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In order to accurately determine the level of fatigue of coal mine monitoring and dispatching operators and to reduce the error rate of coal mine monitoring and dispatching operations, a simulation experiment of coal mine monitoring and dispatching was carried out using eye tracking technology to collect the operator’s eye movement data and objective and subjective fatigue determination values. The K-means clustering algorithm was used to classify fatigue levels and train a neural network to build a fatigue prediction model for coal mine monitoring and dispatching operations. The results showed that the best fatigue level was classified into three categories and the fitting degree of the neural network prediction model was 90.58%. The prediction model was tested on a coal mine monitoring operation model in Shanxi Province, and the average error of the model field prediction was 6.26%, which was a good prediction effect.

Objective To observe the efficacy and side effects of olanzapine in the treatment of nausea and vomiting caused by opioid analgesics in cancer patients. Methods We randomly divided 85 cancer patients with nausea and vomiting caused by opioid analgesics into two groups. Treatment group was treated with olanzapine tablets and control group received metoclopramide injection solution. The clinical effect and medical side effects were compared between two groups. Results Compared with control group, the treatment group was significantly better in inhibiting nausea, vomiting and reducing opioid discontinuation rate (P < 0.05). Two groups were similar in drowsiness, dizziness, edema and other adverse reactions rate (P > 0.05). The control group showed significantly higher possibility in extrapyramidal reaction (akathisia) than the treatment group (P=0.024), while only one case of tumble happened in treatment group. Conclusion Olanzapine is a good choice in the treatment of nausea and vomiting caused by opioid analgesics and worth extending its clinical application.

To achieve the goals of carbon emission-peak and carbon neutrality, the energy conservation problem of subway air-conditioning system has become more and more prominent. Since the load of the subway equipment management room varies obviously with the start and stop of the equipment, the traditional air-conditioning system of constant air volume cannot change the air supply volume of the system, which would cause the waste of the cooling capacity. The air-conditioning system of variable air volume can be used to achieve the air supply on demand for different rooms at different times. This paper builds a simulation platform of the VAV air-conditioning system for a subway station in Zhengzhou and the platform is integrated with the load model of the subway equipment room to achieve on-demand air supply. The simulation platform is used to simulate the control characteristics and air volume characteristics of the VAV system. By adjusting the valve opening of the VAV-box terminal, the demanded air volume of the room is supplied on according to different load demands. The results show that the actual operating air volume basically matches the demand air volume and the deviation is less than 1%. The control effects of room temperature between the constant air volume system and the VAV system are compared. The room temperature with the constant air volume system is decreased to 34 °C, which is lower than the design temperature of 36 °. While, the room temperature with the VAV system can be precisely controlled at 36 °, and the waste of cooling capacity is avoided. [ABSTRACT FROM AUTHOR]

The intelligent phone thermal imaging instrument is applied in the chemistry experiment teaching. FLIR One Pro thermal imager can directly observe the thermal movement and thermal phenomena of some physical and chemical processes such as crystallization, evaporation and smoldering of metal particles, etc. The experimental results have proved that the performance of this kind of instrument can meet the needs of chemical experiment teaching, and students can directly observe the naked eye phenomenon by a mobile phone. [ABSTRACT FROM AUTHOR]

The quality of experimental teaching is closely related to the talent training. Supervision is one of great importance parts in the quality monitoring system of experimental teaching. This paper analyzed the necessity of establishment of experimental teaching supervision, introduced some experience of construction of supervision in Tongji University in recent years from these aspects-organization and implementation of experimental teaching supervision, management and monitoring of experimental teaching process, the experimental teaching evaluation. Showing some true cases, it also described the function of supervision in quality monitoring system of experimental teaching and summarized the effect of supervision, and gave specific views and perspectives of the development and future of the work. [ABSTRACT FROM AUTHOR]

Objective: To investigate the association between-1304T/G polymorphism in the promoter of MKK4 gene and the susceptibility in sporadic nasopharyngeal carcinoma., Method: MKK4-1304T/G genotypes were determined by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) in 90 NPC cases and 30 healthy controls., Result: The number of nasopharyngeal carcinoma patients carrying with TG+GG genotype was much higher than those of controls (82.2% vs 66.7%, χ² =10.076, P < 0.05). Analysis showed that compared with the-1304TT genotype, -1304TG heterozygous reduced risk of nasopharyngeal carcinoma 0.56 fold (95% CI = 0.164-1.178, P < 0.01) and-1304GG lower 0.58 fold (95% CI = 0.126-1.381, P < 0.01), TG+ GG genotype variation risk of nasopharyngeal carcinoma decreased 0.72 fold (95% CI = 0.105-0.753, P < 0.01)., Conclusion: MKK4 gene-1304TG genotype can reduce risk of nasopharyngeal carcinoma, and it may be an independent protection factor in sporadic nasopharyngeal carcinoma.

