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Objective: To investigate the oxidation mechanism of walnut kernels. Methods: Walnut kernels were subjected to vacuum or non-vacuum packaging before accelerated oxidation at 60 ℃. Samples were taken periodically for ultrastructural and morphological observation by transmission electron microscopy (TEM) and scanning electron microscopy (SEM) and for the measurement of oxidation indicators such as peroxide value, carbonyl value, and cell membrane permeability. Changes in cellular ultrastructure during the oxidation process of walnut kernels were analyzed and compared under vacuum and non-vacuum packaging conditions. The oxidation process was assessed from a multi-dimensional perspective and the oxidation mechanism was explored as well. Results: Under non-vacuum conditions, walnut kernel oil began to become sticky on the 14th day of accelerated oxidation and then gradually became stickier. With oxidation time, intracellular protein bodies (PB) exhibited sequential changes including an increase in surface roughness, aggregation, movement toward the cell periphery, decomposition and reassembly. The oil body membrane (OBM) was ruptured and the intracellular reticulum was disrupted, thus causing oil leakage into the intercellular space and resulting in a reduction in the cell volume. Cellular structural changes were much smaller under vacuum conditions than under non-vacuum conditions, the oil started to become sticky on the 28th day, and the oxidation indicators were significantly lower than those under non-vacuum conditions (P < 0.01). The trend of changes in three oxidation indicators was consistent with that of cell ultrastructure. Conclusion: TEM and SEM combined with oxidation indicators can provide clearer insights into cellular ultrastructural changes in walnut kernels after oxidation. The oxidation mechanisms of vacuum and non-vacuum packaged walnut kernels are the same, but their oxidation degrees are different. Notably, vacuum packaging can significantly reduce the oxidation of walnut kernels.

Viral covert mortality disease (VCMD) in cultured shrimp is caused by covert mortality nodavirus (CMNV). The infected shrimp mainly die successively in the deep-water areas at the bottom of farming ponds, which is difficult to observe; as such, the term "covert mortality disease" was coined by local farmers. The disease is known as running mortality syndrome by Southeast Asian farmers because a small number of diseased shrimp die daily in the infected ponds, and more continue to die until harvest time. The infected shrimp mostly show symptoms such as carapace softening, hepatopancreas color fading, atrophy, empty stomach, slow growth, etc. In the acute infection stage, the infected shrimp show opaque abdominal muscles. The prevalence of VCMD has caused severe economic losses in the shrimp farming industry over the past decade. CMNV can infect many major cultured shrimp species, including Penaeus vannamei, P. japonicus, P. chinensis, Macrobrachium rosenbergii, and Exopalaemon carinicauda. Moreover, the virus infects the co-inhabiting organisms in shrimp ponds, which makes it difficult to remove from shrimp farming areas due to its wide host range among invertebrates and its transport by many vectors. To elucidate the prevalence of CMNV in the main cultured shrimp species in China in recent years, an epidemiological investigation of CMNV, including clinical sample collection from shrimp and other organisms inhabiting the shrimp ponds, was carried out in the main shrimp culture areas of China from 2021 to 2022, and the collected samples were systematically analyzed using molecular biology, histopathology, and transmission electron microscopy approaches. During this period, 1, 299 samples were collected from Tianjin, Shandong, Jiangsu, Hainan, Hubei, and Xinjiang and included P. vannamei, M. rosenbergii, P. japonicus, P. chinensis, Procambarus clarkia, and other organisms living in shrimp culture ponds. The collected samples were analyzed using CMNV TaqMan probe real-time quantitative reverse transcription PCR (TaqMan RT-qPCR). The results showed that CMNV could be detected in the main cultured shrimp species, including P. vannamei, M. rosenbergii, and P. japonicus. A percentage of the samples collected from Shandong, Jiangsu, Hainan, Xinjiang, Guangxi, and Tianjin were observed to be CMNV-positive in the TaqMan RT-qPCR assays. In addition to cultured shrimp, CMNV was detected in organisms living in the shrimp ponds, such as Artemia collected in 2021 and Perinereis aibuhitensis collected in 2022. The CMNV-positive rate of samples collected in 2021 was 10.04% (69/687), and that of samples collected in 2022 was 11.44% (70/612). Epidemiological investigation showed that CMNV infection in outdoor pond culture usually caused a certain degree of cumulative death, whereas CMNV infection in indoor culture mainly caused the softening and reduced growth of infected shrimp and did not cause a large number of infected shrimp to die. High CMNV loads were detected in samples of Artemia used as fresh bait for shrimp and in samples of P. aibuhitensis living in shrimp pond soils. Accordingly, a high CMNV infection rate was observed in cultured P. vannamei feeding on CMNV-positive Artemia and in that farmed in ponds containing CMNV-positive P. aibuhitensis. The results indicated that CMNV vectors such as Artemia and P. aibuhitensis may pose a high risk of CMNV transition, threatening the health of the shrimp in the culture system. Recent studies on the pathogenesis of CMNV infection indicated that the virus mainly infected epithelial tissues, nerve tissues, and connective tissues in the hepatopancreas, appendages, mid-gut, gills, eye stalks, and nerves of shrimp. Therefore, histopathological and in situ hybridization analyses were performed on samples identified as CMNV-positive using TaqMan RT-qPCR in this study. Eosinophilic virus inclusion bodies were observed in the hepatopancreas and foremidgut tissues, and pathological lesions were observed in the appendicular nerves of diseased Penaeus shrimp. Moreover, obvious purple hybridization signals from the CMNV RNA probe were seen in tissues with lesions, which further confirmed the positive detection results for CMNV in shrimp samples obtained using TaqMan RT-qPCR in this study. The results of this study showed that, compared with previous years, there was still a high CMNV-positive detection rate in many areas of crustacean culture in 2022, and the epidemic risk of this virus was still high. Multiple CMNV-positive results were obtained in the co-inhabiting and feed organisms in crustacean farming ponds. These results remind fishery authorities and stakeholders in the prawn breeding industry to further strengthen the monitoring and early detection of CMNV to prevent and control the spread of the virus in seedlings, reduce the risk of epidemic hazards, and promote the sustainable and healthy development of the crustacean aquaculture industry.

The tensile strength of clay is an important factor in determining the stability of soil slope. Due to the fragmentation of soil, the understanding of the tensile strength of clay is not clear. In this study, the uniaxial tensile test of remolded clay is carried out to analyze the influence of water content and tensile rate on the tensile strength of remolded clay based on the self-designed direct tensile test device. The test results show that: (1) The tensile strength of remolded clay increases first and then decreases with the increase of water content, as the other conditions (such as tensile rate) remain stable. The peak tensile strength occurs near the optimal water content. (2) The tensile strength of remolded clay increases with the increase of tensile rate, as other conditions such as water content remain unchanged. (3) In the water content range (18%–24%) of this study, the remolded clay undergoes plastic deformation under uniaxial tension, and shows softening failure law after reaching the peak tensile strength. (4) The discrete element simulation method is used to analyze and verify the results obtained from the above experiments. The results of this study can be used for the stability analysis of soil slope and the calculation and design of slope protection.

Objective: To determine the relationship between cartilage injury, bone marrow edema, and total bone mineral density in patients with knee osteoarthritis (KOA). Methods: Ninety patients with KOA admitted to the Sichuan Province Orthopedic Hospital between March 2023 and October 2023 were selected, all of whom underwent knee joint MRI and chest QCT examinations. The patients were divided into three groups according to the QTC results: normal bone density, reduced bone mass, and osteoporosis. Patient information, such as sex, age, body mass index (BMI), and course of illness, was also obtained. MRIs of the knee joints were scored separately according to the Recht grading and Whole-organ Magnetic Resonance Imaging Score (WORMS). The bone mineral density (BMD) of the patients was calculated and the BMD values were statistically analyzed using the MR score. Results: No statistically significant differences were observed in sex, BMI, or disease duration among the three groups; however, a statistically significant difference was observed in age. The Recht and WORMS scores among the three groups showed statistical significance between the normal bone density group and the reduced bone mass and osteoporosis groups; however, no statistical significance was observed between the reduced bone mass and osteoporosis groups. KOA cartilage injury was negatively correlated with bone density with a correlation coefficient of r=−0.412, while KOA bone marrow edema was negatively correlated with bone density with a correlation coefficient of r=−0.254. Conclusion: The total bone density in patients with KOA is related to knee joint cartilage injury and bone marrow edema. A decrease in total bone density may indicate the severity of knee cartilage injury or bone marrow edema.

