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Silane coupling agent can not only improve the water resistance and corrosion resistance of the coating, but also improve the compatibility between the filler and the matrix resin. Water-borne damping coatings were prepared by introducing hexyltrimethoxysilane (HTMS) into a homemade dangling chain water-borne polyurethane-acrylic resin (DC-500-WPUA) system and adding sericite, thickener, and defoamer. The effects of silane coupling agent on coating properties were studied by changing the amount of HTMS added. Rheological analysis shows that the coating is pseudoplastic fluid. The introduction of HTMS can effectively improve the yield value of the coating, and the maximum yield value is 5.67 Pa, indicating that the coating has excellent resistance to current hanging. The introduction of HTMS increased the interaction between sericite and base resin, thus improving the heat resistance, adhesion, pencil hardness, and acid and alkali resistance of the film. The effective damping temperature range of sample 4 is as high as 125.88 °C (-31.21~94.67 °C), and the surface water contact angle is up to 122°, indicating that the film has excellent damping properties and water resistance. [ABSTRACT FROM AUTHOR]

To improve the electrical properties of CaMnO3 powders, the modified Ca1-xPrxMnO3 (0 ≤ x ≤ 0. 3) powders were prepared by the sol-gel auto-combustion route at different calcination temperatures and the effects of calcination temperature on the structure and electrical conductivity of the powders were characterized. It was indicated that the resistivity of modified powders represented the typical V type variation of firstly decreased and then increased with the increasing of calcination temperatures, and the resistivity was the lowest when the calcination temperature was at 1 000 °C. The XRD analysis illustrated that with the increasing of calcination temperatures, the diffraction peaks of Ca1-xPrxMnO3 powders were moved to the small angles with the strengthening in intensity. The crystallography data calculated from XRD illustrated that the cell parameters which related to the resistivity of Ca1-xPrxMnO3 powders were affected by the calcination temperatures. When the calcination temperature was at 1 000 °C, the crystal distortion was most distinct. The SEM analysis illustrated that the higher calcination temperature led to the larger size of grains, and the suitable grain size was good at decreasing the resistivity of Ca1-xPrxMnO3 powders. Moreover, the quantivalency of Mn was the lowest when the Ca1-xPrxMnO3 powders were calcined at 1 000 X.. The grain boundary, grain size and the quantivalency of Mn together lead to the decreasing then increasing type variation of the resistivity of Ca1-xPrxMnO3 powders calcined at different temperatures. [ABSTRACT FROM AUTHOR]

A dynamic monitoring on the rainfall-runoff process and the surface runoff and sediment yield in a sloping farmland was conducted at a natural rainfall runoff plot in the watershed of Jianshan River, the first tributary of Fuxian Lake, Chengjiang, aimed to study the regulation effects of reverse-slope level terrace on the runoff and sediment yield in red soil sloping farmlands of Yunnan. The regulation rates of runoff and sediment yield by the reverse-slope level terrace were 49. 5% -87. 7% and 56. 7% -96. 1% , averagely 65. 3% and 80. 7% , respectively, showing that the regulation effects of reverse-slope level terrace on the runoff and sediment yield, especially the latter in sloping farmland were prominent. The variation coefficients of the test parameters for undisturbed sloping farmland and reverse-slope level terrace were in the order of sediment yield > runoff > rainfall. Comparing with undisturbed sloping farmland, reverse-slope level terrace had smaller surface runoff and smaller relative deviation degree of sediment yield, demonstrating its remarkable effect in controlling runoff and sediment yield in sloping farmland. [ABSTRACT FROM AUTHOR]

Proposes a new method of estimating Jacobian matrixes on-line for image-based robot visual servo systems. A vector is firstly formed from the elements of a Jacobian matrix, and the problem is converted into one of state-estimation. Particle filtering suitable for non-liner non-Gaussian systems is utilized to solve the Jacobian estimation problem. The proposed method and the one based on Kalman filtering are tested to track a moving target on a two-degree-of-freedom system with non-Gaussian noise. The results showed the effectiveness and the robustness of the proposed method. System calibrations can be avoided and no specification on system noises is needed. [ABSTRACT FROM AUTHOR]

