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Based on the traditional black tea processing technology, the hot air withering process was used to replace the traditional indoor natural withering process on the basis of the traditional black tea processing technology. The effects of four hot air withering processes of 35 °, 45 °, 55 ° and 65 ° on the quality of summer and autumn black tea were studied and analyzed from three aspects of sensory quality, taste quality and aroma quality. The results show that compared with 25 ° traditional indoor natural withering black tea, the comprehensive sensory quality of black tea processed by 45 ° hot air withering process was better. The color of dry tea was dark brown and moist, the soup color was red and bright, the taste was mellow and refreshing, and the quality characteristics was sweet aroma. At the same time, the contents of tea polyphenols, gallic acid, EGCG, theaflavins, thearubigins and the ratio of the sum contents of theaflavins and thearubigins to the content of theabrownins (TFRB) significantly increased (P<0.05), while the content of soluble sugar decreased significantly (P<0.05). The types of aroma quality components increased, among which alcohols accounted for the highest proportion (70.47%), and the relative contents of alcohols, ketones and esters increased significantly (P<0.05), while pyrroles and aldehydes decreased significantly (P < 0.05). And the aroma components with flower and fruit aroma such as trans-3-hexenol, 2,3-dihydro-2,2,6-trimethylbenzaldehyde and α-ionone were detected. This shows that hot air withering with appropriate temperature is conducive to improving the quality of black tea, which can be applied to the production practice of improving the quality of black tea in summer and autumn. [ABSTRACT FROM AUTHOR]

With the aging of the population and the growing pressure of social competition, brain-related neurodegenerative diseases and mental disorders increasingly affect our quality of life. L-theanine is a unique non-protein amino acid in tea. Numerous studies have shown that L-theanine has significant effects on the prevention and treatment of brain diseases. In this paper, the absorption and metabolism of L-theanine in the body and the research results in the prevention and treatment of brain diseases were summarized, which would provide a reference for the application of L-theanine and development of functional products. [ABSTRACT FROM AUTHOR]

Objective: To diagnose a neonate presenting with multiple dysmorphic features, Cri-du-chat signs and hypoglycemia and to correlate the phenotype with the genotype., Methods: The patient was diagnosed with conventional cytogenetics and real-time fluorescence quantitative PCR (QF-PCR). The phenotype was then correlated with the genotype through a review of literature., Results: The neonate was diagnosed with a partial 13q trisomy (q12 → qter) and partial 5p monosomy (p15 →pter)., Conclusion: A rare diagnosis has been established with combined cytogenetic and molecular genetic techniques. QF-PCR has a broad application in genetic diagnosis.

Objective: To investigate the effects of genistein, a tyrosine protein kinase inhibitor, on the fenestrae, proliferation and nitric oxide (NO) synthesis of the liver sinusoidal endothelial cells from CCl(4)-induced hepatic fibrosis rats in vitro., Methods: By in situ collagenase perfusion and two-step percoll gradient centrifugation, SECs were isolated and cultured from normal and CCl(4)-treated Wistar rats. The fenestrae of SECs were observed by the scanning electron microscopy, and the SECs cell proliferation was determined by the MTT assay. The concentrations of NO in the cultured medium of SECs were detected indirectly by measurement of nitrates and nitrites (the stable products of NO) using the nitrate reduction method., Results: Scanning electron microscopic studies revealed that the number of fenestrae in SECs from all stage of hepatic fibrotic rats was decreased markedly as compared with the SECs from normal controls; however, no obvious changes in the fenestrae of SECs were observed after treated with genistein (100 mumol/L) for 24 hours. After treated with 100 mumol/L genistein for 24 hours, the cell proliferation rates of SECs from all stages of hepatic fibrosis were decreased significantly was compared with the control group (P<0.05). The synthesis of NO by SECs from all stages of hepatic fibrosis was markedly lower than those of normal controls. Treatment with 100 mumol/L genistein for 24 hours could increase the synthesis of NO by SECs from the early stage (stage I) of fibrosis; however, this effect of genistein was not observed in SECs from stage II or III of fibrosis at this concentration., Conclusions: The results suggest that genistein may play an important role in regulating the function of SECs.

Objective: To explore the expression of integrin alpha 6 in hepatic sinusoidal capillaration., Methods: The rat hepatic fibrosis model was established by injection of carbon tetrachloride subcutaneously. Then the expression of laminin and integrin alpha 6 subunit was observed by immunohistochemistry and dot immuno-blotting., Results: We observed sinusoidal capillaration formed by deposition of laminin along sinusoids in Disse interspace by immunohistochemistry staining. In normal rat the expression of integrin alpha 6 was restricted to portal vascular endothelial cells and bile duct epithelial cell membranes. No expression was observed in sinusoidal endothelial cell membranes. When capillaration integrin alpha 6 was detected in a continuous pattern along the sinusoids, the content of integrin alpha 6 was significantly higher in fibrotic liver tissues than in normal liver tissues as measured by dot immuno-blotting (P<0.05)., Conclusions: During fibrogenesis, laminin continuously accumulate in liver tissues and form basement membrane resulting in sinusoidal capillaration, and then induce the expression of integrin alpha 6 on SEC membranes.