Objective: This article aimed to analyze the computed tomography (CT) imaging manifestations and clinical significance of scimitar syndrome to improve our understanding of the disease. Method: A total of 28 patients diagnosed with scimitar syndrome were enrolled in our hospital from January 2019 to January 2024, and their clinical and imaging data were retrospectively analyzed. Results: Among the 28 cases, there were 10 males and 18 females, with 15 and 13 cases of the infantile and adult types, respectively. The average age of patients with the infant type were (6.78±3.50) months, with 10 cases having total anomalous right pulmonary venous drainage. Among them, three cases had stenosis and obstruction at the site of ectopic drainage and two cases had concomitant atrial septal defect and pulmonary arterial hypertension; these five cases underwent surgical treatment. Five cases of partial anomalous right pulmonary venous drainage to the inferior vena cava were found during imaging examination owing to the presence of other deformities and pulmonary infections. The average age of patients with the adult type was (5.53±2.72) years old. Seven cases had total anomalous right pulmonary venous drainage to the inferior vena cava, with one case undergoing surgical treatment. Six cases had ectopic drainage from the right lower pulmonary vein to the inferior vena cava: two underwent elective surgery owing to a clear diagnosis for many years, and two underwent interventional treatment for systemic arterial blood supply owing to extralobar sequestration. Conclusion: There were significant differences in clinical manifestation between patients with infantile and adult types of scimitar syndrome. Using multiple imaging modes of CT examination combined with powerful reconstruction post-processing functions, children with scimitar syndrome can be evaluated comprehensively, providing a basis for the selection of clinical treatment methods and surgical plans.

In recent years, ammonia (NH3) has received widespread attention as a fuel for internal combustion engines.Ammonia combustion produces a large amount of water, but incomplete combustion of ammonia may cause corrosion to engine piston components in medium to high temperature environments with water present.In this work, taking 4032 aluminum alloy piston material as the research object, the corrosion behavior in a 25%(mass fraction) ammonia solution was analyzed at a constant temperature of 200 ℃, and the corrosion weight loss, corrosion product morphology and corrosion electrochemical properties of aluminum alloys were explored emphatically at different time scales.Results showed that the corrosion rate of 4032 aluminum alloy in medium to high temperature ammonia environment exhibited a trend of first increasing and then decreasing with the prolongation of soaking time, and the corrosion products were mainly Al(OH)3.With the extension of soaking time, the corrosion potential Ecorr and polarization resistance Rp of 4032 aluminum alloy increased gradually, while the corrosion current density Jcorr decreased gradually, which further analyzed the corrosion mechanism.

"Objective To investigate the effect of visceral fat area (VFA) on carotid atherosclerotic plaque in patients with type 2 diabetes mellitus(T2DM). Methods From August 2018 to August 2023, 683 T2DM patients included in the database of the National Metabolic Management Center (MMC) were selected from the Department of Endocrinology, the Affiliated Geriatric Hospital of Nanjing Medical University. Medical history, physical examination, serum biochemical indicators, VFA and other data were collected from the study subjects. According to the presence or absence of carotid atherosclerotic plaque, all patients were divided into T2DM patients with plaque (n=342) and T2DM patients without plaque (n=341), and statistical analysis was performed. Results There were statistically significant differences in age, body mass index (BMI), waist circumference, diastolic blood pressure (DBP), total cholesterol (TC), low-density lipoprotein cholesterol (LDL-C), high-density lipoprotein cholesterol (HDL-C), abdominal subcutaneous fat area (SFA) and VFA between the two groups (PConclusion Age and VFA are independent influencing factors for atherosclerotic plaque formation in patients with T2DM，and as a new obesity assessment index, VFA can better predict and assess the risk of atherosclerotic plaque formation in T2DM patients."

High stress and multi faults lead to rising burst frequency in deep coal mine. Though backfilling mining serves as the most straightforward method for stress-decreasing and burst-preventing, it fails to eliminate coal burst completely. In order to decrease the influence of coal burst on safety mining of deep-buried seam with bursting liability, stress surge and drop mechanisms emerging in backfilling panel are studied with field measurement, theoretical analysis and laboratory test, and destressing effect of roof pre-blasting and large borehole drilling methods is analyzed. High stress, multi faults, width-changing pillar and mining disturbance leads to the emergence of stress concentration, stress surge and stress drop phenomenon in backfilling panel, but the concentration coefficient is smaller than 2.0. At the normal region, influence range reaches 30 m. It increases to 50 m when fault influence is considered and the value grows to 70 m if both fault and pillar influences are added to the panel. Fault activation and rock failure results in sudden release of strain energy, the mechanisms underlying the transition between strain energy and kinetic energy as well as dynamic stress wave are revealed. Note transition ratio reaches 17%. The superposition between dynamic stress wave and static stress field leads to sudden change in mining induced stress. Surrounding rock presents consolidation hardening behavior if it fails before load superposition, resulting in stress surge. Brittle caving behavior emerges if surrounding rock transits from intact into broken state, leading to stress drop. If surrounding rock still keeps intact after stress superposition, it presents elastic rebound behavior and experiences dynamic load. Large-scale rupture of hard roof is not observed due to supporting effect provided by backfilling materials and thus, microseismic energy associated with single event is smaller than 105 J. But microseicmic events presents asymmetrical distribution due to fault influences. They present high frequency and low energy mode at maingate side while low frequency and high energy mode is observed at tailgate side. As a result, burst monitoring pre-warning happens two times in the latter roadway and one time is observed in the former one. Hard roof pre-blasting and large borehole drilling methods are used to decrease mining-induced stress. Large-scale blasting fractures are formed in roof strata, which control stress concentration degree and stress increase speed effectively. Thus, coal burst danger is significantly decreased in deep mining.

In this study, the effects of Trichoderma erinaceum M6-5 volatile components on metabolites, respiration and energy metabolism of Alternaria alternata from postharvest Korla fragrant pear were investigated. Non-targeted metabolomics based on liquid chromatography-mass spectrometry (LC-MS) was used to determine the changes of metabolites in A. alternata after treatment with M6-5 volatile substances. At 0, 3, 5 and 7 days after fumigation with M6-5 volatile substances, the respiratory rate of A. alternata was measured, and the respiration inhibition rate were calculated. The levels of ATP, ADP, AMP and energy charge in the M6-5-treated and control groups as a function of culture time were determined by liquid chromatography (LC). The results showed that among the identified differential metabolites, 6-amyl-2h-pyrano-2-one (6pp), 4-ethylbenzaldehyde, 3,4-dimethylbenzoic acid, 4-phenylbutyric acid and 2-methoxyresorcinol were up-regulated, while 6-aminonicotinamide, 11-ketoacetyl cholesterol, 3-hydroxyconilone, jasmonic acid and benzoylsphenine were down-regulated by M6-5 volatile substances. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis showed that the significantly differential pathway was involved in the biosynthesis of secondary metabolites. At the end of the 7 day incubation period, the respiration inhibition rate of A. alternata by M6-5 volatile substances was (49.64 ± 2.04)%. Compared with the control group, treatment with M6-5 volatile substances significantly reduced the levels of ATP, ADP, AMP and energy charge, indicating that it can keep the energy charge at lower levels, thereby resulting in imbalanced energy metabolism and inhibited respiratory rate and affecting metabolite changes.

To study the surface morphology of Si3N4 ceramics using rotary ultrasonic grinding, changes in the surface morphology under different machining parameters were analyzed based on fractal theory. Orthogonal experiments were designed to compare and analyze the effects of various processing parameters on the fractal dimensions and multifractal spectra of the Si3N4 ceramic surface. Additionally, single-factor experiments were conducted to study the roughness, fractal dimensions, and multifractal spectra of the Si3N4 ceramic surface under different processing parameters. The results show that fractal dimensions can effectively characterize the defect state of the processed surface of Si3N4 ceramics during rotary ultrasonic grinding, while multifractal spectra can better represent the degree of fluctuation in surface defects.

To investigate the evolution of coal permeability during the process of gas pressure change, four different forms of permeability models were derived based on the correlation between mesoscale parameters of coal representative elementary volume (REV) and strain-related characteristics. The reliability of the models was verified using experimental data, and the variation law of strain caused by effective stress and adsorption strain was analyzed and compared with classical permeability models (P-M model, S-D model, Connell model). The sensitivity of model parameters was discussed, the relationship between REV mesoscale parameters and fracture porosity and permeability was clarified, and the influence mechanism of adsorption strain coefficient was analyzed. Furthermore, the extreme values of permeability of major coal mines in China were studied. Results show that for the four permeability models derived in this paper, the REV volume strain satisfies a linear relationship with its fracture volume strain, and the correlation coefficient is the initial fracture porosity. The bulk modulus and adsorption strain coefficient control the influence of effective stress and adsorption strain on permeability, respectively. Under three different experimental conditions of constant confining pressure, constant pore pressure, and constant differential pressure, the four permeability models have shown a better matching effect on the experimental data compared with the classical permeability models. However, compared with single-parameter fitting, the parameter error in the double-parameter fitting (bulk modulus and adsorption strain coefficient) is larger. From the sensitivity analysis of parameters, it is observed that when the parameters change within 10%, the predicted permeability deviates significantly from the true value. Therefore, for the double or multiple-parameter fitting of permeability models, the parameter fitting errors could lead to serious distortion of the predicted permeability results. Taking the experimental data of coal samples from the Qinshui Basin as an example, the mesoscopic scale parameters a0 and b0 of REV were calculated to range from 1.73 to 46.31 μm and from 0.06 to 0.49 μm, respectively. Under constant confining pressure and constant differential pressure conditions, the adsorption strain coefficient was related to differential pressure, gas types, and initial fracture porosity. Furthermore, the extreme values of CO2 gas and CH4 gas in major coal mines in China are within the ranges of 0.94−5.33 MPa and 1.06−6.94 MPa, respectively.

