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Environmental conditions regulate the growth and reproduction of fish. The increase in sea temperature during winter may have adverse effects on Takifugu rubripes. To study the mechanism of low-temperature tolerance of T. rubripes, the expression of antifreeze protein (AFP) gene, cold-induced RNA binding protein (CIRP) gene, high mobility group protein box-1 (HMGB1) gene, and Y-box binding protein (YB-1) gene in the liver, spleen, kidney, brain, heart, intestine, muscle, gonad, and skin tissues of T. rubripes obtained from different temperatures (18℃, 13℃, 8℃, and 5℃) was analyzed by quantitative real-time PCR. The results showed that the AFP gene was widely expressed in tissues, with the highest expression in the muscle (P < 0.05). With the decrease in temperature, the expression of the AFP gene in each tissue showed a significant increasing trend, reaching the highest value in the 5℃ group. The expression of the CIRP gene was the highest in the muscle (P < 0.05). With a decrease in temperature, the trend of CIRP gene expression in various tissues was different. The CIRP gene expression levels of liver, kidney, brain, heart, intestine, and skin showed a trend of initial increase, followed by a decrease, and then an increase. The expression levels in the spleen, muscle, and gonads showed an upward trend, reaching the highest value in the 5℃ group. The expression of the HMGB1 gene was the highest in muscle (P < 0.05), followed by that in the brain, liver, heart and skin. As the temperature decreased, the expression of the HMGB1 gene in all tissues except the liver increased first and then decreased, and reached the maximum value in the 8℃ group, which was significantly higher than that of the other groups (P < 0.05). The expression of the YB-1 gene was the highest in the muscle (P < 0.05), with the lowest expression level in other tissues. As the temperature decreased, the expression level of most tissues (brain, heart, intestine, kidney, liver, muscle, and spleen) increased first, then decreased, and then increased, reaching the minimum value in the 8℃ group (P < 0.05). These results show that the expression levels of the four genes are different at different temperature, reflecting the functional specificity of these four genes. Under low-temperature stress, these genes responded positively. Their expression changed to varying degrees, suggesting that the four genes may have potentially important roles in the adaptation of T. rubripes to low temperatures. In addition, by analyzing the law of gene expression, 8℃ may be the key regulatory point for T. rubripes to deal with low-temperature stress. Too low temperature may cause its regulation disorder. The results of this study can provide a relevant basis for studying the regulation mechanism of the low-temperature response of T. rubripes.

Y-box结合蛋白（YB-1）是冷休克蛋白家族的一员，参与目的基因的转录和翻译调节等多种细胞过程。本文就YB-1的细胞生理作用做一综述。 [ABSTRACT FROM AUTHOR]

Objective To study the expression of YB-1 in advanced cervical cancer tissues and the relationship between its expression and the clinicopathological characteristics, to investigate the role of YB-1 in cervical cancer invasion, metastasis and progress. Methods Immunohistochemistry was used to detect the expression of the YB-1 in 78 cases of middle and advanced stage cervical cancer tissue microarray chip and the relationship between its expression and the lymphatic metastasis, FIGO stage and the differentiated degree were analyzed. Results (1) The positive expression of YB-1 in cervical cancer was 24/78(30.8%); (2) The expression of YB-1 in cervical cancer was significantly related to FIGO stage and differentiation degree respectively. With the increase of FIGO stage and the decrease of differentiation degree, the expression of YB-1 increased gradually( P<0.05); The expression of YB-1 in cervical cancer was not significantly related to the lymph node metastasis. There was no significant difference between the lymph node metastasis positive and negative group(P > 0.05). Conclusion The expression of YB-1 may be closely related to the local invasion, metastasis and progress of cervical cancer. [ABSTRACT FROM AUTHOR]

Objective: To investigate the expression of FOXC-2,YB-1 and related proteins and their influences on development,invasion and metastases in gastric carcinoma., Methods: A total of 193 tissue samples were collected,including 50 cases of normal gastric mucosa, 50 cases of gastric mucosal intraepithelial neoplasia and 93 cases of primary gastric carcinoma. The 93 cases of primary gastric carcinoma included 74 cases of positive lymph node metastasis tissues, 19 cases of nonmetastasis tissues and 33 cases of distant metastasis tissues. Immuohistochemistry was used to detect the expression and distribution of FOXC-2,YB-1,E-cadherin, Vimentin and MMP-2 in normal and intraepithelial neoplasia,gastric carcinoma,positive lymph node metastasis tissues and distant metastasis tissues., Results: The expressions of FOXC-2,YB-1,Vimentin and MMP-2 in gastric carcinoma were significantly higher than those in normal and intraepithelial neoplasia while the expression of E-cadherin was significantly lower ( P <0.05).The expressions of FOXC-2,YB-1 were significantly correlated with low expression of E-cadherin and high expression of Vimentin and MMP-2 ( P <0.05). The expression of FOXC-2 protein was significantly correlated with TNM stage,lymph node metastasis and distant metastases ( P <0.05).The expression of YB-1 protein was significantly correlated with TNM stage,differentiation degree,invasion depth,lymph node metastasis and distant metastasis ( P <0.05). The expression of MMP-2 protein was closely related to the degree of differentiation,invasion depth,lymph node metastasis and distant metastasis ( P <0.05)., Conclusion: FOXC-2, YB-1 may be related to the occurrence, development, invasion and metastasis of gastric carcinoma. The possible mechanism is to promote the invasion and metastasis of cancer cells by activating the epithelial mesenchymal transition process and up regulating the expression of MMP-2., (Copyright© by Editorial Board of Journal of Sichuan University (Medical Science Edition).)