 3 increased the activities of nutrient conversion-related enzymes in continuous cropping soil. T 2 increased the activities of catalase, urease, neutral phosphatase and sucrase by 44.5%, 169.5%, 23.4%, 169.3%, while T 3 increased them by 23.7%, 72.6%, 1.5%, 121.5%, respectively. Catalase and sucrase activities under T 1 treatment did not differ from that in CK, whereas their urease and neutral phosphatase activities were reduced by 40.8% and 41.6%, respectively. The contents of ammonium, nitrate, available phosphorus and available potassium in T 2 soil were increased by 18.6%, 50.6%, 14.0% and 36.7% respectively. T 3 only increased the content of available nitrogen, with ammonium and nitrate being increased by 7.0% and 23.6% respectively. The content of available nutrients of T 1 decreased compared with CK. T 1 and T 2 significantly reduced the abundance of actinomycetes and fungi in soil and increased that of bacteria. The abundance of bacteria, actinomycetes and fungi in T 3 treatment were all significantly decreased. Results of real-time fluorescence quantitative PCR analysis showed that the gene copies of Fusarium proliferaturn, F. moniliforme, F. solani and F. oxysporum in T 1 , T 2 and T 3 were ecreased to different degrees. Apple fermented product could inhibit soil pathogen in replanted orchard soil, improve soil environment, and promote seedling growth, which could be used to alleviate the apple replant disease.

Objective: To investigate the clinical and prognostic value of SLC25A12 in patients with acute myeloid leukemia (AML)., Methods: The expression levels of SLC25A12 in bone marrow or peripheral blood cells of AML patients and healthy people in two independent cohorts (n=46, n=290, respectively) were compared. Then it was assessed that the prognostic value of SLC25A12 expression in two independent AML study cohorts (n=163, n=329, respectively) by mean of integrated analysis of genomic, transcriptome, clinical and prognosis information., Results: The expression of SLC25A12 in AML patients significantly increased as compared with that of healthy people (P=0.0001, P=0.0238, respectively). Univariate and multivariate analyze showed that high SLC25A12 expression was significantly associated with shorter event-free survival (EFS)(HR=1.605, P=0.018) and overall survival (OS)(HR=1.818, P=0.002) of patients. In favorable-risk and intermediate-risk subgroups, patients with high SLC25A12 expression showed shorter EFS and OS than patients with low SLC25A12 expression., Conclusion: High SLC25A12 expression significantly associated with poor prognosis of AML patients, which suggests that SLC25A12 aberrant expression can be used as a potential molecular marker for prognosis evaluation of AML patients.

Mytilopsis sallei, an invasive alien species, has strong reproductive ability and high adaptability. It can severely endanger biodiversity of intertidal ecosystem after invasion. The intertidal zones and oyster breeding areas in some coastal areas of Guangdong Province have been severely invaded by M. sallei. To examine the potential habitat of M. sallei in China, we established a potential habitat prediction model of M. sallei using Maxent and ArcGIS method for China and global scales. The model was verified by the method of receiver operating characteristic curve (ROC) analysis and field investigation. The results showed that M. sallei could distribute with high probabili-ty in the area between North and South America, South India in Asia, Sri Lanka, the south coast of the Yangtze River in China, and in Van Dimen Bay of the southern hemisphere. In China, M. sallei mainly distributed in coastal provinces south of Shanghai. The main environmental factors affecting the suitable distribution areas for M. sallei were water vapor pressure, temperature, and solar radiation. After ROC detection, the AUC values of both the training and testing sets were 0.996, indicating that the prediction reached an excellent level. Our results provide theoretical basis for the risk assessment and management of M. sallei, and complement the potential habitat prediction of invasive species in China.

Objective: To study the protective effects of azithromycin on renal damage induced by doxorubicin and albumin in mice., Methods: Forty male BALB/c mice were randomly divided into blank control group (Ctrl group), renal damage model group (ADR+BSA group), azithromycin treated group (Azm group) and prednisone acetate positive control group (Pdn group) in accordance with random number table method. Mice in ADR+BSA, AZM and Pdn group were injected intravenously with 9.8 kg -1 doxorubicin five days a week, 10 kg -1 serum albumin was injected intraperitoneally, and normal saline was administered to the control group for 4 weeks to establish renal damage model. After that, AZM group was given daily. 62.5 kg -1 azithromycin was intragastrically administered. The Pdn group was given 12.5 kg -1 prednisone acetate daily, the other two groups were given the same amount of normal saline. After 6 weeks, the urine volume was collected and recorded for 24 hours to detected urine protein amount and endogenous creatinine clearance rate (Ccr). Serum biochemical indicators and serum immune factors were detected., Results: Compared with the Ctrl group, the 24 h urine protein level of the ADR+BSA group was increased significantly (P＜0.05), and the Ccr was decreased significantly (P＜0.05). After the azithromycin treatment, the 24 h urine protein was decreased significantly (P＜0.05), while the Ccr was increased significantly (P＜0.05) compared with ADR+BSA group., Conclusion: Azithromycin has a protective effects on the renal damage induced by doxorubicin and albumin in mice.

