Search

Background Opportunistic salpingectomy (OS) can be used as a primary prevention of ovarian cancer (OC) , but OS may cause impairment of ovarian function due to the homologous blood supply system shared by ovaries and fallopian tubes, thereby increasing the risk of ovarian aging and early menopause. Objective To evaluate the short-term effects of OS on ovarian reserve based on GRADE evidence grading system. Methods In September 2022, CNKI, Wanfang, VIP, PubMed, Web of Science, and Scopus were searched for literature assessing the short-term effects of OS on ovarian reserve from inception to 10 September 2022. Two investigators independently screened the literature and extracted the data. The Jadad scale was used to evaluate the quality of literature, Stata 17.0 software was used for data processing and meta-analysis, and GRADEpro 3.2 software was used to assess the evidence quality for the results of meta-analysis. Results A total of 9 randomized controlled trials (RCT) were included, involving 482 patients, including 238 cases in the OS group and 244 in the non-OS group. The results of the Jadad risk assessment scale showed that the included RCTs were all of high quality literature. The results of meta-analysis showed that the differences in changes of anti-mullerian hormone (AMH) (WMD=-0.07, 95%CI=-0.28-0.13, P=0.13) , follicle-stimulating hormone (FSH) (WMD=-0.03, 95%CI=-1.65-1.59, P=0.24) , luteinizing hormone (LH) (WMD=-0.39, 95%CI=-1.62-0.83, P=0.08) and estradiol (E2) (WMD=3.08, 95%CI=-4.26-10.43, P=0.35) before and after surgery between the OS and non-OS groups were not significant (P>0.05) . The results of the meta-analysis by GRADEpro software showed high quality evidence for AMH, moderate quality evidence for FSH, and low quality evidence for E2 and LH. Conclusion There is no significant difference in ovarian reserve indicators between the non-menopausal women who receive OS for benign disease in the short term and those who did not receive OS. It is reasonable for premenopausal women who have completed childbearing to prevent OC by OS, but this conclusion remains to be validated by a longer follow-up and more rigorous RCTs with a larger sample size.

BACKGROUND The Xianghualing mining area in Hunan Province is an important part of the east-west tectonic-magmatic-metallogenic belt in the Nanling area.The ore-forming geological conditions in the mining area are superior, and it is famous for its many types of deposits and complex types of minerals.The tin-lead-zinc deposit in the Xianghualing mining area is a very important polymetallic deposit in the Nanling metallogenic belt.The ore types are mainly cassiterite sulfide tin ore, tin-lead-zinc ore and sulfide lead-zinc ore.Tin, lead and zinc are very important nonferrous metal elements.The abundance and variation characteristics of these three elements can reflect the regional metallogenic conditions, indicate the existence of deposits or mineralization, and are the important basis for studying the metallogenic model, deepening the understanding of the genesis of the deposit, and identifying the metallogenic control factors.Therefore, it is very necessary to accurately determine the content of tin, lead and zinc in the tin, lead and zinc deposits in the Xianghualing mining area, and it is important to study the regional metallogenic conditions The geological characteristics of the deposit, the occurrence state of elements, mineral processing and comprehensive utilization of non-ferrous metal minerals are of great significance.However, the average grades of tin, lead and zinc in the deposit are percentage contents and tin itself is a refractory element.Conventional open acid dissolution or closed acid dissolution in the dissolution of high content of tin, lead and zinc in the ore will have the disadvantages of incomplete digestion and easy precipitation of the solution, resulting in serious low determination results.However, alkali fusion is more capable of decomposing minerals than acid dissolution.As long as the appropriate alkali fusion reagent, temperature and time are optimized, some high content of inorganic elements, even insoluble elements, can be completely decomposed, which is an ideal method of mineral decomposition pretreatment. OBJECTIVES To rapidly and accurately determine the concentrations of tin, lead and zinc in high contents of tin, lead and zinc samples of a tin-lead-zinc deposit in the Xianghualing mining area, Hunan Province. METHODS Inductively coupled plasma-optical emission spectrometry (ICP-OES) with alkali fusion was used to determine the levels of tin, lead and zinc.The selection of alkali fusion reagent, reagent dosage, alkali fusion temperature and time as well as the plasma excitation conditions, element spectral lines, button background position and other instrumental determination conditions were optimized.The effects of three kinds of fluxes: sodium hydroxide, anhydrous sodium carbonate and sodium peroxide were compared. RESULTS The measured value of tin was greatly affected by the alkali flux.Under the flux of sodium hydroxide or anhydrous sodium carbonate, the measured value of tin was low, while the measured value of tin was consistent with the standard value under the flux of sodium peroxide.Based on the comparison of the effects of various fluxes on the analysis results, sodium peroxide was selected as the flux, melting at a constant temperature of 750℃ for 20 minutes, and acidification with 20mL of hydrochloric acid after 30mL of boiling water extraction to ensure the complete decomposition of the sample.The calibration series was prepared with blank alkali melt solution as the medium, so that the calibration series matched the sample matrix and eliminated the influence of matrix interference.The national first-class geochemical reference materials were used for method quality evaluation, and the results showed that the absolute value of the logarithmic difference between the measured value and the certified value of the reference material was less than 0.04.The detection limits of the method for tin, lead and zinc were 13.60μg/g, 36.45μg/g and 53.83μg/g, respectively.The precision of the method was better than 8%.The determination range of the calibration curve was between 0 to 100μg/mL. CONCLUSIONS Due to the use of alkali fusion method and blank alkali fusion solution as the standard preparation medium, this method is suitable for the determination of high contents of tin, lead and zinc in cassiterite-sulfide type tin ores, tin-lead-zinc ores, and sulfide type lead-zinc ores, and it has good application prospects.At present, this method has been successfully applied to the analysis of actual samples in the Xianghualing mining area, Hunan Province, with satisfactory results.

A liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was established for the simultaneous determination of 35 antibiotic residues of tetracyclines, sulfonamides, penicillins, macrolides and amphenicols in milk. The samples were extracted with alkaline acetonitrile and McIlvaine buffer solution under ultrasonication. The separation of target compounds was performed on an Eclipse XDB-C, column (150 mm x 2.1 mm, 3.5 µm) with gradient elution at a flow rate of 0.25 mL/min, and with an injection volume of 10 µL. The identification and quantification of the compounds were completed by liquid chromatography-tandem mass spectrometry in multiple reaction monitoring ( MRM) mode. The limits of detection were all below 10.0 µg/kg. The average spiked recoveries of the method ranged from 70. 1% to 109. 9% with relative standard deviations (RSDs) of 2.89%-9.99%. After validation, the method was applied to the analysis of antibiotic residues in milk products in China. Fifty samples were screened under the well defined methodology, and the results showed that chloramphenicol, only in one sample, was monitored with the content of 0.48 µg/kg. A risk of contamination of milk with chloramphenicol has been determined to exist. Therefore this method is convenient, rapid, sensitive and reliable, and can be successfully applied to the simultaneous detection of the 35 antibiotic residues of tetracyclines, sulfonamides, penicillins, macrolides and amphenicols in milk.

Objective: Our aim was to know response of spring bacteria and metabolic characteristics of sensitive bacteria to felt earthquake., Methods: Water samples were collected from January 31 to December 31, 2012, during which period 5 felt earthquakes occurred and the epicenter was 100 kilometers away from the No. 10 Spring in Urumqi. We monitored the spring bacterial activities and function diversity changes from No. 10 Spring in Urumqi during the pre- and post-earthquake stages by using plate culture counting methods and BIOLOG GEN III bacteria plate., Results: The spring bacterial numbers presented stochastic dynamic changes through the year. The culturable bacteria numbers and average well color development (AWCD ) of carbon source utilization of bacterial community were higher after the earthquake. Besides, there were some correlations with magnitude and epicenter distance of earthquake. The main carbon source utilization types of sensitive bacteria group for felt earthquake were sugar alcohol at the No. 10 Spring., Conclusion: The results indicated that the BIOLOG GEN III plate can be used for spring bacterial metabolism diversity research. Culturable bacteria numbers and carbon source utilization of bacterial communities showed some reflecting earthquake law.

