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Objective To investigate the mental health status of eight-year medical undergraduates in different academic years and to analyze the influencing factors, so as to put forward countermeasures to improve the mental health of students. Methods Questionnaires were used to investigate the mental health status of undergraduate medical students in grades 5 to 7 in our university. The differences among groups were analyzed according to different grades. The statistical methods of Chi-square test and Fisher exact test were used for data analysis. Results The recovery rate of the questionnaire was 96.5%, and 164 qualified questionnaires were obtained. Fourteen students (8.5%) had depression and 28(17%) had anxiety problems. Fifty-seven cases (34.76%) needed the help of self-rating questionnaire and 110 cases (67.07%) needed the help of depression and anxiety counseling. The proportion of demand for depression/anxiety counseling among sixth grade medical students was significantly higher than that of fifth grade (82% vs 50%; P＜0.05) and seventh grade (82% vs 57%; P＜0.05). As much as 86.67% of the seventh grade students had self-rating psychological questionnaires, higher than the fifth grade group (64.29%, P＜ 0.05) with no significant difference with the sixth grade group. Conclusions The mental health status of eight-year medical students in different learning stages is different, and there are emotional adjustment disorders different degrees. Effective measures they need to support and psychologically intervention.