Objective To explore the current status of serum phosphorus level in patients on maintenance hemodialysis （MHD）, examine its relationship with clinical parameters and determine the influencing factors of serum phosphorus level. Methods For this multicenter cross-sectional study, 2667 patients on MHD at 27 domestic dialysis centers were recruited from 2019 to 2020. Demographic profiles, primary diseases and laboratory tests were recorded. According to serum phosphorus （P） level, they were assigned into three groups of low blood phosphorus （P

This is an article in the field of metallurgical engineering. Large production and storage capacity of phosphogypsum has become an important factor restricting the green and healthy development of phosphorus chemical industry. Removing impurity and whitening of phosphogypsum is a necessary way for its high consumption. Analysis results showed that there was still a small amount of black organic matter in the phosphogypsum after removing impurity and desilication by flotation, which affected its whiteness. In the paper, calcination and acid leaching were adopted to treat the phosphogysum. The whiteness of whitened phosphogysum significantly increased from 51.5% to 92.7% at the conditions of calcination temperature 600 ℃, calcination time 70 min, acid leaching time 2 h, sulfuric acid concentration 1.5 mol/L, acid leaching liquid to solid ratio 5∶1 and acid leaching temperature 90 ℃. The whitened phosphogypsum was mainly CaSO4, and contained a small amount of CaSO4·2H2O and CaSO4·0.5H2O. The particles of the whitened phosphogypsum were irregular in shape of 15～20 μm, and the surface was smooth. The study provided a guidance for the comprehensive utilization of phosphogypsum with high consumption.

Based on the geochemical parameters and analytical data, the heat conservation equation, mass balance law, Rayleigh fractionation model and other methods were used to quantify the in-situ yield and external flux of crust-derived helium, and the initial He concentration and thermal driving mechanism of mantle-derived helium, in the Ledong Diapir area, the Yinggehai Basin, in order to understand the genetic source, migration and accumulation mechanisms of helium under deep thermal fluid activities. The average content of mantle-derived He is only 0.001 4%, the 3He/4He value is (0.002–2.190)×10−6, and the R/Ra value ranges from 0.01 to 1.52, indicating the contribution of mantle-derived He is 0.09%–19.84%, while the proportion of crust-derived helium can reach over 80%. Quantitative analysis indicates that the crust-derived helium is dominated by external input, followed by in-situ production, in the Ledong diapir area. The crust- derived helium exhibits an in-situ 4He yield rate of (7.66– 7.95)×10−13 cm3/(a·g), an in-situ 4He yield of (4.10–4.25)× 10−4 cm3/g, and an external 4He influx of (5.84–9.06)×10−2 cm3/g. These results may be related to atmospheric recharge into formation fluid and deep rock-water interactions. The ratio of initial mole volume of 3He to enthalpy (W) is (0.004–0.018) ×10−11 cm3/J, and the heat contribution from the deep mantle (XM) accounts for 7.63%–36.18%, indicating that deep hot fluid activities drive the migration of mantle-derived 3He. The primary helium migration depends on advection, while the secondary migration is controlled by hydrothermal degassing and gas-liquid separation. From deep to shallow layers, the CO2/3He value rises from 1.34×109 to 486×109, indicating large amount of CO2 has escaped. Under the influence of deep thermal fluid, helium migration and accumulation mechanisms include: deep heat driven diffusion, advection release, vertical hydrothermal degassing, shallow lateral migration, accumulation in traps far from faults, partial pressure balance and sealing capability.

Fine terrain classification is one of the main applications of Synthetic Aperture Radar (SAR). In the multiband fully polarized SAR operating mode, obtaining information on different frequency bands of the target and polarization response characteristics of a target is possible, which can improve target classification accuracy. However, the existing datasets at home and abroad only have low-resolution fully polarized classification data for individual bands, limited regions, and small samples. Thus, a multidimensional SAR dataset from Hainan is used to construct a multiband fully polarized fine classification dataset with ample sample size, diverse land cover categories, and high classification reliability. This dataset will promote the development of multiband fully polarized SAR classification applications, supported by the high-resolution aerial observation system application calibration and verification project. This paper provides an overview of the composition of the dataset, and describes the information and dataset production methods for the first batch of published data (MPOLSAR-1.0). Furthermore, this study presents the preliminary classification experimental results based on the polarization feature classification and classical machine learning classification methods, providing support for the sharing and application of the dataset.

The pH and firmness index (FI) of red globe grapes determine the taste and post-harvest quality of the fruit. In this paper, a method for the detection of pH and firmness of red globe grapes based on transmission spectroscopy technology was proposed. Spectral datas were first collected from 360 full-growth-cycle red globe grape samples, which were pre-processed and modeled by different spectral pre-processing methods as a way to determine the best spectral pre-processing method. Then competitive adaptive reweighted sampling (CARS), successive projection algorithm (SPA), uniformative variable elimination (UVE) and CARS-SPA, UVE-SPA composite data dimensionality reduction methods were used respectively for extracting feature variables from spectra. Finally, partial least squares regression (PLSR) detection models for pH and firmness of red globe grapes were established, respectively. The optimal prediction models for pH and firmness of red globe grape samples were moving-average method (MA)-CARS-SPA-PLSR and MA-UVE-SPA-PLSR. The correlation coefficient of prediction (RP) of the prediction sets of the two models were 0.9882 and 0.9588, and the residual predictive deviation (RPD) were 6.5857 and 3.5167, respectively. The results showed that transmission spectroscopy could be applied to the detection of pH and firmness of red globe grapes, which provided a new idea and a new method for the detection of pH and firmness of red globe grapes in the whole growth cycle.

Zein is a primary by-product of corn starch processing, which is inexpensive and easily obtainable. Based on its unique self-assembly properties, zein could be constructed into nanoparticles through various methods, enabling the loading and delivery of bioactive substances. The preparation methods of nanoparticles include anti-solvent precipitation, pH-driven, electrospray, and microfluidics. The preparation methods can produce zein-based nanoparticles that respond to various environmental stimuli and exhibit stable properties. This article reviews recent advances in the preparation methods, response types, and application progress of stimulus-responsive zein-based nanoparticles. The nanoparticles respond to endogenous (pH, enzymes, and redox) and exogenous environmental stimuli (magnetic and light), which have been used for encapsulated delivery of bioactive substances in smart packaging of food, nanopesticides, and nanomedicine. However, maximizing the effect of active substances is difficult to achieve by relying only on a single stimulus-response. Therefore, nanoparticles responding to multiple stimuli (double stimulation, triple stimulation, etc.) have become a new development trend in the delivery field. In the future, more research should be invested in developing multi-stimulus responsive nanoparticles, and how to combine the multiple response properties of nanoparticles with the targeted delivery of nanoparticles remains to be further explored.

This article reports a case of acute myelomonocytic leukemia (AML-M4, CBFB gene positive) with lower limb microthrombus formation after haploidentical hematopoietic stem cell transplantation, and summarizes the experience of diagnosis and treatment. The patient received achieved complete remission (CR) after routine chemotherapy, but relapsed 2 years later and underwent a haploidentical hematopoietic stem cell transplantation. During routine treatment after transplantation, the patient’s coagulation related indicators, including D-dimer (D-D), antithrombin Ⅲ (AT-Ⅲ), von Willebrand factor (vWF), fibrinogen degradation products (FDP) and fibrin monomer (FM), were dynamically observed. The patient experienced jaundice and elevated transaminases around 100 days after transplantation, and underwent plasma exchange treatment three times through the right lower limb femoral vein catheterization. After removing the venous catheter for one week, there was left ankle concavea pitting edema of the left ankle. Vascular ultrasound examination indicated the formation of microthrombus in the superficial venous plexus of the gastrocnemius muscle in the left lower limb. D-D, vWF, FDP, FM and other indicators were increased, while AT-Ⅲ levels was decreased. After 2 weeks of oral administration of 5 mg/d berritol for antithrombotic treatment, the above coagulation indicators gradually returned to normal, and the left lower limb intermuscular venous plexus thrombosis and limb swelling disappeared. Therefore, dynamic monitoring of coagulation indicators, such as D-D, AT-Ⅲ, vWF, FDP, and FM may serve as diagnostic indicators for predict transplant related microthrombus and hypercoagulable status, providing an experimental basis for effective anticoagulant treatment in clinical practice.