Background and purpose: Colorectal cancer (CRC) is one of the major malignant tumors threatening human health worldwide, with long-term high incidence and mortality rate. Potassium channel modulatory factor 1 (KCMF1) is a member of the E3 ubiquitin ligase family. It binds to target proteins through the RING domain and participates in the regulation of a variety of biological processes in vivo. However, the function of KCMF1 in CRC remains unclear. This study aimed to investigate the expression level of E3 ubiquitin ligase KCMF1 in colorectal tumor, and to explore the effects of KCMF1 on the proliferation of CRC cells and its underlying molecular mechanism. Methods: The The Cancer Genome Atlas (TCGA) and Genotype-Tissue Expression (GTEx) databases were used to analyze the expression level of KCMF1 in CRC tissues and adjacent tissues and the association between the KCMF1 expression and the prognosis of CRC patients. Furthermore, immunohistochemical staining was performed to detect the protein level of KCMF1 in 90 paired human CRC tissues and adjacent non-tumor tissues. Lentiviral shRNA delivery system was employed to specifically target the KCMF1 gene (shKCMF1) in HCT116 and HCT15 CRC cell lines. The effects of KCMF1 knockdown on cell proliferation, apoptosis and cell cycle distribution were assessed by methyl thiazoyl terazolium (MTT) assay, colony formation assay, Western blot and flow cytometry. Changes in the transcriptional profile in HCT116 cells upon KCMF1 knockdown were identified by RNA sequencing (RNA-Seq), and the affected signaling pathways were evaluated by bioinformatics analysis. Real-time fluorescence quantitative polymerase chain reaction (RTFQ-PCR), Western blot, luciferase reporter assay and cell immunofluorescence assay were utilized to validate the alteration of the affected signaling pathway. Results: The TCGA and GTEx databases and IHC results showed that the mRNA and protein expression levels of KCMF1 in CRC tissues were significantly upregulated compared with adjacent tissues (P

Intelligent command and control has become a new style of modern warfare command, and the high dynamic, strong adversarial, and massive data battlefield environment has put forward higher requirements for command efficiency and task execution ability. The traditional human-computer interaction mode has low interaction efficiency and lacks a human-centered interaction design, which cannot fully utilize the effectiveness of underwater command and control. This article is based on intelligent human-machine interaction and principal component analysis to design an interaction module that can generate an adaptive interface for underwater command and control based on user and business needs. By building an underwater command and control intelligent simulation platform, it has been confirmed that adaptive intelligent human-machine interaction can simplify the command and control process, reduce interaction time, and improve combat efficiency, with strong application value.

Objective: To study the chemical constituents of Swertia binchuanensis., Methods: The constituents were separated and purifyed by conventional methods. Their structures were identified on the basis of spectral analysis such as 1H-NMR,13C-NMR., Results: Seven compounds were isolated and identified as 1, 2, 3, 4-tetrahydro-1, 4, 8-trihydroxy-6-methoxyxanhone（ Ⅰ）, erythrocentaurin（ Ⅱ）,1-O-β-D-glucopyranosyl-1, 2, 3, 4-tetrahydro-4, 8-dihydroxy-6-methoxyxanthone（ Ⅲ）, maslinic acid（ Ⅳ）,1-hydroxy-3, 7-dimethoxyxanthone（ Ⅴ）,1, 8-dihydroxy-3, 5-dimethoxyxanthone（ Ⅵ）,1, 7-dihydroxy-3, 4, 8-termethoxyxanthone（ Ⅶ）., Conclusion: All compounds were isolated from Swertia binchuanensis for the first time.

