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To survey the virulence gene carrying status, drug sensitivity, and genetic characteristics of Vibrio parahaemolyticus, 26 seafood samples from three canteens in the Beidaihe area, as well as self-collected samples, were analyzed in August 2021. Four major virulence genes (tdh, trh, tlh, and toxR) were screened from ten strains of V. parahaemolyticus using PCR and the BD automatic microbial identification and drug susceptibility analysis system. Next-generation genome sequencing was performed on the isolates. A phylogenetic tree, drug resistance genes, and virulence genes were constructed based on core SNP sites. The gene carrying rates of tlh, toxR, were 100.00%, 100.00%; none of the isolates carried tdh or trh. Ten isolates were resistant to aztreonam and moderately sensitive to ampicillin. All the isolates contained the β-lactam resistance gene (CARB-18), corresponding to the resistance phenotype. One isolate was found to contain mexE, a virulence gene rarely reported in similar studies. Nine sequence types were identified. Several V . parahaemolyticus isolates exhibited high homology with local and foreign marine organism isolates. Local V. parahaemolyticus may pose a threat to human health, and its sources are complex and highly genetically diverse. There may be foreign sources of V. parahaemolyticus contamination; hence,monitoring needs to be strengthened. [ABSTRACT FROM AUTHOR]