To study the damage and failure mechanism of rocks under the coupling effect of high ground stress static load and cyclic impact disturbance generated by mining and excavation, the multi-strain rate dynamic static superposition rock mechanics test system was used to carry out the experiments with different pre-imposed static loads (0.45/0.65/0.85σc) superimposed cyclic impact and the same pre-imposed static load superimposed with cyclic impact loads of different frequencies (0.5/1.0/2.0 Hz). The experimental results indicate that the peak strength of rocks in the dynamic static superposition test is smaller than that in the static load test, and the maximum deformation is greater than that in the static load test, indicating that the dynamic static superposition load has a significant promoting effect on rock damage. The evolution of strength, deformation, and failure under dynamic and static superimposed loads are consistent, also, peak strength, fracture duration are linearly negatively correlated with pre-loading static, and logarithmically positively correlated with cyclic impact frequency. The maximum strain, fracture fractal dimension, and fragment fractal dimension are linearly positively correlated with pre-loading static, and logarithmically negatively correlated with cyclic impact frequency. Under different dynamic and static superpositions, the evolution trend of the fractal dimension of rock surface cracks and fragment sizes are basically consistent, and the former is larger than the latter, that shows the synchronicity of the development of rock surface and internal cracks, and rock surface cracks are more prone to generation and expansion. As the pre-loading static increases or the impact frequency decreases, the rock failure gradually intensifies, and the failure mode undergoes a transition from “inclined shear failure to vertical tensile failure to overall burst failure”. The burst failure position extends from bottom to overall. To quantify the damage mechanism of pre-loading static and cyclic impact, a dynamic static superimposed damage factor evolution model was established, which comprehensively considers static load damage, different peak, frequency, and number of cyclic impact damage, and strain rate strengthening effects. Further dynamic and static superposition experiments were conducted, and the error rates of rock peak strength obtained from theoretical calculations and experimental results were 0.5%, 1.8%, 0.6%, and 1.7%, respectively, the errors were relatively small. The theoretical calculation strength based on the superposition of dynamic and static damage factors is lower than the experimental strength. Preliminary analysis shows that this is due to the microscopic hysteresis of damage development under high-frequency cyclic impact. The actual cumulative damage generated by cyclic impact is less than single impact damage multiplied by cycle number. In the later stage, the microscopic testing can be carried out to explore the evolution law of rock microscopic damage under cyclic impact and further improve the theoretical model.

High stress is the main reason for high frequency of rock burst in deep coal mines. Backfilling mining serves as an effective method to control roof strata movement, alleviate the degree of stress concentration, and reduce the failure of surrounding rock and the risk of rock burst. To evaluate the controlling ability of the backfilling on mining stress in the gob area, the 1123 longwall panel of the Gucheng mine is taken as the project background. Theoretical analysis, laboratory test and field measurement are used to study the mining-induced stress distribution in the backfilling longwall face, and to reveal the feedback mechanism between the front abutment stress and bearing capacity of the backfilling body, helping to determine the backfilling rate of coal burst mine. The results show that the backfilling ratio in the early stage is lower than 80%, the subsidence of hard roof is serious, the influence area of front abutment stress is more than 30 m, and the degree of stress concentration reaches 1.5. The influence area and concentration degree of mining-induced stress in the fault affected area increase to 60 m and 1.65, respectively. Surrounding rock instability phenomenon, such as rib spalling and roof fall, happens frequently. The whole dynamic evolution characteristics of bearing stress in the backfilling body is measured. The stress distribution curve is divided into five stages, namely rapid decrease, transient stabilization, rapid increase, slow decrease, and secondary stabilization. The stabilization values of the bearing stress in the upper, middle, and lower regions of the gob area under the condition of low backfilling ratio are 1.5, 5.2 and 2.5 MPa, respectively. The backfilling body in the gob area is divided into non-sufficiently compacted zones and sufficiently compacted zones, and the continuous subsidence model of the hard roof under the support of the backfilling body is constructed, and the “ʅ” type subsidence curve of the hard roof is obtained, and the range of the insufficiently compacted area decreases approximately linearly with the backfilling ratio. The test obtains the evolution curves of elastic modulus and uniaxial compressive strength of the back-filling body with time. By combining with the roof subsidence curve and face advance speed, the distribution curve of mining-induced stress in the whole area of the longwall face is obtained. Negative exponential relationship is established for the backfilling rate and the afront abutment stress, which reveals the reverse feedback mechanism between them. Quantitative assessment on the load reduction and burst prevention effect of backfilling mining is realized. The trinity enhancement measures for improving the backfilling ratio of the gob area are proposed, which raises the backfilling ratio of the 1123 working face to more than 90%. The bearing capacity of the backfilling body is strengthened. The trinity measures reduce the separation space under the hard roof to 50 mm. The stress concentration coefficient decreases to 1.3. Correspondingly, the controlling effect of the surrounding rock of the longwall panel is significantly improved.

Localization and mapping of the underground ramps is one of the key technologies for achieving unmanned driving of underground mining trucks. The underground ramp is a typical unstructured environmental feature, and the road has a certain inclination angle. However, the traditional SLAM algorithm cannot obtain accurate odometer information, which makes the accuracy of positioning and mapping difficult to meet the driving requirements of unmanned mine trucks. To overcome above problems, this paper proposes a positioning and mapping method suitable for underground ramp environments in mines by studying the laser SLAM (Simultaneous Localization And Mapping) algorithm LeGO-LOAM. Firstly, in terms of the characteristics of smooth cement walls and sparse features on both sides of the underground ramp, a manual landmark-based auxiliary enhancement positioning method is designed, effectively increase the number of point cloud features, to optimize the pose estimation results and avoid the phenomenon of mapping drift. Then, in the feature preprocessing stage, a highly efficient algorithm for extracting ground points based on the fusion of laser point cloud height difference and slope information is proposed, by improving the selection strategy of ground points, which can effectively be identified for sloped potholes, to solve the problem of large inclination angle and error in underground ramps. Next, a slope point cloud surface voxel clustering algorithm based on the CVC (Curved-Voxel Clustering) clustering algorithm is designed, using the curvature voxels and hash-based data structures to segmentation of point clouds, which significantly improve the robustness of point cloud clustering in sparse and noisy environments underground. Finally, the Scan-To-Map is used for point cloud registration, while balancing the performance and speed of point cloud alignment. The on-site experiment in an underground ramp of Shandong, Sinosteel Group proved that the proposed method has improved the accuracy by 13.15% and reduced the Z-axis error by 22.3% compared with the original algorithm. The quality of the map has been significantly improved, which can effectively solve the problem of mapping and positioning in underground autonomous driving.

With the advancement of wireless sensing technology, research on Wi-Fi-based identity recognition has garnered significant attention in fields such as human-computer interaction and home security.While identity recognition based on Wi-Fi signals has achieved initial success, it is currently primarily suitable for scenarios involving individual user behavior.Identity recognition for multiple users in concurrent behavior scenarios still faces a series of challenges, including issues related to mutual interference between users and poor model robustness.Therefore, a Wiblack system for recognizing multiple user identities in a concurrent distribution behavior scenario was proposed.The core idea was to train a multi-branch deep neural network (Wiblack-Net) to extract unique features for each individual user.Firstly, the common features among multiple users were extracted using the backbone network.Then, a binary classifier was assigned to each user to determine the presence of the target user within a given group, thereby achieving identity recognition for multiple users based on concurrent behavior.In addition, experiments comparing Wiblack with several independent binary classification models and a single multiclassification model were conducted to analyze operational efficiency.System performance experimental results demonstrate that when simultaneously identifying the identities of three users, Wibalck achieves an average accuracy of 92.97%, an average precision of 93.71%, an average recall of 93.24%, and an average F1 score of 92.43%.

Visual fatigue is an eye discomfort caused by a variety of factors and affects a wide range of the population. Studies have shown that reasonable dietary supplements can effectively improve eye discomfort caused by visual fatigue and even reduce the occurrence of visual fatigue. At present, the mechanisms of action of carotenoids represented by lutein in relieving visual fatigue has not been studied deeply, and few reviews have been conducted on their application in health foods for relieving visual fatigue. Therefore, this article describes the pathways of digestion, absorption, and metabolism of carotenoids in the human body, and reviews recent progress in carotenoids for relieving visual fatigue. Also, this article provides a statistical analysis of the application of carotenoid-rich ingredients in health foods for relieving visual fatigue in China, with a view to providing a reference and inspiration for researchers to develop health foods for relieving visual fatigue.