Acephalic spermatozoa syndrome (ASS) is characterized by a predominance of headless spermatozoa with abnormal head-tail junction in the ejaculate, which causes severe male infertility. The pathogenic mechanism of ASS remained unclarified for a long time until recent identification of the four ASS-associated genes SUN5, PMFBP1, TSGA10, and BRDT and their mutations due to the development of high-throughput sequencing technology. This review summarizes the advances in the genetic studies of ASS, focusing on its pathogenic molecular mechanisms, which provide an important basis for the molecular diagnosis of the disease as well as for assisted reproductive technology.

Based on ship visas, lockage data, and automatic identification system (AIS) data, vessel emission inventories for inland rivers of Jiangsu Province were established by using a vessel power-based approach, and the characteristics of vessel emissions were also discussed. The results showed that NO x , SO 2 , PM 2.5 , PM 10 , hydrocarbons (HC), CO, and CO 2 emissions of inland ships in 2014 were 1.87×10 5 , 5.13×10 4 , 8.2×10 3 , 1.1×10 4 , 6.4×10 3 , 1.67×10 4 , and 1.05×10 7 t, respectively, for inland vessels (excluding the Yangtze River), and dry cargo ships had the largest pollutant emissions. The highest pollutant emissions were found in the tonnage range of 200-600 t, and the highest pollutant emissions were found during the normal navigation conditions of ships. For the arrival of ships in the Jiangsu section of the Yangtze River, the non-container cargo ships had the highest pollutant emissions, and the emissions of pollutants were the highest under loading and unloading conditions. Under a cruise state, the main engine and auxiliary engine were the main emission units for different power units; for transit ships in the Jiangsu section of the Yangtze River, non-container cargo ships had the highest pollutant emissions, followed by oil tankers. The highest emissions of all pollutants occurred under slow driving conditions, and for different power units, the emissions of SO 2 , PM 2.5 , and PM 10 from the main engine were higher than those from the auxiliary engine. The emissions of atmospheric pollutants along the channel length of the channel of the North Jiangsu section of the Beijing-Hangzhou Grand Canal were relatively large, followed by those of the South Jiangsu Channel. The inland ship emissions in Jiangsu Province were less affected by time, except for the slightly smaller proportion of emissions in February, and the proportion of emissions in other months was basically uniform, where values ranged from 8% to 10%.

The purpose of this study is mainly to have qualitative-quantitative analysis on the adulteration in rice bran oil by near-infrared spectroscopy analytical technology combined with chemo metrics methods. The author configured 189 adulterated oil samples according to the different mass ratios by selecting rice bran oil as base oil and choosing soybean oil, corn oil, colza oil, and waste oil of catering industry as adulterated oil. Then, the spectral data of samples was collected by using near-infrared spectrometer, and it was pre-processed through the following methods, including without processing, Multiplicative Scatter Correction(MSC), Orthogonal Signal Correction(OSC), Standard Normal Variate and Standard Normal Variate transformation DeTrending(SNV&#95;DT). Furthermore, this article extracted characteristic wavelengths of the spectral datum from the pre-processed date by Successive Projections Algorithm(SPA), established qualitatively classified calibration methods of adulterated oil through classification method of Support Vector Machine(SVM), optimized model parameters(C, g) by Mesh Search Algorithm and determined the optimal process condition. In extracting characteristic wavelengths of the spectral datum from pretreatment by Backward interval Partial Least Squares(BiPLS) and SPA, quantitatively classified calibration models of adulterated oil through Partial Least Squares(PLS) and Support Vector Machine Regression(SVR) was established respectively. In the end, the author optimized the combination of model parameters(C, g) by Mesh Search Algorithm and determined the optimal parameter model. According to the analysis, the accuracy of prediction set and calibration set for SVC model reached 95% and 100% respectively. Compared with the prediction of the adulteration oil content of rice bran oil which was established by the PLS model, the SVR model is the better one, although both of them could implement the content prediction. Furthermore, the correlation coefficient R is above 0.99 and the Root Mean Square Error (MSE) is below 5.55 x 10(-4). The results show that the near-infrared spectroscopy technology is effective in qualitative-quantitative analysis on the adulteration of rice bran oil. And the method is applicable to analyze adulteration in other oils.