Objective: To investigate the effect and medical cost of different revascularization strategies for acute myocardial infarction (AMI) patients with multi-vessel disease (MVD)., Methods: A prospective randomized controlled trial (RCT) was conducted. From January 2009 to June 2012, patients with AMI and MVD undergoing primary percutaneous coronary intervention (PCI) were enrolled. They were randomly assigned to group A [staged PCI for non-infarction related artery (non-IRA) within 7-10 days after AMI] and group B (subsequent PCI for non-IRA recommended only for those with evidence of ischemia). All of patients were given optimized medical therapy according to clinical guideline, and they were followed up for 24 months at regular intervals. Major adverse cardiovascular events (MACE) including recurrence of myocardial infarction and death due to cardiac ailments were recorded. Meanwhile, re-hospitalization from cardiac causes, recurrence of angina, heart failure, and re-PCI, number of stents, total hospital stay days, and total medical expenditure were recorded., Results: A total of 428 patients accomplished the 24-month follow up. All the patients underwent PCI for non-IRA in group A (215 patients), while 62 patients in group B (213 patients) undergone PCI for myocardial ischemia, and 51 patients received non-IRA treatment. There was no significant difference in MACE incidence between group A and group B [8.4% (18/215) vs. 10.8% (23/213), χ² = 0.727, P = 0.394]. The difference of death rate due to cardiac causes (5.1% vs. 6.6%), recurrence of myocardial infarction (4.2% vs. 6.6%), and heart failure (4.2% vs. 7.0%) were not significantly different between groups A and B (all P > 0.05). The rate of recurrence of angina (14.4 % vs. 32.9%), re-hospitalization from cardiac causes (14.4% vs. 33.8%), and re-treatment of implanting stents (12.6% vs. 29.1%) were significantly lower in group A than group B (all P < 0.01), and the rate of revascularization was significantly higher in group A than group B (10.7% vs. 5.2%, P < 0.05). The total number of stents (610 vs. 366), mean number of stents per patient (2.83 ± 0.91 vs. 1.72 ± 0.91, t = 12.725, P = 0.000), and total cost per patient (kRMB: 63.7 ± 12.6 vs. 51.5 ± 1 2.3, t = 10.107, P = 0.000) in group A were significantly higher than those in group B. Total hospital stay days in group A was significantly less than group B (days: 8.21 ± 2.45 vs. 9.89 ± .23, t = 6.071, P = 0.000). Because non-IRA-vascular reconstruction rate was low in group B, the rate of using β-blocker and anti-anginal agents during the 24-month follow up in group B was significantly higher than group A [59.2% (126/213) vs. 47.0% (101/215), χ² = 6.371, P = 0.012; 56.3% (112/213) vs. 17.6% (36/215), χ² = 64.704, P = 0.000]., Conclusions: In patients with AMI and MVD undergone emergency PCI, staged PCI within 7-10 days for non-IRA cannot decrease the incidence of myocardial infarction and death due to cardiac causes, recurrence of angina and rehospitalization for cardiac causes was diminished, and it may increase the number of stents and medical cost significantly.

Objectives: To evaluate the value of narrow band imaging (NBI) cystoscopy in detection of bladder cancer., Methods: Literatures on narrow-band imaging cystoscopy in diagnosis of bladder cancer, controlled clinical research was searched in PubMed, Cochrane Library,EMbase, and the Chinese Biomedical Literature Database. The literatures were selected according to the inclusion and exclusion criteria. The Meta-DiSc 1.4 software was used to review management and analysis., Results: The 8 studies met the inclusion criteria. On a per-people analysis, the pooled sensitivity, specificity, positive likelihood ratio, negative likelihood ratio, diagnostic odds ratio (DOR) of NBI cystoscopy and white light imaging (WLI) cystoscopy were respectively 0.943 (95%CI: 0.914-0.964) and 0.848 (95%CI: 0.803- 0.885), 0.847 (95%CI: 0.812-0.878) and 0.870 (95%CI: 0.831-0.903), 7.038 (95%CI: 3.357-14.754) and 6.938 (95%CI: 2.052-23.465), 0.054 (95%CI: 0.012-0.237) and 0.181 (95%CI: 0.091-0.361), 185.32 (95%CI: 45.714-751.260) and 42.931 (95%CI: 8.088-227.880). The areas under the curve (AUC) and Q* of NBI cystoscopy and WLI cystoscopy were 0.978 and 0.894, 0.934 and 0.825 respectively., Conclusion: NBI cystoscopy is accurate with high diagnostic precision for diagnosis of bladder cancer. NBI cystoscopy is prior to WLI cystoscopy, but it needs more clinical evidence for further affirmance.