Mining key nodes in complex networks has been a hotly debated topic as it played an important role in solving real-world problems.However, the existing key node mining algorithms focused on finding key nodes from a global perspective.This approach became problematic for large-scale social networks due to the unacceptable storage and computing resource overhead and the inability to utilize known query node information.A key node mining algorithm based on multiple query nodes was proposed to address the issue of key suspect mining.In this method, the known suspects were treated as query nodes, and the local topology was extracted.By calculating the critical degree of non-query nodes in the local topology, nodes with higher critical degrees were selected for recommendation.Aiming to overcome the high computational complexity of key node mining and the difficulty of effectively utilizing known query node information in existing methods, a two-stage key node mining algorithm based on multi-query was proposed to integrate the local topology information and the global node aggregation feature information of multiple query nodes.It reduced the calculation range from global to local and quantified the criticality of related nodes.Specifically, the local topology of multiple query nodes was obtained using the random walk algorithm with restart strategy.An unsupervised graph neural network model was constructed based on the graphsage model to obtain the embedding vector of nodes.The model combined the unique characteristics of nodes with the aggregation characteristics of neighbors to generate the embedding vector, providing input for similarity calculations in the algorithm framework.Finally, the criticality of nodes in the local topology was measured based on their similarity to the features of the query nodes.Experimental results demonstrated that the proposed algorithm outperformed traditional key node mining algorithms in terms of time efficiency and result effectiveness.

In recent years, there has been widespread attention on hypergraphs as a research hotspot in network science.The unique structure of hypergraphs, which differs from traditional graphs, is characterized by hyperedges that can connect multiple nodes simultaneously, resulting in more complex and higher-order relationships.Effectively identifying important nodes and hyperedges in such network structures poses a key challenge.Eigenvector centrality, a common metric, has limitations in its application due to its locality when dealing with hub nodes with extremely high degree values in the network.To address this issue, the hypergraphs were transformed into their corresponding line graphs, and non-backtracking matrix centrality was employed as a method to measure the importance of hyperedges.This approach demonstrated better uniformity and differentiation in assessing the importance of hyperedges.Furthermore, the application of both eigenvector centrality and non-backtracking matrix centrality in assessing the importance of nodes in hypergraphs was explored.Comparative analysis revealed that non-backtracking matrix centrality effectively distinguished the importance of nodes.This research encompassed theoretical analysis, model construction, and empirical studies on real-world data.To validate the proposed method and conclusion, six real-world hypergraphs were selected as experimental subjects.The application of these methods to these hypergraphs confirmed the effectiveness of non-backtracking matrix centrality in identifying important nodes and hyperedges.The findings of this research offer a fresh perspective and approach for identifying key elements in hypergraphs, holding significant theoretical and practical implications for understanding and analyzing complex network systems.

This is an article in the field of metallurgical engineering. A copper cobalt ore Congo is an oxide ore with Cu 3.43% and Co 0.42% respectively. In this paper, the five stage cycle leaching process of leaching solution was used for the copper and cobalt leaching. The optimum conditions were as follows: the amount of sulfuric acid was 7.4% of the ore mass, the amount of sodium sulfite was 1.68 times of the theoretical amount, the grinding particle size of -74 μm accounts for 75%, leaching temperature was 45 ℃, the single-stage leaching time was 4 hours, and the liquid-solid volume mass ratio was between 2/1 and 3/1, the results showed that the leaching rate of copper and cobalt were 96.85% and 95.67% respectively. According to this leaching process, the amount of sulfuric acid was reduced by 6 kg/t compared with the primary leaching, and the total solution amount in the leaching process was reduced by about 1/4, which made the amount of subsequent cobalt precipitation and copper extraction solution was reduced.

Aiming at the comprehensive application of marine relay equipment, combined with the maximum communication distance and target threat distribution and other conditions, this paper proposes an programming model to solve the optimal deployment of communication relay platform array. This model is based on the principle of the shortest distance between the route starting point and the array postion point of the relay platform. It models the array deployment problem of the relay platform under the constraints of communication distance and target threat, and uses the convex optimization method to obtain the analytical form of the optimal solution. The optimal solution has strong interpretability, and the solution process avoids using traditional iterative or heuristic methods, which has the characteristics of small calculation. The example analysis shows that the optimal solution of the array deployment can be obtained quickly for the different position distribution of the mission platform and the threat target.

Objective To investigate the correlation between interleukin(IL) -1 family, classical inflammatory markers and pulmonary function in patients with stable chronic obstructive pulmonary disease(COPD). Methods Forty-five patients with stable COPD who visited the outpatient clinic or ward of the Department of Geriatrics,Beijing Jishuitan Hospital,Capital Medical University,from July 2021 to December 2022 were selected for a prospective cross-sectional survey to collect gender,age,smoking history and smoking index,underlying disease information,and serum IL-1 family(IL-1α,IL-1β,IL-18),IL-6,IL-8,TNF-α detection and pulmonary function tests. Results Multivariate regression analysis found that IL-18 was independently correlated with forced expiratory volume in the first second(FEV1) predicted value(FEV1%),IL-18 and age were independently associated with the single-breath diffusing lung capacity of carbon monoxide(DLCO),IL-18 was independently related to the improvement rate of FEV1. Conclusion IL-18 level in stable COPD was significantly correlated with pulmonary ventilation function,pulmonary diffusion capacity and FEV1 improvement rate,which suggesting that IL-18 may play an important role in the development of the disease at this stage and can be used as the good monitoring indicators for the evaluation of stable COPD.

Bare footprint recognition technology is a branch of image recognition technology, which plays an important role in criminal investigation, medical treatment and security fields, and is expected to become a new means of personal identification. However, this technology has not yet formed a relatively unified framework, nor has it established a standardized procedure. In order to provide guidance for future researchers, it is necessary to standardize the recognition process of different bare footprint images and summarize the relevant research of bare footprint recognition technology. Firstly, the background and significance of bare footprint recognition research are expounded. Then, the development history of this technology is reviewed, and the bare footprint images are divided into four categories according to different acquisition methods: ink stamped bare footprint images, plantar scanning images, footprint images acquired by optical footprint acquisition equipment and foot pressure images acquired by footprint pressure acquisition system. It is pointed out that the latter two images are the hot spots of bare footprint recognition research at present. Then, the research status of bare footprint recognition technology is analyzed from three aspects: dataset, image preprocessing and recognition methods. Among them, the recognition methods are divided into traditional methods and deep learning-based methods, and the latter is further divided into network structure innovation methods and loss function optimization methods. The evaluation indices of identification methods are given, and various methods are compared from many aspects. Finally, the problems faced by this technology are pointed out, and its future development direction is prospected.

Objective To observe the effects of astragaloside （Ast）-incubated human adipose derived stem cell （hADSC） on diabetic kidney disease （DM） after caudal vein transplantation. examine the repair status of diabetic kidney disease （DKD） on renal injury in rats and elucidate its possible mechanism. Methods A total of 21 Sprague-Dawley rats were randomized into four groups of normal （n = 5）, model （n = 6）, hADSC （n = 5） and Ast-hADSC （n = 5）. DKD model was established in the last 3 groups. Both hADSC and Ast-hADSC groups were injected 6 times with hADSC or Ast-hADSC suspension through tail vein once biweekly. The model group received the same volume of normal saline. Renal function, blood lipid, urinary microalbumin/urinary creatinine and urinary protein quantification were detected. Renal pathology was observed after hematoxylin-eosin and Masson stains and renal colonization of hADSC by confocal laser. Immunohistochemistry and immunoblot were utilized for observing the voltage-dependent anion channel （VDAC） and microtubule-associated protein-light-chain-3. LC3Ⅱ/LC3Ⅰ, autophagy substrate P62 protein, phosphatase and tensin homology induced putative kinase 1 （Pink1） and E3 ubiquitin ligase （Parkin） protein expression. Mitochondria and autophagosomes were observed by transmission electron microscopy. Results （1） As compared with model group, total serum cholesterol [（2.65 ± 0.04） mmol/L, （2.30 ± 0.29） mmol/L vs （3.31 ± 0.91） mmol/L], ratio of urinary microalbumin/urinary creatinine [（58.91 ± 11.86） mg, （44.00 ± 23.82） mg vs （101.03 ± 46.88） mg] and 24 h urinary protein quantity [（321.18 ± 88.71） mg, （136.35 ± 66.01） mg vs （408.67 ± 113.95） mg] （P＜0.05） declined in hADSC and Ast-hADSC groups. Body weight gained [（240.20 ± 26.43） g, （274.00±17.89） g vs （193.60 ± 18.57） g] （P＜0.05）. The effects of body weight and 24 h urinary protein were more significant in Ast-hADSC group than those in hADSC group （P＜0.05）. （2） As compared with model group, no significant differences existed in triglyceride, low density lipoprotein, urea nitrogen or high density lipoprotein in hADSC and Ast-hADSC groups （P＞0.05）. （3） Both hADSC and Ast-hADSC groups had lesser renal injury while renal index and glomerular cross-sectional area spiked （P＜0.05）; Improvement was more significant in Ast-hADSC group. （4） Under laser confocal microscope, minimal red fluorescence was observed in hADSC and Ast-hADSC groups. （5） Immunohistochemistry and immunoblotting revealed that protein expressions of LC3, LC3Ⅱ/LC3Ⅰ, Pink1 and Parkin spiked in hADSC and Ast-hADSC groups （P＜0.05） while protein expression of P62 declined （P＜0.05）. The effect of Ast-hADSCs group was more significant. （6） In model group, mitochondria were swollen, deformed and fragmented and the number of autophagosomes was fewer. Both hADSC and Ast-hADSC groups showed more autophagosomes and fewer swollen deformed mitochondria and mitochondrial fragments. The effect of Ast-hADSC group was more significant. Conclusions hADSC may restore mitochondrial autophagy in kidney tissue of DKD rats and reduce kidney injury. The effect is more significant after Ast incubation.