Genomic imprinting is an epigenetic process that distinguishes parental alleles and results in specific expression of paternal and maternal genes. Imprints are acquired in the process of gametogenesis when genome-wide epigenetic reprogramming occurs and are maintained during early embryonic development. Therefore, the recognition and maintenance of imprints are very important in genome-wide reprogramming. In this review, we summarize the progresses of imprints removal in primordial germ cells (PGCs), imprints acquisition in parental PGCs, and imprints maintenance during early embryonic development. We also discuss the functional mechanisms of epigenetic factors which protect imprinted genes from whole genome DNA methylation.

Objective: To investigate the role of nesprin-1 in mouse embryonic stem cells differentiation into cardiomyocyte., Methods: Hanging drop-suspension-adherence method was applied for the differentiation of mouse embryonic stem cells into cardiomyocytes under the inducing of salvia miltorrhiza and 5-azacytidine. Changes in nesprin-1 gene expression were detected by using Western blotting and immunofluorescent assay. RNA interference was used to reduce nesprin-1 protein levels to further investigate the importance of nesprin-1 in mouse embryonic stem cells differentiation, group I (target sequence AAAGCCAAGCACGCAACTA), group II (target sequence GGGAACCAACAGTGAGATT), group III (target sequence ACCAGGACATTGCGTACTA), and group IV (control group)., Results: The nesprin-1 isoform profile was altered in mouse embryonic stem cells differentiation. The rates of differentiation of the four groups were (17.78 +/- 1.92)%, (36.67 +/- 3.34)%, (44.42 +/- 5.08)%, (77.78 +/- 1.92)%; The rate of differentiation of group IV was higher than RNAi groups and the difference was significant (P < 0.05). In addition, compared with the control group, myosin in RNAi groups were dramatically reduced., Conclusion: Nesprin-1 played important roles in mouse embryonic stem cells differentiation into cardiomyocyte. Nesprin-1 isoforms might perform different functions in the process of mouse embryonic stem cells differentiation.

A dynamic monitoring on the rainfall-runoff process and the surface runoff and sediment yield in a sloping farmland was conducted at a natural rainfall runoff plot in the watershed of Jianshan River, the first tributary of Fuxian Lake, Chengjiang, aimed to study the regulation effects of reverse-slope level terrace on the runoff and sediment yield in red soil sloping farmlands of Yunnan. The regulation rates of runoff and sediment yield by the reverse-slope level terrace were 49.5%-87.7% and 56.7%-96.1%, averagely 65.3% and 80.7%, respectively, showing that the regulation effects of reverse-slope level terrace on the runoff and sediment yield, especially the latter in sloping farmland were prominent. The variation coefficients of the test parameters for undisturbed sloping farmland and reverse-slope level terrace were in the order of sediment yield > runoff > rainfall. Comparing with undisturbed sloping farmland, reverse-slope level terrace had smaller surface runoff and smaller relative deviation degree of sediment yield, demonstrating its remarkable effect in controlling runoff and sediment yield in sloping farmland.

Objective: To study the feasibility of using Narcotrend (NCT) in monitoring the anesthetic depth during endotracheal intubation in sevoflurane anesthesia., Methods: Thirty ASA I-II patients (aged 20-49 years) undergoing gynecologic surgery under general anesthesia with tracheal intubation were randomized into sevoflurane group (n=15) and sevoflurane plus rocuronium group (n=15). In the former group, anesthesia was induced with sevoflurane at the primary concentration of 8% till the final end expiratory concentration reaching 2 MAC(minimum alveolar concentration) for 3 min, followed then by tracheal intubation and further observation of the indicators for another 3 min. The patients in sevoflurane plus rocuronium group received identical anesthesia procedures except for the administration of intravenous injection of rocuronium (0.6 mg/kg) after the loss of eyelash reflex. The NCT, BIS and hemodynamics were recorded during the process., Results: No significant differences were noted in NCT, bispectral index (BIS), MAP and heart rate before tracheal intubation between the two groups (P>0.05). The NCT and BIS increased significantly after tracheal intubation in sevoflurane group (P<0.05), but remained below 60. No significant changes in NCT and BIS occurred during intubation in sevoflurane plus rocuronium group (P>0.05). The mean arterial pressure (MAP) and heart rate were significantly increased in both groups after tracheal intubation in comparison with those before tracheal intubation (P<0.05), but the increment in sevoflurane group was significantly greater (P<0.05)., Conclusion: NCT may reflect the changes of the anesthetic depth resulting from the nociceptive stimulus of tracheal intubation in sevoflurane- induced anesthesia. NCT and BIS can not serve such a purpose in combined anesthesia with sevoflurane and rocuronium.

