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The construction of "zero-free cities" is an effective plan to achieve the carbon peak plan, reduce pollution and carbon emissions, and promote a circular economy. Based on the WARM model and Emission factor method, the total carbon emission reduction of solid waste sources and disposal in each field during the implementation of the zero-free city policy in Chongqing （2017-2021） was calculated, and the total carbon emission reduction of solid waste in each field in 2025 was predicted by scenario. The results showed that： ① After the implementation of cleaner production and green manufacturing policies in Chongqing, the generation intensity of general industrial solid waste decreased to 0.20 t·（10 4 yuan） -1 in 2021, and the carbon emission reduction in the industrial sector had been increasing since 2020. Due to the growth of the economy and living level, the per capita production of food waste, domestic waste, and e-waste increased, failing to reduce emissions at the source in these areas. In addition, the promotion of green food and organic agricultural products and the restriction of the use of chemical fertilizers and pesticides could be strengthened to further reduce source emissions in the agricultural field. ② General industrial solid waste, construction waste, and straw were the key areas to achieve carbon emission reduction, and their carbon emission reductions were predicted to increase to 2 796.64×10 4 , 538.54×10 4 , and 99.07×10 4 t in 2021. Respectively, reducing the landfill volume in the above fields is the key to emission reduction. In the field of daily life, the collection and transportation of solid waste should be improved, the sorting process and disposal facilities should be refined, and the resource recycling rate should be improved to reduce its carbon emissions. Due to the improvement of the collection level and the application of straw feed and fertilizer, the carbon emission reduction of straw and agricultural film is generally rising, and the proportion of straw and livestock manure directly returned to the field should be reduced in the agricultural field, and the allocation of biomass disposal facilities to promote the co-disposal of the two may be the best means of carbon emission reduction. ③ In 2025, the predicted carbon emission reductions in Chongqing under the BAU scenario and the PLAN scenario were 2 289.78×10 4 and 2 750.31×10 4 t, respectively, indicating that the upgrading and transformation of local industries and the large-scale scale of the renewable resources industry have achieved good carbon reduction benefits. With the construction and improvement of various waste disposal facilities in 2025, the carbon emission reduction of the solid waste disposal process in various fields will continue to increase, but the carbon emissions of hazardous waste, municipal sludge, and livestock and poultry manure will still be in a state of growth due to the possible increase in total output in the future.

Objective: To explore the differences between hematological phenotypes of patients with different genotypes in gene mutations and deletion α- thalassemia., Methods: By screening the α- thalassemia gene test results in the First Affiliated Hospital, Sun Yat-Sen University from January 2015 to April 2020, the patients with mutation and deletion α- thalassemia were obtained, then the differences between hematological phenotypes of patients with different genotypes were analyzed., Results: There were 96 patients with mutation combined with deletion α- thalassemia from the results of 24 054 α- thalassemia patients screened out, including 79 patients with non-deletion Hb H disease (α T α/-- SEA ) and 17 patients with mild α- thalassemia (α T α/-α), the incidence was 0.42%. Except the number of red blood cells (RBC) and mean corpuscular volume (MCV), the hemoglobin (Hb) concentration, hematocrit (Ht), average red blood cell hemoglobin concentration (MCHC), average red blood cell hemoglobin amount (MCH), average red blood cell volume (MCV) of the patients with α T α/--SEA genotype were significantly lower than those with α T α/-α genotype. The Hb of the patients with α CS α/-- SEA and α QS α/-- SEA genotype was (86±20)g/L and (84±9)g/L, respectirely, which was significantly lower than (114±16) g/L of α WS α/-- SEA genotype (P<0.05); The MCHC of patients with α CS α/-- SEA and α QS α/-- SEA genotype was (278.8±8.5) g/L and (282.1±21.1)g/L, respectirely, which was also significantly lower than (315.4±19.5) g/L of α WS α/-- SEA genotype (P<0.05); There was no significant difference between the patients with α CS α/-- SEA and α QS α/-- SEA genotype in hematological phenotypes. Except MCH and MCV, there was no significant differences between the patients with α WS α/-- SEA and α T α/-α genotype in RBC, Hb, and Ht. The result of Hb A 2 was (2.3±0.9)% for only 27 patients who performed electrophoretic analysis. There was no significant difference between the patients with α T α/-- SEA and α T α/-α genotype in Hb A 2 , aslo among 3 types of the patients with α T α/-- SEA genotype., Conclusion: The hematological phenotype changes caused by α WS α/-- SEA genotype are similar to those of mild α- thalassemia, and both of them are significantly lighter than those patients with α CS α/-- SEA and α QS α/-- SEA genotype.