Objective: To investigate the effect of gene silencing of Bmi-1 on proliferation regulation of CD44+ nasopharyngeal carcinoma cancer stem-like cells (CSC-LCs)., Method: The sequence-specific short hairpin RNA lentivirus targeting at human Bmi-1 gene (LV-Bmi-1shRNA) was constructed and was used to infect CD44+ nasopharyngeal carcinoma cells which were sorted by flow cytometry. A lentiviral which included a random sequence was also designed to serve as a negative control. We employed fluorescence microscope and flow cytometry to detect infection efficiency; real-time PCR was used to detect Bmi-1 and its downstream gene while each protein expression level was confirmed by western blotting protocol; CCK-8 proliferation assay was applied to measure proliferation capacity; tumor spheroid assay was used to evaluate the self-renewal capacity. Colony formation assay was used to measure cell colony formation capability; flow cytometry analyzed cell cycle distribution., Result: The constructed LV-Bmi-1shRNA successfully infected into the CD44+ nasopharyngeal carcinoma cells. The infection efficiency could reach above 95%; LV-Bmi-lshRNA effectively inhibited Bmi-1 mRNA and protein expression, while the downstream gene p16INK4a and p14ARF mRNA as well as protein expression level were upregulated (P < 0.05). Notablely, the proliferation, colony formation, self-renewal capabilities of the experimental group decreased significantly (P < 0.05). In addition, the cell cycle arrested at the G0-G1 phase., Conclusion: Gene silencing of Bmi-1 inhibited the proliferation, colony formation and self-renewal capabilities of the CD44+ nasopharyngeal carcinoma CSC-LCs, inhibited the cell cycle processes, which may mediate through Bmi1-p16INK4a/p14ARF-p53 pathway. Our experimental results indicated that Bmi-1 gene may play an important role in the maintenance of the stem cell-like characteristics of CD44+ nasopharyngeal carcinoma cells. Bmi-1 gene may be a potential new target for the treatment of nasopharyng al carcinoma in the future.

Objective: To discuss the preoperative, intraoperative, and postoperative application of echocardiography in mini-invasive surgical device closure of secundum atrial septal defects, including those special and difficulty-occluded defects., Methods: We performed mini-invasive surgical device closure of secundum atrial septal defects on 287 patients. Before the surgery, transthoracic echocardiography was applied for screening; during the surgery we reassessed the sizes of the defects and their remaining margins, designated the suitable occluders, and guided the placement of the occluders by multiplane transesophageal echocardiography. The patients were postoperatively followed up at regular intervals by multiplane transesophageal echocardiography (MTEE) which was employed to assess the therapeutic efficacy., Results: Out of the 287 atrial septal defects, 276 (96.17%) were successfully closed. There were 37 porous defects and 23 cases with short posterior-inferior margin of defects. Follow-ups at intervals showed the occluders stayed firmly and echoed clearly. No notable residual shunt or valve regurgitation was observed., Conclusion: Echocardiography plays a vital and reliable role in mini-invasive surgical device closure of secundum atrial septal defects, especially those special and difficulty-occluded defects.

Objective: To discuss the suitable types of ventricular septal defects for asymmetric occluders, and elucidate the critical role of echocardiography in choosing occluders, guiding successful occlusion and avoiding injury during operation., Methods: We retrospectively studied 179 patients with ventricular septal defects who received minimal-invasive surgical device closure with asymmetric occluder. We analyzed the types, size and morphology of ventricular septal defects suitable for asymmetric occluders. The therapeutic efficiency was evaluated by follow-ups., Results: Out of the 179 successful cases treated with asymmetric occluders, 86.59% had perimembranous ventricular septal defects, and double-committed sub-arterial ventricular septal defects accounted for 13.41%. In general, the size of occluders to be selected was the maximum diameter of the defects plus 2-3 mm. Follow-ups showed that occluders were placed and fixed properly. No severe residual shunt, valve regurgitation or heart block were discovered., Conclusion: Application of asymmetric occluders expands the range of indications for occlusion via small chest incision. Accurate echocardiography helps to improve the safety and successful rate of the surgery.