Objective: We surveyed the composition and diversity of uncultured archaea in Xinjiang Dunbasitawu salt Lake sediment., Methods: Environmental total DNA was directly extracted from the sediment. We constructed clone library of 16S rRNA gene amplified with archaea-specific primers. A total of 59 positive clones were randomly selected from the library and identified by restriction length polymorphism (RFLP) with enzyme Hae III. Clones with the unique RFLP pattern were sequenced, and then by phylogenetic analysis., Results: The clone coverage C value was 89%, and Shannon-Wiener index was 2.69. In total, 21 Operational Taxonomic Units (OTUs) were obtained and affiliated with Euryarchaeota (92%) and Crenarchaeota (8%). The most of clones were affiliated to Halobacterium (24%), Haloarcula (18%), Natronorubrum (14%), and Halorubrum (8%), which belonged to family Halobacteriaceae (88%) with high similarity to that from thalassohaline environment. In addition, 11% of clones had less than 97% similarity with archaea sequences deposited in GenBank database., Conclusion: Compared with other similar Hypersaline environments, archaea diversity in Dunbasitawu salt lake was a little lower. The proportion of archara was different, but the composition is consistent. It was implied that some potential new species or lineages maybe exist in Dunbasitawu salt lake.

Objective: In order to investigated composition and diversity of bacterial in a cold sulfur spring in Xinjiang faulting zone., Methods: Environmental total DNA was directly extracted from the water of the No. 10 cold sulfur spring. The 16S rRNA genes were amplified from the total DNA by PCR with bacteria-specific primers and construction a clone library. Positive clones were randomly selected from the library and identified by restriction fragment length polymorphism (RFLP). The unique RFLP pattern corresponded sequences were sequenced, BLAST and then constructed phylogenetic tree., Result: In total, 228 positive clones were screened and grouped into 33 Operational Taxonomic Units (OTUs). The clone coverage C value was 92%. 33 Operational Taxonomic Units were divided into 3 phyla with Blast analysis and RDP classifer: Proteobacteria, Bacteroidetes, and Firmicutes. Proteobacteria (98%) was the absolutely dominant group, of which 20% of the clones were highly related to the known photoautotrophic and chemoautotrophic bacteria ( > 97% sequence similarity). Besides, 64% of the clones showed less than 96% of sequence similarity with sequence deposited in GenBank database, of them 54% sequences were affiliated to genus Legionella spp., Conclusion: Bacterial diversity in No. 10 cold sulfur spring was low, but maybe have a diversity of novel species and lineages. In addition, large number of novel species of Legionella were detected in the spring water may suggest the water potentially a source of Legionnaires disease and may constitute a menace to the health of human and livestock that lived down the spring.

An analytical method based on high performance liquid chromatography with a diode array detector (HPLC-DAD) has been established for the simultaneous determination of 3 benzalkonium chloride homologs (n-C12H25-C9H13NCl, n-C14H29-C9H13NCl, n-C16,H33-C9H13NCl) in cosmetics. The sample was extracted with methanol (including 0.5% formic acid) under ultrasonic operation, the HPLC separation was carried out on a CAPCELL PAK SCX column (250 mm x 4.6 mm, 5 microm), the mobile phases were 40 mmol/L ammonium acetate solution (including 0.1% triethylamine) and acetonitrile with gradient elution, the flow rate was 1.0 mL/min, the detection wavelength was 260 nm, the column temperature was 25 degrees C, and the injection volume was 20 microL. The limit of detection was 50.0 mg/kg and the quantitation limit was 200.0 mg/kg for 3 benzalkonium chloride homologs in cosmetics. The linear plots were obtained between 5.0 mg/L and 3000.0 mg/L. Overall recoveries were between 92.5% and 102.1% with the relative standard deviations (RSDs) between 3.81% and 6.66%. The method is simple, rapid, accurate and suitable for the determination of 3 benzalkonium chloride homologs in cosmetics.