Objective: To investigate the effect of the long chain non-coding RNA H19 (lncRNA H19) on the invasion and migration of oral cancer cells and its related molecular mechanism., Methods: The expression levels of lncRNA H19, miR-107, and cyclin-dependent kinase 6 (CDK6) in the immortalized oral epithelial cell line HIOEC and the oral cancer cell line CAL27 were detected by real-time quantitative polymerase chain reaction. CAL27 cells were transfected with siRNA H19, miR-107 mimics, pcDNA H19, or anti-miR-107, and the effects of H19 and miR-107 on the invasion and migration of cells were examined via Transwell assay. The TargetScan database predicted the targeting of H19, miR-107, and CDK6. Double luciferase reporter gene assay was performed to detect interactions among H19, miR-107, and CDK6. Western blot analysis was conducted to examine the effects of H19 and miR-107 on the protein level of the target gene CDK6., Results: Compared with that in HIOEC cells, the expression of H19 was significantly increased in CAL27 cells (P<0.05). After transfection with siRNA H19, the expression of H19 decreased, and the invasion and migration ability of CAL27 cells were inhibited (P<0.05). H19 could bind specifically to the 3'-UTR of miR-107 to modulate the expression of miR-107. Compared with that in HIOEC cells, the expression of miR-107 significantly decreased in CAL27 cells (P<0.05). The expression of miR-107 increased after transfection with siRNA H19, and anti-mir-107 co-transfection could promote the invasion and migration ability of siRNA H19 in CAL27 cells (P<0.05). Compared with that in HIOEC cells, CDK6 expression significantly increased in CAL27 cells (P<0.05), and the expression level of the gene was coregulated by H19 and miR-107 (P<0.05)., Conclusions: lncRNA H19 plays an important role in the development of oral cancer. It can regulate the invasion and migration of oral cancer cells by targeting the miR-107/CDK6 signaling axis.

A high-content GABA was found in Sojae Semen Praeparatum(SSP), which is a famous traditional Chinese medicine and officially listed in Chinese Pharmacopoeia. To screen out and identify GABA-producing microbes from samples at different time points during the fermenting process of SSP, traditional microbiological methods combined with molecular biological methods were used to study the predominant GABA-producing microorganisms existing in the fermenting process of SSP. This study would lay a foundation for further studying the processing mechanism of SSP. The fermenting process of SSP was based on Chinese Pharmacopoeia(2010 edition), and samples were taken at different time points during the fermenting process of SSP. The bacteria and fungi from samples at different time points in the fermenting process of SSP were cultured, isolated and purified by selective medium, and dominant strains were selected. The dominant bacteria were cultured in the designated liquid medium to prepare the fermentation broths, and GABA in the fermentation broth was qualitatively screened out by thin-layer chromatography. The microbial fermentation broth with GABA spots in the primary screening was quantitatively detected by online pre-column derivatization and high performance liquid chromatography established in our laboratory. GABA-producing microorganisms were screened out from predominant strains, and their GABA contents in fermentation broth were determined. The DNA sequences of GABA-producing bacteria and fungi were amplified using 16S rDNA and 18S rDNA sequences by PCR respectively. The amplified products were sequenced, and the sequencing results were identified through NCBI homology comparison. Molecular biological identification was made by phylogenetic tree constructed by MEGA 7.0 software. Through the homology comparison of NCBI and the construction of phylogenetic tree by MEGA 7.0 software, nine GABA-producing microorganisms were screened out and identified in this study. They were Bacillus subtilis, Enterococcus faecium, E. avium, Aspergillus tamarii, A. flavus, A. niger, Cladosporium tenuissimum, Penicillium citrinum and Phanerochaete sordida respectively. For the first time, nine GABA-producing microorganisms were screened out and identified in the samples at different time points during the fermenting process of SSP in this study. The results indicated that multiple predominant GABA-producing microorganisms exist in the fermenting process of SSP and may play an important role in the formation of GABA.