Objective: To study the correlation between the Narcotrend index, cerebral state index and predicted effect site concentration during different state of consciousness in the absence of surgery in elderly patients with target controlled infusion of propofol., Methods: Twenty patients aged from 65-75 years categorized as ASA class I - II who were scheduled to undergo general surgery under general anesthesia with target controlled infusion of propofol were recruited. During the target controlled infusion of propofol, the propofol infusion was set at an initial effect site concentration of 0.5 mg/L and increased by 0.5 mg /L every 5 min until the modified observer's assessment of alertness / sedation scale (OAA/S) values of zero. The predicted effect site concentration of propofol, the values of CSI and NCT were recorded and the sedation level was examined by the modified OAA/S every 20 s. The predicted effect site concentrations of propofol in target controlled infusion (TCI) system were recorded when they increased by more than 0.1 mg/L. The predicted effect site concentrations of propofol and the values of NCT and CSI at LVC and LOC of the patients were recorded., Results: There was a good linear correlation between NCT and the predicted effect site concentration of propofol (R2 = 0. 867, P < 0.01), as well as that between CSI and the predicted effect site concentration of propofol (R2 = 0.893, P < 0.01). The predicted effect site concentrations of propofol at LVC was (1.56 +/- 0.13) mg/L while the values of NCT was 74.00 +/- 4.69 and CSI 69.82 +/- 5.47. The predicted effect site concentrations of propofol at LOC was (2.15 +/- 0.27) mg/L while the values of NCT and CSI were 63.30 +/- 7.50 and 58.78 +/- 6.90 respectively. All of the values of NCT, CSI and the predicted effect site concentrations had a good linear correlation with OAA/S. There was a negative correlation between OAA/S and the predicted effect site concentration. At the same time, there was a positive correlation between OAA/S and NCT as well as that between OAA/S and CSI. And the correlation coefficients were - 0.968, 0.938, 0.940 respectively (P < 0.01). The values of NCT were higher significantly than that of CSI in different degree of LOC (P < 0.01)., Conclusion: During elder people's target controlled infusion of propofol, LVC and LOC occur within a definite range of predicted effect site concentrations. There is a good linear correlation between NCT, CSI and the predicted effect site concentrations of propofol. For the elders, both NCT and CSI reflect the sedation level of propofol. Although there is a significant correlation between NCT and CSI, a deviation does exist in a certain range. Therefore a simple 1:1 transfer from NCT to CSI is inadequate.

