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Objective: To investigate the lipidomics differences of CD4+T immune cells in diabetic kidney disease (DKD) mice, and screen out the differential metabolites with biological significance. Methods: CD4 (L3T4) MicroBeads immunomagnetic beads were used to isolate CD4+T immune cells from spleen of BKS. Cg-Dock7m+/+ Leprdb/J mice with spontaneous DKD; the purity of CD4+T cells were identified by flow cytometry. The non-targeted lipidomics of CD4+T cells were detected by LC-MS/MS, and the differential metabolites were analyzed. Results: A total of 463 metabolites were detected by LC-MS. PCA and OPLS-DA analysis showed that the metabolic components were significantly separated; twenty-four differential metabolites were screened out. KEGG and enrichment analysis showed that the differential metabolites involved in the disorder of glycerol phospholipid metabolism. Conclusion: Phospholipid metabolism of CD4+T cells is closely related to the occurrence of DKD. Phospholipid metabolism targeting DKD CD4+T cells in DKD may be a new direction of DKD treatment. [ABSTRACT FROM AUTHOR]