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Objective To investigate the effect of moxibustion based on Lai's Tongyuan needling technique on retention of urine after stroke. Methods Totally 65 stroke patients with retention of urine were randomly divided into the control group (n=32) and observation group (n=33). All patients received comprehensive treatment and nursing care, and those in the observation group were given moxibustion based on Lai's Tongyuan needling technique. The frequency of urination and residual urine volume were recorded, and the effect of intervention was compared between two groups. Results Better outcomes of frequency of urination and residual urine volume were found in the observation group than those in the control group after intervention(P＜0. 05), The overall effective rate was 96. 67%(32/33) in the observation group and 78. 13%(25/32) in the control group, with a significant difference (P＜0. 05). Conclusion The moxibustion based on Lai's Tongyuan needling technique is potentially effective to improve the bladder function of patients with retention of urine after stroke. (目的 观察赖氏通元法艾灸对中风后并发癃闭患者的护理效果。方法 选择中风后并发癃闭的患者65例, 按随机数字表法分为对照组32例和试验组33例, 对照组采用常规治疗与护理, 试验组在对照组基础上采用赖氏通元法艾灸。记录2组患者排尿次数和残余尿量, 评价干预效果。结果 试验组排尿次数、膀胱残余尿量改善情况优于对照组, 差异有统计学意义(P＜0. 01)。试验组总有效率96. 67%(32/33), 高于对照组的78. 13%(25/32), 差异有统计学意义(P＜0. 05)。结论 赖氏通元法艾灸可改善中风后癃闭患者的膀胱功能。)

Objective: We studied the interactions in a co-culture of two bacteria., Methods: By pairwise co-culturing of 36 Escherichia coli and 36 Staphylococcus aureus strains, we monitored the growth of each species in an interaction environment. We identified numerous Single Nucleotide Polymorphisms (SNPs) by whole-genome sequencing used as genetic markers to predict variations in phenotypic traits. Genome-wide association study (GWAS) was applied to identify loci that controlled competition between the two species., Results: In E. coli, 162 significant SNPs affected the change of maximum growth rate by comparing initials strains with those grown in co-culture, and 36 significant SNPs affected the change of maximum growth rate comparing monoculture and co-culture strains. Five of the significant E. coli genes we identified after annotation this time were also reported in other evolutionary studies. We also identified 85 significant SNPs in S. aureus that affected the change of maximum growth rate by comparing initial strains with those grown in monoculture. About the change of bacterial numbers, we found that 706 significant SNPs were associated in E. coli and 129 in S. aureus. Thirteen of the E. coli significant genes in this study were also verified in previous evolutionary reports, Conclusion: We found several significant genes both in monoculture and co-culture affecting the interaction of E. coli and S. aureus. GWAS has the potential to study interspecific interactions of bacteria.