Objective: To investigate the drug distribution in tissues of cervical lymph node metastasis mice model after submucosa adjacent cancer injection of pingyangmycin-activated carbon nanoparticles (PYM-CH-NP) and evaluate the lymph targeting effect of PYM-CH-NP., Methods: Pingyangmycin (PYM) was radiolabeled with 125I by modified the chloramine T method. Cervical lymph node metastasis mice model was established by buccal submucosa inoculation of a high lymph metastasis cell line U14 cancer cell. 360 mice models burdened with cervical lymph metastasis were randomly divided into 3 groups. PYM group was treated with PYM water solution, PYM-CH-NP group was treated with PYM-CH-NP. Negative control group was injected with activated carbon nanoparticles. PYM-CH-NP and pingyangmycin water solution were injected in pericancer submucosa of the mice respectively. The radioactivity of drug in blood, heart, liver, spleen, lung, kidney and cervical lymph node were measured after 0.5, 1, 4, 8, 12, 24, 48, 72, 96, 120, 144, 168 h administration. The radioactivity of each samples per unit weight were calculated. The selectivity index (SI) and targeting index (TI) of drug were calculated., Results: The radioactivity of drug in cervical lymph node of PYM-CH-NP group was much higher than PYM group in each time point (P < 0.001), whereas the blood, heart, liver, spleen, lung and kidney uptake of pingyangmycin was greatly decreased in PYM-CH-NP group after 4 h administration (P < 0.001). The SI value of PYM group at each time point was less than 1. While the minimum SI and TI value of PYM-CH-NP was 1.793 and 1.562, the maximum value reached to 68.126 and 14.623 after 72 h administration., Conclusion: PYM-CH-NP can increase drug dosage in metastasized cervical lymph nodes, and decrease drug dosage of other organs. So better therapeutic outcome and little adverse reaction may be achieved for lymph node metastasis.

Objective: To study the allocation of embedded teeth in jaws using the three-dimensional reconstruction technique of 64-slices spiral CT., Methods: 27 cases were examined by helical scanning of axial view volume scan CT. The exact localization of the embedded teeth in jaws was acquired by using volume rendering (VR), maximum intensity projection (MIP), multiplanar reformation (MPR) and curve plane reconstruction (CPR)., Results: The localization, morphous, size, erupted orientation and relationship between surrounding tissues of the 41 embedded teeth in 27 patients were displayed by effectually using the images of VR, MIP, MPR and CPR. The election of orthodontic treatment or surgical intervention was decided by using 64-slices spiral CT., Conclusion: The exact data and objective evidence of the treatment plan could be provided by 64-slices spiral CT which will have important clinical application.

This paper reported a 16-year-old girl suffering from painless swelling in the right submandibular region which was diagnosed as ranula, but during the surgery, it was seen that the cyst was closely related to the submandibular gland and it was proved by pathological diagnosis as submandibular cyst. Submandibular cyst and ranula should be distinguished clinically, in order to avoid misdiagnosis and mistreatment.

Objective: To investigate the effect of the traditional Chinese medicine Matrine on cell cycle and human telomerase reverse transcriptase (hTERT) of human ACC-M cell lines., Methods: Different concentrations of Matrine were used in the medium of ACC-M cells. Change of cell cycle were detected by flow cytometry after ACC-M cell were cultivated with different concentrations Matrine (0.25, 0.50, 0.75, 1.00 mg x mL(-1)). Expression of hTERT was investigated by reverse transcription-polymerase chain reaction (RT-PCR) and indirect immunofluorescene and flow cytometry quantitative analysis., Results: Matrine caused obviously the GdG1 phase block and inhibited proliferation of ACC-M cells. At same time, this effect was positive correlation to Matrine concentration and treat time. Matrine can inhibit the expression of hTERT mRNA and protein., Conclusion: Matrine can obviously inhibit cell cycle and down-regulate expression of hTERT. Inhibition of cell cycle is possible correlation with down-regulation expression of hTERT.