In order to construct an eukaryotic expression vector for gene of multiple myeloma mucin1 (muc1-2vntr) gene and to express it in COS-7 cells in vitro, so to provide the basic material for further research of multiple myeloma DNA vaccine. muc1-2vntr coding gene was used as a research gene and a KOZAK sequence was inserted before the gene Hind III and XbaI restriction sites were inserted before and after the coding gene. Then the whole sequence was synthesized and inserted into pcDNA3.1/myc-his B vector, and the resulted recombinant vector was transformed into E.coil competent cells to get an engineering strain, the recombinant plasmid pcDNA3.1-2vntr/myc-his B identified by restriction analysis and DNA sequencing were transfected into COS-7 cells by liposome-mediated gene transfer method. Finally, fluorescent microscopy was used to assess GFP expression and Western blot analysis using muc1 monoclonal antibody was used to recognize vntr, confirming the expression of vntr. The results showed that the full length of synthesized muc1-2vntr gene, as expected, was 140 bp. Both restriction analysis and DNA sequencing demonstrated that pcDNA3.1-2vntr/myc-his B included the whole translation frame region and muc1-2vntr gene. Furthermore, the fluorescence microscopy proved that the recombinant plasmid had been successfully transfected into COS-7 cells. The expression of mucin-1 protein was observed both in the transfected cell and the cell supernatant by Western blot. It is concluded that the pcDNA3.1-2vntr/myc-his B has been successfully constructed and expressed in COS-7 cells in vitro, which provides the basic material for further researches of mucin-1 function and possible multiple myloma DNA vaccine.

One DRV strain was isolated from Sika Deer brain and sequenced. Nine overlapped gene fragments were amplified by RT-PCR through 3'-RACE and 5'-RACE method, and the complete DRV genome sequence was assembled. The length of the complete genome is 11863bp. The DRV genome organization was similar to other rabies viruses which were composed of five genes and the initiation sites and termination sites were highly conservative. There were mutated amino acids in important antigen sites of nucleoprotein and glycoprotein. The nucleotide and amino acid homologies of gene N, P, M, G, L in strains with completed genomie sequencing were compared. Compared with N gene sequence of other typical rabies viruses, a phylogenetic tree was established . These results indicated that DRV belonged to gene type 1. The highest homology compared with Chinese vaccine strain 3aG was 94%, and the lowest was 71% compared with WCBV. These findings provided theoretical reference for further research in rabies virus.

Objective: To investigate the effects of intravenous anesthetics on LPS-induced inflammatory responses of primary cultures of rat glial cells in vitro., Methods: The primary cultures of rat glial cells were stimulated with lipopolysaccharide( LPS) to produce inflammatory responses. Glial cells were divided into 8 groups (n=4): blank control (Group C), LPS(Group L), 100micromol/L ketamine with LPS(Group K1), 1000micromol/L ketamine with LPS (Group K2), 30micromol/L propofol with LPS (Group P1), 300micromol/L propofol with LPS (Group P2), 3micromol/L midazolane with LPS (Group M1), and 30micromol/L midazolane with LPS (Group M2). TNF-alpha released into the culture media was measured by radioimmunity assay., Results: Compared with the blank control Group C, LPS-induced TNF-alpha productions in Group L, K1, K2, P1, P2, M1 and M2 increased significantly. The levels of TNF-alpha in Group K1 and K2 were significantly lower than those in Group L (P<0.05), but TNF-alpha productions in Group P1, P2, M1 and M2 were not significantly different as compared with that in Group L., Conclusion: Ketamine can reduce LPS-induced TNF-alpha production of glial cells, thereby inhabiting some of the inflammatory responses. Propofol and midazolam have no effect on the production of TNF-alpha from LPS-stimulated glial cells.

