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In order to clarify the main factors affecting the warmth retention of cold-proof textile and clothing products, the batting textile and clothing products were taken as example, with the help of YG(B) 606E temperature preservation instrument, the relationship between the characteristics of cold-proof clothing, washing and protective mode, parameter combination of washing and protective equipment and the relationship between detergent products and the warming property of cold-proof textile and garment products was systematically explored. The results show that in terms of product characteristics, the type and filling amount of flocculating materials were the main factors affecting the warmth of cold-proof textile and garment products (P < 0.01), while the coating materials (fabric, lining) had no significant effect (P > 0.05). In the aspect of daily washing and care process, the significant degree of influence was in the order of washing and care mode > washing and care equipment parameter combination > detergent products, and all of them were relatively significant (P < 0. 01), especially in the aspect of daily washing and care process of textile and clothing products with down material as the filling material, the trend was more obvious. The research results can provide reference for washing machine manufacturers to develop washing procedures suitable for batting textile and garment products, and also provide guidance for consumers to properly wash such products. [ABSTRACT FROM AUTHOR]

Objective: To screen the microRNAs involved in colon cancer proliferation and to investigate the expression and regulating function of target miRNA in colon cancer., Methods: Mitochondrial transcription factor A(TFAM), which was proved to be an oncogene to colon cancer in prior study, was used as target gene. The microRNAs involved in colon cancer proliferation were screened with miRWalk 2.0 software. The expression of screened miRNAs was examined in 30 samples of colon cancer tissue, para-cancer tissue, normal colon cell strain, and 3 colon cancer strains (SW480, HT-29, and HCT116) by real-time PCR. MiR-204 presenting lowest expression was selected to further study in SW480 cells. Dual luciferase reporter assays was performed to examine the association of TFAM with miR-204. Anti-miR-204 lentivirus and miR-240 lentivirus were used to down-regulate and up-regulate miR-204 expression respectively. Change of TFAM protein expression in SW480 cells was detected by Western blotting, and change of SW480 cells proliferation was detected by MTT and BrdU assay after lentivirus transfection., Results: After screening, the candidate miRNAs were miR-204, miR-211, miR-214, miR-381 and miR-590-3p. Expressions of miR-204, miR-211, miR-214 and miR-381 were lower, but miR-590-3p expression was higher, in colon cancer tissues than those in para-cancer tissues(all P<0.05). Meanwhile expressions of above 4 miRNAs(miR-204, miR-211, miR-214 and miR-381) were also lower, but miR-590-3p expression was higher as well, in SW480, HT-29 and HCT116 cells compared to normal colon cells(all P<0.05). Among above 4 miRNAs, miR-204 showed the lowest expression in both colon cancer tissues and cell lines. Expression of miR-204 was negatively correlated with TFAM expression in colon cancer tissues(P<0.05). Dual luciferase reporter assays revealed TFAM could be integrated with miR-204 directly, suggesting TFAM as the direct target of miR-204. After up-regulating miR-204 by lentivirus, expression of TFAM decreased and proliferation increased in SW480 cells(all P<0.05). After down-regulating miR-204 by lentivirus, expression of TFAM increased and proliferation decreased in SW480 cells(all P<0.05)., Conclusion: MiR-204 inhibits TFAM expression and up-regulates the proliferation of colon cancer cells SW480.