Purpose: The purpose of this study was to explore the diagnosis and operative methods for traumatic temporomandibular joint ankylosis., Methods: Fourteen patients (8 females, 6 males; aged from 7 to 23 years, median age 17.5 years) with traumatic TMJ ankylosis of 1 to 15 years' duration, with a maximal mouth opening(MMO) from 0 to 1.0cm(average:0.46cm) preoperatively were included in this study. They were further divided into two types(I and II) based on false articulation medial to the analysis as shown by coronal computed tomography (CT) coronoid scan. 7 TMJs were treated with medial arthroplasty(MA), in which the lateral fusional bone was removed and the medial false articulation was retained for type I, 6 TMJs were treated with natural positional arthroplasty(NPA) in which the bony fusion between the condyle and glenoid fossa was separated and shaved, the disc was retained and repositioned for type II. Follow-up was carried out and the surgical effect was assessed based on clinical and imaging findings. Student's t test was conducted for comparison with SPSS15.0 software package., Results: There was 9 TMJs from 7 patients for type I and 5 TMJs from 5 patients for type II as shown by CT. The mean postoperative MMO was 3.12cm, and the condylar shape was smooth on CT scan. There was significant difference between preoperative and postoperative MMO, P<0.01. There was no significant difference between MA and NPA, P>0.05., Conclusions: It is concluded that type I is more common in traumatic TMJ ankylosis than type II. The surgical effect of MA and NPA is good, with less injury, and is worthy of wide application.

Purpose: To investigate the effect and mechanism of matrine on adhesion and invasion of human adenoid cystic carcinoma ACC-M cells in vitro., Methods: MTT assay was used to examine the effect of matrine on proliferation of ACC-M cells treated after 1 day to 3 days. Byden chamber assay was performed to detect the effect of matrine on invasion capacity of the cells; Effect on adhesion potential of ACC-M cells was tested by cell matrigel adhesion assay. Mechanism of inhibitory adhesion and invasion was investigated by expression of E-cad protein. The results was analyzed by SPSS11.0 software package., Results: Matrine could inhibit the proliferation of ACC-M cell lines with apparent dose-dependent and time-dependent effect. Matrine significantly inhibited adhesion and invasion capacity of ACC-M cell lines in vitro with concentration increasing. The expression level of E-cad was significantly increased compared with the untreated group in ACC-M cell lines., Conclusions: Matrine can inhibit the adhesion and invasion capacity of ACC-M cell lines in vitro. Mechanism of the inhibition effect may be related to over expression of E-cad protein.

Objective: To study the molecular genetic etiology of a Chinese pedigree with basal cell nevus syndrome., Methods: The proband and his affected mother and a unaffected individual in the pedigree were chosen and peripheral blood was collected from them for DNA. Direct sequencing was performed to detect the mutations of PTCH gene. In order to further confirm the results of sequence analysis, all available family members were analyzed with genetic linkage analysis using 3 highly polymorphic microsatellite DNA markers in the region of 9q22.3-q31., Results: No mutations of PTCH gene was detected in the proband's mother, one synonymous mutation was detected in the proband. Linkage analysis showed that the Lod scores of the 3 markers were: D9S283, Z = -2.11 (theta = 0.00); D9S1690, Z = -2.95 (theta = 0.00); D9S1677, Z = -5.94 (theta = 0.00)., Conclusions: In this pedigree, mutation of PTCH gene is not related to the underlying pathogenesis of the syndrome.

Objective: To elucidate the molecular mechanism of X-linked adrenoleukodystrophy(ALD) in Chinese., Methods: Polymerase chain reaction in exon 1, exon 5 and their flanking sequences and direct DNA sequencing of ALD gene were performed in four patients, their mothers and twenty normal individuals as controls., Results: A splice mutation was identified in the interface of exon 5 and intron 5 (1875 G-->A). This splice mutation in 5' end of intron 5 might lead to abnormal splice in exon 5 and exon 6 and bring about unstable and abnormal ALD protein; the lignoceryl CoA ligase could not transport very long chain fatty acids (VLCFA) into peroxisome and could not function normally; consequently, defective beta-oxidation of VLCFA in peroxisome could result in an accumulation of VLCFAS in the central nervous system, adrenal gland and blood., Conclusion: The splice mutation in 5' end of intron 5 leading to abnormal splice in exon 5 and exon 6 appears to be one of the causes of X-linked recessive adrenoleukodystrophy.