1. Correlation Analysis between the Active Ingredients and the Antioxidant or Cholate-binding Ability of Ethanol Extracts from Psoralea corylifolia L.
- Author
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Xinyang JIANG, Jinyue LIANG, Jitong SHI, Lanling LIU, Shanshan ZHANG, Pan ZHAO, Peizheng YAN, and Dongsheng ZHAO
- Subjects
psoralea corylifolia l. ,ethanol extracts ,antioxidant ,cholate ,correlation analysis ,hypolipidemic ,Food processing and manufacture ,TP368-456 - Abstract
Objective: The contents of main active ingredients, antioxidant and cholate binding ability of different ethanol extracts from Psoralea corylifolia L. were explored based on the mode of correlation of “components-efficacy”. Methods: Psoralea corylifolia L. was extracted with anhydrous ethanol, 75% ethanol, 50% ethanol and water. The contents of total flavonoids and total polyphenols in Psoralea corylifolia L. were determined. The antioxidant and lipid-lowering activities in vitro of Psoralea corylifolia L. were evaluated by three antioxidant systems and the binding ability of cholate salts, respectively, and the correlation analysis was conducted. Results: The contents of total flavonoids and total polyphenols in anhydrous ethanol extract were the highest, (50.317±0.018) mg/g and (3.860±0.045) mg/g, respectively. The 50% ethanol extract showed the best ability to scavenge ABTS+· and DPPH·, the IC50 values were 0.76 and 1.63 mg/mL, respectively. The anhydrous ethanol extract showed the strongest reducing ability, the A0.5 value was 2.63 mg/mL. The water extract showed the strongest binding capacity to sodium cholate, the IC50 value was 0.67 mg/mL. The 50% ethanol extract showed the strongest binding capacity to sodium taurocholate and sodium glycinate, the IC50 values were 1.26 and 0.41 mg/mL, respectively. The correlation results showed that the content of total flavonoids of different ethanol extracts from Psoralea corylifolia L. had a significant positive correlation with DPPH· clearance rate and sodium taurocholate binding rate (P
- Published
- 2022
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