1. [Action of apoptosis-induced ligand gene in relation to tumor necrosis factor on human colon cancer cell line HT29].
- Author
-
He C, Hu X, Xu X, and Fang B
- Subjects
- Adenoviridae genetics, Apoptosis genetics, Apoptosis Regulatory Proteins, Cell Division genetics, Cell Division physiology, Colonic Neoplasms genetics, Colonic Neoplasms therapy, Genetic Therapy methods, Genetic Vectors genetics, HT29 Cells, Humans, Membrane Glycoproteins genetics, TNF-Related Apoptosis-Inducing Ligand, Transfection, Tumor Necrosis Factor-alpha genetics, Apoptosis physiology, Colonic Neoplasms pathology, Membrane Glycoproteins physiology, Tumor Necrosis Factor-alpha physiology
- Abstract
Objective: To evaluate the gene therapeutic efficiency of apoptosis-inducing ligand (TRAIL) related to tumor necrosis factor on human colon cancer cell line HT29., Methods: Human colon cancer cell line HT29 was transfected with adenovirus-mediated TRAIL gene Ad/GT-TRAIL. The morphological changes, cell growth and apoptosis were measured by phase contrast microscope, MTT method and flow cytometry., Results: Obvious morphological changes in HT29 cells was induced by Ad/GT-TRAIL and Ad/PGK-GV16. The cell suppression percentage and the percentage of apoptotic cells were 54.3% and 11.1%, respectively. When used in combination with Ad/PGK-GV16, HT29 was suppressed to 82.7% and the percentage of apoptotic cells was 24.6%. This result showed significantly enhanced therapeutic efficiency on HT29 and thus inhibiting of its growth (P < 0.05)., Conclusion: Ad/GT-TRAIL is able to induce apoptosis of HT29 and inhibit its growth. Ad/GT-TRAIL shows significantly enhanced therapeutic efficiency for HT29 when used in combination with Ad/PGK-GV16.
- Published
- 2002