BackgroundPolycyclic aromatic hydrocarbons (PAHs), one of the main components of fine particulate matter (PM2.5), have a certain impact on ambient air quality, and long-term exposure to PAHs may pose potential health risks to human beings. ObjectiveTo identify the distribution characteristics and sources of PAHs in atmospheric PM2.5 in a district of Taizhou City from 2019 to 2021, and to evaluate the health risks of PAHs to the population in the area through the inhalation pathway. MethodsFrom 2019 to 2021, air PM2.5 sampling was carried out at a state-controlled surveillance point in a district of Taizhou City for 7 consecutive days on the 10th-16th of each month, the sampling time was 24 h·d−1, and the sampling flow rate was 100 L·min−1. PM2.5 mass concentration was calculated by gravimetric method. A total of 16 PAHs were determined by ultrasonic extraction-liquid chromatography. Kruskal-Wallis H test was used to compare the distribution charac teristics of PAHs concentrations by years and seasons, characteristic ratio and principal component analysis (PCA) was used to analyze their sources, and a lifetime carcinogenic risk (ILCR) model was used to assess the health risk of PAHs. ResultsFrom 2019 to 2021, the annual average concentrations [M (P25, P75)] of ∑PAHs in atmospheric PM2.5 in the selected district of Taizhou City were 6.52 (2.46, 10.59), 8.52 (4.56, 12.29), and 3.72 (1.51, 7.11) ng·m−3, respectively, and the annual benzo[a]pyrene (BaP) excess rates (national limit: 1 ng·m−3) were 27.38% (23/84), 47.62% (40/84), and 19.04% (16/84), respectively, both presenting 2020> 2019 > 2021 (P

To achieve full-band observation of solar radio emissions, the sun-planetary interstellar monitoring chain subsystem of the Phase II of Chinese Meridian Project will construct four sets of solar radio spectrometers covering the decimeter, meter, decameter, and centimeter wavebands, providing reliable data support for solar physics and space weather research and operations. The system configuration and main technical parameters of the full-band radio spectrometer are introduced, and the data products and calibration process described.

Nutrients in human milk are influenced by maternal diet, but it is still clear which type(s) of nutrients can be attributed to dietary changes. Most studies do not directly assess maternal diet or quantify the relationship between maternal diet and human milk composition. In this study, we retrieved and analyzed 24 observational studies, 5 crossover experimental studies and 11 parallel controlled experimental studies concerning ‘human milk/breast milk’, ‘nutrient’, ‘macronutrient’, ‘micronutrient’, ‘maternal nutrition’ and ‘maternal diet’ from the literature published before May 2022 in the PubMed and the Web of Science databases. It was found that in the diet of lactating mothers, fatty acids, especially polyunsaturated and trans fatty acids, and certain micronutrients, including fat-soluble vitamins (vitamins A and D), water-soluble vitamins (vitamin C) and trace minerals (iron, zinc and selenium), were closely related to their contents in human milk.

In order to study the characteristics and quality changes of hot air drying (HAD) of prune, fresh prunes were used as raw material to investigate the quality changes under five different blanching conditions, and to investigate the changes of colour and aroma of prune during the hot-air drying process with different temperatures and air speeds. The results showed that the blanching temperature of 90 ℃ and the blanching time of 90 s could maintain better VC and anthocyanin contents (1.13, 12.42 mg/100 g) and inhibit peroxidase (POD) activity and polyphenol oxidase (PPO) activity (0.99 ∆OD470/min·mg, 0.57 ∆OD420/min·mg). The effect of drying temperature on time was more significant than that of air speed on time (P

To address the problems of low retrieval efficiency of searchable encryption scheme under traditional public key cryptosystem, high storage overhead of keyword index, and index does not support dynamic update, based on the good space and time efficiency of counting Bloom filter to determine whether an element belongs to a certain set, the feature into searchable encryption was applied to establish keyword index, thus, the keyword set was mapped into a binary vector, thereby reducing the keyword storage cost.Simultaneously, the feature of counting Bloom filter can be deleted was used to realize the dynamic update of keyword index.In addition, to prevent adversaries from inferring keyword plaintext information from binary vectors through statistical analysis, the binary vector was blinded by permutation, and then the permutation was encrypted by attribute-based encryption for secret sharing.After legitimate users generated keyword traps correctly, the service provider helped users complete keyword matching queries based on the user’s keyword trapdoor.Finally, the security and efficiency of the scheme were proved through security analysis and simulation experiments.

To reveal the evolution law of the failure depth of the floor under Group A coal seam with regional grouting in Huainan Mining Area, based on the geology and grouting process data of 12123 working face in Pan’er Coal Mine, the mining response under two working conditions of no-grouting and grouting was simulated and analyzed. The failure depth of the floor under different grouting radius was compared. According to the microseismic (MS) monitoring in the mining process, the cluster analysis of its concentration range was carried out by SPSS software. The floor failure from MS monitoring in the adjacent 1612A working face with similar geological conditions and without grouting was discussed. The research showed that, the failure depth of the floor under the grouting condition was significantly less than that without grouting, which preliminarily verified the grouting effect to maintain floor stability. The actual grouting radius in the floor area was 25-30 m. The difference of failure types before and after actual grouting was revealed, and the grouting effect on floor failure was verified again.

Upper Ordovician Wufeng to Lower Silurian Longmaxi formations in Nanchuan region, as an important shale gas productive layer, is characterized by large shale thickness, deep burial depth, and complex in-situ stress with rapid direction changes. Therefore, the study of in-situ stress field is of significance for effective deve-lopment of shale gas in the study area. In order to clarify the characteristics and distribution of the in-situ stress field, the SHELLS finite element stress field modeling method, with faults, topography, heat flow, petrophysical parameters and boundary conditions as constraints, was used in the study of the stress field in the Wufeng-Longmaxi formations in the Nanchuan region.The results indicate that the maximum compressive horizontal principal stress is in thrust regime and that there are four principal stress directions and regions in general: NW-SE, NE-SW, near EW and near SN. The strain rate is in thrust regime, with three regions of low strain rate (magnitude ≤ -18), medium strain rate (magnitude between -18 and -17.6) and high strain rate (magnitude ≥ -17.6) and their corresponding NE-SW, NW-SE, SN and EW spreading directions. The fault slip rate regime is in thrust, the fault slip rates range from 0 to 0.001 2 mm/a. The modeled results of the maximum compressive horizontal principal stress, the strain rate and fault slip rate were compared with the measured data respectively, including the maximum compressive horizontal principal stress directions measured in drilled wells, the regime and magnitude of strain rate in Guizhou and Chongqing, and the properties of regional faults. The modeled results showed high agreement with the measured data, indicating the accuracy of the predicted results. Finally, based on fracture openness and fracture development revealed by modeled results, favorable fractured reservoir development zones for shale gas was evaluated, and class Ⅰ and Ⅱ zones for further exploration and development were predicted.

