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The study on stem cells is a hot field in biomedical science in recent years, and has furthered from laboratory to clinical application. Stem cells, according to their developmental stage and differential properties, can be divided into embryonic stem cells, induced PS cells and adult stem cells, among which, adult stem cells have already been applied to the clinical treatment of many systemic diseases. Currently, the study of spermatogonial stem cells and adult stem cells is in the front of the basic researches on the treatment of male infertility, but the time has not yet arrived for their clinical application. This paper outlines the application prospect of adult stem cells in male infertility.

To improve the accuracy of the transient temperature detection system, transient temperature inversion processing algorithms was proposed based on spectrum analysis of speckle pattern interferometry. The interference fringes were formed by speckle interferometry in the system, and due to transient temperature changes that cause the material strain, the speckle interference pattern changes. The interference fringes on the measured surface were obtained by the area array CCD collection before and after deformation. The corresponding spectrum density function will change with the changes in the transient temperature, and the amplitude changes of center wavelength were inverted by the speckle pattern interferometry. Through detecting and calculating the ratio of the amplitude of the center wavelength, the transient temperature can be obtained by spectrum analysis. In the analysis and calculation for the function of transient temperature and material strain, material strain and interference fringes, the amplitude and phase function of the transient temperature change and interference fringes were derived, providing the necessary conditions for detecting spectral density function temperature. The experiment used 660 nm laser diode and SI6600 type area CCD detector. By extracting the offset of the center wavelength from the spectrum distribution function, the calculation and calibration data were compared to the data obtained with the traditional method of interference temperature detection, and the result showed that the detection accuracy can achieve 0.3%. Compared to traditional direct detection of interference fringes changes, the accuracy improved nearly three times by the method.

Objective: To obtain human mitochondrial transcription factor A (TFAM), mitochondrial transcription factor B1 (TFB1M), and mitochondrial transcription factor B2 (TFB2M) that were expressed efficiently in E. coli BE21 and to purify the target proteins., Methods: TFAM, TFB1M, and TFB2M segments were designed and synthesized. After having been sequenced, the reconstructed expression vectors were constructed by enzyme digestion and by cloning into an expression vector pET42a. Then the reconstructed vectors were transformed into E. coli BL21. Recombinant glutathione S transferase (GST) fusion proteins were expressed via the induction of IsoPropyl beta-D-ThioGalactoside (IPTG) and purified by glutathione Sepharose 4B., Results: The expression plasmids of pET42a-TFAM, pET42a-TFB1M, and pET42a-TFB1M were successfully constructed. The sequences of the cloned gene segments were identical with GenBank reported. The protein bands with relative molecular masses of 56 000, 67 000, and 69 000 appeared on sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) after the expressed GST-TFAM, GST-TFB1M, and GST-TFB2M fusion proteins were separated by SDS-PAGE. The expressed fusion proteins were purified to high purity., Conclusion: The recombinant plasmids pET42a-TFAM, pET42a-TFB1M, and pET42a-TFB2M were successfully constructed, and the GST-fused target proteins were prepared.

The bovine genomic DNA was extracted from bovine blood, then bovine mammary gland matrix attachment region (BMARs) was cloned using a pair of primers, which were designed based on the related sequences in GenBank through bio-software Primer5.0 and Vector7.0. Upon preliminary analysis with bio-software, BMARs was TA cloned into PMD-18 T vector. By means of adding Kpn2 I and Xho I to 5' upstream of sensitive and antisensitive primers respectively, expressing vector BE was constructed after BMAR was cloned into the downstream of the reporter gene in pEGFP-C1. Bovine ear fibroblast cells were transfected by expressing vector BE with Lipofectamine.Compared with control bovine ear fibroblast cells transfected with pEGFP-C1, the effect of cloned BMR was apparent in dispelling position effect and enhancing gene expression.