Objective: To explore clinical effects of internal fixation in treating displaced clavicle fracture combined with coracoid process., Methods: From January 2005 to July 2012, 9 patients with displaced clavicle fracture combined with coracoid process were treated by internal fixation. Among them, there were 6 males and 3 females with an average age of 40.1 (ranged from 20 to 57) years old. According to Eyres classification: 3 cases were type II B, 1 case was type II A, 3 cases were type III B, and 2 cases were type V A. All patients had history of injury, and diagnosed as coracoid fracture X-ray and CT before operation. Herscovici criteria was used to evaluate function of shoulders joint after operation., Results: Seven of 9 patients were followed up from 6 to 18 (averaged 11) months. The incisions were healed at stage I, coracoid process obtained bony healing, and reduction of acromioclavicular joint well. According to Herscovici criteria, 6 patients got excellent results and 1 in good., Conclusion: Internal fixation for the treatment of displaced clavicle fracture combined with coracoid process could restore physiological anatomical position of coracoid process, and benefit for recovery of limb function.

Objective: To study the value of hCG ratio of blood in peritoneal cavity versus venous blood (RPhCG/VhCG) in diagnosis of early ectopic pregnancy (EP)., Methods: From Mar. 2009 to Oct. 2012, 268 cases with EP (EP group) and 53 women with intrauterine pregnancy with haemoperitoneum (hIUP) (hIUP group) from International Peace Maternity and Child Health Hospital of Shanghai Jiaotong University School of Medicine, Shanghai 6th People Hospital and Shanghai Jiangwan Hospital were enrolled in this prospective study. The HCG of all subjective were measured in blood in peritonea cavity and venous blood, then calculate the ratio of RPhCG/VhCG. Scatter point analysis and ROC were used to differentiate EP, determine threshold of hIUP and evaluate the sensitivity, specificity and accuracy in diagnosis EP preoperatively., Results: The mean RPhCG/VhCG of EP group was 4.35, which was significantly higher than 0.81 in hIUP group (P < 0.01). Scatter point analysis showed that the threshold value of RPhCG/VhCG between ectopic pregnancy and hIUP was 1.0. The overall sensitivity of RPhCG/VhCG in the diagnosis of EP was 98%, the specificity was 100%, the positive predictive value was 100% and the negtive predictive value was 93%., Conclusion: RPhCG/VhCG > 1.0 could be used to diagnose and differentiate EP from hIUP accurately.

Aim: To establish bacterial display technology for the purpose of Fab antibody library screening, by using six amino acids (CDQSSS) of the amino termimus of NlpA protein to anchore antibodies to the periplasmic side of the bacterial inner membrane., Methods: The NlpA Leader sequences (encoding CDQSSS) was amplified from pNAD plasmid. The PCR product was subcloned into pComb3 expression vector to generate Fab display vector pBFD. The heavy chains of the Fab gene fragments and the light chains of the anti-human IL-1β (hIL-1β) antibody were inserted downstream of the NlpA leader and pelB leader respectively to construct the pBFD-Fab for Fab antibody display. Then pBFD-Fab transformed E.coli DH5α was induced by IPTG to express the Fab antibodies, as detected by flow cytometry (FCM), and positive populations were sorted. Instead of PCR, plasmids were extracted for rescue purpose. The rescue plasmids were retransformed to E.coli DH5α and FCM was performed again., Results: The pBFD-Fab-transformed bacteria were incubated with antigen and antigen specific FITC-antibody, and showed strong fluorescence as detected by FCM in a dose-dependent manner. The rescued pBFD-Fab displayed similar fluorescence intensity, indicating the reliability of this technology., Conclusion: The Fab expressed by the bacterial display system folds efficiently and binds to hIL-1β specifically. The plasmid rescue works well and it can avoid mutation and mis-pairing chains. This bacterial display technology has the stability of antibody expression. This study has used the technology to screen anti-hIL-1β Fab antibody Library successfully.