Objective: To study the effects of selective head mild hypothermia on endogenous neuroprotection in brain following global cerebral ischemia/reperfusion., Methods: Fifteen dogs were randomly divided into three groups: nonischemic control group (Group A, n = 4), undergoing thoracotomy without cerebral ischemia and general care for 8 hours thereafter; cerebral ischemia/reperfusion group (Group B, n = 5) undergoing thoracotomy, clipping of the ascending aorta for 18 min, cardiac resuscitation, maintenance of respiration and circulation for 8 h; and mild hypothermia group (Group C, n = 6), received selective head mild hypothermia, i.e. lowering the tympanic temperature to (34 +/- 0.5) degrees C for 8 hours after cerebral ischemia. The neurological function was assessed by Glasgow coma scale and Pittsburgh brain stem score. At the end of experiment, the dog brains were taken out to obtain the right parietal cerebral cortex. Immunohistochemistry was used to detect the parvalbumin (PV) and HSP(70). Xanthine oxidase method was used to detect the superoxide dismutase (SOD) activities: total SOD (T-SOD), manganese SOD (Mn-SOD), and copper-zinc SOD (Cu-ZnSOD). Spectrophotometry was used to detect the activities of glutathione (GSH) and glutathione peroxidase (GSH-Px)., Results: The comprehensive neurological score of Group C was 23.4 +/- 1.5, significantly higher than that of Group B (18.6 +/- 1.0, P < 0.05). The cerebral cortex of Group A showed a lot of PV positive neurons, the density of PV-positive neurons decreased significantly in Group B (P < 0.05), and the density of PV-positive neurons win Group C was significantly higher then that of Group B, however, still significantly lower than that of Group A (both P < 0.05). The density of HSP70-LI neurons of Group A was very low (5.5 +/- 2.1), those of Groups B and C were significantly higher than that of Group A (15.6 +/- 3.7 and 27.1 +/- 4.9 respectively, P < 0.05 or P < 0.01), that of Group C being significantly higher than that of Group B (P < 0.05). The contents of GSH, T-SOD, MnSOD, Cu-ZnSOD, and GSH-Px of Group B were all significantly lower than those of Group A (P < 0.05 or P < 0.01). The contents of GSH, T-SOD, and Cu-ZnSOD of Group C were significantly higher than those of Group B (P < 0.05 or P < 0.01),, Conclusion: Mild hypothermia may up-regulate the endogenous neuroprotection in brain tissue following cerebral ischemia/reperfusion and may be beneficial to cerebral ischemia.

Effect of pentachlorophenol (PCP) on COD and ammonium removal rates was investigated, and also changes of bacterial community within aerobic granular sludge were monitored by terminal restriction fragment length polymorphism (T-RFLP) technique. Compared with the removal rate of COD, the rate of ammonium removal is more sensitive to PCP. As the ammonium and COD removal rates decreased consistently, the bacterial genera within the aerobic granular sludge also decrease with the increase of PCP concentration. According to the response of PCP, the bacteria could be categorized into four groups as follows: (1) The most sensitive organisms such as Microbacterium, Streptococcus and two new kinds of microorganisms; (2) The more sensitive microorganisms such as Corynebacterium, Nevskia. and another unreported bacterium; (3) The lower sensitive organisms encompassing Mycoplasma, Exiguobacterium, and another microorganism which has not been reported yet; (4) The PCP-tolerant organisms, including microorganisms indicated by 13 TRFs are represented as 69, 71, 82, 175, 198, 241, 229, 232, 233, 240, 245, 269 and 449bp. To most noticeable, TRF relative areas of the microorganisms indicated by TRFs 82 bp and 175 bp achieve 22.7% and 13%, respectively, and become predominant within the aerobic granular sludge with the PCP concentration at 30 mg/L.

Objective: To observe the prophylactic effect of granisetron on postoperative nausea and vomiting (PONV) after supratentorial craniotomy., Methods: Seventy ASA I-II patients undergoing elective supratentorial craniotomy were randomly and double-bindly divided into two groups: control group (Group C, n = 35) and granisetron group (Group G, n = 35). All the patients received either 0.9% NS (Group C) or granisetron 3 mg (Group G) via intravenous injection. The incidences of postoperative nausea and vomiting were recorded at 24 h, 48 h, 72 h after supratentorial craniotomy., Results: The incidence of PONV in Group G was lower than that in Group C (25.7% vs 57.1%, P < 0.01). The incidence of server nausea with vomiting or vomiting in Group G was lower than that in Group C (17.2% vs 48.6%, P < 0.01)., Conclusion: Granisetron can be used effectively and safely to prevent PONV in supratentorial craniotomy.