Objective: We investigated endophytic bacterial diversity in Glycyrrhiza inflata Bat. from Xinjiang., Methods: We investigated endophytic bacterial diversity in root of Glycyrrhiza inflata Bat. from Xingjian by culture-independent method. Total DNA genome of Glycyrrhiza sample was extracted using CTAB (Hexadecyl trimethyl ammonium Bromide) procedure with some modifications. A pair of bacterial PCR primers were used for endophytic bacterial 16S rDNA gene amplification and a clone library was constructed for the Glycyrrhiza DNA samples. Clones screened from clone library on the basis of Hae III digestion patterns were sequenced and compared, flowed by constructing Neighbor-Joining tree., Results: In total 150 clones were grouped to 32 operational taxonomic units, most of the clones showed high similarity to the known cultured bacteria. Sequence analysis revealed diverse phyla of bacteria in the 16S rDNA library, which consisted of alpha, gamma subclasses of the Proteobacteria, Bacteroides, Firmicutes, Actinobacteria, and Uncultured bacteria; 74% of the clones were highly related to the known bacteria in the genus Sphingobium, Phyllobacterium, Hyphomonas, Agrobacterium etc (> 96% sequence similarity); whereas 26% of the clones showed lower affiliation with known genus (< 96% sequence similarity) and may represent novel taxa., Conclusion: There was abundant endophytic bacterial diversity in Glycyrrhiza inflata Bat. from Xinjiang as well as many unknown organisms.

Objective: To know halophilic actinomycetes and their enzyme-producing ability from mud volcano in Usu County, Xinjiang, China., Methods: Soil samples in mud volcano were isolated with five isolation media containing 5% and 10% NaCl (w/v) with dilution-plate method. The activities of lipase, galactosidase, amylase, esterase and cellulase from isolated strains were qualitatively detected by using five selective media. Base on morphological characteristics, test of salt tolerance, screening of enzymatic characters and sequencing of 16S rDNA gene, strains were selected for phylogenetic analysis., Results: A total of 43 halophilic actinomycetes and 3 extreme halophilic actinomycetes were obtained. Screening results for enzyme activity showed that 4 halophilic actinomycetes produced lipase, 30 strains produced galactosidase, 27 strains produced amylase, 6 strains produced esterase, 4 strains produced cellulose and 1 strain produced 4 enzymes simultaneously. According to 16S rDNA sequence analysis, 24 of 25 detected sequences were affiliated with Nocardiopsis, and the other one was Streptomyces. In the phylogenetic tree, strain 10006 producing 2 enzymes and Nocardiopsis exhalans (AY03600) showed 96.64% similarity (less than 97%), which indicated that strain 10006 was a possible new species., Conclusion: There are plenty of galactosidase or amylase-producing halophilic actinomycetes and higher enzyme-producing diversity of Nocardiopsis in Usu mud volcano. In addition, there are potential microbial resources in this extreme environment.

Objective: To study the feasibility of using mice marrow stromal stem cells(MSCs) as seed cells for tissue engineering cartilage to embed the seed cells in acellular cartilage matrix of human auricle., Methods: Acellular cartilage matrix was made from human auricle cartilage. The MSCs were isolated from the nucleated cells fraction of mice marrow by centrifuge. The MSCs were embedded in acellular cartilage matrix. After 10 day's combined culture, the specimens were observed with optical and electrical microscope., Results: The MSCs could well proliferate in the acellular cartilage matrix. The cells were not well-distributed in acellular cartilage matrix. There were more cells in the peripheral part of the matrix than in the central part of the matrix. Most of the cells were in cartilaginous lacunae. There were 1 or 2 cells in every cartilaginous lacunae., Conclusion: The MSCs can be used as seed cells of tissue engineering and can well proliferate in the acellular cartilage matrix and become tissue engineering cartilage.