The Shijiuyang ecological wetland for drinking water of Jiaxing City, Zhejiang Province is one of the biggest constructed wetlands for water resource protection in China. To ensure a deep understanding of the present status of the wetland vegetation of Shijiuyang ecological wetland which has been run for 2.5 years and provide support for the vegetation management of ecological wetland, systematic investigation was carried out by using plot method and quadrat method in October to November, 2010. The species composition, dynamics of plant diversity and the biomass production during operation were analyzed. Altogether 70 species belonging to 28 families and 62 genera were recorded. Among them, there were 26 wetland plants, 20 mesophytes, 14 emergent, 4 submerged, 6 floating ones. Compared with the preliminary stage, the species numbers of wetland plants increased significantly from 15 species to 70 species. The spatial pattern of riparian species diversity was examined by adopting the Simpson index and Shannon-Wiener index as species diversity indices. The results showed that the riparian species diversity was higher in the west of the Beijiaohe river (Simpson index = 0.468 3, Shannon-Wiener index = 0.835 2) than that in the south of the Dongsheng Road (Simpson index = 0.357 6, Shannon-Wiener index = 0.660 4). The analyses of quantitative characteristics of wetland vegetation showed that the plants in the root-channel purification zone in the south of the Dongsheng Road grew better than those in the west of the Beijiaohe river. With regard to the riparian vegetation, the riparian plants in the west of the Beijiaohe river were more abundant. The mean biomass production (dry weight) in the root-channel purification zone was 1.73 kg x m(-2) and the total area was 9.12 x 10(4) m2, so the total biomass production was estimated to be 157.8 t. In the same way, the mean riparian vegetation biomass production(dry weight) was 0.83 kg x m(-2) and the total vegetation area was 3.75 x 10(4) m2, so the total riparian vegetation biomass production(dry weight) was estimated as 31.1 t.

The development characteristics and improvement measures of aquatic plants were studied in Shijiuyang Constructed Wetland (SCW) at its initial operation stage. SCW was a large-scale wetland aiming to help relieve the source water pollution in Jiaxing City. A checklist of vascular plants in SCW was built, and species composition, life forms, biomass and association distributions were examined. Our objectives were to examine the diversity and community structure of aquatic plants in SCW at its initial operation stage, and to find out the possible hydrophyte improvement measures. The survey results showed that there were 49 vascular plant species belonging to 41 genera, 25 families in SCW, which greatly exceeded the artificially transplanted 13 species. The life forms of present aquatic plants in SCW were dominated by hygrophilous plants (20 species) and emerged plants (17 species), which accounted for 75.5% of the total number of aquatic plants. The aquatic plants transplanted artificially were dominated by emerged plants (accounted for 69.2%), while those naturally developed were predominated by hygrophilous plants (accounted for 47.2%). The horizontal distribution of aquatic plant community in SCW was mixed in the form of mosaics, which made up typical association complex. Except association Aeschynomene indica L., the dominant species of other associations were all those transplanted artificially. The naturally grown species scattered throughout the SCW and only occupied a small percentage. A marked difference was detected on the species and species richness of aquatic plants in different regions of SCW. Biomass of aquatic plant associations in SCW was 167.7 t. SCW has shown a trend of succession heading for quick increase of plant diversity at the primary operation stage. This trend provides a good material base for the future stable community of aquatic plants in SCW. According to the current status of aquatic plants, some suggestions were put forward on the further optimization and utilization of aquatic plant systems in SCW.

Aim: Design and synthesis complementary DNA sequences of 3 pairs of short hairpin structure and a pair of negative control sequence by RNA interference technique and construct and identify a lentiviral interference vector with human BIRC5 gene as target gene., Methods: The designed and synthesised Single-Stranded primer were annealed to Double-Stranded oligo sequences and subcloned into linear pMAGic lentiviral plasmid vector digested by enzyme Age I and EcoR I. Screening positive clone after transformed into DH5α competent cells and identified by PCR amplification and DNA sequencing., Results: 335 bp straps of positive clone and 298 bp straps of negative clone form PCR amplification production have been obtained after gel electrophoresis, the designed and synthesised sequences have been contained in these clone straps confirmed by the result of DNA sequencing., Conclusion: Four pairs of BIRC5 shRNA recombinant lentiviral expression vector were constructed successfully, which laid the foundation for researching the inhibition of BIRC5 siRNA target against tumor cells proliferation, induction apoptosis and gene therapy.

Runoff samples were collected from 14 source areas in Hanyang district during four rain events in an attempt to investigate the spatial differentiation and influencing factors of urban stormwater runoff quality. The outcomes are expected to offer practical guidance in sources control of urban runoff pollution. The results revealed that particle-bound proportion of chemical oxygen demand (COD), total nitrogen (TN), and total phosphorus (TP) in stormwater runoff were 58% +/- 17%, 65% +/- 13% and 92% +/- 6%, respectively. The fractions of ammonia, nitrate and dissolved organic nitrogen were homogeneous in dissolved nitrogen composition. Urban surface function, traffic volume, land use, population density, and street sweeping practice are the main factors determining spatial differentiation of urban surface runoff quality. The highest magnitude of urban stormwater runoff pollution was expected in the old urban residential area, followed by general residential with restaurants, commercial and transport area, new developments and green land. In addition, the magnitude of road stormwater runoff pollution is positively correlated to traffic volume, in the following order: the first trunk road > the second trunk road > minor road. Street sweeping and critical source areas controls should be implemented to mitigate the adverse effects of urban stormwater runoff on receive waters.