The Dongsheng structural belt in Nanchuan area, southeast Chongqing, is located in the transitional zone of the southeastern margin of Sichuan Basin. It has experienced multi-period tectonic reformation and has typical geological characteristics of complex structure, complex preservation conditions and complex in-situ stress field, which leads to a significant difference in single well production. In order to increase the single well production of normal pressure shale gas wells in Dongsheng complex structural belt and realize profitable development, through target processing of seismic data, analysis of structural characteristics, study of accumulation regularity and analysis of production dynamics, as well as the strengthening of basic research and exploration and development practice, four key technical countermeasures for realizing profitable development of normal pressure shale gas in Dongsheng complex structural belt are defined: (1) improve the accuracy of shallow velocity model and structure interpretation; (2) optimize well pattern deployment, increase well-controlled reserves; (3) integrate geology and engineering, and coordinate drilling, orientation and steering to improve the drilling rate of sweet spots; (4) establish a zonal differentiated fracturing scheme to improve fracture complexity. These four countermea-sures provide technical support for the profitable development of normal pressure shale gas in complex structural zones, and provide reference for the exploration and development of other similar areas along the basin margin.

ObjectiveTo evaluate the effect of COVID-19 in patients with membranous nephropathy (MN). MethodsFrom December 15, 2022 to January 6, 2023, retrospective review was performed for personal demographics, clinical symptoms and treatment outcomes of 61 MN patients with COVID-19.Results There were 40 males and 21 females with a mean age of (51.7±12.8) year. And 31 cases (50.8%) achieved clinical remission and 51 (83.60%) had received two or more doses of COVID-19 vaccine. After an infection of COVID-19, MN patients presented multi-system clinical symptoms. Top three were fever (78.7%), cough & sputum (52.5%) and fatigue (44.3%). Twelve patients (19.7%) had only one symptom and 41 cases (67.2%) had three or more symptoms. According to the Diagnostic and Therapeutic Protocol for Novel Coronavirus Infection (Trial Edition X), 58 cases (95.1%) were mild and 3 (4.9%) medium. MN patients with COVID-19 were treated mostly symptomatically. Within 1 month after COVID-19, most patients stabilized. Only 1 case relapsed and another developed acute kidney injury. ConclusionsMN patients with COVID-19 have milder clinical symptoms and better short-term outcomes.

To investigate the effects of ultrasound treatment combined with pH adjustment on the gelling properties of composite gels prepared from ginkgo seed protein isolate (GSPI) and whey protein isolate (WPI), the physicochemical properties and molecular behavior of the GSPI and WPI mixture and the texture, water-holding capacity (WHC) and microstructure of the heat-induced gel of the protein mixture were characterized. Results showed that ultrasonic treatment at 25%, 45%, or 65% power alone did not significantly influence protein solubility. However, ultrasonic treatment combined with lower pH (pH 4.0) markedly decreased protein hydrophobicity, and increased surface sulfhydryl group content and the sol’s particle size. The results of sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and Fourier transform infrared (FTIR) spectroscopy showed that ultrasonic treatment affected protein structure and aggregation behavior. The heat-induced composite gel formed at lower pH had poor textural properties, whereas the composite gel formed at higher pH (especially at pH 5.0) exhibited improved textural properties and higher WHC. The textural properties and WHC of the composite protein gel were significantly improved with increasing ultrasonic power, which was attributed to the fact that ultrasonic treatment strengthened the interaction between hydroxyl groups in GSPI and WPI, promoting protein aggregation and cross-linking and improving the structural compactness of the composite gel. Overall, ultrasound treatment combined with pH adjustment could be an effective method for improving the functional properties of the composite protein gel.

[Introduction] In order to change the current patrol inspection mode of power plant mainly based on manual patrol ins-pection, improve the intelligent level of power plant patrol inspection, and fully grasp the design method of robot autonomous patrol inspection system. [Method] This paper started from the design idea of the robot autonomous patrol inspection system, defined the system functions, and gave the design scheme of the patrol inspection system from the hardware architecture and software functions. Based on this design scheme, a 5G+ robot autonomous patrol inspection system integrating progressiveness and practicality was designed. In addition, in order to make the robot's patrol inspection results more intuitively reflect the actual operation of the power plant, this paper, based on intelligent image processing technology, completed the analysis and processing of instrument readings, valves, button switch status and other information. [Result] The 5G+ robot autonomous patrol inspection system can realize map construction, task deployment, autonomous navigation and execution of patrol inspection tasks, automatic identification of patrol inspection equipment, and use 5G to return patrol inspection data, complete the analysis of patrol inspection results and automatically generate patrol inspection reports. [Conclusion] The 5G+ robot autonomous patrol inspection system in intelligent power plant developed in this paper can significantly improve the patrol inspection efficiency, improve the intelligent level of power plant operation and maintenance, provide strong technical support for the independent patrol inspection operation of the power plant, provide reliable basis for professionals to comprehensively grasp the operation status of the boiler room, steam turbine room, booster station and auxiliary workshop, and ensure the safe operation of equipment in key areas while reducing personnel and increasing efficiency, better assist the construction of smart power plants.

Objective To evaluate the forensic application value of an age estimation model based on DNA methylation in eastern Chinese Han population， and to provide a theoretical basis for exploring age estimation models suitable for different detection platforms. Methods According to the 6 age-related methylation sites in the published blood DNA methylation age estimation models of Chinese Han population， the DNA methylation level of 48 samples was detected by pyrosequencing and next-generation sequencing （NGS）. After submitting DNA methylation levels to the age estimation model， the DNA methylation ages were predicted and compared with their real ages. Results The 6 DNA methylation sites in both detection techniques were age-related， with an R2 of 0.85 and a median absolute deviation （MAD） of 4.81 years when using pyrosequencing； with an R2 of 0.84 and MAD of 4.41 years when using NGS. Conclusion The blood DNA methylation age estimation model can be used under pyrosequencing and multi-purpose regional methylation enrichment sequencing technology based on NGS and it can accurately estimate the age.

This is an essay in the field of metallurgical engineering. Using waste lithium iron phosphate battery cathode powder as raw material, a study on lithium selective leaching with Na2S2O8 as oxidant and low concentration acetic acid as aleaching agent was carried out. The effects of Na2S2O8 addition, acetic acid concentration, leaching temperature, leaching liquid-solid ratio and leaching time on selective leaching of lithium were investigated. At the conditions of acetic acid concentration of 0.3 mol/L, Na2S2O8 addition of 1.1 times the theoretical amount, L/S ratio of 10 mL /g and room temperature leaching for 2 h, the leaching rates of lithium, iron and phosphorus were 98.05%, 4.49% and 2.46% respectively.

Objective To strengthen the research capacity of professional Master students in clinical medicine,a new teaching mode of literature case presentation and discussion was tried in clinical epidemiology teaching and the teaching effectiveness was evaluated. Methods The professional Master students in clinical medicine of Peking Union Medical College who joined clinical epidemiology courses during 2019—2021 were selected as the research subjects(n=128). In 2019, the lecture-based teaching was adopted. In 2020 and 2021, a 4-hour literature case presentation and discussion class was added for the topic of “cohort study”. Feedback on the new teaching mode was collected through questionnaires from students taking the course in 2021. Results The average per-question score rate across all questions in the final examination for a student in 2020 was higher than that in 2019 (the median value was 84.7% vs 75.4%, P＜0.001). There was no statistical difference in the average per-question score rate across questions related to the cohort study topic for a student between the two years. Students′ score ratefor the case presentation and discussion in 2020 was positively correlated with the average per-question score rate for not only questions related to the cohort study topic but also all questions in the final examination(r=0.40, P＜0.01; r=0.31, P＜0.05). Among the students taking the course in 2021, 80% believed that they had achieved the knowledge-learning goal of this case discussion class. About 90% agreed that the teaching mode of literature case presentation and discussion was helpful in improving their ability in designing clinical research, reading literatures, presenting research work and carrying out team collaboration. All students were satisfied with this new teaching mode. Conclusions Conducting literature case presentation supplemented with discussion in clinical epidemiology teaching can help students better to Master clinical research methodology and enhance their comprehensive research capacity.

In order to alleviate the shortage of underground aggregate sources and time cost of vibration and compaction during backfilling, a new type of high-porous low-cementitious waste backfilling material (HPLCM), which was composed of self-compacting slurry and loose packing waste with high porosity, was developed to achieve the purpose of low cementitious backfilling. Uniaxial compression tests were carried out on HPLCM in the laboratory, using orthogonal strength variables of cement slurry and waste aggregate, to study their failure strength characteristics and statistical laws. Considering the combined impact of solid waste and slurry strength, a generalized strength model of shear fracture was established based on the fracture statistical results. The coupled strength utilization ratio of solid waste aggregate and self-compacted slurry was defined, and the optimal ratio and cost of mix proportion of HPLCM were analyzed. The results indicate that the uniaxial compressive strength and failure mode of HPLCM are jointly controlled by the component strength of self-compacting cementitious slurry and solid waste aggregate. The strength of both components has a positive correlation with the strength of HPLCM, which is limited by the weaker component, and when one component grows stronger, the failure mode will be dominated by the other component. The uniaxial compressive test of HPLCM presents shear failure modes, including shear planes in single oblique and cross oblique patterns with an average angle of 55.4°. The density of loose packing aggregates can be reduced from 85% to 58% by using the proposed backfilling material, and the strength of cementitious slurry can be optimized within the optimal mixing range to maximize the utilization of filling material strength when the strength of in-situ solid waste aggregates is obtained. Thus, the compaction time of solid filling and the amount of single gangue can be reduced.