Objective: To elucidate the preoperative, intraoperative, and postoperative utility of echocardiographic technology in occlusion of ventricular septal defect (VSD) via small chest incision., Methods: We performed occlusion of VSD via small chest incision in 446 children. Before surgery, a multiple-section transthoracic echo-cardiogram (TTE) was employed to evaluate various parameters (including the size, position, and type) of the VSD region as well as the condition of neighboring tissues. During surgery we reassured the size of the occluder, meanwhile, guiding placement of the occluder by transesophageal echocardiogram (TEE). Patients also received postoperative follow-ups at regular intervals., Results: Out of the 446 VSD cases, the defects of 412 patients were successfully closed; a 92.4% successful rate. All patients received follow-ups one year after operation. The occluders had stayed firmly and echoed clearly. No notable residual shunt or valve regurgitation was discovered., Conclusion: During occlusion of VSD via small chest incision, echo-cardiogram technology (including TTE and TEE) can play a vital role by helping screen cases preoperatively, by guiding the closure intraoperatively, and by evaluating therapeutic efficacy postoperatively, providing relatively accurate and accountable results at all stages.

Objective: To introduce peratrial device closure of secundum atrial septal defects (ASD) under the guidance of transesophageal echocardiography (TEE) without cardiopulmonary bypass (CPB) in children, and to summarize the clinical experiences., Methods: A total of 115 children with secundum ASD (the occlusion group) underwent peratrial device closure of atrial septal defects through a small sternotomy under TEE guidance without cardiopulmonary bypass. Children were followed up closely for 1-13 months. Another 59 children (the bypass group) had closed atrial septal defects under cardiopulmonary bypass during the same period. The differences in the operation duration, convalescence and complication between the 2 groups were compared., Results: Except 1 patient was operated under the CPB, the rest 114 patients in the occlusion group were successfully closed by the occluders. The duration of the operation, mechanical ventilation, intensive care and hospitalization, and the rate of blood-transfusion in the occlusion group were significantly lower than those in the bypass group (P<0.01), with no difference in complications in the 2 groups (P>0.05)., Conclusion: Minimally invasive peratrial device closure of ASD without CPB is a relatively simple, safe and effective operation under the guidance of TEE for children. The short and mid-term clinical outcomes are promising. Long-term follow-up is indispensable.

Objective: To detect the effect of Celecoxib on the proliferation and apoptosis of human nasopharyngeal carcinoma cell line CNE-2., Method: The growth inhibition rate of CNE-2 by Celecoxib was evaluated with MTT method. Apoptosis related morphology changes were observed with transmission electron microscopy (TEM). The cell cycle and apoptosis were measured with flow cytometric method (FCM). Apoptotic index (AI) was counted by the TDT-mediated dUTP-biotin nick end-labeling (TUNEL) assay., Result: The growth of CNE-2 cell was inhibited by celecoxib in a dose-and time-dependent manner. Apoptosis with nuclear chromatin condensation, cell shrinkage, periplasm loss and the formation of apoptotic bodies was observed with TEM. Apoptotic rates of CNE-2 cells treated with 80 and 100 micromol/L celecoxib were (10.47+/-0.18)% and (20.17+/-0.55)% respectively, significantly higher than those of the control group (1.57+/-0.27)% with FCM. The percentage of G0/G1 phase cells increased, whereas the S and G2/M phases cells decreased in a dose-dependent manner after the treatment. TUNEL assay showed that the apoptosis ratio (AI) of CNE-2 treated with Celecoxib was higher than control group (P<0.01)., Conclusion: Celecoxib can inhibit the growth of human nasopharyngeal carcinoma cell line CNE-2 and induce the cell apoptosis, which may be related to blocking the cell cycle progress of CNE-2 cells.