γ- and δ-lactones are important flavor compounds, which are widely used in foods and daily chemical flavors. Due to the chiral centers in the molecules, the aroma characteristics of the two enantiomers of each of the lactones are quite different, which makes them have different purposes in the formulation of flavors and fragrances. In this paper, the natural sources, chemical structures, aroma characteristics and preparation methods of common chiral γ- and δ-lactones in foods are reviewed in order to provide a comprehensive understanding of chiral lactones and provide a reference for the development of new methods for their preparation.

Objective: To describe the characteristics of chest computed tomography (CT) findings of patients with severe COVID-19 complicated with other pathogens. Method: Chest CT data and outcomes of patients with severe COVID-19 complicated with other pathogens were retrospectively analyzed. Results: Twenty-seven patients were included in the study. Etiological examination showed that bacteria were isolated in 13 patients, fungi in 2 patients, and bacteria and fungi in the remaining 12 patients. Multiple lung lesions were found in the chest CT images of all 27 patients. Excluding the chest CT images of 6 patients with typical novel coronavirus pneumonia features, the remaining 21 cases mostly showed scattered or diffuse ground-glass, mixed density, patchy, and patchy-solid shadows distributed in the lung segments or lobes. Some of them were scattered in nodules or central lobular nodules, with the thickened interlobular septum showing "paving stone sign" and "vascular thickening sign" in the ground glass shadow, and air bronchial air images were visible in the solid shadows. Pleural effusion was found in most cases, with pulmonary air sacs in few cases, and mild lymph node enlargement in scattered cases. According to the outcomes, the patients were grouped into 6 patients who survived and 21 patients who died. The proportion of ground glass shadow and ground glass with solid shadow in the lung of the patients who died was higher than that of the patients who survived, and the other imaging findings were not statistically different. Conclusion: Secondary infections in patients with severe COVID-19 were mainly bacterial and fungal infections, with most infections were mixed pathogens. Chest CT images mainly showed ground glass, mixed density shadow, consolidation shadow, and nodular shadow without specific location distribution, and most cases were accompanied with pleural effusion, a few with lung sacs, and scattered cases with mild enlargement of chest lymph nodes, pavement stone sign, and vascular thickening sign. It showed the diverse imaging features of COVID-19 cases complicated with bacterial and fungal infections.

Objective To analyze the preliminary screening and follow-up testing data of HBV in Yantai area, and discuss the rationality of following up and re-entry program of HBV reactive blood donors. Methods Donors who were single reagent reactive by enzyme-linked immunosorbent assay (ELISA) in initial screening but non-reactive by nucleic acid testing (NAT) were followed up. Individual NAT(ID-NAT) was performed for HBV DNA, ELISA for HBsAg, HBsAb, HBeAb, HBeAg and HBcAb, and ECLIA for the detection of HBsAg. Results A total of 547 blood donors were HBsAg ELISA-/NAT+, and 97 were followed up, among which 24 met the requirements of re-entry while 73 did not. Of the 24 blood donors who met the re-entry requirements, 13 donated blood again, with test results all qualified. Conclusion The combination of ELISA, ID-NAT, and ECLIA methods for following up detection for HBsAg ELISA+ blood donors is recommended. Blood donors with HbsAb S/CO ≥ 10 and negative results for other tests met the re-entry requirements, with a re-entry rate at 24.74%, and the re-donation qualified rate of blood donors after re-entry was 100%.

Background Adequate gestational weight gain (GWG) is critical for maternal and child health. The Institute of Medicine (IOM) standard has long been adopted in clinical practice to guide GWG in China. Since October 2022, China has officially promulgated and adopted the Standard of Recommendation for Weight Gain during Pregnancy Period (WS/T 801-2022) (herein after referred to as SRWGPP) to guide GWG. Objective To compare the distribution of GWG recommended by the SRWGPP and IOM used for Chinese singleton pregnant women and associated adverse pregnancy outcomes, providing clinical evidence for further application of the SRWGPP. Methods The data of this study were from a prospective cohort study involving singleton pregnant women who gave birth in Beijing Obstetrics and Gynecology Hospital, Capital Medical University from May 2020 to September 2021 and participated in the Beijing Birth Cohort Study (registration number: ChiCTR220058395) . Baseline information was collected from the participants, and the incidence of pregnancy complications and outcomes was obtained from the clinical health record system. We compared the distribution of GWG of the participants based on the criteria by the SRWGPP and the IOM guidelines. Then we divided the participants into five groups: insufficient weight gain (IOM+IW) , insufficient weight gain+appropriate weight gain (IOM+IW+AW) , appropriate weight gain (IOM+AW) , appropriate weight gain+ excessive weight gain (IOM+AW+EW) , and excessive weight gain (IOM+EW) . The risk of adverse pregnancy outcomes〔large for gestational age (LGA) , small for gestational age (SGA) , macrosomia, low birth weight, and preterm birth〕 was analyzed after adjusting for confounding factors. Results A total of 11 839 singleton pregnant women were included. The proportions of women with insufficient, appropriate, and excessive GWG were 36.7% (4 339/11 839) , 38.9% (4 601/11 839) , and 24.5% (2 899/11 839) , respectively, according to the IOM standard, and were 16.2% (1 913/11 839) , 45.0% (5 332/11 839) , and 38.8% (4 594/11 839) , respectively, according to the SRWGPP. The proportions of pregnant women in groups of IOM+IW, IOM+IW+AW, IOM+AW, IOM+AW+EW and IOM+EW were 16.2% (1 913/11 839) , 20.5% (2 426/11 839) , 24.6% (2 907/11 839) , 14.3% (1 694/11 839) and 24.5% (2 899/11 839) , respectively. The results from multivariate Logistic regression analysis showed that the risk of overall adverse pregnancy outcomes in IOM+AW+EW group was higher than that in IOM+AW group〔aOR=1.23, 95%CI (1.07, 1.41) , P

【Objective】The impact of anaerobic disinfection (ASD) on the properties of tobacco field soil, major soil-borne diseases of tobacco, and their yield and quality is investigated by employing effective and pollution-free soil improvement techniques through the application of different organic materials.【Method】The field experiment wasconducted in two tobacco production areas in Dapu and Nanxiong, Guangdong Province. Three treatment groups of rice bran (MK), wood chips (MX) and rice bran mixed with wood chips (KX) were set up for soil anaerobic disinfection (ASD), and that without the addition of organic materials was used as CK, then soil properties, occurrence of major diseases and economic traits of tobacco were analyzed between different treatments.【Result】The results showed that ASD treatments with organic materials significantly increased soil pH value, soil enzyme activity and soil nutrient content in two production areas when compared with CK, while reduced soil EC value. In the Dapu production area, the mass fractions of soil organic matter, total nitrogen, available nitrogen, available phosphorus, and available potassium increased by 29.0%-40.6%, 7.0%-24.2%, 18.0%-24.4%, 25.9%-43.0%, and 37.5%-81.7%, respectively, compared to the control group (CK). The incidence rates of tobacco blue mold and black shank disease decreased by 5.8-17.3 and 7.5-15.8 percentage points, respectively, compared to CK. The disease severity index decreased by 34.7%-69.0% and 30.4%~68.9%, respectively, compared to CK. In the Nanxiong production area, the mass fractions of soil organic matter, total nitrogen, available nitrogen, available phosphorus, and available potassium increased by 0.1%-35.2%, 55.8%-89.4%, 19.7%-30.0%, 22.2%-27.5%, and 34.0%-76.3%, respectively, compared to CK. The incidence rates of tobacco blue mold and black shank disease decreased by 5.6-12.6 and 11.7-18.0 percentage points, respectively, compared to CK. The disease severity index decreased by 16.1%-63.7% and 43.4%-64.3%, respectively, compared to CK. In both production areas, the average tobacco yield per hectare increased by at least 1 949.25 yuan compared to CK. The proportion of high-grade tobacco in Dapu and Nanxiong production areas increased by 1.2~4.2 and 0.7-2.9 percentage points, respectively, compared to CK. The results of redundancy analysis showed that the incidence of bacterial wilt and black shank of flue-cured tobacco in Dapu was greatly affected by sucrase activity, and the incidence of flue-cured tobacco in Nanxiong was greatly affected by urease activity.【Conclusion】Adding MK (10 t/hm2) for ASD treatment in tobacco fields with frequent continuous cropping and severe soil-borne diseases. This treatment can effectively improve soil characteristics and enhance the quality of tobacco production.